Leishmania sp. amastigotes identification in canine transmissible venereal tumor

Leishmaniasis is a vector-borne disease with Leishmania chagasi being the etiological agent of canine visceral leishmaniasis in South America. Canine venereal tumor is a transplantable round cell tumor of histiocytic origin which is mostly observed in sexually active male and female intact dogs. It has been shown that Leishmania amastigotes have higher tropism for the canine male genital tract tissues and venereal leishmaniasis transmission has been documented in dogs but, to date, a canine venereal tumor-dependent transmission route has not been fully demonstrated. In this report, a 10-year-old, mixed breed, intact female dog presented a vaginal venereal transmissible tumor but no other clinical abnormalities otherwise. Unexpectedly, tumor tissue imprint smears examination revealed Leishmania sp. amastigotes within infiltrating macrophages. In addition to the cytological direct identification, the protozoan was confirmed within the neoplastic tissue by means of immunohistochemistry and polymerase chain reaction. This report illustrates an asymptomatic Leishmania sp. infection that may have started on or from the canine venereal tumor tissue, the latter option further supporting previous evidence of such an alternative vector-independent route of transmission for canine visceral leishmaniasis in areas where these diseases coexist.

CD95 (FAS) and CD178 (FASL) induce the apoptosis of CD4+ and CD8+ cells isolated from the peripheral blood and spleen of dogs naturally infected with Leishmania spp

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Drosophila melanogaster lipins are tissue-regulated and developmentally regulated and present specific subcellular distributions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Type of light in sand fly captures (Diptera:Psychodidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Clinical and parasitological evaluation of dogs naturally infected by Leishmania (Leishmania) chagasi submitted to treatment with meglumine antimoniate and allopurinol

Aiming to assess the efficacy of the treatment, to verify the occurrence of possible disease relapses and to search for the presence of parasites after the treatment, seven dogs naturally infected by Leishmania sp., were submitted to a treatment with meglumine antimoniate and allopurinol. For this, lymph node and bone marrow aspiration biopsies were carried out at seven moments. After the end of the six-month observation period all dogs were submitted to euthanasia. Then, spleen and liver “imprints” and in vitro cultures were carried out to search for amastigote forms of the parasite. All animals presented remission of the symptoms and during all the observation period no dog presented relapse of the disease, although amastigote forms of the parasite were observed in two of the animals at the end of the experiment. Thus, it was possible to conclude that the treatment promotes clinical healing but it does not eliminate the parasites completely.

Leishmania sp. infection in dogs from Florianópolis, Santa Catarina, SC, Brazil

The aim of the present study was to investigate the occurrence of Leishmania sp. infection in dogs (N = 491) living in the municipality of Florianópolis, Santa Catarina (SC), Brazil, which was considered a disease-free region for visceral leishmaniasis until 2011, when autochthonous cases of canine disease were notified. Seroprevalence in this population was assessed by ELISA (0.4%; 2/491) and IFAT (4.09%; 24/491). Only one dog exhibited seroreactivity in both serological methods, comprising a total of 25 (5.3%) seroreagent animals. Leishmania sp. DNA, obtained from a sample of whole blood of this animal, was amplified by both conventional and Real-Time PCR. Sequencing of the amplified DNA and, thereby, determination of the Leishmania species involved, was not possible. Our results suggest the necessity of a thorough epidemiological investigation in Florianópolis. (AU).

Níveis de carboidratos e lipídios em dietas para juvenis de tambaqui (Colossoma macropomum): variáveis produtivas, fisiológicas e turnover isotópico

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Registrar oralidades, analisar narrativas: sobre pressupostos da História Oral em Educação Matemática

The discussion about some features of the general framework which allows the use of Oral History in Mathematics Education is the main theme of this paper. Also the conception about what Oral History is, its theoretical baskgrounds, some of its purposes and the range of its results to Math Education research community are considered, as well the link between such method and the narratives, focusing what narratives are and how narratives can be analysed. A brief description of specific themes and researches developed in such approach, in Brazil, ends this article.

A cidade e o sanduíche: análise da capa do primeiro exemplar da revista Traço (1987)

This paper conducts an analysis of the cover of the first issue of Trace magazine, conceived and edited by a group of graphic artists from Bauru (SP) published in November 1987 from the study proposed by Donald Norman (2008) especially through the visceral, behavioral and reflective approaches. This is a critical exercise, based on the study, of the use and application of the theory of Norman, which focuses on the development and focusing the scope of graphic design.

Portal único (SILSTM) para ovário-histerectomia vídeo-assistida em cadelas

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Participação de gambás e cães domiciliados como reservatórios de Leishmania infantum e Trypanosoma cruzi georreferenciados nos municípios da Regional de Saúde de Três Lagoas - MS

Pós-graduação em Doenças Tropicais - FMB

Endovascular repair of a juxtarenal saccular aneurysm using the Multilayer Flow Modulator: report of the first case performed in a Public Hospital in Brazil

Endovascular aortic aneurysm repair (EVAR) involving renal and visceral arteries remains a great challenge. Several techniques have been developed over the time to treat juxtarenal, pararenal and thoracoabdominal aneurysms, highlighting the fenestrated and branched endografts, parallel prostheses as Chimney, Periscope and Sandwich Techniques and the use of flow modulation by multilayer stent. We report a case of saccular juxtarenal aortic aneurysm with high surgical risk for complex airway access due to a history of radical laryngectomy for laryngeal neoplasm. Due to chronic aorto-iliac obstructive disease, ostial stenosis of renal artery and limited diameter of the suprarenal aorta, we discarded options involving fenestrated/branched endografts and involving parallel prostheses techniques. We present this case as a therapeutic challenge and a successful treatment option in the short-term evaluation.

Indeterminate form of chagas disease and metabolic syndrome: a dangerous combination

Chagas’ disease (CD) has been a major concern in public health in Latin America countries and in Brazil there are about 3 million people suffering from this disease. With the social and economic changes which have been occurring in the last 6 decades in the country, there have been a lot of changes in the population life style with severe metabolic consequences, especially for those with Chagas' disease. The objective of this study was to evaluate the prevalence of metabolic syndrome in individuals with the indeterminate form of CD. A total of 74 individuals, mean age of 55.6 years, participated in the study. Anthropometric and biochemical evaluations were performed. Overweight/obesity was found in 86.5 % of individuals, increased waist circumference in 72.5%, and 67% had more than 30% of fat mass. Hyperglycemia and dyslipidemia were observed in 24.3% and 75.7% of patients, respectively. Metabolic syndrome was diagnosed in 48.2% of patients. The family history revealed high prevalence of cardiovascular diseases (80.3%), systemic arterial hypertension (57.1%) and diabetes mellitus (42.8%). A total of 90% of patients were overweight/obese, and it is well known that increased adipose tissue, specially visceral adipose tissue is highly associated with dyslipidemia and cardiovascular diseases, as well as imbalance in production of proinflammatory and antiinflammatory cytokines produced by that tissue. Adipocytes are also known as a reservoir for Trypanosoma cruzi, favoring an increase in parasite load and a possible reacutization of the disease. Therefore, the study individuals are at high risk of developing cardiovascular diseases as well as further symptomatic form of the Chagas' disease, mainlychagastic cardiopathy.

Estado nutricional e ocorrência de hipertensão arterial e de diabetes em idosos residentes e não residentes em instituições geriátricas

The aging process involves challenges in meeting social needs, economic and health. Physiopathological changes and socio-economic predispose the elderly population at nutritional risk. The monitoring of nutritional status predict some complications of obesity or malnutrition, and is a tool for health care. The objective to know the nutritional status and risk of chronic diseases of the elderly in geriatric institutions and volunteers. The elderly population of 3 geriatric institutions (n=122) and non-institutionalized elderly (n=75) were evaluated. The body mass index was evaluated according to Nutrition Screening Initiative. For waist circumference, was used the World Health Organization criteria. For statistical analysis, chi-square was used. A high proportion of obesity (46%) was observed. The underweight affects 4% and 20% of noninstitutionalized and institutionalized, respectively. The frequency of the risk of diseases associated with obesity was higher among non-institutionalized. Hypertension affects more than 50% of the elderly. The nutritional status was different between the two situations, with high incidence of overweight and visceral adiposity, in volunteers, and wasting among institutionalized. The data emphasize that it is necessary special attention and appropriate measures to prevent morbidity and mortality and to improve quality of life.

Expressão gênica de interferon-gama, fator de necrose tumoral alfa e interleucinas 2 e 5 no baço de gatos naturalmente infectados por Leishmania spp: Karina Yukie Hirata. -

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)