Building the sugarcane genome for biotechnology and identifying evolutionary trends

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of the effect of supplementing fermented milk with quinoa flour on probiotic activity

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desempenho e consumo de nutrientes em cordeiros alimentados com cana-de-açúcar hidrolizada sob diferentes condições

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Le(a-b-) phenotype: potential risk factor for infection by the RH strain of Toxoplasma gondii in pregnancy

The Lewis histo-blood group system is characterized by the expression of the Lea and Le(b) antigens in the gastrointestinal tract, whose synthesis results in interactions between alpha 2-L-fucosyltransferase (FUTII) and alpha 3/4-L-fucosyltransferase (FUTIII) enzymes coded by the FUT2 (19q. 13.3) and FUT3 (19p13.3) genes. FUTII and FUTIII fucosylate the type 1 oligosaccharide precursor (Gal beta 1 -> 3NAcGlc beta 1 -> 3-R) at distinct positions to form H type 1 (Fuc alpha 1. 2Gal beta 1. 3NAcGlc beta 1 -> 3-R) and Le(a) (Gal beta 1 -> 3[Fuc alpha 1 -> 4] NAcGlc beta 1 -> 3-R) antigens, respectively. The fucosylation of H type 1 antigens by FUTIII results in the Leb antigen (Fuc alpha 1. 2Gal beta 1. 3[Fuca1. 4] NAcGlc beta 1. 3-R). Thus, the presence of the FUTII and FUTIII enzymes leads to the expression of the Le(a+b+) phenotype, while the presence of only FUTIII allows the expression of the Le(a+b-) phenotype. The absence of the FUTIII enzyme leads to the expression of the Le(a-b-) phenotype, independent of the presence or absence of FUTII. Point mutations in FUT2 and FUT3 genes change the activity of these enzymes, impair the synthesis of Le(a) and Le(b) antigens, and contribute to the variability of Lewis phenotypes in the gastrointestinal tract. Toxoplasma gondii, an apicomplexan parasite that infects a large proportion of the world's population, utilizes the gastrointestinal tract as an infection route and seems to adhere to glycosylated molecules to invade human cells. These apparently independent events may be related. The aim of this study was to test the hypothesis that there is an association between the Lewis histo-blood group system and infection by T. gondii. Two hundred and nine serum samples collected from pregnant women were submitted to screening tests to detect anti-T. gondii antibodies, employing the indirect hemagglutination method. ELISA was utilized to identify IgG class anti-T. gondii antibodies specific for the RH strain. A hundred and ninety-five samples with concordant results for both methods were selected to form two groups: seropositive (G1) and seronegative (G2). The G428A mutation of the FUT2 gene, and T202C and C314T of the FUT3 gene, which allow inference of the gastrointestinal tract Lewis phenotypes, were identified using PCR-RFLP and PCR-SSP methods, respectively. Among the 195 samples selected, 116 (59.5%) were seropositive and 79 (40.5%) were seronegative. In G1, 68 (58.6%) were classified as Le(a+b+), 30 (25.9%) as Le(a+b-), and 18 (15.5%) as Le(a-b-), and in G2, 67 (84.8%) were classified as Le(a+b+), 12 (15.2%) as Le(a+b-), and 0 (0%) as Le(a-b-) (P < 0.0001). The Le(a-b-) phenotype is associated with a high risk of RH strain T. gondii infection when compared with the Le(a+b+) [P = 0.0001; OR = 36,460; 95%CI = 2.152-617,680] and Le(a+b-) phenotypes [P = 0.0118; OR = 15,165; 95%CI = 0.8463-271,710]. The Le(a+b-) phenotype showed a higher risk compared to the Le(a+b+) phenotype [P = 0.0206; OR = 2463; 95%CI = 2463-5214]. The results suggest that the Le(a-b-) phenotype is strongly associated with a greater risk of infection by the RH strain of T. gondii compared to the other phenotypes. It is possible that the absence of fucosylation of the type 1 oligosaccharide precursor as well as the variations in the structures of the Le(a) and Le(b) antigens influence susceptibility to infection by this parasite.

Características da cana-de-açúcar in natura e hidrolisada com cal virgem em diferentes tempos de estocagem

Our objective was to evaluate the chemical composition and in vitro digestibility of dry matter of hydrolyzed sugar cane in different storage times. We conducted the trial in the Forage Sector at UNIFENAS, campus of Alfenas (MG) in 2010. The data were analyzed as completely randomized design in factorial scheme 2 (sugar cane without lime and hydrolyzed with 1.0% of lime, basis of fresh matter) x 6 (six times of storage: 0, 24, 48, 72, 96 and 120 hours), with three replicates. The sugar cane stored without lime showed lower dry matter (DM), crude protein, neutral detergent fiber and acid detergent fiber contents compared to hydrolyzed sugar cane. However, the hydrolyzed sugar cane showed higher contents of organic matter (OM) and total digestible nutrients. We reported higher in vitro digestibility of DM and OM when the sugar cane was not hydrolyzed. This can be explaining by better chemical composition of sugar cane without lime. The storage of sugar cane without lime shows results more interesting because the chemical composition and digestibility is better than hydrolyzed sugar cane. So, we not recommended using lime on the sugar cane. Moreover, the sugar cane without lime can be stored until 96 hours after cut.

Identificação de bactérias láticas e otimização da produção de ácido lático

O objetivo deste trabalho foi identificar o micro-organismo Y15C previamente isolado no laboratório de microbiologia industrial, da UNESP de Rio Claro, utilizando técnicas bioquímicas, além de otimizar a produção de ácido lático por meio de fermentação batelada simples e batelada alimentada. Considerando o potencial do micro-organismo para a produção de D(-) ácido lático, com 100% de pureza, isômero usado na síntese de polímeros empregados na produção de diversos materiais resistentes e biodegradáveis, foi feita a otimização do meio de cultura, tendo como fonte de carbono a manipueira. A manipueira por ser um resíduo do processamento da mandioca que contém grande quantidade de amido e quando hidrolisado pode ser uma fonte de carbono de baixo custo, quando utilizado pelo micro-organismo. O isolado Y15C foi identificado como Lactobacillus delbrueckii ssp delbrueckii, por meio de testes bioquímicos. Em relação à otimização dos parâmetros de fermentação, usando planejamento experimental, observou-se que a adição de componentes ao meio de cultivo, assim como a variação da temperatura não aumentou significamente a produção de ácido lático. Desta forma optou-se por trabalhar com as menores concentrações de AMM (1,6 mL/L) e citrato (0,96 g/L) e maior temperatura (43,4ºC). Para que haja aumento na produção de ácido lático podem ser realizados diversos tipos de fermentações, no caso deste trabalho foram comparados dois tipos de fermentação, batelada simples e batelada com alimentação constante. A maior concentração de ácido lático (41,6 g/L) foi observada que quando utilizada fermentação do tipo de fermentação em batelada... (Resumo completo, clicar acesso eletrônico abaixo)

Marcha de acúmulo de matéria-seca e de nutrientes pelo milho superdoce

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Diversidade bacteriana em solos, vinhaça e semicompostagem relacionados ao cultivo de cana-de-açúcar

Pós-graduação em Microbiologia Agropecuária - FCAV

Transtornos reprodutivos causados por agentes infecciosos em animais de canis comerciais da microrregião de Ribeirão Preto, estado de São Paulo

Pós-graduação em Medicina Veterinária - FCAV

Produção de bioetanol a partir da fermentação de caldo de sorgo sacarino e cana-de-açúcar

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Interaction between N-fertilizer and water availability on borer-rot complex in sugarcane

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Efeitos da fertirrigação sobre a ocorrência e danos de Mahanarva fimbriolata (Stål) (Hemiptera: Cercopidae) em cana-de-açúcar

Brazil stands out as the world's largest producer of sugarcane and its productivity is closely related to the availability of water and nitrogen during its development and also to the incidence of pest insects. Among the species most harmful to the sugarcane in Brazil, Mahanarva fimbriolata stands out, which has seriously endangered the yields. In this context, the present study aimed to evaluate the effect of the nitrogen fertirrigation on the incidence and damage caused by M. fimbriolata in sugarcane. The experiment was composed of five treatments and four replications in a randomized block design (RBD). The treatments were composed of four doses of N-fertilizer and control (0, 50, 100, 150, 200 kg.ha-1) in drip irrigation system. The parameters evaluated were: number of nymphs in the roots, stalk productivity, sugar content, percentage of sugar, total recoverable sugar and the agricultural contribution margin. It was observed that the fertirrigation increases the productivity of stalks and sugar with the increase of nitrogen fertilization by irrigation. The use of 200 kg.ha-1 of N-fertilizer increased the incidence of M. fimbriolata up to the control and economic damage levels. The biggest financial yield was obtained with the dose of 150 kg.ha-1 of N-fertilizer.

Caracterização das espécies de leishmania em sangue periférico de cães por PCR-RFLP NA área endêmica de Bauru/SP

Pós-graduação em Ciência Animal - FMVA

Variabilidade de atributos físicos de um latossolo vermelho sob cultivo de cana-de-açúcar

The goal of this paper was to select among the attributes surveyed soil, one with better representation to explain the variability of the technological components of sugar cane. The study was conducted at ETH Eldorado Plant in Rio Brilhante, MS, in the agricultural year 2011/2012, in a Oxisol, which was installed a geostatistical grid for data collection of soil and plant, with 80 sampling points, a 80 ha area. From the standpoint of linear and spatial TCH has been explained as a function of volumetric moisture. The volumetric moisture collected at a depth of 0.00 to 0.20 m, which had values between 0,24 to 0,270 m(3) m(-3), resulted in sites with the highest productivity of sugar cane per hectare from 64 to 70 t ha(-1). To aid future studies aimed at precision agriculture, which will use the same attributes as those of the future works, the values of ranges of spatial dependence to be used should be between 81 and 487 meters.

Acúmulo de nutrientes e decomposição do palhiço de cana em função de doses de vinhaça

Understanding the influence of vinasse application on trash decomposition and its effects on soil chemical attributes and sugar cane culture is essential for the good management of the production system. The objective of this study was to assess the time of decomposition and nutrient cycling of the green ratoon cane's trash, and their effects on soil fertility and technological components of crops, in terms of vinasse doses. The study was developed in the northwest region of Sao Paulo State - Brazil, under Eutrophic Ultisol, using the RB855453 variety. The experimental design was randomized blocks with split plot in time. The treatments were composed of four doses of vinasse (0, 50, 100 and 200 m(3) ha(-1)). There was a 20.9% reduction in the amount of crop residue remaining after one year review. The vinasse doses increased the ratoon cane's yield, without interfering in the raw material quality. The green cane system, in which the trash is deposited on the soil and vinasse application, changes the dynamics of the trash decomposition and modifies the soil fertility, contributing positively to the crop yield.