Efeito do cobre na atividade da enzima pirogalol peroxidase em plantas de Myriophyllum aquaticum cultivadas em solução nutritiva

Myriophyllum aquaticum é uma planta perene, herbácea, que pode se desenvolver totalmente submersa ou com a porção terminal dos ramos acima da superfície da água. É também considerada uma planta daninha que possui elevado potencial de colonização, o qual, dependendo da densidade populacional, pode causar aumento no teor de matéria orgânica e redução de oxigênio na água, comprometendo a qualidade da água e seus usos múltiplos. O objetivo do presente trabalho foi verificar a influência do cobre na atividade da pirogalol peroxidase de plantas de M. aquaticum submetidas à solução nutritiva contendo concentrações de cobre de 1,2; 11,2; 21,2; 31,2; e 41,2 µg L-1. O experimento foi conduzido em um delineamento experimental inteiramente casualizado, com quatro repetições e cinco tratamentos, aos quais as plantas foram submetidas durante 21 dias. Aos 81 dias após a instalação das mudas em solução nutritiva contendo os diferentes níveis de cobre, as folhas foram colhidas a partir do ápice da planta até o final do ramo, que não estavam em contato com a solução. Esse material fresco foi envolvido por plástico transparente e papel-alumínio e, a seguir, congelado em nitrogênio líquido, sendo armazenado em freezer a -20 ºC até o momento da determinação da atividade da enzima pirogalol peroxidase. A atividade da enzima foi progressiva com o aumento das doses de cobre. As plantas cultivadas com 40 µg L-1 de Cu2+ após três semanas, com base em avaliação visual, apresentaram redução no desenvolvimento.

PCR associated with agar gel immunodiffusion assay improve caprine arthritis-encephalitis (CAEV) control

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Anthelmintic resistance in cattle nematodes in northwestern São Paulo state, Brazil

The present study was carried out in the northwestern region of São Paulo State, Brazil, to determine the anthelmintic resistance status in cattle naturally infected with gastrointestinal nematodes. The anthelmintics tested were levamisole phosphate (Ripercol (R), Fort Dodge), albendazole sulphoxide (Ricobendazole, Fort Dodge), ivermectin (Ivomec (R), Merial) and moxidectin (Cydectin (R), Fort Dodge), administered at the doses recommended by the manufacturers. From April 2002 to May 2004, 25 cattle farms were evaluated. on each farm, steers were divided into treatment and control (not treated) groups based on fecal egg counts (FEC). Between 7 and 10 days after the anthelmintics administration, fecal samples were collected from each animal for post-treatment FEC. Fecal cultures from each group were also prepared for larval identification. After treatment, mean FEC reduction (FECR) in treatment groups (compared with control groups) was assessed on each farm. FECR was lower than 90% on 23 farms after ivermectin treatment. on 19 farms, FECR of 100% was recorded following moxidectin treatment; on the remaining 6, FECR ranged from 90% to 97.2%. After albendazole treatment, FECR was higher than 90% on 20 farms and ranged from 47.4% to 84.6% on other 5. After levamisole treatment, FECR was higher than 90% on 23 farms and equal to 47.4% and 73.7% on other 2 farms. Results indicated the presence of resistant Cooperia spp. and Haemonchus spp., especially to ivermectin; on some farms, resistance to albendazole and levamisole was also observed. (C) 2007 Elsevier B.V. All rights reserved.

Laser treatment of experimentally induced chronic arthritis

In this work, we investigated the effects of He-Ne laser irradiation on the inflammatory process induced in the articular cartilage of the right knee of guinea pigs. Through electron microscopy analysis it was possible to identify the induced arthritis in the articular cartilage and its modification after the laser treatment. The laser radiation promoted a reduction in the proliferation of the inflammatory cells in the damaged tissue and also induced the formation of cartilage bridges that tied the destroyed parts favoring the formation of a repaired tissue in the injured cartilage. (C) 2000 Elsevier B.V. B.V. All rights reserved.

Culture-dependent and culture-independent characterization of microorganisms associated with Aedes aegypti (Diptera: Culicidae) (L.) and dynamics of bacterial colonization in the midgut

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Seasonal effects on the relationship between photosynthesis and leaf carbohydrates in orange trees

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Bioconcentration of Cd and Pb by the River Crab Trichodactylus fluviatilis (Crustacea: Decapoda)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Relation of pretreatment sequence diversity in NS5A region of HCV genotype 1 with immune response between pegylated-INF/ribavirin therapy outcomes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Streptococcus mutans Biofilm Adhesion on Composite Resin Surfaces After Different Finishing and Polishing Techniques

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Cytotoxicity and Genotoxicity of Root Canal Sealers Based on Mineral Trioxide Aggregate

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Supersymmetric 3-3-1 model with right-handed neutrinos

We consider the supersymmetric extension of the 3-3-1 model with right-handed neutrinos. We study the mass spectra in the scalar and pseudoscalar sectors, and for a given set of the input parameters, we find that the lightest scalar in the model has a mass of 130 GeV and the lightest pseudoscalar has mass of 5 GeV. However, this pseudoscalar decouples from the Z(0) at high energy scales since it is almost a singlet under SU(2)(L)circle timesU(1)(Y).

L-R asymmetries and signals for new bosons

Several left-right parity violating asymmetries in lepton-lepton scattering in fixed target and collider experiments are considered as signals for doubly charged vector bosons (bileptons).

Supersymmetric 3-3-1 model

We build a complete supersymmetric version of a 3-3-1 gauge model using the superfield formalism. We point out that a discrete symmetry, similar to R symmetry in the minimal supersymmetric standard model, is possible to be defined in this model. Hence we have both R-conservina and R-violating possibilities. Analysis of the mass spectrum of the neutral real scalar fields show that in this model the lightest scalar Higgs boson has a mass upper limit, and at the tree level it is 124.5 GeV for a given illustrative set of parameters.

Lepton masses in a supersymmetric 3-3-1 model

We consider the mass generation for both charginos and neutralinos in a 3-3-1 supersymmetric model. We show that R-parity breaking interactions leave the electron and one of the neutrinos massless at the tree level. However, the same interactions induce masses for these particles at the 1-loop level. Unlike the similar situation in the minimal supersymmetric standard model, the masses of the neutralinos are related to the masses of the charginos.

The possibility of discovering new boson in e(-)e(-), mu(-)mu(-), e(-)mu(-) colliders

Several left-right asymmetries in Moller (electron-electron), muon-muon and electron-muon scattering are considered in the context of the electroweak standard model and in a model with SU(3)(C) x SU(3)(L) x U(1)(Y) gauge symmetry at tree level in collider experiments. We show that these asymmetries are very sensitive to a doubly charged vector bilepton in the case of ee and mu mu colliders and to an extra Z' neutral vector boson contribution in e(-)mu (-) collider.