Establishment of a PCR and restriction enzyme assay for the detection of the hemochromatosis gene in normal subjects and diabetic patients

The purpose of this study was to establish a polymerase chain reaction (PCR)-restriction enzyme assay for detecting the hereditary hemochromatosis (HHC) mutation, C282Y, in gestational and gestational diabetic subjects in South Florida. DNA samples from 43 gestational subjects were amplified by PCR, digested with RsaI, and analyzed by electrophoresis. An allelic frequency of 2.33%, or 4.65% heterozygosity, was observed. The assay is successful and applicable to future studies on HHC and gestational diabetes. ^

Author Pat Warren at her study and work house away from main house

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Pat Swinton and Ti-Grace Atkinson

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Pat Swinton

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Pat McQuillan, founder of Catholics for Choice demonstrates in front of St. Patrick's Cathedral for abortion demonstration

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Demonstration in support of Pat Swinton

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Pat Swinton at a demonstration outside of Federal Plaza

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Pat Swinton at Federal Court House

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Pat Collins, CBS-TV afternoon program

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Author Pat Warren

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Ms. Magazine editor Pat Carbine, Donna de Varona, Bella Abzug, Wilma Rudolph, Althea Gibson during the Ms. Mile Event at Madison Square Garden

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Pat Moore poses with her book

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MS. magazine's 10th anniversary party with Gloria Steinem (left) cutting the cake with editor Pat Carbine

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MS. Magazine editor Pat Carbine speaks at Women's Political Caucus meeting

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Ms. Magazine Mile track event at Madison Square Garden with Pat Carbine, Donna Da Varona, Althea Gibson, Bella Abzug, and Wilma Rudolph

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