In Vivo Binding and Hierarchy of Assembly of the Yeast RNA Polymerase I Transcription Factors

Transcription by RNA polymerase I in Saccharomyces cerevisiae requires a series of transcription factors that have been genetically and biochemically identified. In particular, the core factor (CF) and the upstream activation factor (UAF) have been shown in vitro to bind the core element and the upstream promoter element, respectively. We have analyzed in vivo the DNAse I footprinting of the 35S promoter in wild-type and mutant strains lacking one specific transcription factor at the time. In this way we were able to unambiguously attribute the protections by the CF and the UAF to their respective putative binding sites. In addition, we have found that in vivo a binding hierarchy exists, the UAF being necessary for CF binding. Because the CF footprinting is lost in mutants lacking a functional RNA polymerase I, we also conclude that the final step of preinitiation-complex assembly affects binding of the CF, stabilizing its contact with DNA. Thus, in vivo, the CF is recruited to the core element by the UAF and stabilized on DNA by the presence of a functional RNA polymerase I.

Dynamic properties of the Ras switch I region and its importance for binding to effectors

We have investigated the dynamic properties of the switch I region of the GTP-binding protein Ras by using mutants of Thr-35, an invariant residue necessary for the switch function. Here we show that these mutants, previously used as partial loss-of-function mutations in cell-based assays, have a reduced affinity to Ras effector proteins without Thr-35 being involved in any interaction. The structure of Ras(T35S)⋅GppNHp was determined by x-ray crystallography. Whereas the overall structure is very similar to wildtype, residues from switch I are completely invisible, indicating that the effector loop region is highly mobile. 31P-NMR data had indicated an equilibrium between two rapidly interconverting conformations, one of which (state 2) corresponds to the structure found in the complex with the effectors. 31P-NMR spectra of Ras mutants (T35S) and (T35A) in the GppNHp form show that the equilibrium is shifted such that they occur predominantly in the nonbinding conformation (state 1). On addition of Ras effectors, Ras(T35S) but not Ras(T35A) shift to positions corresponding to the binding conformation. The structural data were correlated with kinetic experiments that show two-step binding reaction of wild-type and (T35S)Ras with effectors requires the existence of a rate-limiting isomerization step, which is not observed with T35A. The results indicate that minor changes in the switch region, such as removing the side chain methyl group of Thr-35, drastically affect dynamic behavior and, in turn, interaction with effectors. The dynamics of the switch I region appear to be responsible for the conservation of this threonine residue in GTP-binding proteins.

Reliability and Validity Evidence of Scores on the French Version of the Questionnaire about Interpersonal Difficulties for Adolescents

This study examined the reliability and validity evidence drawn from the scores of the French version of the Questionnaire about Interpersonal Difficulties for Adolescents (QIDA) in a sample of 957 adolescents (48.5% boys) ranging in age from 11 to 18 years (M = 14.48, SD = 1.85). A principal axis factoring (PAF) and confirmatory factor analyses (CFA) were performed to determine the fit of the factor structure of scores on the QIDA. PAF and CFA replicated the previously identified correlated five-factor structure of the QIDA: Assertiveness, Heterosexual Relationships, Public Speaking, Family Relationships, and Close Friendships. The QIDA yielded acceptable reliability scores for French adolescents. Validity evidence of QIDA was also established through correlations with scores on the School Anxiety Inventory and the Social Anxiety Scale for Adolescents. Most of the correlations were positive and exceeded the established criteria of statistical significance, but the magnitude of these varied according to the scales of the QIDA. Results supported the reliability and validity evidence drawn from the scores of the French version of the QIDA.

Catharine of Russia ; or, The child of the storm : an historical drama, founded on an incident in the life of Peter the Great.

Separated from some unknown collection.

A memoir of the life of Peter the Great.

Mode of access: Internet.

Constantine the Great. The union of the state and the church.

"Published under the direction of the Tract committee."

Letter from the Governor of the state of Ohio to the President of the Senate transmitting a report of the Joint Committee of both houses of the General Assembly, on the communication of the Auditor of state upon the subject of the proceedings of the Bank of the United States against the officers of state in the United States Circuit court, February 1, 1821 ...

Mode of access: Internet.

Exercises to the rules and construction of French speech; consisting of passages extracted out of the best French authors, with a reference to the grammar-rules, to be turned back into French.

With his Grammar of the French tongue. London, 1801.

A memoir of the life of Peter the Great.

Published anonymously.

Ideas napoleonicas. Aumentadas con notas relativas á Espana, un diálogo entre Napoleon y Robespierre y las coversaciones de Canova.

Mode of access: Internet.

History of the war in Spain and Portugal, from 1807 to 1814 /

"Biographic memoir of the French Marshal Soult, duke of Dalmatia"--p.[345]-375.

A memoir of the life of Peter the Great.

Ascribed also to Richard Alfred Davenport.

A memoir of the life of Peter the Great /

Mode of access: Internet.

History of the first Council of Nice: a world's Christian convention,

Mode of access: Internet.

French dramatists of the 19th century /

A brief chronology of the French drama in the nineteenth century.--The romantic movement.--Victor Hugo.--Alexandre Dumas.--Eugène Scribe.--Emile Augier.--Alexandre Dumas fils.--Victorien Sardou.--Octave Feuillet.--Eugène Labiche.--Meilhac and Halévy.--Emile Zola and the present tendencies of French drama (1881)--A ten years' retrospect: 1881-1891.