Método e kit para identificação genética humana por meio de polimorfismos do DNA mitocondrial para aplicação em populações miscigenadas

A presente invenção refere-se a um método e kit para identificação genética humana por meio da análise de polimorfismos específicos do DNA mitocondrial para aplicação em populações miscigenadas como a brasileira, por exemplo. A técnica desenvolvida, além de permitir a identificação do indivíduo, permite també classificá-lo em halogrupos do DNA mitocondrial possibilitando a identificação da origem ancestral materna do indivíduo testado. A referida invenção pode ser aplicada na área de genética forense, pela polícia científica ou por laboratórios particulares, tendo como principais beneficiados populações miscigenadas que não dispõem de técnicas específicas para sua identificação e classificação genética.

Processo de modificação genética de bactéria acidófilas e construção de um vetor de transformação

The present invention describes a method for transforming chemolithotrophic acidophilic bacteria using electroporation technology. The proposed method allows transforming a bacterial line using a transformation vector, the pAF vector, which contains an origin of vegetative replication that allows the vector to replicate inside the bacteria without altering the natural physiological functions of the latter. Also disclosed is the use of the bacteria modified according to the invention in bioleaching processes of sulphated copper, gold, uranium, nickel, zinc and cobalt ore, inter alia.

Diversidade genética em coleções amazônicas de germoplasma de cupuaçuzeiro [Theobroma grandiflorum (Willd. ex Spreng.) Schum.]

This study aimed to evaluate the genetic diversity and relatedness of 24 accessions of Theobroma grandiflorum, originating from three units of Embrapa, aiming their use as parents in hybridization specie programs. The genetic markers used were heterologous microsatellite loci developed for cocoa. In the population studied 45 alleles were found. The effective average number of alleles per locus (2.33) was less than the average number of alleles per locus (3.21), indicating that many alleles have low frequency. The observed heterozygosity at polymorphic loci ranged from 0.33 to 1.00 with a mean of 0.54 and expected heterozygosity ranged from 0.48 to 0.76 with a mean of 0.54. The fixation index medium between loci (0.003) was not significantly different from zero. The estimate of relatedness between pairs of individuals indicates that some may be relatives, including half-brothers and clones. The results suggest that the accesses of T. grandiflorum analyzed contain a moderate level of genetic diversity and absence of inbreeding and therefore great potential for use in breeding programs.

Leucograma e metabolismo oxidativo de neutrófilos em cabras da raça Saanen nos períodos de gestação, parto e pós-parto

The purpose of this study was to evaluate the leukocyte count and the oxidative metabolism of neutrophil in Saanen goats during periods of pregnancy, parturition and postpartum. Were used 20 Saanen goats, clinically healthy and serologically negative for caprine arthritis encephalitis virus (CAEV). Blood samples were collected by jugular venipuncture 49 (M1), 42 (M2), 35 (M3), 28 (M4), 21 (M5), 14 (M6), seven (M7), three (M8) days before the parturition, on the day of birth (M9), three (M10) and seven (M11) days postpartum, for the leukocyte count, and serum for cortisol, estradiol and progesterone determination. From 28 days (M4) before parturition until seven days postpartum (M11) blood samples were collected for evaluation of oxidative metabolism of neutrophils by the nitroblue tetrazolium reduction test (NBT). The results showed that at parturition day there were an increase in cortisol and estradiol levels and a decrease in progesterone serum, neutrophilic leukocytosis and left shift slight, decrease of lymphocytes, increase in the neutrophil: lymphocyte, eosinopenia, monocytosis and basophilia. There was a neutrophilic leukocytosis and an increase in the neutrophil: lymphocyte on the seventh day postpartum. There were not significant alterations in oxidative metabolism of neutrophils during pregnancy, parturition and postpartum. It was concluded that parturition causes an elevation in cortisol and estradiol levels and a decrease in progesterone serum determining a neutrophilic leukocytosis and left shift slight, with a reduction of lymphocytes, increase in the neutrophil:lymphocyte, eosinopenia, monocytosis and basophilia. Neutrophilic leukocytosis, increase in the neutrophil: lymphocyte and fibrinogen are detected on the seventh day postpartum. Pregnancy, parturition and the postpartum do not change the oxidative metabolism of neutrophils evaluated by NBT reduction test.

Variação genética para caracteres silviculturais em progênies de polinização aberta de Astronium graveolens Jacq. (Anacardiaceae)

This study has investigated the genetic variation for silvicultural traits in an open-pollinated progeny test of Astronium graveolens Jacq., established at Luiz Antônio Experimental Station (State of São Paulo, Brazil). The trial was planted in a random block experimental design, containing 23 families, six replications and five plants per plot. The traits measured were diameter at breast height (DBH), total height and stem form. The assessments were taken at the age of 19 years. Significant differences were not detected by the analysis of variance, suggesting that the genetic variation was low, as well as the probability to raising genetic gains through selection among progenies. The coefficient of genetic variation was moderate for the traits height (8.2%) and DBH (21.2%) and low to stem form (4.0%). However, the average coefficient of heritability among progenies was low for all studied traits (ranging from 0.02 to 0.15), confirming the low probability of genetic improvement of this population by selection among progenies.

Metabolismo oxidativo e cardíaco de equinos submetidos a exercício de baixa intensidade antes e após suplementação com antioxidante

The present study aimed to evaluate cardiac and lipoperoxidation markers in horses subjected to low intensity and long duration (TLD) exercise test, before and after vitamin E supplementation. For this purpose, 10 horses were used, subjecting them to the first TLD with a workload based on individual maximal oxygen uptake (VO2max). Then, horses received vitamin E (dl-alpha-tocopherol) during 59 days at a daily oral dose of 1,000IU, and thereafter they performed a second TLD with the same protocol as the first. Blood samples were collected to determine plasma malondialdehyde (MDA) as an index of lipoperoxidation, serum cardiac troponin I (cTnI) and creatine kinase MB isoenzyme (CK-MB) as cardiac markers. As a result of the exercise, there was no significant increase in MDA or cTnI, but serum CK-MB increased suggesting myocardial stress. The supplementation was able to minimize reactive oxygen species production, as evidenced by lower concentrations of MDA at all times evaluated, but it didn't cause protective effect on the myocardium. It was concluded that the low intensity and long duration exercise promoted light myocardial stress in horses and vitamin E supplementation reduced lipoperoxidation.

Qualidade de sementes, teor de nitrogênio e expressão gênica em genótipos de milho inoculados com Azospirillum brasilense

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Abordagem genética e multivariada na performance agronômica de genótipos de soja oriundos de diferentes genealogias

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Influência do resveratrol sobre metabolismo energético, estresse oxidativo e expressão SIRT1 no miocárdio de ratos diabéticos

Pós-graduação em Ciências Biológicas (Farmacologia) - IBB

Efeito do exercício físico em ratos submetidos à dieta padrão restrita no tempo de ingestão e sacarose: metabolismo energético e estresse ocidadtivo

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Variabilidade genética e variação das regiões heterocromáticas em linhagens de Triatoma infestans (Heteroptera : Reduviidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Dinâmica do metabolismo micromolecular da cana-de-açúcar em resposta à infecção com o fungo Puccinia kuehnii (ferrugem alaranjada)

Pós-graduação em Química - IQ

Variabilidade genética e seleção de progênies de Eucalyptus Benthamii maiden et cambage

Pós-graduação em Ciência Florestal - FCA

Estudo dos impactos do corte seletivo de árvores na diversidade genética e demografia de população de araucaria angustifolia, utilizando modelagem ecogene

Pós-graduação em Agronomia - FEIS

Metabolismo da videira ‘syrah’ no semiárido nordestino sob três estratégias hídricas

The almost complete absence or misdistribution of water as a natural resource frequently constitute a limiting factor for plant growth and development in the semi-arid northeastern Brazil. In this context, the use of appropriate irrigation techniques is an essential and indispensable factor for proper functioning of the primary and secondary metabolisms in plants. This study aimed to assess the metabolism of the Syrah grapevine in the semi-arid northeastern Brazil, by using three irrigation strategies (controlled deficit irrigation [CDI], deficit irrigation [DI], and full irrigation [FI]). The research was conducted at Embrapa Semiarid (Embrapa-Brazilian Agricultural Research Corporation), Experimental Field of Bebedouro, municipality of Petrolina, state of Pernambuco, Brazil. The statistical design was randomized blocks, with a 3 x 6 factorial scheme. Six samplings were performed throughout the experiment cycle at 54, 60, 73, 87, 101, and 115 days after pruning (DAP). We analyzed the total soluble sugars, reducing sugars, total soluble protein, and invertase activity. Analysis of variance and the F test were performed for all analyzed variables. The means were compared using the Tukey test at 5% significance. At the end of the experiment cycle, DI was found to increase the average acid invertase activity in the plant cell wall. Further, by the end of the experiment cycle, the total soluble sugars and reducing sugars increased in all plants in the three irrigation strategies. Thus, we conclude that it is possible to reduce water consumption in vineyards of the semi-arid northeastern Brazil, without significantly affecting the protein and sugar metabolisms in the plants.