Resposta comportamental da broca-do-café Hypothenemus hampei (Ferrari, 1867) (Coleoptera: Curculionidae: Scolytinae) a voláteis de frutos de café

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Dinâmica populacional de insetos fitófagos e predadores em cana-de-açúcar sob aplicação de silício

Pós-graduação em Agronomia (Entomologia Agrícola) - FCAV

Efeitos do pneumoperitônio e de uma manobra de recrutamento alveolar seguida por pressão positiva no final da expiração na função cardiopulmonar em ovinos anestesiados com isoflurano e fentanil

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Trehalose and a calcium chelator for ram semen cryopreservation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Teor e acúmulo de nutrientes em grãos de feijão comum em semeadura direta, sob déficit hídrico

O feijão comum possui notória importância socioeconômica, constitui uma das mais importantes fontes proteicas da dieta brasileira e juntamente com o arroz, proporciona uma dieta mais vantajosa e equilibrada em termos de aminoácidos. O objetivo desse trabalho foi avaliar o efeito das combinações de diferentes reposições hídricas nas fases vegetativa - I e reprodutiva - II, durante o ciclo do feijoeiro IAC Alvorada, e comparar o teor nutricional dos grãos. Foi utilizado o delineamento em blocos casualizados, com quatro repetições em fatorial 4 x 4, sendo quatro níveis de déficit hídrico 100, 80, 60 e 40% da evapotranspiração da cultura (ETc) e os mesmos níveis foram repetidos em duas fases de desenvolvimento da cultura do feijão (fases I e II). Cada parcela possuía as dimensões de 4 m x 1,8 m, totalizando 7,2 m2 de 0,45 m. Inicialmente irrigou-se pelo sistema aspersão convencional em um período de 60 minutos diariamente, posteriormente o sistema adotado foi por gotejamento, conforme cada tratamento de restrição hídrica. Avaliaram-se os teores nutricionais de macro (N, P, K, Ca, Mg e S) e micronutrientes (B, Cu, Fe, Mn e Fe). A interação entre as lâminas nas fases I e II influenciou nos teores de micronutrientes Fe e S em grãos de feijão. Houve aumento dos teores de Fe e diminuição dos teores de Cu, Mn e B com a deficiência hídrica em uma das fases. Os macro e micronutrientes mais extraídos foram N, P, K, Fe, B e Mn. O N e o P são os nutrientes exportados em maior quantidade pelos grãos.

Atividade da enzima nitrato redutase em cultivares de arroz de terras altas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Pruning management of cassava for animal feeding: parameters of the root

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Desempenho agronômico e produtivo de cultivares de soja em diferentes ciclos e safras

With the objective of gathering technical data about soybean cultivars performance in Botucatu, state of São Paulo, Brazil, an experiment was conducted to evaluate seventeen genotypes. The experiment was conducted during the Summer seasons of 2002/03, 2003/04, and 2004/05. The experiment was set in the field according to a complete block design with four repetitions. The soybean cultivars were ‘Embrapa 48’, ‘BRS 132’. ‘BRS 183’, ‘BRS 212’, ‘IAC 22’, and ‘IAC 23’ (early cycled varieties), ‘BRS 133’, ‘BRS 154’, ‘BRS 156’, ‘BRS 184’, ‘BRS 214’, ‘IAC 18’, and ‘IAC 24’ ( semi early varieties), and ‘BRS 134’, ‘BRS 215’, ‘IAC 8.2’, and ‘IAC 19’ (medium cycled varieties ). All the varieties, during the three cropping years, showed adequate plant height and first pod height of insertion for mechanical harvest. Among the production components, mass of 100 grains showed the highest variability. Cultivar ‘BRS 154’ (medium cycle) showed the highest variation in mass of 100 grains and was also the highest yielding variety in the cropping year of 2004/05. The majority of the cultivars yielded above 3,000 kg ha -1 during the cropping years of 2002/03 and 2004/05. The best yielding performance during the three cropping years were displayed by cultivars ‘IAC 22’ (early cycle), ‘BRS 133’ and ‘BRS 156’ ( both semi early cycled varieties).

Starch accumulation in cassava roots: spatial and temporal distribution

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Performance of different rubber tree clones against South American leaf blight (Microcyclus ulei)

This study was aimed to evaluate the behaviour of eighteen clones of Hevea brasiliensis (rubber) against South American leaf blight and tostudy progress of the disease. The experiment was conducted in Cachoeiro de Itapemirim-ES, where 18 clones were evaluated. The experi-mental design was a randomized block with four replicates; each experimental unit consisted of three plants. Evaluations were performed at15-day intervals on 30 leaflets per tree. Disease incidence was quantified and infection classified according to the stages of development andtype of damage. Leaf blight occurred during the entire experimental period; however, disease intensity varied with the resistance level of theclones and the time of year. Clones FX 3864, RRIM 725, RRIM 711, IAC 300 and IAN 873 exhibited the highest resistance to leaf blight.

Uso de estimulantes de crescimento radicular associado a doses de fósforo na cultura do feijoeiro

O trabalho teve como objetivo avaliar o efeito da aplicação do Stimulate e Forth Seed associados a cinco doses de fósforo(P) no desenvolvimento do sistema radicular e parte aérea de plantas de feijão. O experimento foi desenvolvido em condições de casa de vegetação nas dependências do Departamento de Recursos Naturais – Ciência do Solo, da Faculdade de Ciências Agronômicas (FCA), UNESP, Campus de Botucatu, SP. O solo usado foi um Latossolo Vermelho Escuro de textura média. As unidades experimentais foram vasos contendo 8 dm3 de solo mantendo-se a umidade correspondente a 60% do Volume Total de Poros (VTP) ocupados por água. Utilizou-se a cultivar de feijão IAC-Carioca, em um delineamento experimental inteiramente casualizado, sendo os tratamentos constituídos de dois produtos (Stimulate e Forth Seed) nas respectivas doses 5 e 3 mL Kg-1 de sementes e uma testemunha sem biofertilizante, e 5 doses de P (0, 16, 32, 64 e 128 mg dm-3), com três repetições. Foi avaliada, também, a qualidade fisiológica das sementes usadas no experimento por meio de: a) primeira contagem do teste padrão de germinação; b) emergência; c) vigor, mediante a contagem de emergência; d) velocidade de emergência e determinação do crescimento das plântulas. O uso de Stimulate e Forth Seed agregados a doses de P causou redução no peso seco final das plantas.

Avaliação de atributos físico-químicos do solo sobre palhadas de diferentes poáceas e adubação nitrogenada em semeadura direta na cultura do feijoeiro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Mapping of angular leaf spot resistance QTL in common bean (Phaseolus vulgaris L.) under different environments

Background: Common bean (Phaseolus vulgaris L.) is the most important grain legume for human diet worldwide and the angular leaf spot (ALS) is one of the most devastating diseases of this crop, leading to yield losses as high as 80%. In an attempt to breed resistant cultivars, it is important to first understand the inheritance mode of resistance and to develop tools that could be used in assisted breeding. Therefore, the aim of this study was to identify quantitative trait loci (QTL) controlling resistance to ALS under natural infection conditions in the field and under inoculated conditions in the greenhouse. Results: QTL analyses were made using phenotypic data from 346 recombinant inbreed lines from the IAC-UNA x CAL 143 cross, gathered in three experiments, two of which were conducted in the field in different seasons and one in the greenhouse. Joint composite interval mapping analysis of QTL x environment interaction was performed. In all, seven QTLs were mapped on five linkage groups. Most of them, with the exception of two, were significant in all experiments. Among these, ALS10.1(DG,UC) presented major effects (R-2 between 16% - 22%). This QTL was found linked to the GATS11b marker of linkage group B10, which was consistently amplified across a set of common bean lines and was associated with the resistance. Four new QTLs were identified. Between them the ALS5.2 showed an important effect (9.4%) under inoculated conditions in the greenhouse. ALS4.2 was another major QTL, under natural infection in the field, explaining 10.8% of the variability for resistance reaction. The other QTLs showed minor effects on resistance. Conclusions: The results indicated a quantitative inheritance pattern of ALS resistance in the common bean line CAL 143. QTL x environment interactions were observed. Moreover, the major QTL identified on linkage group B10 could be important for bean breeding, as it was stable in all the environments. Thereby, the GATS11b marker is a potential tool for marker assisted selection for ALS resistance.

ASSESSING Bemisia tabaci (GENN.) BIOTYPE B RESISTANCE IN SOYBEAN GENOTYPES: ANTIXENOSIS AND ANTIBIOSIS

Since it was first reported in Brazil in the 1990s, the B biotype of silverleaf whitefly (Bemisia tabaci [Genn.], Hemiptera: Aleyrodidae) has been recognized as an important pest in soybeans (Glycine max L.), reducing the productivity of this legume species in some areas of the country. As an alternative to chemical control, the use of resistant genotypes represents an important tool for integrated pest management (IPM). This study evaluated the performance of 10 soybean genotypes prior to whitefly infestation, by testing attractiveness and preference for oviposition in the greenhouse and antibiosis in the laboratory. In a multiple-choice test, 'IAC-17' was the least attractive to insects. In a no-choice test, 'IAC-17' was the least attractive for, egg deposition, indicating the occurrence of non-preference for oviposition on this genotype. Trichome density was positively correlated with the oviposition site and may be associated with the resistance of 'IAC-17' to infestation. The genotypes 'IAC-PL1', 'IAC-19', 'Conquista', 'IAC-24' and 'IAC-17' extended the insect's life cycle, indicating the occurrence of a small degree of antibiosis and/or non-preference for feeding.

Insulin alone or with captopril: effects on signaling pathways (AKT and AMPK) and oxidative balance after ischemia-reperfusion in isolated hearts

Insulin and the inhibition of the reninangiotensin system have independent benefits for ischemiareperfusion injury, but their combination has not been tested. Our aim was to evaluate the effects of insulin+captopril on insulin/angiotensin signaling pathways and cardiac function in the isolated heart subjected to ischemiareperfusion. Isolated hearts were perfused (Langendorff technique) with KrebsHenseleit (KH) buffer for 25 min. Global ischemia was induced (20 min), followed by reperfusion (30 min) with KH (group KH), KH+angiotensin-I (group A), KH+angiotensin-I+captopril (group AC), KH+insulin (group I), KH+insulin+angiotensin-I (group IA), or KH+insulin+angiotensin-I+captopril (group IAC). Group A had a 24% reduction in developed pressure and an increase in end-diastolic pressure vs. baseline, effects that were reverted in groups AC, IA, and IAC. The phosphorylation of protein kinase B (AKT) was higher in groups I and IA vs. groups KH and A. The phosphorylation of AMP-activated protein kinase (AMPK) was similar to 31% higher in groups I, IA, and IAC vs. groups KH, A, and AC. The tert-butyl hydroperoxide (tBOOH)-induced chemiluminescence was lower (similar to 2.2 times) in all groups vs. group KH and was similar to 35% lower in group IA vs. group A. Superoxide dismutase content was lower in groups A, AC, and IAC vs. group KH. Catalase activity was similar to 28% lower in all groups (except group IA) vs. group KH. During reperfusion of the ischemic heart, insulin activates the AKT and AMPK pathways and inhibits the deleterious effects of angiotensin-I perfusion on SOD expression and cardiac function. The addition of captopril does not potentiate these effects.