Brevetoxin in two planktivorous fishes after exposure to Karenia brevis: implications for food-web transfer to bottlenose dolphins

Brevetoxin uptake was analyzed in 2 common planktivorous fish that are likely foodweb vectors for dolphin mortality events associated with brevetoxin-producing red tides. Fish were exposed to brevetoxin-producing Karenia brevis for 10 h under conditions previously reported to produce optimal uptake of toxin in blood after oral exposure. Striped mullet Mugil cephalus were exposed to a low dose of brevetoxin, and uptake and depuration by specific organs were evaluated over a 2 mo period. Atlantic menhaden Brevoortia tyrannus specimens were used to characterize a higher brevetoxin dose uptake into whole body components and evaluate depuration over 1 mo. We found a high uptake of toxin by menhaden, with a body to water ratio of 57 after a 10 h exposure and a slow elimination with a half life (t1/2) of 24 d. Elimination occurred rapidly from the intestine (t1/2 < 1 wk) and muscle (t1/2 ≈ 1 wk) compartments and redistributed to liver which continued to accumulate body stores of toxin for 4 wk. The accumulation and elimination characteristics of the vectoring capacity of these 2 fish species are interpreted in relation to data from the Florida Panhandle dolphin mortality event of 2004. We show that due to slow elimination rate of brevetoxin in planktivorous fish, brevetoxin-related dolphin mortality events may occur without evidence of a concurrent harmful algal bloom event.

Swimming depth, ambient water temperature preference, and feeding frequency of young Pacific bluefin tuna (Thunnus orientalis) determined with archival tags

We investigated the migration and behavior of young Pacific Bluefin tuna (Thunnus orientalis) using archival tags. The archival tag measures environmental variables, records them in its memory, and estimates daily geographical locations based on measured light levels. Of 166 archival tags implanted in Pacific bluefin tuna that were released at the northeastern end of the East China Sea from 1995 to 1997, 30 tags were recovered, including one from a fish that migrated across the Pacific. This article describes swimming depth, ambient water temperature, and feeding frequency of young Pacific bluefin tuna based on retrieved data. Tag performance, effect of the tag on the fish, and horizontal movements of the species are described in another paper. Young Pacific bluefin tuna swim mainly in the mixed layer, usually near the sea surface, and swim in deeper water in daytime than at nighttime. They also exhibit a pattern of depth changes, corresponding to sunrise and sunset, apparently to avoid a specific low light level. The archival tags recorded temperature changes in viscera that appear to be caused by feeding, and those changes indicate that young Pacific bluefin tuna commonly feed at dawn and in the daytime, but rarely at dusk or at night. Water temperature restricts their distribution, as indicated by changes in their vertical distribution with the seasonal change in depth of the thermocline and by the fact that their horizontal distribution is in most cases confined to water in the temperature range of 14−20°C.

Community water access, availability and management in the Tonle Sap region, Cambodia.

During the rollout of CGIAR Research Program on Aquatic Agricultural Systems (AAS) in Tonle Sap in 2013, water management was highlighted as one of the key development challenges. With limited capacity to regulate water, the situation oscillates between too much water in the wet season and too little water in the dry season. Access to and availability of water were seen by local communities as major limitations for aquatic and agricultural production, impacting on functions that include the lake fishery, intensive (dry season) rice crops, recession rice, rainfed rice and floating rice by the lakeside. For both fish and rice production, water and water management are determined principally by the natural flooding of the Tonle Sap Lake. This study is based on a community survey on water access, availability and management and was conceived out of the AAS consultation process and was developed to help identify existing practices in water use and management, as well as best practices where lessons can be learned and promising activities scaled out to other communities. The community survey also aims to understand, identify and analyze constraints and opportunities related to water, and includes a gender perspective to better understand the role of women in water management and use.

Studies on morphometry and hydrology of Gandhisagar Reservoir with special reference to its fisheries

Gandhisagar, the second largest reservoir of India is located in Mandsaur district of Madhya Pradesh at latitude 24°44'N and longitude 75°33'E at an altitude of 403.56m MSL in orientation from NE to SE. It has an extensive water spread area of 66000 ha at full reservoir level with a maximum and mean depth of 49.52 and 11.73 m respectively. The maximum length and width of the reservoir are 112 and 16km having a total shore line of 442km. Details of catchment area, bathymetry, standard hydrological data giving water level relation of the basin to water spread area, volume and fish production and the bottom topographical details of 11 experimental fishing stations and 6 fish landing centres are discussed.

Human-humanoid interaction: Is a humanoid robot perceived as a human?

As humanoid robots become more commonplace in our society, it is important to understand the relation between humans and humanoid robots. In human face-to-face interaction, the observation of another individual performing an action facilitates the execution of a similar action, and interferes with the execution of different action. This phenomenon has been explained by the existence of shared internal representations for the execution and perception of actions, which would be automatically activated by the perception of another individual's action. In one interference experiment, null interference was reported when subjects observed a robotic arm perform the incongruent task, suggesting that this effect may be specific to interacting with other humans. This experimental paradigm, designed to investigate motor interference in human interactions, was adapted to investigate how similar the implicit perception of a humanoid robot is to a human agent. Subjects performed rhythmic arm movements while observing either a human agent or humanoid robot performing either congruent or incongruent movements. The variance of the executed movements was used as a measure of the amount of interference in the movements. Both the human and humanoid agents produced significant interference effect. These results suggest that observing the action of humanoid robot and human agent may rely on similar perceptual processes. Furthermore, the ratio of the variance in incongruent to congruent conditions varied between the human agent and humanoid robot. We speculate this ratio describes how the implicit perception of a robot is similar to that of a human, so that this paradigm could provide an objective measure of the reaction to different types of robots and be used to guide the design of humanoid robots interacting with humans. © 2004 IEEE.

Interactions of dietary levels of protein and energy on rainbow trout (Oncorhynchus mykiss) in desert brackish water

A 3x3 factorial experiment was conducted to determine the optimum protein to energy (P/E) ratio for rainbow trout in brackish water. Three crud protein levels and three energy levels at each protein level were utilized. Diets were made in semi-purified that in all of them fish meal, casein and gelatin as the sources of protein and dextrin, starch and oil as the sources of energy were used. Each of experimental diets was fed to triplicate groups of 20 fish with an average individual weight of 81.5 g in 9 2000-1 flow trough fiberglass tanks. During this experiment water temperature, dissolved oxygen, PH and EC were 15±2°C, 6.5-8.1 mg/1, 7.7-8.6 and 25400 grills respectively. The diets were fed at a rate between 1.6-2 wet body weight% per day depended to water temperature in three equal rations and adjusted two weekly for 84 days. At each of protein levels, weight gain percent (%WG), average daily growth percent (%ADG), protein efficiency ratio (PER), apparent net protein utilization percent (%ANPU), or percent of protein deposited, specific growth rate (SGR) and condition factor (CF) were found to increase and food conversion ratio (FCR) was found to decrease with an increasing energy levels from 370 to 430 Kcal/100g. Fish fed a 35% protein, 430 Kcal/100g energy diet with a P/E ratio of 81.4 mg protein/ Kcal PFV energy, attained the best growth performance. Fat and moisture of carcass were affected by protein and energy levels of test diets while protein and ash of carcass were relatively constant in different treatments.

Habitat use by wintering Ruddy Shelduck at Lashihai Lake, Lijiang, China

Habitat use by wintering Ruddy Shelduck (Tadorna ferruginea) in Lijiang Lashihai Lake of southwest China was studied from 1 November 1999 to 29 April 2000. We divided habitats into five types-deep water, shallow water, mudflat, grassland and farmland. Shallow water and grassland, with rich food and easily accessible water, were preferred by wintering Ruddy Shelducks, Farmland was preferred in mid-winter but avoided in early winter and late winter. Even in mid-winter, the feeding Ruddy Shelduck on farmland were not equally distributed in fields and preferred wet fields (just irrigated) and avoided dry fields. In dry fields, the distances to water sources had great impact on the feeding distribution. Mudflats were only selected in later winter, coinciding with the growth of water-weeds. Deep-water areas were always avoided. Prohibition of human disturbance and retaining shallow water areas and grassland are important measures to mitigate conflict between Ruddy Sheldruck and local people.

Geographic and economic setting of Lake Victoria

Lake Victoria, besides being the second largest in the world after Lake Superior, is the largest tropical lake. Its waters are shared by Kenya (6% of the surface area), Uganda (43%), and Tanzania (51%). Before dramatic structural and functional changes manifested in the lake's ecosystem especially in the 1980s, fish life flourished in the lake's entire water column at all times of the year. Currently, the situation is much more different from what it was in the past. The exponential increase in the introduced Nile perch (Lates niloticus) and Nile tilapia (Oreochromis niloticus) stocks, siltation, wetland degradation and eutrophication have characterised the lake ecosystem. The two exotic species and the small native cyprinid (Rastrineobola argentea) form the basis of the commercial fishery that was once dominated by two native tilapiines (Oreochromis esculentus and Oreochromis variabilis) and five other large-bodied endemic fishes. Severe deoxygenation observed at shallow depths (Ochumba 1990; Hecky et al., 1994) indicates that a large volume of the lake is unable to sustain fish life. The Lake Victoria catchment is one of the most densely populated areas in East Africa, encompassing a population of about 30 million people. Widespread poverty resulting from high inflation rates, lack of opportunities and general unemployment have characterised the lakeside communities over much of the last two decades. The biophysical environment in which Lake Victoria exists makes the lake particularly susceptible to changes that occur as a result of human modification to the watershed or the lake itself, thus rendering benefits from the lake unsustainable.

Pacific Islands: Next to slavery

Workers and crew on board vessels in the Pacific tuna fishery rarely enjoy the right to decent living and working conditions...

Capture fisheries in Uganda: why Bujagali hydro-power project and ecological monitoring?

Human use of water resow-ces in Uganda has grown and intensified along with population growth and increasing demand to meet the diverse human needs. In the case of Uganda's rivers, the main uses include fisheries, hydropower generation, abstraction for potable water supply, discharge of sewage and navigation. All these uses can disrupt the integrity of the aquatic ecosystem and may affect the survival of the diversity of organisms. In consideration of the need to increase electricity to meet demand, the Bujagali Hydro-power Project (BHPP) and the National Environment Management Authority (NEMA) recognised the importance of safeguards to mitigate impacts of the project. The National Fisheries Resources Research Institute (NaFIRRI) was assigned the role of providing baseline information on the aquatic ecosystem of the Upper Victoria Nile and to follow up the findings with a monitoring framework during construction and post-commissioning phases.

Cell adhesion to plasma electrolytic oxidation (PEO) titania coatings, assessed using a centrifuging technique.

The adhesion of bovine chondrocytes and human osteoblasts to three titania-based coatings, formed by plasma electrolytic oxidation (PEO), was compared to that on uncoated Ti-6Al-4V substrates, and some comparisons were also made with plasma sprayed hydroxyapatite (HA) coatings. This was done using a centrifuge, with accelerations of up to 160,000 g, so as to induce buoyancy forces that created normal or shear stresses at the interface. It is shown that, on all surfaces, it was easier to remove cells under normal loading than under shear loading. Cell adhesion to the PEO coatings was stronger than that on Ti-6Al-4V and similar to that on HA. Cell proliferation rates were relatively high on one of the PEO coatings, which was virtually free of aluminium, but low on the other two, which contained significant levels of aluminium. It is concluded that the Al-free PEO coating offers promise for application to prosthetic implants.

Environmental factors influencing composition, abundance and distribution of water hyacinth

Relationships between nutrient concentrations and water hyacinth biomass and composition have been studied in the shallow inshore bays of lakes Victoria, Kyoga and Albert. Additional information was obtained from Victoria Nile, Albert Nile and Kagera River. In this section, seasonal changes in nutrients and oxygen concentrations are used to explain changes in water hyacinth composition, biomass and distribution in Lake Victoria. Lake Victoria is of particular interest because it experienced strong hyacinth infestations in 1995, a sink in 1998 and resurgence in 2001. The lake has also been extensively sampled and provides time series data in nutrient, oxygen, mixing and thermal stratification which provide an opportunity to relate water hyacinth distribution and biomass to environmental factors. The possible origins and impacts of nutrient loads into Lake Victoria are also discussed in relation to water hyacinth proliferation and distribution especially in relation to known 'hot-spots'.

A vertically aligned carbon nanotube/fiber based electrode for economic hydrogen production by water electrolysis

Electrolysis is the most mature form of hydrogen production. Unfortunately, water electrolysis has not yet achieved the efficiency and the cost levels required for any practical application. In order to enhance the current density, modification of the electrolyte and the electrode morphology are the most popular approaches. Recently there have been numerous reports on how to improve the efficiency of hydrogen production by water splitting [1-3]. On the electrode side, the use of non-platinum high efficiency electrode materials for water splitting will provide a promising future for the hydrogen economy. An ideal electrode for water electrolysis should have good permeability to water and gas. It should also offer good electrical properties with a long life. A porous graphite plate, when coated with titania, for example, is known to provide a simple and economical electrode for water electrolysis [4]. © 2010 IEEE.

Water, salt water, and alkaline solution uptake in epoxy thin films

As a means of characterizing the diffusion parameters of fiber reinforced polymer (FRP) composites within a relatively short time frame, the potential use of short term tests on epoxy films to predict the long-term behavior is investigated. Reference is made to the literature to assess the effectiveness of Fickian and anomalous diffusion models to describe solution uptake in epoxies. The influence of differing exposure conditions on the diffusion in epoxies, in particular the effect of solution type and temperature, are explored. Experimental results, where the solution uptake in desiccated (D) or undesiccated (U) thin films of a commercially available epoxy matrix subjected to water (W), salt water (SW), or alkali concrete pore solution (CPS) at either 20 or 60°C, are also presented. It was found that the type of solution did not significantly influence the diffusion behavior at 20°C and that the mass uptake profile was anomalous. Exposure to 60°C accelerated the initial diffusion behavior and appeared to raise the level of saturation. In spite of the accelerated approach, conclusive values of uptake at saturation remained elusive even at an exposure period of 5 years. This finding questions the viability of using short-term thin film results to predict the long-term mechanical performance of FRP materials. © 2013 Wiley Periodicals, Inc.

PCR-DGGE Fingerprinting Analysis of Plankton Communities and Its Relationship to Lake Trophic Status

Plankton communities in eight lakes of different trophic status near Yangtze, China were characterized by using denatured gradient gel electrophoresis (DGGE). Various water quality parameters were also measured at each collection site. Following extraction of DNA from plankton communities, 16S rRNA and 18S rRNA genes were amplified with specific primers for prokaryotes and eukaryotes, respectively; DNA profiles were developed by DGGE. The plankton community of each lake had its own distinct DNA profile. The total number of bands identified at 34 sampling stations ranged from 37 to 111. Both prokaryotes and eukaryotes displayed complex fingerprints composed of a large number of bands: 16 to 59 bands were obtained with the prokaryotic primer set; 21 to 52 bands for the eukaryotic primer set. The DGGE-patterns were analyzed in relation to water quality parameters by canonical correspondence analysis (CCA). Temperature, pH, alkalinity, and the concentration of COD, TP and TN were strongly correlated with the DGGE patterns. The parameters that demonstrated a strong correlation to the DGGE fingerprints of the plankton community differed among lakes, suggesting that differences in the DGGE fingerprints were due mainly to lake trophic status. Results of the present study suggest that PCR-DGGE fingerprinting is an effective and precise method of identifying changes to plankton community composition, and therefore could be a useful ecological tool for monitoring the response of aquatic ecosystems to environmental perturbations.