PTPN22 Association in Systemic Lupus Erythematosus (SLE) with Respect to Individual Ancestry and Clinical Sub-Phenotypes

Protein tyrosine phosphatase non-receptor type 22 (PTPN22) is a negative regulator of T-cell activation associated with several autoimmune diseases, including systemic lupus erythematosus (SLE). Missense rs2476601 is associated with SLE in individuals with European ancestry. Since the rs2476601 risk allele frequency differs dramatically across ethnicities, we assessed robustness of PTPN22 association with SLE and its clinical subphenotypes across four ethnically diverse populations. Ten SNPs were genotyped in 8220 SLE cases and 7369 controls from in European-Americans (EA), African-Americans (AA), Asians (AS), and Hispanics (HS). We performed imputation-based association followed by conditional analysis to identify independent associations. Significantly associated SNPs were tested for association with SLE clinical sub-phenotypes, including autoantibody profiles. Multiple testing was accounted for by using false discovery rate. We successfully imputed and tested allelic association for 107 SNPs within the PTPN22 region and detected evidence of ethnic-specific associations from EA and HS. In EA, the strongest association was at rs2476601 (P = 4.761029, OR = 1.40 (95% CI = 1.25–1.56)). Independent association with rs1217414 was also observed in EA, and both SNPs are correlated with increased European ancestry. For HS imputed intronic SNP, rs3765598, predicted to be a cis-eQTL, was associated (P = 0.007, OR = 0.79 and 95% CI = 0.67–0.94). No significant associations were observed in AA or AS. Case-only analysis using lupus-related clinical criteria revealed differences between EA SLE patients positive for moderate to high titers of IgG anti-cardiolipin (aCL IgG .20) versus negative aCL IgG at rs2476601 (P = 0.012, OR = 1.65). Association was reinforced when these cases were compared to controls (P = 2.761025, OR = 2.11). Our results validate that rs2476601 is the most significantly associated SNP in individuals with European ancestry. Additionally, rs1217414 and rs3765598 may be associated with SLE. Further studies are required to confirm the involvement of rs2476601 with aCL IgG.

Ley de libre determinación moderna y la 'cuarta opción': Ley internacional y de Estados Unidos

Conforme a las resoluciones de la ONU 1514 y 1541 (1960), usualmente se discuten solo tres formas de autodeterminación de los pueblos: la independencia, la integración y la libre asociación. En 1970, la Resolución 2625 amplió ese lenguaje, añadiendo una cuarta vía: “La adquisición de cualquier otra condición política libremente decidida por un pueblo”. La Resolución 2625 y resoluciones posteriores plantean un marco de libertad de petición y negociación que no limita la búsqueda de soluciones razonables y realistas a las aspiraciones legítimas de progreso político, cultural y económico de un pueblo.La monografía también discute estas normas desde la perspectiva del Derecho Constitucional de los Estados Unidos, concluyendo que ese corpus jurisprudencial también reconoce y permite diversas formas de organización política “soberana” más allá de los estados de la Unión, pueblos o jurisdicciones con diversos grados de autonomía, desde las tribus o naciones indias, hasta los Estados libres asociados, consistentes con el espíritu y letra de la Resolución 2625 y su progenie.

Guayaquil: un puerto colonial en los mares del sur, siglo XVIII (Estudios)

Este artículo analiza el emplazamiento urbano, las dinámicas económicas y las relaciones sociales que caracterizaron a Guayaquil durante el XVIII. El desarrollo económico y productivo que experimentó la ciudad-puerto se debió a su feraz entorno natural y a las posibilidades de comunicación que brindó el sistema fluvial del Guayas. Se analizan las particularidades de su estructura social, las relaciones entre la élite y los sectores subalternos, la estructura político-administrativa del Cabildo y la influencia que este centro urbano ejerció sobre los pueblos y partidos ubicados en su hinterland.

Generation of candidate human influenza vaccine strains in cell culture: rehearsing the European response to an H7N1 pandemic threat

Background: Although H5N1 avian influenza viruses pose the most obvious imminent pandemic threat, there have been several recent zoonotic incidents involving transmission of H7 viruses to humans. Vaccines are the primary public health defense against pandemics, but reliance on embryonated chickens eggs to propagate vaccine and logistic problems posed by the use of new technology may slow our ability to respond rapidly in a pandemic situation. Objectives: We sought to generate an H7 candidate vaccine virus suitable for administration to humans whose generation and amplification avoided the use of eggs. Methods: We generated a suitable H7 vaccine virus by reverse genetics. This virus, known as RD3, comprises the internal genes of A/Puerto Rico/8/34 with surface antigens of the highly pathogenic avian strain A/Chicken/Italy/13474/99 (H7N1). The multi-basic amino acid site in the HA gene, associated with high pathogenicity in chickens, was removed. Results: The HA modification did not alter the antigenicity of the virus and the resultant single basic motif was stably retained following several passages in Vero and PER. C6 cells. RD3 was attenuated for growth in embryonated eggs, chickens, and ferrets. RD3 induced an antibody response in infected animals reactive against both the homologous virus and other H7 influenza viruses associated with recent infection by H7 viruses in humans. Conclusions: This is the first report of a candidate H7 vaccine virus for use in humans generated by reverse genetics and propagated entirely in mammalian tissue culture. The vaccine has potential use against a wide range of H7 strains.

A new genus and new species of fan worms (Polychaeta: Sabellidae) from Atlantic and Pacific Oceans-the formal treatment of taxon names as explanatory hypotheses

Members of Parasabella minuta Treadwell, 1941, subsequently moved to Perkinsiana, were collected during a survey of rocky intertidal polychaetes along the state of Sao Paulo, Brazil. Additional specimens, which are referred to two new species, were also found in similar habitats from the Bocas del Toro Archipelago, Caribbean Panama, and Oahu Island, Hawaii. A phylogenetic analysis of Sabellinae, including members of P. minuta and the two new species, provided justification for establishing a new generic hypothesis, Sabellomma gen. nov., for these individuals. Formal definitions are also provided for Sabellomma minuta gen. nov., comb. nov., S. collinae gen. nov., spec. nov., and S. harrisae gen. nov., spec. nov., along with descriptions of individuals to which these hypotheses apply. The generic name Aracia nom. nov., is provided to replace Kirkia Nogueira, Lopez and Rossi, 2004, pre-occupied by a mollusk.

Coptotermes gestroi (Isoptera: Rhinotermitidae) in Brazil: possible origins inferred by mitochondrial cytochrome oxidase II gene sequences

The Asian subterranean termite, Coptotermes gestroi, originally from northeast India through Burma, Thailand, Malaysia, and the Indonesian archipelago, is a major termite pest introduced in several countries around the world, including Brazil. We sequenced the mitochondrial COII gene from individuals representing 23 populations. Phylogenetic analysis of COII gene sequences from this and other studies resulted in two main groups: (1) populations of Cleveland (USA) and four populations of Malaysia and (2) populations of Brazil, four populations of Malaysia, and one population from each of Thailand, Puerto Rico, and Key West (USA). Three new localities are reported here, considerably enlarging the distribution of C. gestroi in Brazil: Campo Grande (state of Mato Grosso do Sul), Itajai (state of Santa Catarina), and Porto Alegre (state of Rio Grande do Sul).

International migration, human rights and development in Latin America and the Caribbean: summary and conclusions

Includes bibliography

Trade and investment flows between the Caribbean and the rest of the hemisphere in the context of the FTAA

Includes bibliography

Tourism life cycle, tourism competitiveness and upgrading strategies in the Caribbean

In the 1980s Butler adapted the life cycle product model to the tourism industry and created the “Tourism Area Life Cycle (TALC) model”. The model recognizes six stages in the tourism product life cycle: exploration, investment, development, consolidation, stagnation and followed, after stagnation, by decline or revitalization of the product. These six stages can in turn be regrouped into four main stages. The Butler model has been applied to more than 30 country cases with a wide degree of success. De Albuquerque and Mc Elroy (1992) applied the TALC model to 23 small Caribbean island States in the 1990s. Following De Albuquerque and Mc Elroy, the TALC is applied to the 32 member countries of the Caribbean Tourism Organization (CTO) (except for Cancun and Cozumel) to locate their positions along their tourism life-cycle in 2007. This is done using the following indicators: the evolution of the level, market share and growth rate of stay-over arrivals; the growth rate and market share of visitor expenditures per arrival and the tourism styles of the destinations, differentiating between ongoing mass tourism and niche marketing strategies and among upscale, mid-scale and low-scale destinations. Countries have pursued three broad classes of strategies over the last 15 years in order to move upward in their tourism life cycle and enhance their tourism competitiveness. There is first a strategy that continues to rely on mass-tourism to build on the comparative advantages of “sun, sand and sea”, scale economies, all-inclusive packages and large amounts of investment to move along in Stage 2 or Stage 3 (Cuba, Dominican Republic, Puerto Rico). There is a second strategy pursued mainly by very small islands that relies on developing specific niche markets to maintain tourism competitiveness through upgrading (Anguilla, Antigua and Barbuda, British Virgin Islands and Turks and Caicos), allowing them to move from Stage 2 to Stage 3 or Stage 3 to a rejuvenation stage. There is a third strategy that uses a mix of mass-tourism, niche marketing and quality upgrading either to emerge onto the intermediate stage (Trinidad and Tobago); avoid decline (Aruba, The Bahamas) or rejuvenate (Barbados, Jamaica and the United States Virgin Islands). There have been many success stories in Caribbean tourism competitiveness and further research should aim at empirically testing the determinants of tourism competitiveness for the region as a whole.

Implementation of the 2014-2015 programme of work: subprogramme 13: subregional activities in the Caribbean

This report highlights the activities carried out by ECLAC in the Caribbean subregion between 1 January 2014 and 31 March 2015. Subprogramme 13 of the ECLAC programme of work 2014-2015 (“Subregional activities in the Caribbean”) covers the Commission’s work in Antigua and Barbuda, the Bahamas, Barbados, Belize, Cuba, Dominica, the Dominican Republic, Grenada, Guyana, Haiti, Jamaica, Saint Kitts and Nevis, Saint Lucia, Saint Vincent and the Grenadines, Suriname and Trinidad and Tobago, as well as Anguilla, Aruba, the British Virgin Islands, the Cayman Islands, Curaçao, Guadeloupe, Martinique, Montserrat, Puerto Rico, Sint Maarten, the Turks and Caicos Islands and the United States Virgin Islands. Subprogramme 12 (“Subregional activities in Central America, Cuba, the Dominican Republic, Haiti and Mexico”) includes activities conducted in the Caribbean member States of Cuba, the Dominican Republic and Haiti. In addition, countries of the Caribbean were included in activities organized under the 12 other substantive subprogrammes of the ECLAC programme of work 2014-2015, namely: (i) linkages with the global economy, integration and regional cooperation; (ii) production and innovation; (iii) macroeconomic policies and growth; (iv) financing for development; (v) social development and equality; (vi) mainstreaming the gender perspective in regional development; (vii) population and development; (viii) sustainable development and human settlements; (ix) natural resources and infrastructure; (x) planning of public administration; (xi) statistics; and (xii) support for regional and subregional integration and cooperation processes and organizations.

Uso da L-arginina nos processos de capacitação espermática e fecundação in vitro de oócitos bovinos

O objetivo deste trabalho foi avaliar o uso da L-arginina nos processos de capacitação espermática e fecundação in vitro (FIV), analisando sua influência no desenvolvimento embrionário, utilizando o sêmen de dois touros (Bos taurus e Bos indicus). No experimento 1, os espermatozóides foram incubados, sem a presença de oócitos, durante 0, 1, 2 e 3 h em meio de FIV adicionado de 0, 1, 10 e 50 mM de L-arginina, sendo analisada a taxa de reação acrossômica. No experimento 2, espermatozóides e oócitos foram incubados em meio de FIV acrescido com as concentrações de L-arginina citadas anteriormente, durante aproximadamente 30 h. Os oócitos bovinos foram maturados in vitro (MIV) e o subsequente cultivo embrionário (CIV) foi realizado sobre monocamada de células da granulosa, em meio SOF, sendo avaliadas as taxas de fecundação (18 hpi), clivagem e blastocisto (2º e 7º dia de cultivo, respectivamente). A dosagem de NO₃ ^-/NO₂ ^- produzido durante a FIV foi realizada através do método colorimétrico de Griess. Para análise estatística dos dados, foi utilizado a ANOVA, com nível de significância de 5%. A Larginina (1 mM), quando adicionada ao meio de capacitação espermática, durante duas horas, aumentou a taxa de reação acrossômica em relação ao controle (31,1±2,78 vs 23,4±2,65) em Bos taurus. A adição de L-arginina (50 mM) ao meio de FIV (experimento 2), tanto em Bos taurus quanto em Bos indicus, diminuiu as taxas de clivagem (78,7±2,17 vs 65,7±9,32; 72,7±3,36 vs 45±7,12; respectivamente) e blastocisto (39,4±3,78 vs 15,2±6,12; 39,4±4,39 vs 16±8,54; respectivamente) em relação ao controle. Sendo assim, observou-se que a L-arginina aumentou a taxa de reação acrossômica em Bos taurus, porém reduziu as taxas de clivagem e blastocistos em ambos os touros, sem influenciar na qualidade do embrião.

Health related quality of life measure in systemic pediatric rheumatic diseases and its translation to different languages: an international collaboration

Background: Rheumatic diseases in children are associated with significant morbidity and poor health-related quality of life (HRQOL). There is no health-related quality of life (HRQOL) scale available specifically for children with less common rheumatic diseases. These diseases share several features with systemic lupus erythematosus (SLE) such as their chronic episodic nature, multi-systemic involvement, and the need for immunosuppressive medications. HRQOL scale developed for pediatric SLE will likely be applicable to children with systemic inflammatory diseases.Findings: We adapted Simple Measure of Impact of Lupus Erythematosus in Youngsters (SMILEY (c)) to Simple Measure of Impact of Illness in Youngsters (SMILY (c)-Illness) and had it reviewed by pediatric rheumatologists for its appropriateness and cultural suitability. We tested SMILY (c)-Illness in patients with inflammatory rheumatic diseases and then translated it into 28 languages. Nineteen children (79% female, n= 15) and 17 parents participated. The mean age was 12 +/- 4 years, with median disease duration of 21 months (1-172 months). We translated SMILY (c)-Illness into the following 28 languages: Danish, Dutch, French (France), English (UK), German (Germany), German (Austria), German (Switzerland), Hebrew, Italian, Portuguese (Brazil), Slovene, Spanish (USA and Puerto Rico), Spanish (Spain), Spanish (Argentina), Spanish (Mexico), Spanish (Venezuela), Turkish, Afrikaans, Arabic (Saudi Arabia), Arabic (Egypt), Czech, Greek, Hindi, Hungarian, Japanese, Romanian, Serbian and Xhosa.Conclusion: SMILY (c)-Illness is a brief, easy to administer and score HRQOL scale for children with systemic rheumatic diseases. It is suitable for use across different age groups and literacy levels. SMILY (c)-Illness with its available translations may be used as useful adjuncts to clinical practice and research.

RB30-252 Sex and Age as Factors in Cattle Feeding

Varying economic conditions and changes in the demands of the meat consuming public have been responsible for the turns that have taken place in the beef industry during recent years. Both feeder and producer must recognize and conform to these changes if they are to continue in business. Among the most important of these changes have been the turn toward the marketing of lighter cattle and the gradual disappearance from feed lots of two- and three-year-old animals. Furthermore, the cattle population of the United States is fast reaching stabilization with the resulting effect that more heifers are being marketed, since only one-fourth of the heifer crop is needed to replace worn-out breeding animals. Realizing the increasing importance of the heifer problem from the standpoint of the producer, feeder, and consumer, the Nebraska Experiment Station undertook to compare steers and heifers in a series of trials both in the feedlot and in the beef. It was hoped that these experiments would yield results which would bring out existing differences, if any, between steers and heifers both in quality and quantity of beef produced and thus provide or disprove many of the complaints against heifers. The results of these trials are summarized in this bulletin. Age as well as the sex factor has been considered, since two-year-olds, yearlings, and calves were included in these trials.

Examination of Homalometron elongatum Manter, 1947 and Description of a New Congener from Eucinostomus currani Zahuranec, 1980 in the Pacific Ocean off Costa Rica

Homalometron elongatum is reexamined using heat-killed material that was not subjected to pressure during fixation from Gerres cinereus collected from San Juan Harbor, Puerto Rico, U.S.A. The new material is compared with some paratype specimens and differs by having a much less variable forebody length, and a median rather than submedian genital pore. Tegumental spines reportedly cover the anterior end of the body but we observed tegumental spines covering the entire body surface in both the paratype and new material. Homalometron lesliorum n. sp. is described from Eucinostomus currani from the Pacific coasts of Costa Rica and Nicaragua. The new species has three pairs of oral papillae surrounding the mouth and thus resembles three other congeners: H. elongatum, Homalometron carapevae, and Homalometron papilliferum. Homalometron lesliorum n. sp. is distinguished from the three species by having the anterior extent of the vitelline follicles at or above the base of the ventral sucker, compared with posterior to the ventral sucker at the level of the seminal vesicle (H. elongatum) or further posterior at the posterior margin of the ovary (H. carapevae and H. papilliferum). The four species are further differentiated from one another by sucker width ratio, tegumental spine size and distribution, egg size, host preference, and biogeography. Comparison of nuclear ribosomal DNA (3' end of 18S, internal transcribed spacer [ITS]1, ITS2, and 5' end of 28S) between H. elongatum and H. lesliorum n. sp. revealed one variable base (n = 162) at the 3' end of 18S, 12 variable bases (n = 476) at ITS1, 10 variable bases (n = 310) at ITS2, and 11 variable bases (n = 1,325) at the 5' end fragment of 28S. Nuclear ribosomal DNA from Homalometron pallidum and Homalometron armatum are included for further comparison with H. elongatum and H. lesliorum n. sp.

Distribution of Dioecious Eastern Red Cedar (Juniperus virginiana) along an Environmental Gradient in Ogallala, NE.

Abstract The purpose of this research was to study the sex distribution and energy allocation of dioecious Eastern Red Cedars (Juniperus virginiana) along an environmental resource gradient. The trees surveyed were growing in a canyon located at the University of Nebraska’s Cedar Point Biological Research Station in Ogallala, Nebraska. Due to the geography of this canyon, environmental factors necessary for plant growth should vary depending on the tree’s location within the canyon. These factors include water availability, sun exposure, ground slope, and soil nitrogen content, all of which are necessary for carbon acquisition. Juniperus virginiana is a dioecious conifer. Dioecious plants maintain male and female reproductive structures on separate individuals. Therefore, proximal spatial location is essential for pollination and successful reproduction. Typically female reproductive structures are more costly and require a greater investment of carbon and nitrogen. For this reason, growth, survival and successful reproduction are more likely to be limited by environmental resources for females than for male individuals. If this is true for Juniperus virginiana, females should be located in more nutrient and water rich areas than males. This also assumes that females can not be reproductively successful in areas of poor environmental quality. Therefore, reproductive males should be more likely to inhabit environments with relatively lower resource availability than females. Whether the environment affects sexual determination or just limits survival of different sexes is still relatively unknown. In order to view distribution trends along the environmental gradient, the position of the tree in the canyon transect was compared to its sex. Any trend in sex should correspond with varying environmental factors in the canyon, ie: sunlight availability, aspect, and ground slope. The individuals’ allocation to growth and reproduction was quantified first by comparing trunk diameter at six inches above ground to sex and location of the tree. The feature of energy allocation was further substantiated by comparing carbon and nitrogen content in tree leaf tissue and soil to location and sex of each individual. Carbon and nitrogen in soil indicate essential nutrient availability to the individual, while C and N in leaf tissue indicate nutrient limitation experienced by the tree. At the conclusion of this experiment, there is modest support that survival and fecundity of females demands environments relatively richer in nutrients, than needed by males to survive and be reproductively active. Side of the canyon appeared to have an influence on diameter of trees, frequency of sex and carbon and nitrogen leaf content. While this information indicated possible trends in the relation of sex to nutrient availability, most of the environmental variables presumed responsible for the sex distribution bias differed minutely and may not have been biologically significant to tree growth.