965 resultados para Crossing experiments, Baculoviridae, Yeast Two-Hybrid System, Resistance management, sex-linkage


Relevância:

100.00% 100.00%

Publicador:

Resumo:

We demonstrate modulations of electrical conductance and hysteresis behavior in ZnO nanowire transistors via electrically polarized switching of ferroelectric liquid crystal (FLC). After coating a nanowire channel in the transistors with FLCs, we observed large increases in channel conductance and hysteresis width, and a strong dependence of hysteresis loops on the polarization states associated with the orientation of electric dipole moments along the direction of the gate electric field. Furthermore, the reversible switching and retention characteristics provide the feasibility of creating a hybrid system with switch and memory functions. © 2013 American Institute of Physics.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

Turbine design engineers have to ensure that film cooling can provide sufficient protection to turbine blades from the hot mainstream gas, while keeping the losses low. Film cooling hole design parameters include inclination angle (a), compound angle (b), hole inlet geometry, and hole exit geometry. The influence of these parameters on aerodynamic loss and net heat flux reduction is investigated, with loss being the primary focus. Low-speed flat plate experiments have been conducted at momentum flux ratios of IR=0.16, 0.64, and 1.44. The film cooling aerodynamic mixing loss, generated by the mixing of mainstream and coolant, can be quantified using a three-dimensional analytical model that has been previously reported by the authors. The model suggests that for the same flow conditions, the aerodynamic mixing loss is the same for holes with different a and b but with the same angle between the mainstream and coolant flow directions (angle k). This relationship is assessed through experiments by testing two sets of cylindrical holes with different a and b: one set with k=35 deg, and another set with k=60 deg. The data confirm the stated relationship between α, β, k and the aerodynamic mixing loss. The results show that the designer should minimize k to obtain the lowest loss, but maximize b to achieve the best heat transfer performance. A suggestion on improving the loss model is also given. Five different hole geometries (α=35.0 deg, β=0 deg) were also tested: cylindrical hole, trenched hole, fan-shaped hole, D-Fan, and SD-Fan. The D-Fan and the SD-Fan have similar hole exits to the fan-shaped hole but their hole inlets are laterally expanded. The external mixing loss and the loss generated inside the hole are compared. It was found that the D-Fan and the SD-Fan have the lowest loss. This is attributed to their laterally expanded hole inlets, which lead to significant reduction in the loss generated inside the holes. As a result, the loss of these geometries is≈50% of the loss of the fan-shaped hole at IR=0.64 and 1.44. © 2013 by ASME.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

In mammals, the development of reflexes is often regarded as an innate process. However, recent findings show that fetuses are endowed with favorable conditions for ontogenetic development. In this article, we hypothesize that the circuitry of at least some mammalian reflexes can be self-organized from the sensory and motor interactions brought forth in a musculoskeletal system. We focus mainly on three reflexes: the myotatic reflex, the reciprocal inhibition reflex, and the reverse myotatic reflex. To test our hypothesis, we conducted a set of experiments on a simulated musculoskeletal system using pairs of agonist and antagonist muscles. The reflex connectivity is obtained by producing spontaneous motor activity in each muscle and by correlating the resulting sensor and motor signals. Our results show that, under biologically plausible conditions, the reflex circuitry thus obtained is consistent with that identified in relation to the analogous mammalian reflexes. In addition, they show that the reflex connectivity obtained depends on the morphology of the musculoskeletal system as well as on the environment that it is embedded in.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

Using remote sensing technique, we investigated real-time Nostoc sphaeroides Kiltz (Cyanobacterium) in Closed System under microgravity by SHENZHOU-2 spacecraft in January 2001. The experiments had 1g centrifuges in space for control and ground control group experiments were also carried out in the same equipments and under the same controlled condition. The data about the population growth of Nostoc sp. of experiments and temperature changes of system were got from spacecraft every minute. From the data, we can find that population growth of Nostoc sp. in microgravity group was higher than that of other groups in space or on ground, even though both the control I g group in space and I g group on ground indicated same increasing characteristics in experiments. The growth rate of 1.4g group (centrifuged group on ground) was also promoted during experiment. The temperature changes of systems are also affected by gravity and light. Some aspects about those differences were discussed. From the discussion of these results during experiment, it can be found that gravity is the major factor to lead to these changes. (C) 2004 Elsevier Ltd. All rights reserved.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

Variations in optical spectrum and modulation band-width of a modulated Fabry-Perot (FP) semiconductor laser subject to the external light injection from another FP Laser is investigated in this paper. Optimal wavelength matching conditions for two FP lasers are discussed. A series of experiments show that two FP lasers should have a central wavelength overlapping and a mode spacing difference of several gigahertz. Under these conditions both the magnitude and phase frequency responses can be improved significantly.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

By employing non-equilibrium Green's function method, the mesoscopic Fano effect modulated by Rashba spin-orbit (SO) coupling and external magnetic field has been elucidated for electron transport through a hybrid system composed of a quantum dot (QD) and an Aharonov-Bohm (AB) ring. The results show that the orientation of the Fano line shape is modulated by the Rashba spin-orbit interaction k(R)L variation, which reveals that the Fano parameter q will be extended to a complex number, although the system maintains time-reversal symmetry (TRS) under the Rashba SO interaction. Furthermore, it is shown that the modulation of the external magnetic field, which is applied not only inside the frame, but also on the QD, leads to the Fano resonance split due to Zeeman effect, which indicates that the hybrid is an ideal candidate for the spin readout device. (C) 2007 Elsevier B.V All rights reserved.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

BRUNOL/CELF家族RNA结合蛋白在转录后调控(post-transcriptional regulation)中起着至关重要的作用,参与多种组织的发育过程。本研究中,我们描述了非洲爪蟾5个Brunol 基因的克隆与表达。其中只有Brunol2是母源性以及合子表达的,其它的4个Brunol基因都是合子表达的,但起始表达的发育时期有所不同。爪蟾发育过程中,Brunol1、4和5基因特异性地在神经系统中表达,包括脑、脊髓、眼泡和耳泡。Brunol2和3基因在体节中胚层与神经系统表达。Brunol2也在晶状体中有非常高的表达。在转染的Hela细胞中,BRUNOL1、2和3蛋白定位于细胞质和细胞核中,BRUNOL4和5只是定位于细胞质中,显示它们具有不同的功能。 人的microcephalin1(MCPH1)基因的遗传突变产生原发性小头症,而在人类的进化过程中,这个基因的变化可能对人脑体积的增加和认知能力的增强也起到重要的作用。但是对于MCPH基因在其它物种中功能的研究才刚刚开始。我们克隆了非洲爪蟾MCPH基因,发现非洲爪蟾具有A,B两个同源基因,其功能域的保守性较高,暗示非洲爪蟾MCPH基因仍然执行一些保守的功能,但MCPHB由于突变只编码一种截短的蛋白,目前尚不清楚它是否是有功能的。胚胎原位杂交的结果显示MCPHA,B基因在胚胎发育中的表达图式相似,但MCPHB的表达水平较低。在神经胚期,二者均表达于头部基板区,在尾芽期主要表达于咽鳃区,而在脑区的表达并不显著,与小鼠中的表达模式不同,提示在爪蟾中MCPH基因可能主要参与咽鳃区而不是脑的发育。 为了进一步筛选这些蛋白可能的结合因子,我们构建了非洲爪蟾双杂交cDNA文库。其中利用修饰的随机引物和特别设计的连接头在合成双链cDNA时,在下游合成一个SalI限制性酶切位点,可以将cDNA定向插入载体。通过对于空克隆率和插入片段长度等一系列参数的分析,表明这个定向cDNA文库的构建是成功的。

Relevância:

100.00% 100.00%

Publicador:

Resumo:

本研究应用微波消解ICP-AES 法对62 个小麦品种及3 个地区土壤的锌铁硒含量进行了分析测定,发现不同小麦品种中微量元素含量差异很大,姊妹系间也存在差异。含铁量最高与最低的小麦品种铁含量相差29.68mg/kg。含锌量最高与最低的小麦品种锌含量相差46.70 mg/kg。含硒量最高与最低的小麦品种硒含量相差0.056 mg/kg。对不同地点的小麦及土壤中锌铁硒含量进行方差分析,发现双流和西昌两地种植小麦的铁含量和硒含量均有显著差异,西昌和荣县种植的锌含量有显著差异。在3 个地点中双流种植小麦硒含量最高,西昌种植小麦的铁和锌含量最高。 通过对小麦微量元素含量与土壤中微量元素含量进行了相关性分析,结果表明:小麦中的锌铁含量与土壤中的锌铁含量呈显著正相关,土壤中铁与锌含量呈极显著正相关,小麦中铁与锌含量也呈极显著正相关。随着土壤微量元素锌铁的提高,小麦中的锌铁元素含量同时提高,而且小麦对两种元素的吸收互相促进。土壤中的硒含量与锌铁含量呈负相关。小麦中硒含量也与锌铁含量也呈负相关。说明锌和铁与硒互相拮抗。小麦硒含量与土壤硒含量呈正相关,但不显著。表明土壤硒含量可以影响小麦硒含量,但不是决定因素,小麦硒含量与小麦自身因素有关。 对姊妹系G290(高硒含量)和G289(低硒含量)进行抗重金属胁迫和抗旱性实验发现,高硒品种G290的抗逆性优于低硒品种G289。 利用RAPD 技术对7 个姊妹系进行遗传差异分析发现,高硒材料G290出现了特异条带,分别标为1、2、3、4,其他姊妹系品种中未发现特异条带,回收4 条特异条带并连接转化,得到目的片段1、2、3 的重组子,进行测序。NCBI 中结果显示没有找到植物中的同源序列,说明特异序列可能是未发现的基因片段,推测可能与小麦硒含量有关,有待进一步研究。 以上研究结果,对小麦营养研究及功能性小麦的筛选和栽培具有指导作用。 In this study, we determinated the contents of zinc, iron, selenium in 62 wheat cultivars and soil samples of three regions by method of microwave digestion/ ICPAES,found that there was great difference of zinc, iron, selenium contents in different wheat cultivars as well as different sister lines. Iron content difference was 29.68 mg/kg between the highest-iron-content cultivar and the lowest one, and zinc content difference was 46.70 mg/kg , selenium content difference was 0.056 mg/kg. Anova analysis was made on contents of zinc, iron, selenium in wheat and soil samples of different locations, significant differences of Fe and Se contents were found between wheat in Shuangliu and Xichang, significant difference of Zn content was found between wheat in Xichang and Rongxian. Se content in wheat of Shuangliu was highest, Fe and Zn contents in wheat of Xichang were highest. Relativity analysis was made on three trace elements in Wheat and in soil, the result showed that there was significant positive correlation of zinc, iron content between in Wheat and in soil, as well as between Fe and Zn both in wheat and in soil. With the improving of Zn, Fe contents in soil, contents of Zn and Fe in wheat increased and absorption of Zn and Fe in wheat will mutual promote. Negative correlation of Se and Zn contents was found in wheat and soil, but not significant, that meant the antagonism of Se and Zn. Positive correlation of Se content in wheat and soil was found. High selenium content G290 and low selenium content G289 in sister lines were selected for heavy metal stress and drought resistance experiments, the result showed that the resistance of high-selenium-content cultivar was better than low selenium one. Analysis on genetic difference was made by RAPD, and specific bands were selected, marked 1,2,3,4, no more specific bands were found in other sister lines.4 bands were recovered, ligated to T-vector and transformed E.coli. Three recombinant plasmids were obtained and sequenced. NCBI Blast showed there was no homology with other plants. It implied that these fragments probably be new genes and maybe were related to selenium in wheat. It needs further research. This paper would be useful for the study of wheat nutrition as well as selection and cultivation of functional wheat.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

With a latest developed electric-sweep scanner system, we have done a lot of experiments for studying this scanner system and ion beam emittance of electron cyclotron resonance (ECR) ion source. The electric-sweep scanner system was installed on the beam line of Lanzhou electron resonance ion source No. 3 experimental platform of Institute of Modem Physics. The repetition experiments have proven that the system is a relatively dependable and reliable emittance scanner, and its experiment error is about 10%. We have studied the influences of the major parameters of ECR ion source on the extracted ion beam emittance. The typical results of the experiments and the conclusions are presented in this article.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

Inorganic nanoparticles (NPs) with attractive electronic, optical, magnetic, thermal and catalytic properties have attracted great interest due to their important applications in physics, chemistry, biology, medicine, materials science and interdisciplinary fields. Biomolecule-NP hybrid systems, which combine recognition and catalytic properties of biomolecules with electronic, optical, magnetic and catalytic properties of NPs, are particularly new materials with synergistic properties originating from the components of the hybrid composites. The biomolecule-NP hybrid system has excellent prospects for interfacing biological recognition events with electronic signal transduction so as to design a new generation of bioelectronic devices with high sensitivity.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

For the first time, a novel prefractionation method used in proteomic analysis was developed, which is performed by a novel aqueous two-phase system (NATPS) composed of n-butanol, (NH4)(2)SO4, and water. It can separate proteomic proteins into multigroups by one-step extraction. The phase-separation conditions of n-butanol solutions were studied in the presence of commonly used inorganic salts. The NATPS was subsequently developed. Using human serum albumin, zein, and gamma-globulin as model proteins, the separation effectiveness of the NATPS for protein was studied under affection factors, i.e., pH, n-butanol volume, protein, or salt concentration. The model and actual protein samples were separated by the NATPS and then directly used for gel electrophoresis without separating the target proteins from phase-forming reagents. It revealed that the NATPS could separate proteomic proteins into multigroups by one-step extraction. The NATPS has the advantages of rapidity, simplicity, low cost, biocompability, and high efficiency. It need not separate target proteins from the phase-forming reagents. The NATPS has great significance in separation and extraction of proteomic proteins, as well as in methodology.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

The spherical Lindquist type polyoxometalate, Mo6O192-, has been used as a noncoordinating anionic template for the construction of novel three-dimensional lanthanide-aromatic monocarboxylate dimer supramolecular networks [Ln(2)(DNBA)(4)(DMF)(8)][Mo6O19] (Ln = La 1, Ce 2, and Eu 3, DNBA = 3,5-dinitrobenzoate, DMF = dimethylformamide). The title compounds are characterized by elemental analyses, IR, and single-crystal X-ray diffractions. X-ray diffraction experiments reveal that two Ln(III) ions are bridged by four 3,5-dinitrobenzoate anions as asymmetrically bridging ligands, leading to dimeric cores, [Ln(2)(DNBA)(4)(DMF)(8)](2+); [Ln(2)(DNBA)(4)(DMF)(8)](2+) groups are joined together by pi-pi stacking interactions between the aromatic groups to form a two-dimensional grid-like network; the 2-D supramolecular layers are further extended into 3-D supramolecular networks with 1-D box-like channels by hydrogen-bonding interactions, in which hexamolybdate polyanions reside. The compounds represent the first examples of 3-D carboxylate-bridged lanthanide dimer supramolecular "host" networks formed by pi-pi stacking and hydrogen-bonding interactions encapsulating noncoordinating "guest" polyoxoanion species. The fluorescent activity of compound 3 is reported.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

Poly(ethylene oxide) (PEO) and silica (SiO2) organic-inorganic hybrid materials have been synthesized by sol-gel approach. The crystallization behavior of PEO in silica networks has been investigated by differential scanning calorimeter (DSC) and scanning electron micrograph (SEM). The degree of PEO crystallinity in PEO/SiO2 hybrid networks reduces with the increase of SiO2. PEO is in amorphous state when the concentration of PEO is lower than 50 wt% in the hybrid materials. The melting points of PEO in the networks are lower than that of pure PEG, but the melting point of PEO in the networks almost has the same melting point. WAXD and SEM results show that the crystalline behavior of PEO in PEO/SiO2 hybrid system is sternly confined. (C) 1999 Elsevier Science Ltd. All rights reserved.

Relevância:

100.00% 100.00%

Publicador:

Resumo:

A blend of polyethersulfone (PES) and polycarbonate (PC) with a ratio of 40/60 was studied by scanning electron microscopy (SEM), dynamic mechanical analysis, and transmission electron microscopy (TEM). It was found that the PES-PC blend is a partially miscible, two-phase system, and an interfacial layer exists between the phases of PES and PC. Specific interaction resulting from the n-complex between PES and PC provides the driving force for formation of the interfacial layer. In addition, phase inversion behavior was also observed for the 40/60 composition.