986 resultados para Chromosome 29
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Las actas recogen lasponencias y comunicaciones as?? como las conclusiones de los grupos de trabajo
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Primer Congresos sobre agua y educaci??n ambiental cuyo objetivo es servir de foro de encuentro e intercambio de experiencias entre profesionales de la educaci??n ambiental y otros agentes institucionales, sociales y empresarios implicados en la gesti??n y el tratamiento del recurso del agua. Los temas abordados fueron: el agua en la escuela (acciones y recursos educativos) educaci??n ambiental para el agua (convenios, directivas y estrategias de desarrollo sostenible) el papel de la administraci??n, la participaci??n ciudadana, la integraci??n de los agentes sociales en la educaci??n ambiental, el papel de la empresa y de los medios de comunicaci??n.
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Las ponencias se presentan en formato PDF. - Se ofrece un enlace al listado de publicaciones realizadas por los asistentes al seminario.
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We have developed a system to hunt and reuse special gene integration sites that allow for high and stable gene expression. A vector, named pRGFP8, was constructed. The plasmid pRGFP8 contains a reporter gene, gfp2 and two extraneous DNA fragments. The gene gfp2 makes it possible to screen the high expression regions on the chromosome. The extraneous DNA fragments can help to create the unique loci on the chromosome and increase the gene targeting frequency by increasing the homology. After transfection into Chinese hamster ovary cells (CHO) cells, the linearized pRGFP8 can integrate into the chromosome of the host cells and form the unique sites. With FACS, 90 millions transfected cells were sorted and the cells with strongest GFP expression were isolated, and then 8 stable high expression GFP CHO cell lines were selected as candidates for the new host cell. Taking the unique site created by pRGFP8 on the chromosome in the new host cells as a targeting locus, the gfp2 gene was replaced with the gene of interest, human ifngamma, by transfecting the targeting plasmid pRIH-IFN. Then using FACS, the cells with the dimmest GFP fluorescence were selected. These cells showed they had strong abilities to produce the protein of interest, IFN-gamma. During the gene targeting experiment, we found there is positive correlation between the fluorescence density of the GFP CHO host cells and the specific production rate of IFN-gamma. This result shows that the strategy in our expression system is correct: the production of the interesting protein increases with the increase fluorescence of the GFP host cells. This system, the new host cell lines and the targeting vector, can be utilized for highly expressing the gene of interest. More importantly, by using FACS, we can fully screen all the transfected cells, which can reduce the chances of losing the best cells.
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Nova et Vetera, ISSN 1692-5866, Año 8, No. 01 (Enero 29 - Febrero 10 de 2008)
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Nova et Vetera, ISSN 1692-5866, Año 3, No. 10 (Julio 29 - Agosto 10 de 2008)
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Nova et Vetera, ISSN 1692-5866, Año 4, No. 04 (Marzo 16 - Marzo 29 de 2009)
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Nova et Vetera, ISSN 1692-5866, Año 5, No. 11 (Agosto 17 - 29 de 2010)
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Decano de la Facultad de Economía estará fuera del país, por razones de trabajo, Que, a fin de que la Decanatura de Economía esté debidamente atendida, es necesario proveer un encargo temporal.
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Nova et Vetera, ISSN 1692-5866, Año 6, No. 19 (Agosto 29 de 2011)
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Nova et Vetera, ISSN 1692-5866, Año 6, No. 29 (Noviembre 15 de 2011)
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Nova et Vetera, ISSN 1692 - 5866, Año 7 No. 28 (Octubre 29 - enero 27 de 2012)