Effects of the antimicrobial peptide gomesin on the global gene expression profile, virulence and biofilm formation of Xylella fastidiosa

In the xylem vessels of susceptible hosts, such as citrus trees, Xylella fastidiosa forms biofilm-like colonies that can block water transport, which appears to correlate to disease symptoms. Besides aiding host colonization, bacterial biofilms play an important role in resistance against antimicrobial agents, for instance antimicrobial peptides (AMPs). Here, we show that gomesin, a potent AMP from a tarantula spider, modulates X. fastidiosa gene expression profile upon 60 min of treatment with a sublethal concentration. DNA microarray hybridizations revealed that among the upregulated coding sequences, some are related to biofilm production. In addition, we show that the biofilm formed by gomesin-treated bacteria is thicker than that formed by nontreated cells or cells exposed to streptomycin. We have also observed that the treatment of X. fastidiosa with a sublethal concentration of gomesin before inoculation in tobacco plants correlates with a reduction in foliar symptoms, an effect possibly due to the trapping of bacterial cells to fewer xylem vessels, given the enhancement in biofilm production. These results warrant further investigation of how X. fastidiosa would respond to the AMPs produced by citrus endophytes and by the insect vector, leading to a better understanding of the mechanism of action of these molecules on bacterial virulence.

Fallolyckor i äldre personers egna hem : en retrospektiv registerstudie

Syftet med studien var att undersöka hur fallfrekvensen var fördelad mellan män och kvinnor, hur antalet fall var fördelade i åldersgrupperna över respektive under 80 år, när de flesta fallolyckor i det egna hemmet inträffade och om fallen medförde några konsekvenser.Studien var en retrospektiv registerstudie och inkluderade fall hos personer i eget boende med trygghetslarm och/eller hjälp av nattpatrull. Fallolyckorna registrerades på ett instrument som var speciellt utvecklat för projektet. Data från en sex månaders studie med totalt 510 fall av 213 individer analyserades. Resultatet visade att det var främst äldre personer över 80 år som föll och som hamnade på sjukhus. Fallen inträffade oftast förmiddagar och eftermiddagar. Få fall inträffade mellan klockan 00-09. Kvinnorna föll framförallt under december månad. Antal fall som ledde till sjukhusvård var 4.5 % och av dessa var nästan alla individer över 80 år och flertalet var kvinnor. Av de fall som ledde till sjukhusvård inträffade 39 % vid första och enda fallet. Då många vårdtagare föll ett flertal gånger och antalet fall kunde vara mycket högt bör de fallförebyggande åtgärderna ses över. Downton fallriskindex skulle kunna vara ett komplement till övriga fallpreventioner i hemmet. Studien ger användbar data med möjlighet att till exempel utforma hemtjänstens schema utifrån studieresultatet och på så sätt minska antalet fallolyckor i hemmen.

Energy Efficiency through Thermal Energy Storage - Evaluation of the Possibilities for the Swedish Building Stock, Phase 1

As a first step in assessing the potential of thermal energy storage in Swedish buildings, the current situation of the Swedish building stock and different storage methods are discussed in this paper. Overall, many buildings are from the 1960’s or earlier having a relatively high energy demand, creating opportunities for large energy savings. The major means of heating are electricity for detached houses and district heating for multi dwelling houses and premises. Cooling needs are relatively low but steadily increasing, emphasizing the need to consider energy storage for both heat and cold. The thermal mass of a building is important for passive storage of thermal energy but this has not been considered much when constructing buildings in Sweden. Instead, common ways of storing thermal energy in Swedish buildings today is in water storage tanks or in the ground using boreholes, while latent thermal energy storage is still very uncommon.

"Vos sos la mujer araña, que atrapa a los hombres en su tela" : La construcción de la identidad feminina en El beso de la Mujer araña, de Manuel Puig.

La novela El beso de la mujer araña del escritor argentino Manuel Puig es una novela que trata dedos presos en una cárcel bonaerense durante la dictadura militar argentina. Esta tesina se centrará enla identidad femenina de Molina. El objetivo de esta tesina es examinar la celebración de lafemineidad de Molina, y su construcción de una identidad femenina. Esta identidad se conectarácon la idea de la identidad femenina como socialmente construida, opuesto a la noción de génerocomo algo innato. Es decir, la identidad femenina de Molina y sus ideas de lo femeninocorresponden a la idea de la identidad femenina como socialmente construida. Apoyándonos enteoría feminista y teoría queer, estableceremos la conexión que existe entre la identidad femeninacomo la ve Molina, y la identidad femenina como construcción social.

Om trångboddhet : Hur storleken på våra bostäder blev ett välfärdsproblem

About crowding – How the size of our dwellings became a welfare problem Housing policy documents have traditionally been studied by political scientists, resulting in a lack of interest in the private aspects of housing policy. Hence, this paper uses the example of crowding standards to examine how a previously private matter, the size of our dwelling, became a concern of the state. Official governmental documents are analyzed with the help of discourse theory, working on the supposition that the need of the population and the framing of a problem changes over time. The first official standard of crowding, formulated in 1946 argue for larger dwelling size in order to increase the size and quality of the Swedish population. The second standard, formulated in 1965, is based on the assumption that the population, defined as consumers, demands larger sized homes. The final standard, formulated in 1975, claims that larger sized homes is a social right.

Evaluation of a thermally driven heat pump for solar heating and cooling applications

Exploiting solar energy technology for both heating and cooling purposes has the potential of meeting an appreciable portion of the energy demand in buildings throughout the year. By developing an integrated, multi-purpose solar energy system, that can operate all twelve months of the year, a high utilisation factor can be achieved which translates to more economical systems. However, there are still some techno-economic barriers to the general commercialisation and market penetration of such technologies. These are associated with high system and installation costs, significant system complexity, and lack of knowledge of system implementation and expected performance. A sorption heat pump module that can be integrated directly into a solar thermal collector has thus been developed in order to tackle the aforementioned market barriers. This has been designed for the development of cost-effective pre-engineered solar energy system kits that can provide both heating and cooling. This thesis summarises the characterisation studies of the operation of individual sorption modules, sorption module integrated solar collectors and a full solar heating and cooling system employing sorption module integrated collectors. Key performance indicators for the individual sorption modules showed cooling delivery for 6 hours at an average power of 40 W and a temperature lift of 21°C. Upon integration of the sorption modules into a solar collector, measured solar radiation energy to cooling energy conversion efficiencies (solar cooling COP) were between 0.10 and 0.25 with average cooling powers between 90 and 200 W/m2 collector aperture area. Further investigations of the sorption module integrated collectors implementation in a full solar heating and cooling system yielded electrical cooling COP ranging from 1.7 to 12.6 with an average of 10.6 for the test period. Additionally, simulations were performed to determine system energy and cost saving potential for various system sizes over a full year of operation for a 140 m2 single-family dwelling located in Madrid, Spain. Simulations yielded an annual solar fraction of 42% and potential cost savings of €386 per annum for a solar heating and cooling installation employing 20m2 of sorption integrated collectors.

‘Moving on’ : Violence, wellbeing and questions about violence in antenatal care encounters. A qualitative study with Somali-born refugees in Sweden

Background Somali-born women constitute one of the largest groups of childbearing refugee women in Sweden after more than two decades of political violence in Somalia. In Sweden, these women encounter antenatal care that includes routine questions about violence being asked. The aim of the study was to explore how Somali-born women understand and relate to violence and wellbeing during their migration transition and their views on being approached with questions about violence in Swedish antenatal care. Method Qualitative interviews (22) with Somali-born women (17) living in Sweden were conducted and analysed using thematic analysis. Findings A balancing actbetween keeping private life private and the new welfare system was identified, where the midwife's questions about violence were met with hesitance. The midwife was, however, considered a resource for access to support services in the new society. A focus on pragmatic strategies to move on in life, rather than dwelling on potential experiences of violence and related traumas, was prominent. Social networks, spiritual faith and motherhood were crucial for regaining coherence in the aftermath of war. Dialogue and mutual adjustments were identified as strategies used to overcome power tensions in intimate relationships undergoing transition. Conclusions If confidentiality and links between violence and health are explained and clarified during the care encounter, screening for violence can be more beneficial in relation to Somali-born women. The focus on “moving on” and rationality indicates strength and access to alternative resources, but needs to be balanced against risks for hidden needs in care encounters. A care environment with continuity of care and trustful relationships enhances possibilities for the midwife to balance these dual perspectives and identify potential needs. Collaborations between Somali communities, maternity care and social service providers can contribute with support to families in transition and bridge gaps to formal social and care services.

Índices de avaliação de processos : abordagem univariada e multivariada

Esta dissertação versa sobre índices de avaliação de processo nas suas abordagens univariada e multivariada. Analisa-se a utilização da Análise de Componentes Principais (ACP) como ferramenta no desenvolvimento de índices capazes de avaliar processos multivariados. O trabalho apresenta uma revisão teórica sobre os índices univariados de aplicação mais comum na indústria (CP/PP , CPK/PPK , CPM/PPM e CPMK/PPMK), o índice multivariado MCpm e sobre os índices MCP , MCPK , MCPM e MCPMK , associados a ACP. Os índices de avaliação de processo são analisados quanto à sua adequação ao uso, através de um estudo de caso na indústria de componentes automotivos. Para tanto, examina-se o processo de fabricação de um componente do freio de veículos médios e pesados, denominado spider, em que doze variáveis de processo são controladas por meio de controle estatístico de processo. Através do estudo de caso, faz-se uma comparação entre os resultados dos índices univariados e multivariados; pressupõe-se que através da Análise de Componentes Principais poder-se-á concluir sobre a capacidade e o desempenho de processos multivariados. Por fim, a partir da análise univariada dos componentes principais, apresenta-se uma técnica complementar para quantificar a contribuição das variáveis controladas à variabilidade de processos multivariados.

Propagação de espécies nativas com potencial ornamental : Kelissa brasiliensis (Baker) Ravenna e Sinningia lineata (Hjelmq.) Chautems

A pesquisa em tecnologia de sementes contribui para a manutenção de bancos de germoplasma. Kelissa brasiliensis (Baker) Ravenna e Sinningia lineata (Hjelmq.) Chautems são espécies nativas com potencial ornamental. Os objetivos deste trabalho foram desenvolver testes para a germinação de sementes, conhecer a qualidade fisiológica de lotes coletados in situ, desenvolver protocolos de propagação vegetativa e conhecer estratégias reprodutivas no ambiente. Avaliaram-se o vigor, bem como o comportamento germinativo das sementes em diferentes substratos, temperaturas e condições de luz. Foram realizados experimentos com propagação vegetativa de S. lineata. O trabalho foi conduzido no Laboratório de Sementes da Fepagro, no Jardim Botânico de Porto Alegre e na Faculdade de Agronomia da UFRGS. Durante as coletas, foi feita a observação dos nichos das espécies. O delineamento experimental foi completamente casualizado com quatro repetições de 10 e 20 sementes para K. brasiliensis e S. lineata, respectivamente; para S. lineata, três tratamentos com 17 repetições foram utilizados no experimento com estaquia, e nove tratamentos com quatro repetições para o teste de divisão dos tubérculos. A comparação das médias foi realizada através do teste de Duncan (P<0,05). A temperatura mais adequada para a germinação de sementes de K. brasiliensis foi 10ºC, o que explica a sua restrita distribuição ao bioma Pampa; a combinação 24h/25ºC levou a uma liberação maior de lixiviados na condutividade elétrica; o envelhecimento acelerado (72 h em 41ºC e 100% de umidade) não provocou redução significativa no percentual de germinação das sementes de K. brasiliensis. Já as sementes de S. lineata não germinaram após serem expostas ao estresse. A temperatura mais adequada para o teste de germinação de sementes de S. lineata foi 20ºC; para ambas espécies, o substrato papel e a presença de luz foram as condições mais adequadas para a germinação. S. lineata demonstrou ser facilmente propagada por sementes e via assexuada, o que revelou a rusticidade característica das espécies rupícolas.

Interface entre arquitetura bioclimática e decisões projetuais

This research has as its object study focus bioclimatic in architecture and its conection with projects decisions, on what regards to environmental comfort for single-family dwelling. From the analysis of five architectural projects inserted in Natal/RN, warm-moist weather, this research gather informations regarding architectural features guided by shape and space arrengement, which embody important elements for the project design development. Computer simulations assisted as foundation to verify the efficiency grade for these projects strategies from shading analysis. Related strategies for the demands of natural ventilation circulation and thermal mass for refrigeration were analysed as well. Results show that there is an hierarchizing of priorities for the decisions made when it comes to shape and space disposition variables, as well as the way these variables will consider the bioclimatic demands. The analysis, even, show that there is no single way to respond to specific bioclimatic demands, as it points out the value of examination of the projectual solutions throughtout the conception process, in order to achieve an efficient project performance for the envimonment comfort

Exclusão social e projetos habitacionais. Um estudo sobre conjuntos habitacionais, segregação e exclusão social em Natal/RN

In Natal s urban growth process it is given that the performance period of the National Housing Bank (BNH, 1964-1986) was marked by the intense expansion of the urban grid and configuration of outskirts, through the construction of social housing developments. Implanted in segregated areas of the existing formal city, the population installed in these complexes was also excluded from their rights, considering that the housing defines itself not only by the physical dwelling, but also by its access to urban infrastructure, facilities, services, and others. From this reality and the verification of the city s exclusion and sociospatial segregation processes, we aimed to quantitatively demonstrate levels of social exclusion in Natal, based on the methodology developed by Sposati (2000) and adapted by Genovez (2002), which relates IBGE s (Brazilian Institute of Geography and Statistics) database underlying variables such as income, schooling and dwelling s quality. The research unveiled some spatial patterns promoted by the social housings: in these areas islands were developed with higher indicators than surrounding areas, revealing internal hierarchies in the city s outskirts

Larval development of Notolopas brasiliensis Miers, 1886 (Brachyura: Majoidea: Pisidae) described from laboratory reared material and a reappraisal of the characters of Pisidae

O completo desenvolvimento larval de Notolopas brasiliensis é descrito, a partir de material criado em laboratório, com ênfase na morfologia externa de Majoidea e comparado aos demais gêneros de Pisidae. O desenvolvimento larval de N. brasiliensis consiste em dois estágios de zoea e um de megalopa. A duração media de cada estágio foi de 4.2 ± 1.0 dias para a Zoea I e 3.8 ± 0.7 dias para a Zoea II, a megalopa aparece entre 8.1 ± 0.4 dias após a eclosão. Os caracteres previamente utilizados para definir as formas larvais de Pisidae ou são simplesiomórficos ou altamente homoplásticos. Foi observado que não existe um conjunto de caracteres capazes de definir Pisidae até o presente.Contudo foi mostrado que uma combinação de caracteres pode ser utilizada para diferenciar Notolopas dos demais gêneros da família.

Taxonomic redescription and biological notes on Diaugia angusta (Diptera,Tachinidae): parasitoid of the palm boring weevils Metamasius ensirostris and M. hemipterus (Coleoptera, Dryophthoridae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Níveis de homocisteína e desempenho cognitivo em uma amostra populacional de idosos da cidade do Natal-RN

The imprecision of the frontier that separates those cognitive deficits inherent to the human physiological aging process from those which represent the early signs of nervous system degenerative pathologies ,very prevalent among the elderly, has brought attention to the need of studies aiming to establish clinical and/or laboratorial criteria to allow this differentiation. Elderly people living in poor and developing countries are frequently exposed to precarious socioeconomic conditions which facilitate the development of an array of pathologies which have metabolic and nutritional dysfunctions as the established or proposed etiological agents. The levels of certain micronutrients, such as the vitamins B12 and B9 (folic acid), and of some intermediary metabolites, such as homocysteine are being thought of as etiological factors and/or as biological markers of a group of alterations which affect the normal functioning of the nervous system with important reflexes upon cognitive performance. This study aims to investigate the influence of homocysteine, B12 vitamin and folic acid levels on the cognitive performance of the low income elderly population. This transversal study took place in Natal, Rio Grande do Norte State, Brazil, and involved 205 dwelling elderly people, users of the Programa de Saúde da Família, a public healthcare program, maintained by the city s health authorities. A multidimensional questionnaire was used to assess the socio-demographic aspects and the overall health and nutrition conditions. The cognitive performance was measured by the use of the Portuguese version of the Mini Mental State Exam (MMSE). The serum levels of homocysteine, B12 vitamin and folic acid were determined by chemiluminescence. The association between the socio-demographic and serum levels of Hcy, B12 vitamin and folic acid was determined by multiple linear regression. Serum levels higher than 13.5 μmol/l, indicative of hyperhomocysteinemia (HHcy), were found on 25.4% of the sample, being more prevalent in men (p<0.05). Deficitary levels of folic acid (<5ng/mol) and of B12 vitamin (<193 pg/ml) were found on 3.9% and 10.2% of the sample respectively. A negative correlation was found between cognitive performance with both age and HHcy and a positive correlation was found between cognitive performance and schooling. The isolated HHcy R2 values were an explanation to only 4% of the variance of the MMSE scores. However, when associated with schooling and age, this model explains about 25% of this association