Sistema de gestión de un centro escolar

En este proyecto se abordará la implantación de un sistema informático que solicita un centro escolar. Inicialmente, este sistema no abarcará todo el proceso de gestión de la unidad educativa, sino que debe centrarse en optimizar la parte correspondiente al proceso de negocio que maneja el intercambio de información entre profesores y padres de alumnos.

Vanhasen tutkimus on kauttaaltaan virheellinen

Vastine emeritusprofessori Tatu Vanhasen yhteenvetoon tutkimustuloksistaan TT-lehdessä 6/2004

A combined computational and functional approach identifies new residues involved in pH-dependent gating of ASIC1a.

Acid-sensing ion channels (ASICs) are key receptors for extracellular protons. These neuronal nonvoltage-gated Na(+) channels are involved in learning, the expression of fear, neurodegeneration after ischemia, and pain sensation. We have applied a systematic approach to identify potential pH sensors in ASIC1a and to elucidate the mechanisms by which pH variations govern ASIC gating. We first calculated the pK(a) value of all extracellular His, Glu, and Asp residues using a Poisson-Boltzmann continuum approach, based on the ASIC three-dimensional structure, to identify candidate pH-sensing residues. The role of these residues was then assessed by site-directed mutagenesis and chemical modification, combined with functional analysis. The localization of putative pH-sensing residues suggests that pH changes control ASIC gating by protonation/deprotonation of many residues per subunit in different channel domains. Analysis of the function of residues in the palm domain close to the central vertical axis of the channel allowed for prediction of conformational changes of this region during gating. Our study provides a basis for the intrinsic ASIC pH dependence and describes an approach that can also be applied to the investigation of the mechanisms of the pH dependence of other proteins.

Millainen genomitietopankki Suomeen?

Vastine TT-lehden numerossa 8/2004 olleeseen Kirsti Käpyahon, Leena Peltonen-Palotien, Markus Perolan ja Tero Piispasen artikkeliin

UOC: Gestió Hospitalària

Desenvolupament d'una aplicació amb Microsoft.net per la gestió d'un hospital.

T cell receptor V beta repertoire in mice lacking endogenous mouse mammary tumor provirus.

When endogenous mouse mammary tumor virus (MMTV) superantigens (SAg) are expressed in the first weeks of life an efficient thymic deletion of T cells expressing MMTV SAg-reactive T cell receptor (TcR) V beta segments is observed. As most inbred mouse strains and wild mice contain integrated MMTV DNA, knowing the precise extent of MMTV influence on T cell development is required in order to study T cell immunobiology in the mouse. In this report, backcross breeding between BALB.D2 (Mtv-6, -7, -8 and -9) and 38CH (Mtv-) mice was carried out to obtain animals either lacking endogenous MMTV or containing a single MMTV locus, i.e. Mtv-6, -7, -8 or -9. The TcR V beta chain (TcR V beta) usage in these mice was analyzed using monoclonal antibodies specific for TcR V beta 2, V beta 3, V beta 4, V beta 5, V beta 6, V beta 7, V beta 8, V beta 11, V beta 12 and V beta 14 segments. Both Mtv-8+ mice and Mtv-9+ mice deleted TcR V beta 5+ and V beta 11+ T cells. Moreover, we also observed the deletion of TcR V beta 12+ cells by Mtv-8 and Mtv-9 products. Mtv-6+ and Mtv-7+ animals deleted TcR V beta 3+ and V beta 5+ cells, and TcR V beta 6+, V beta 7+ and V beta 8.1+ cells, respectively. Unexpectedly, TcR V beta 8.2+ cells were also deleted in some backcross mice expressing Mtv-7. TcR V beta 8.2 reactivity to Mtv-7 was shown to be brought by the 38CH strain and to result from an amino acid substitution (Asn-->Asp) in position 19 on the TcR V beta 8.2 fragment. Reactivities of BALB.D2 TcR V beta 8.2 and 38CH TcR V beta 8.2 to the exogenous infectious viruses, MMTV(SW) and MMTV(SHN), were compared. Finally, the observation of increased frequencies of TcR V beta 2+, V beta 4+ and V beta 8+ CD4+ T cell subsets in Mtv-8+ and Mtv-9+ mice, and TcR V beta 4+ CD4+ T cells in Mtv-6+ and Mtv-7+ mice, when compared with the T cell repertoire of Mtv- mice, is consistent with the possibility that MMTV products contribute to positive selection of T cells.

Água no solo e rendimento do trigo, soja e milho associados ao El Niño Oscilação Sul

O objetivo deste trabalho foi simular o conteúdo de água disponível no solo e o rendimento das culturas de trigo, soja e milho, em Santa Maria, RS, e associá-los ao fenômeno El Niño Oscilação Sul (ENOS). O período estudado foi de 1969 a 2003. A água disponível no solo e o rendimento das culturas foram calculados com modelos matemáticos disponíveis na literatura. A água disponível no solo foi representada pela fração de água transpirável no solo pelas plantas. Foi constatado que a menor disponibilidade hídrica no solo está associada a anos neutros, e a maior disponibilidade hídrica está relacionada a eventos do El Niño. Os anos de La Niña foram os mais favoráveis ao rendimento de grãos da cultura de trigo, enquanto os anos de El Niño foram os mais favoráveis ao rendimento de grãos de soja e milho. Ficou evidente que os anos classificados como neutros, em relação ao ENOS, são os de maior risco de perda de rendimento de grãos destas duas culturas de verão, em conseqüência da menor disponibilidade hídrica no solo, o que é uma informação importante no planejamento de estratégias para o agronegócio relativamente a uma previsão do fenômeno ENOS.