La bioseguridad de las plantas transgénicas

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Interferěncia da comunidade infestante sobre plantas de Eucalyptus grandis de segundo corte

Currently Brazil is one of the leading paper and pulp producers in the world market, where Sao Paulo State boasts the greatest production. Because of the pulp prices falling in the world market and the low costs of a second coppice rotation, two experiments (started May and December, 2000) were conducted to evaluate the effects of weeds and of weed-free periods (0, 3, 6, 9, 12, 15 and 18 months) on the growth of Eucalyptus grandis second coppice plants. The field trials were set up in a randomized block design with four replicates and the experimental plots consisted of three rows of fve plants. The December weed community was composed mainly of Brachiaria decumbens (Surinam grass) and Panicum maximum (Guinea grass) and the May weed community was composed mainly by B. decumbens and Digitaria insularis (Sour-grass). Weeds had a low negative influence on growth, diameter development and macronutrients content of E. grandis second coppice plants. In both experiments, slight reductions in growth were observed only between the fully weeded and weed-free periods, after 18 months.

Efeito da chuva sobre a ação do herbicida imazapyr no controle de plantas daninhas aquáticas

The aim of this study was to evaluate the influence of different periods of rainfall that occurred after the application of the imazapyr herbicide on the control efficiency of Pistia stratiotes and Eichhornia crassipes. We studied 10 mm rainfall, applied for 5' at different time periods (0h, 2h, 4h, 6h, 8h, 12h, 24h, not simulated rainfall) after the application of imazapyr formulation Arsenal NA, 250 g ai ha -1 (1.0 L cp ha -1). The experiment had completely randomized design with four replications. Plants were evaluated at 7, 14, 21, 28 and 35 days after application (DAA), and the results submitted to analysis of variance by F test and treatment means compared by Tukey's test (p <0. 05). The occurrence of rainfall up to 6 hours after herbicide application, plants of P. stratiotes, negatively affected its efficiency and control; rainfall intervals over 8 hours had no effect in control. The occurrence of rainfall at all time intervals did not affect the control of E. crassipes plants.

Influência de laminas de chuva no controle de plantas daninhas, semeadas em diferentes profundidades no solo, com aplicação de herbicidas pré-emergentes

The aim of this work was to evaluate the control in pre-emergence of the weeds brachiaria grass (Brachiaria decumbens) and goosegrass (Eleusine indica) in different depths in the ground, for the oxyfluorfen and isoxaflutole herbicides, applied in pre-emergence condition and submitted of different rain blades. The experimental design was entirely at random, with four repetitions. Each set was consisted of plastic vases with substratum capacity of 4L, filled with arenaceous ground, increased of brachiaria grass and goosegrass seeds in the depths: 0,5; 1,0; 3,0; 6,0 and 9,0 cm. Doses of 37,5 g a.i. ha -1 of isoxaflutole and 720 g a.i. ha -1 of oxyfluorfen were used. The rain blades was 5, 10 and 20 mm applied with a stationary rain simulator, beyond a treatment without rain. Visual evaluations of control at 7 and 14 days had been carried through after application of the herbicides, counting of germinated plants and dry biomass of plants without application. The development of both weeds was inhibited by herbicides, reaching 100% of control, in all depth levels. The different rain blades had not influenced the control of the weeds. In the biggest depth of sowing (9,0 cm), the emergence was above 45% to B. decumbens and 36% to E. indica in treatments without herbicides application.

Utilização de extratos de plantas medicinais e óleo de Eucaliptus no controle in vitro de Microsporum canis

INTRODUCTION: Microsporum canis is the most common cause of canine and feline dermatophytosis and thus has an important zoonotic role. OBJECTIVES: the aim of this study was to determine the antifungal action of medicinal plant extracts and of eucalyptus oil against pathogenic fungus Microsporum canis. METHODS: the extracts were prepared by mixing 300 g of previously washed leaves with 450 mL of distilled water. Then the material was triturated, filtered, sterilized and conserved at 10 + 2 oC. Fifteen milliliters of sterilized medium Sabouraud dextrose (Difco) at a temperature of 55 + 1 oC was added in Petri dishes containing the extracts in one, two, three, four and five mm concentrations. The fungus was inoculated once the medium was solidified. The inoculated dishes were maintained in B.O.D. incubator at 36 ± 0,5 oC until the fungus developed in the controls. RESULTS: the extracts from Punica granatum, Mangifera indica and Eucalyptus spp reduced the growth of fungus, but the extracts from Cymgopogom nardus, Tagetes minuta, Ruta graviolens, Cyperus rotundus, Annona moricata and Calendula spp leaves and flowers boosted the growth of fungus. The other extracts and the eucalyptus oil neither show any fungicidal action nor encourage mycelium growth. CONCLUSIONS: the use of most tested extracts and eucalyptus oil is not suitable for the treatment of Microsporum canis dermatophytosis due to lack of inhibitory effects. The extracts from Cymgopogom nardus, Tagetes minuta, Ruta graviolens, Cyperus rotundus, Annona moricata and from of Calendula spp leaves and flowers help the development of the fungus making clear that phytotherapy should be properly used, otherwise it can worsen the problem. However; extracts from Mangifera indica, Punica granatum and Eucalyptus spp. can be used as fungistatic.

Deficiências de macronutrientes em plantas de soja cv. BRSMG 68 (Vencedora) cultivada em solução nutritive

Soybean is a crop of great importance for Brazil, and knowledge of their nutritional status allows production and high quality products. Thus, the present work aimed to evaluate the culture of the soybean cv. BRSMG 68 (Vencedora), submitted to macronutrients omission on the development, nutritional status and the visual symptomatology of nutrient deficiency. The design used was completely randomized, with seven treatments that corresponded to the complete (macro and micronutrients) solution and the individual omission of N, P, K, Ca, Mg and S. The treatments were applied two weeks after the emergency of the soybean. The nutrient solution was constantly oxygenated and replaced weekly, and the pH monitored daily remained around 5. After six weeks of application of the treatments, the height of the plants, number of leaves, stem diameter was evaluated, matter dry accumulated in the roots, it leaves aerial and the nutritional state and the characterization of the visual symptoms. The macronutrient deficiency promoted a decrease in production of root and shoot dry mass of soybean cultivar BRSMG 68 (Vencedora) in nutrient solution, for the full treatment. The most limiting nutrient for dry matter production of soybean compared to complete treatment were N, S and K, with decreases of 90, 76 and 73%, respectively. Individual omissions resulted in morphological changes, which were translated by characteristic visual symptoms of nutritional deficiency of the respective nutrient.

Período total de prevenção a interferência das plantas daninhas na cultura da alface cultivar Solaris

This research work aimed to determine the total period of interference of weeds prevention of lettuce cultivar Solaris. The experiment was conducted in Universidade Estadual de Londrina, in the period of autumn/ winter of 2008. The lettuce crop remained free from weed interference from emergence up to 0, 10, 20, 30, 40, 50 e 60 days. The experiment was arranged in a randomized block design with four replications. The weed community was composed by Bidens pilosa, Amaranthus hybridus, Oxalis corniculata, Euphorbia heterophylla, Spinacia oleracea, Commelina benghalensis e Lepidium virginicum. It was determined dry and fresh matter of weeds, and number of lettuce leaves. Assuming a tolerance of 5% for crop production decrease, it was verified that the total period of interference prevention (TPIP) occurred up to 21 days after transplanting. Weed interference throughout the crop cycle reduced 25% of its yield.

Influência da cobertura de aveia-preta e milheto sobre comunidade de plantas daninhas e produção de soja

The success in weed control by using straw depends on the management time, straw quality and quantity. The objective of this research was to evaluate the suppressor effect of black-oats and millet straw cover on the incidence of weeds and on the productivity of the soybean crop. The experiment was carried out during the years 2006 and 2007, in an area from the Farm of Education, Research and Production of FCAV-UNESP, Jaboticabal Campus, in a completely randomized block design, with four replications. Treatments consisted of different types of black oats and pearl millet straw management before soybean seeding (without management, obtained by machine cut, desiccated using glyphosate desiccation and with paraquat) and different periods of straw deposition (0, 15, 30 e 60 days before soybean seeding). The weed population and the characteristics of soybean growth and production were evaluated. The deposition of black oats and pearl millet straw provided reductions in weed density and dry mass, and this effect was more effective when the deposition occurred until 15 days prior to soybean seeding. The suppressor effect of black oats straw was more pronounced than that of pearl millet straw, regardless of the drying method, although the millet had a greater dry matter production. No significant differences were found between the drying methods and the straw effectiveness.

Emergência de plantas daninhas em função da posição da semente e quantidade de palha de cana-de- açúcar

This work was carried out to evaluate the effects of sugarcane straw density and positions of seeds of Euphorbia heterophylla, Mucuna cinereum, Digitaria nuda and Eleusine indica on emergence and initial development of plants. The experiment were arranged in a completely randomized design, with 15 treatments and four replications, in a factorial arrangement 3 x 5 with a additional control; the first factor was constituted by position of seeds related to the straw (under, between and on) and the second by five quantities of straw (1, 2, 4, 8 e 16 t ha -1) with a control without straw. At 15 days after sowing (DAS) it was realized evaluations of height, dry mass and emergence percentage for E. heterophylla and M. cinereum, and at 28 DAS it was analyzed dry mass and emergence percentage for D nuda e E. indica. Data obtained were submitted to F in variance analysis test and means were compared with Tukey at 5% probability. Emergence and initial development of E. heterophylla e M. cinereum were reduced with increasing of straw and were affected by seed position, but it was not inhibited in none of conditions, indicating aggressiveness of these weeds in sugarcane mechanized harvest conditions. D. nuda and E. indica emergence was not affected by seed position and coverage soil with 8 e 16 t ha -1 of sugarcane straw inhibited its emergence.

Esterco bovino para o desenvolvimento inicial de plantas provenientes de quatro matrizes de Corymbia citriodora

This work aimed to evaluate the effect of cattle manure levels in the phase of implementation Corymbia citriodora (Hook.) K. D. Hill & L. A. S. Johnson (Eucalyptus citriodora) seedlings in field, produced from seeds collected from four mother trees. The experiment was carried out in green house, in pots filled with 5 dm 3 of Oxisol. Five cattle manure levels were applied 0, 10, 20, 30, and 40 t ha -1 (0, 25, 50, 75, and 100 g pot -1). All treatments received lime levels which were calculated to increase the base saturation degree to 50%. The soil fertility was evaluated after 30 days of soil incubation with manure and lime. The seeds were collected from mother trees called 2, 8, 20 and 29. Sowing was performed directly in rigid plastic containers of 50 cm 3 and seedlings were transplanted when they were around 17 cm height. The experimental design was entirely randomized, in a factorial 5 × 4 (five doses of cattle manure and seeds of four mother trees) scheme and four repetitions. Each parcel was a pot with two plants. At 90 days the height, foliar area, stalk diameter, shoot and root dry matter. The plants responded positively to application of manure, but differently for each evaluated growth characteristic linearly or quadratically. The manure levels had linear effects on growth characteristics and dry matter production of the plants from mother trees 2 and 20 moreover these plants require more cattle manure levels than ones from mother trees 8 and 29. The cattle manure promoted the best development of plants from mother trees 8 and 29, in relationship with dry matter production of shoot components was approximately 27 t ha-1, equivalent to 67.5 g pot.

Taxa de semeadura de Brachiaria brizantha consorciada com milho na incidência de plantas daninhas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fluorescência da clorofila-a e variação da simetria como ferramentas de investigação de plantas sob estresse

Environmental disturbances in various systems and individuals can result in a tendency for fluctuating asymmetry, which consists of a non-directional deviation from symmetry of a bilateral structure in a normally distributed population. Individuals unable to exhibit plastic responses to developmental disorders that occur at random, either genetic or environmental, may exhibit slight deviations from symmetry in organs with bilateral structure such as leaves. Thus analyzing plants by the morphological pattern of symmetry of the leaves has become an effective tool to describe the developmental of plants under stress conditions. This study aimed to analyze if the pattern of symmetry can be used as a tool to investigate the stress level to which a plant is subjected, using ultraviolet-C radiation (UV-C) as source of stress. We determined the leaf symmetry deviations (sd), the average length (AL) and the maximum width of the lamina (MWL) and the fluorescence parameters of chlorophyll-a in leaves of young plants of G. max subjected to 0, 30, 60, 120 or 240 minutes per week of UV-C during four weeks, submitting the values to ANOVA and Tukey test (p <0.05). The results showed significant reductions in AL and MWL, significant increase in sd in leaves irradiated and a drop in potential quantum yield of photosystem II (PSII) and in the photosynthesis performance index (IP). The analysis of leaf symmetry showed high correlation with the stress level to which plants were submitted, being an important analysis to ascertain the fitness of the plant because it is a noninvasive method and easy to perform.

Cambio tecnológico en tres plantas metalmecánicas mexicanas

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Controle da mancha angular do feijoeiro com uso de fungicidas e seu efeito na produção das plantas

The angular leaf spot of dry bean caused by the fungus Phaeoisairopsis griseola is a very important disease on dry bean crops in Brazil. In propitious weather conditions, susceptible cultivars have significant losses due to this disease. In these conditions, fungicide usage is an important step for angular leaf spot management. Since the effects of this method of control on plant physiology are not known, this study had the following objectives: a) Verify the action of fungicide in physiological changes in dry beans (photosynthesis rate and stomatal conductivity); b) evaluate the efficacy of some fungicides for the control of angular leaf spot; and c) examine the effects of these fungicides on other agronomic parameters; weight of 100 grains, number of grains per pod and number of pods per plant. The dry bean cultivar used in this study was Perola. Four trials were conducted, two in a net greenhouse and two trials under field conditions. A spray of piraclostrobin, tebuconazol and tebuconazol+ trifloxistrobin increased photosynthesis rates, although stomatal conductivity was not changed. In addition, sprays of tebuconazol, piraclostrobin, metiram+piraclostrobin and azoxistrobin provided better levels of disease control and also higher weight of 100 grains, with the exception of azoxystrobin for this parameter.

Plantas de cobertura e adubação nitrogenada na cultura do algodoeiro

The fiber quality, productivity and favorable climate are considered key points for the development of the cotton crop in the Brazilian Cerrado. This study aimed to evaluate the effect of cover crops and N application in presowing on the cotton crop growth and yield. The experimental design was randomized blocks, consisting of a - three cover crops (radish, white oat and black oat) implanted during the winter period, b - four nitrogen levels (0, 30, 60 and 90 kg ha-1 of N) applied to the millet residues and before cotton sowing. In April 2008, evaluations were realized of plant development and also harvesting was performed of experimental plots of cotton plants. The results showed that the radish provides increased length of branches and cotton bolls of cotton crop, without yield increase, and the use of increasing doses of N up to 90 kg ha-1 decreased the amount of carimã per plant, increasing the number of reproductive branches and cotton yield.