An indole oxidase isolated from the leaves of Tecoma stans

The presence of an indole oxidase (indole: O2 oxidoreductase) was detected in the leaf extracts of Tecoma stans. The end product of the reaction was identified as anthranil. Formylaminobenzaldehyde, and o- aminobenzaldehyde were detected as intermediates in the overall conversion. Oxygen-uptake studies established that 3 atoms of oxygen were consumed in the formation of anthranil form I molecule of indole. The enzyme showed an absolute requirement for FAD and Cu2+ for maximum activity. FMN was ineffective as a cofactor. The enzyme had an optimum pH of 5.0. Inhibition studies with GSH and p-chloromericuribenzoate showed that a sulfhydrylcupric-ion complex at the active centre is highly essential.

Depression and Cognition in Type 2 Diabetes from a Life Course Perspective

Background: Type 2 diabetes is linked to several complications which add to both physical and mental distress. Depression is a common co-morbidity of diabetes which can occur both as a cause and a consequence of type 2 diabetes. Depression has been shown to correlate with glucose regulation and treating depression might prove beneficial for glucose regulation as well as for mental well being. Another complication which might affect diabetes management is cognitive decline. Several risk factors and complications of diabetes might modify the risk for developing cognitive impairment, which is increased 1.5 times among subjects with type 2 diabetes. Type 2 diabetes, depression and impaired cognitive performance have all been linked to low birth weight. This thesis aimed to explore the effects and interactions of birth weight, depression and cognitive ability in relation to type 2 diabetes from a life course perspective. Subjects and methods: Studies I, II and V were part of the Helsinki Birth Cohort Study. 2003 subjects participated in an extensive clinical examination at an average age of 61 years. A standard glucose tolerance test (OGTT) was performed and depressive symptoms were assessed using the Beck Depression Inventory (BDI). In addition data was obtained from child welfare clinics and national registers. A subset of the cohort (n=1247) also performed a test on cognitive performance (CogState ®) at the average age of 64. Studies III and IV were randomised clinical trials where mildly depressed diabetic subjects were treated with paroxetine or placebo and the effect on metabolic parameters and quality of life was assessed. The first trial included 14 women and lasted 10 weeks, while the second trial included 43 subjects, both men and women, and lasted 6 months. Results: Type 2 diabetes was positively associated with the occurrence of depressive symptoms. Among diabetic subjects 23.6% had depressive symptoms, compared to 16.7% of subjects with normal glucose tolerance (OR = 1.77, p<0.001). Formal mediation analysis revealed that cardiovascular disease (CVD) is likely to act as a mediator in the association. Furthermore, low birth weight was found to modify the association between type 2 diabetes, CVD and depression. The association between BDI score and having type 2 diabetes or CVD was twice as strong in the subgroup with low birth weight (≤ 2500g) compared with the group with birth weight > 2500g (p for interaction 0.058). In the six months long randomised clinical trial (study IV) paroxetine had a transient beneficial effect on glycosylated haemoglobin A1c (GHbA1c) and quality of life when compared to placebo after three months of treatment. In study V we found that subjects with known diabetes had a consistently poorer level of cognitive performance than subjects with normal glucose tolerance in most of the tested cognitive domains. This effect was further amplified among those born with a small birth weight (p for interaction 0.002). Conclusions: Type 2 diabetes is associated with a higher occurrence of depressive symptoms compared to subjects with normal glucose tolerance. This association is especially strong among subjects with CVD and those born with a low birth weight. Treating depressed diabetic subjects with paroxetine has no long term effect on glucose regulation. Physicians should be aware of depression as an important co-morbidity of type 2 diabetes. Both depression and the cognitive decline often seen among diabetic subjects are increased if the subject is born with a low birth weight. Physicians should recognise low birth weight as an additional risk factor and modifier of diabetic complications.

Regulation of cytochrome P-450b/e gene expression by a heme- and phenobarbitone-modulated transcription factor

The cloned DNA fragment of the cytochrome P-450b/e gene containing the upstream region from position -179 through part of the first exon is faithfully transcribed in freeze-thawed rat liver nuclei. Phenobarbitone treatment of the animal strikingly increases this transcription, and the increase is blocked by cycloheximide (protein synthesis inhibitor) or CoCl2 (heme biosynthetic inhibitor) treatment of animals. This picture correlates very well with the reported cytochrome P-450b/e mRNA levels in vivo and run-on transcription rates in vitro under these conditions. The upstream region (from position -179) was assessed for protein binding with nuclear extracts by nitrocellulose filter binding, gel retardation, DNase I treatment ("footprinting"), and Western blot analysis. Phenobarbitone treatment dramatically increases protein binding to the upstream region, an increase once again blocked by cycloheximide or CoCl2 treatments. Addition of heme in vitro to heme-deficient nuclei and nuclear extracts restores the induced levels of transcription and protein binding to the upstream fragment, respectively. Thus, drug-mediated synthesis and heme-modulated binding of a transcription factor(s) appear involved in the transcriptional activation of the cytochrome P-450b/e genes, and an 85-kDa protein may be a major factor in this regard.

Biotransformations of α-terpineol in the rat: Its effects on the liver microsomal cytochrome P-450 system

Alpha-Terpineol (I), a monocyclic monoterpene tertiary alcohol, is widely used in the manufacture of perfumes, cosmetics, soaps and antiseptic agents. It was reported earlier (Horning et al. 1976) that this monoterpene alcohol when administered to humans is hydroxylated to p-menth-l,2,8-triol (II). It is not known whether c~-terpineol also produces other metabolites during its metabolism in the mammalian system and if so, the nature of these metabolites.

Discovery of oxidative enzymes for food engineering : Tyrosinase and sulfhydryl oxidase

Enzymes offer many advantages in industrial processes, such as high specificity, mild treatment conditions and low energy requirements. Therefore, the industry has exploited them in many sectors including food processing. Enzymes can modify food properties by acting on small molecules or on polymers such as carbohydrates or proteins. Crosslinking enzymes such as tyrosinases and sulfhydryl oxidases catalyse the formation of novel covalent bonds between specific residues in proteins and/or peptides, thus forming or modifying the protein network of food. In this study, novel secreted fungal proteins with sequence features typical of tyrosinases and sulfhydryl oxidases were iden-tified through a genome mining study. Representatives of both of these enzyme families were selected for heterologous produc-tion in the filamentous fungus Trichoderma reesei and biochemical characterisation. Firstly, a novel family of putative tyrosinases carrying a shorter sequence than the previously characterised tyrosinases was discovered. These proteins lacked the whole linker and C-terminal domain that possibly play a role in cofactor incorporation, folding or protein activity. One of these proteins, AoCO4 from Aspergillus oryzae, was produced in T. reesei with a production level of about 1.5 g/l. The enzyme AoCO4 was correctly folded and bound the copper cofactors with a type-3 copper centre. However, the enzyme had only a low level of activity with the phenolic substrates tested. Highest activity was obtained with 4-tert-butylcatechol. Since tyrosine was not a substrate for AoCO4, the enzyme was classified as catechol oxidase. Secondly, the genome analysis for secreted proteins with sequence features typical of flavin-dependent sulfhydryl oxidases pinpointed two previously uncharacterised proteins AoSOX1 and AoSOX2 from A. oryzae. These two novel sulfhydryl oxidases were produced in T. reesei with production levels of 70 and 180 mg/l, respectively, in shake flask cultivations. AoSOX1 and AoSOX2 were FAD-dependent enzymes with a dimeric tertiary structure and they both showed activity on small sulfhydryl compounds such as glutathione and dithiothreitol, and were drastically inhibited by zinc sulphate. AoSOX2 showed good stabil-ity to thermal and chemical denaturation, being superior to AoSOX1 in this respect. Thirdly, the suitability of AoSOX1 as a possible baking improver was elucidated. The effect of AoSOX1, alone and in combi-nation with the widely used improver ascorbic acid was tested on yeasted wheat dough, both fresh and frozen, and on fresh water-flour dough. In all cases, AoSOX1 had no effect on the fermentation properties of fresh yeasted dough. AoSOX1 nega-tively affected the fermentation properties of frozen doughs and accelerated the damaging effects of the frozen storage, i.e. giving a softer dough with poorer gas retention abilities than the control. In combination with ascorbic acid, AoSOX1 gave harder doughs. In accordance, rheological studies in yeast-free dough showed that the presence of only AoSOX1 resulted in weaker and more extensible dough whereas a dough with opposite properties was obtained if ascorbic acid was also used. Doughs containing ascorbic acid and increasing amounts of AoSOX1 were harder in a dose-dependent manner. Sulfhydryl oxidase AoSOX1 had an enhancing effect on the dough hardening mechanism of ascorbic acid. This was ascribed mainly to the produc-tion of hydrogen peroxide in the SOX reaction which is able to convert the ascorbic acid to the actual improver dehydroascorbic acid. In addition, AoSOX1 could possibly oxidise the free glutathione in the dough and thus prevent the loss of dough strength caused by the spontaneous reduction of the disulfide bonds constituting the dough protein network. Sulfhydryl oxidase AoSOX1 is therefore able to enhance the action of ascorbic acid in wheat dough and could potentially be applied in wheat dough baking.

Att äta eller inte äta, att äta nu eller senare. En studie i ätstörningar ur ett samhällsvetenskapligt perspektiv

Tiivistelmä – Referat – Abstract Anorexi ses i Finland som en psykisk sjukdom med en psykopatologi, som bland andra innefattar rädsla för att bli fet. I min undersökning har jag fått fram tre huvudteman, som förutom andra bakomliggande omständigheter, bidragit till ett insjuknande. De handlar om anorexi som livsstil, bantning och viljan att leva sunt och äta rätt. Med den vaknande sexualiteten väcks intresset för det andra könet och för kroppens utseende i övrigt. I bakgrunden finns känslor av bl.a. ensamhet, ett sökande efter den egna personligheten, ångest och depression. Jag har byggt min studie på patienternas egna berättelser om sitt liv. Perspektivet är samhällsinriktat, och som analysmetod har jag använt mig av narrativet. Jag har lånat idéer både från Vilma Hänninens (1999), Gerhard Riemann och Fritz Schützes (1991) narrativa modeller, och rör mig mellan dem båda. Jag har i första hand intresserat mig för tiden före insjuknandet och de bakomliggande orsaker, som kan tänkas befrämja insjuknandet. Jag har speglat anorektikernas problematik i bl.a. Anthony Giddens (1997) och Zygmund Baumans (1999) teorier om det postmoderna samhället, och ser ätstörningar som en uttrycksform för dåligt befinnande. Samhällsklimatet har blivit hårdare och omsorgen om personer i omgivningen har glömts bort. Vi lever, enligt Giddens (1997), i en skenande värld, viket betyder att förändringarna påverkar existerande beteendemönster i större omfattning och på ett djupare plan än tidigare. Enligt Pennanen (2000) är målsättningen för ätstörningspatienter stressande, svåruppnådd och svårkontrollerbar. Mina informanter berättar om höga målsättningar, prestationskrav, dålig självkänsla och skam över sina kroppar. För att tillfriskna behövs stöd. Men det vikigaste är att personen själv vill bli frisk, och själv ta ansvar för sitt tillfrisknande. Sjukdomen är individuell. Varje person, upplever sin sjukdom personligt men anorektikerna har också många styrkor i sin sjukdom. Avainsanat – Nyckelord – Keywords ätstörningar, anorexi, bulimi

Att äta eller inte äta, att äta nu eller senare. En studie i ätstörningar ur ett samhällsvetenskapligt perspektiv

Anorexi ses i Finland som en psykisk sjukdom med en psykopatologi, som bland andra innefattar rädsla för att bli fet. I min undersökning har jag fått fram tre huvudteman, som förutom andra bakomliggande omständigheter, bidragit till ett insjuknande. De handlar om anorexi som livsstil, bantning och viljan att leva sunt och äta rätt. Med den vaknande sexualiteten väcks intresset för det andra könet och för kroppens utseende i övrigt. I bakgrunden finns känslor av bl.a. ensamhet, ett sökande efter den egna personligheten, ångest och depression. Jag har byggt min studie på patienternas egna berättelser om sitt liv. Perspektivet är samhällsinriktat, och som analysmetod har jag använt mig av narrativet. Jag har lånat idéer både från Vilma Hänninens (1999), Gerhard Riemann och Fritz Schützes (1991) narrativa modeller, och rör mig mellan dem båda. Jag har i första hand intresserat mig för tiden före insjuknandet och de bakomliggande orsaker, som kan tänkas befrämja insjuknandet. Jag har speglat anorektikernas problematik i bl.a. Anthony Giddens (1997) och Zygmund Baumans (1999) teorier om det postmoderna samhället, och ser ätstörningar som en uttrycksform för dåligt befinnande. Samhällsklimatet har blivit hårdare och omsorgen om personer i omgivningen har glömts bort. Vi lever, enligt Giddens (1997), i en skenande värld, viket betyder att förändringarna påverkar existerande beteendemönster i större omfattning och på ett djupare plan än tidigare. Enligt Pennanen (2000) är målsättningen för ätstörningspatienter stressande, svåruppnådd och svårkontrollerbar. Mina informanter berättar om höga målsättningar, prestationskrav, dålig självkänsla och skam över sina kroppar. För att tillfriskna behövs stöd. Men det vikigaste är att personen själv vill bli frisk, och själv ta ansvar för sitt tillfrisknande. Sjukdomen är individuell. Varje person, upplever sin sjukdom personligt men anorektikerna har också många styrkor i sin sjukdom.

Competing peroxidase and oxidase reactions in scopoletin-dependent H2O2-initiated oxidation of NADH by horseradish peroxidase

Addition of NADH inhibited the peroxidative loss of scopoletin in presence of horseradish peroxidase and H2O2 and decreased the ratio of scopoletin (consumed):H2O2 (added). Concomitantly NADH was oxidized and oxygen was consumed with a stoichiometry of NADH: O-2 of 2:1. On step-wise addition of a small concentration of H2O2 a high rate of NADH oxidation was obtained for a progressively decreasing time period followed by termination of the reaction with NADH:H2O2 ratio decreasing from about 40 to 10. The rate of NADH oxidation increased linearly with increase in scopoletin concentration. Other phenolic compounds including p-coumarate also supported this reaction to a variable degree. A 418-nm absorbing compound;d accumulated during oxidation of NADH. The effectiveness of a small concentration of H2O2 in supporting NADH oxidation increased in presence of SOD and decreased in presence of cytochrome c, but the reaction terminated even in their presence. The results indicate that the peroxidase is not continuously generating H2O2 during scopolerin-mediated NADH oxidation and that both peroxidase and oxidase reactions occur simultaneously competing for an active form of the enzyme.

Anthropogenic aerosol fraction over Afro-Asian regions inferred using Kalpana-I and MISR data

Mineral dust constitutes the single largest contributor of natural aerosols over continents. The first step towards separating natural aerosol radiative impact from its anthropogenic counterparts over continents is to gather information on dust aerosols. The infrared (IR) radiance (10.5-12.5 mu m) acquired from the Kalpana-I satellite (similar to 8-km resolution) was used to retrieve regional characteristics of dust aerosols over the Afro-Asian region during the winter of 2004, coinciding with a national aerosol campaign. Here, we used aerosol-induced IR radiance depression as an index of dust load. The regional distribution of dust over various arid and semi-arid regions of India and adjacent continents has been estimated, and these data in conjunction with regional maps of column aerosol optical depth (AOD) are used to infer anthropogenic aerosol fraction. Surprisingly, even over desert locations in India and Saudi Arabia, the anthropogenic fraction was relatively high (similar to 0.3 to 0.4) and the regionally averaged anthropogenic fraction over India was 0.62 +/- 0.06.

Aquaculture Asia, Vol.13, No.2, pp.1-56, April-June 2008

Peter Edwards writes on rural aquaculture: From integrated carp polyculture to intensive monoculture in the Pearl River Delta, South China. Better management practices for Vietnamese catfish. Ipomoea aquatica – an aquaculture friendly macrophyte. A status overview of fisheries and aquaculture development in Pakistan with context to other Asian countries. The changing face of post-grad education in aquaculture: contributing to soaring production and sustainable practices. Hatchery management in Bangladesh. Production of Cirrhinus molitorella and Labeo chrysophekadion for culture based fisheries development in Lao PDR Part I: Captive spawning. Application of ipil-ipil leaf meal as feed Ingredient for monosex tilapia fry (Oreochromis niloticus) in terms of growth and economics. Fermented feed ingredients as fish meal replacer in aquafeed production Aquaculture and fishing management in coastal zone demarcation: the case of Thailand. Reservoir fisheries of freshwater prawn – success story of an emerging culture-based giant freshwater prawn fishery at Malampuzha Dam in Kerala, India. Determining and locating sea cage production area for sustainable tropical aquaculture. SPC Pacific-Asia marine fish mariculture technical workshop: “Farming Marine Fishes for our Future”. Developing Better Management Practices for Marine Finfish Aquaculture. Breeding and seed production of silver pompano (Trachinotus blochii, Lacepede) at the Mariculture Development Center of Batam. Potential of silver pomfret (Pampus argenteus) as a new candidate species for aquaculture. NACA Newsletter.

Aquaculture Asia, Vol. 10, No. 4, pp.1-42, October-December 2005

*Table of Contents* Sustainable aquaculture Peter Edwards writes on rural aquaculture: Small-scale pond culture in Bangladesh. People in aquaculture Community based aquaculture - issues and challenges H.K. De and G.S. Saha. Aquaculture as an action programme: An exercise in building confidence and self worth. B. Shanthi, V.S. Chandrasekaran, M. Kailasam, M. Muralidar, T. Ravisankar,.C. Saradad and M. Krishnan The STREAM Column: Transforming policy recommendations into pro-poor service provision Graham Haylor. Research & farming techniques. Grow out of juvenile spotted Babylon to marketable size in earthen ponds II: Polyculture with seabass. S. Kritsanapuntu, N. Chaitanawisuti, W. Santhaweesuk and Y. Natsukari Asia-Pacific Marine Finfish Aquaculture Network. Influence of economic conditions of importing nations and unforeseen global events on grouper markets. Sih Yang Sim. Present status of hatchery technology for cobia in Vietnam. Nhu Van Can. Report on grouper hatchery training course in Indonesia. Nguyen Quoc Thai. Aquatic animal health. Biosecured and improved penaeid shrimp production through organic nursery raceway system in India. Felix. S. and M. Samaya Kannan. Management of monogenean parasites in brackishwater finfish. K.P. Jithendran, M. Natarajan and I.S. Azad. Vembanad Lake: A potential spawner bank of the giant freshwater prawn Macrobrachium rosenbergii on the southwest coast of India. Paramaraj Balamurugan, Pitchaimuthu Mariappan & Chellam Balasundaram.

Strategies and options for increasing and sustaining fisheries and aquaculture production to benefit poorer households in Asia

The last three decades have witnessed dramatic changes in the structure of supply and demand for fish, especially in Asia. This WorldFish research study sponsored by the Asian Development Bank focussed on nine developing countries – Bangladesh, China, India, Indonesia, Malaysia, the Philippines, Sri Lanka, Thailand, and Vietnam, all active players in the transformation of global fish supply and demand. The study, broken into five components and reported here, considered: 1) the profile of key aquaculture technologies and fishing practices; 2) analysis of policies, institutions and support services; 3) socioeconomic profile of major stakeholders in the fisheries sector; 4) projections of fish demand and supply in the nine Asian countries; and 5) formulation of national action plans based on the findings and recommendations of the study.

Ecology of aquaculture species and enhancement of stocks: proceedings of the thirtieth U.S. - Japan meeting on aquaculture, Sarasota, FL, Dec. 3-4, 2001

CONTENTS: I. U.S.-Japan Cooperation Open Ocean Aquaculture – A Venue for Cooperative Research Between the United States and Japan.............................................................................. 1 C. Helsley II. Growth, Nutrition and Genetic Diversity Daily Ration of Hatchery-Reared Japanese Flounder Paralichthys olivaceus as an Indicator of Release Place, Time and Fry Quality. In situ Direct Estimation and Possibility of New Methods by Stable Isotope............................ 7 O. Tominaga, T. Seikai, T. Tsusaki, Y. Hondo, N. Murakami, K. Nogami, Y. Tanaka and M. Tanaka Nucleic Acids and Protein Content as a Measure to Evaluate the Nutritional Condition of Japanese Flounder Paralichthys olivaceus Larvae and Juveniles........................................................................................................ 25 W. Gwak Genetic Diversity Within and Between Hatchery Strains of Flounder Paralichthys olivaceus Assessed by Means of Microsatellite and Mitochondrial DNA Sequencing Analysis...................................................................... 43 M. Sekino, M. Hara and N. Taniguchi Tracking Released Japanese Flounder Paralichthys olivaceus by Mitochondrial DNA Sequencing................................................................................ 51 T. Fujii Preliminary Aspects of Genetic Management for Pacific Threadfin Polydactylus sexfilis Stock Enhancement Research in Hawaii........................................ 55 M. Tringali, D. Ziemann and K. Stuck Enhancement of Pacific Threadfin Polydactylus sexfilis in Hawaii: Interactions Between Aquaculture and Fisheries............................................................. 75 D. Ziemann Aquaculture and Genetic Structure in the Japanese Eel Anguilla japonica..................... 87 M. Katoh and M. Kobayashi Comparative Diets and Growth of Two Scombrid Species, Chub Mackerel Scomber japonicus and Japanese Spanish Mackerel Scomberomorus niphonius, in the Central Seto Inland Sea, Japan.................................. 93 J. Shoji, M. Tanaka and Tsutomu Maehara iii Evaluating Stock Enhancement Strategies: A Multi-disciplinary Approach................... 105 T. M. Bert, R.H. McMichael, Jr., R.P. Cody, A. B. Forstchen, W. G. Halstead, K. M. Leber, J. O’Hop, C. L. Neidig, J. M. Ransier, M. D. Tringali, B. L. Winner and F. S. Kennedy III. Physiological and Ecological Applications Predation on Juvenile Chum Salmon Oncorhynchus keta by Fishes and Birds in Rivers and Coastal Oceanic Waters of Japan................................... 127 K. Nagasawa and H. Kawamura Interaction Between Cleaner and Host: The Black Porgy Cleaning Behavior of Juvenile Sharpnose Tigerfish Rhyncopelates Oxyrhynchus in the Seto Inland Sea, Western Japan............................................................................. 139 T. Shigeta, H. Usuki and K. Gushima IV. Case Studies Alaska Salmon Enhancement: A Successful Program for Hatchery and Wild Stocks............................................................................................... 149 W. Heard NMFS Involvement with Stock Enhancement as a Management Tool........................... 171 T. McIlwain Stock Enhancement Research with Anadromous and Marine Fishes in South Carolina...................................................................................... 175 T. I. J. Smith, W. E. Jenkins, M. R. Denson and M. R. Collins Comparison of Some Developmental, Nutritional, Behavioral and Health Factors Relevant to Stocking of Striped Mullet, (Mugilidae), Sheepshead (Sparidae), Common Snook (Centropomidae) and Nassau Groupers (Serranidae)........................... 191 J. W. Tucker Jr. and S. B. Kennedy Participants in the Thirtieth U.S.-Japan Meeting on Aquaculture................. Inside Back Cover iv (PDF has 204 pages.)

Livelihood analysis and development of a planning support for the poor in aquaculture and aquatic resource management. Workshop proceedings. 13-15 February 2001, Thai Nguyen, Vietnam

The workshop was organized to understand and describe the livelihoods of poor people who manage aquatic resources for planning support. The purpose was to support field workers to carry out livelihood analysis and how to use this information. (PDF contains 13 pages)

Symposium on participatory approaches to reservoir fisheries management: issues, challenges and policies. Dambulla, Sri Lanka, 04-06 Oct. 2004. Session I: Community-based fisheries management: experience in other countries

This paper advocates strategies, processes and practices that enable: livelihoods approaches rather than resource-based approaches, ‘direct’ institutional and policy development, rather than ‘project demonstrations’, and support for regional, national and local communications. (Pdf contains 12 pages).