Morfofisiologia da cunhã cultivada sob estresse salino

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Germinação de sementes de romäzeiras (Punica granatum L.) de acordo com a concentração de giberelina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crescimento inicial e absorção de zinco pelo milho em função do modo de aplicação e fontes do nutriente

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Soil phosphorus availability and soybean response to phosphorus starter fertilizer

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Reação de aceroleira (Malpighia emarginata D.C.) à Meloidogyne enterolobii

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Carbon isotope fractionation for cotton genotype selection

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Anatomização de pinos de fibra de vidro: estudo de propriedades mecânicas na interface restauradora adesiva

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Interação fungo micorrízico arbuscular e adubação fosfática no crescimento e absorção de nutrientes pelo porta-enxerto limoeiro Cravo

Estudou-se o efeito da inoculação com fungo micorrízico arbuscular (FMA), Glomus etunicatum, Glomus clarum, ou Scutelospora heterogama, e da adição de fósforo solúvel (15, 30, 45, 60, 120 e 240 mg de P kg-1 de terra) sobre as variáveis altura, número de folhas, diâmetro do caule, massas vegetais aérea e radicular, teores de nutrientes nas folhas e colonização micorrízica no porta-enxerto limoeiro Cravo (Citrus limonia (L.) Osbeck). Os FMAs, Glomus etunicatum e Glomus clarum, e doses crescentes de fósforo exerceram efeitos significativos sobre essas variáveis. O efeito da inoculação sobre as variáveis de crescimento vegetal foi mais acentuado nos tratamentos com adição de 30 a 60 mg de P kg-1 de terra. Para o teor de P foliar, o efeito da inoculação foi mais acentuado nos tratamentos adicionados de 120 a 240 mg de P kg-1 de terra. A inoculação com estes FMAs aumentou a eficiência do limoeiro Cravo em absorver nutrientes, tanto que as variáveis estudadas em plantas inoculadas na ausência de adubação fosfática superaram às de plantas não inoculadas em substrato adicionado de 240 mg de P kg-1 de terra.

Eficiência de detergentes e EDTA na remoção da smear layer de superfícies radiculares submetidas a raspagem e aplainamento: análise através da microscopia eletrônica de varredura

A remoção da smear layer é um procedimento importante para a regeneração periodontal e para o tratamento da sensibilidade dentinária. Diversos tratamentos tem sido realizados para sua remoção, porém, mostrando resultados conflitantes ou insatisfatórios. No presente estudo nos propomos a analisar através do microscópio eletrônico de varredura a eficiência dos detergentes: lauril sulfato de sódio, Plax e de mamona assim como o EDTA 24% na remoção da smear layer. Todos os produtos testados foram aplicados com bolinha de algodão, renovada a cada 30 segundos, a qual era esfregada na superfície radicular por diferentes períodos. As fotomicrografias foram analisadas através da aplicação de um índice de remoção da smear layer por três examinadores calibrados e os resultados analisados estatisticamente. De acordo com nossos resultados pudemos chegar as seguintes conclusões: 1- a instrumentação com curetas de Gracey provocou a formação da smear layer; 2- o detergente lauril sulfato de sódio não removeu a smear layer, tendo sido o pior resultado entre os detergentes testados; 3- Os detergentes Plax e de mamona mostraram resultados semelhantes entre si e superiores ao controle negativo e ao lauril sulfato de sódio, porém, não removeram a smear layer satisfatoriamente; 4- O EDTA 24% neutro mostrou ser eficiente para a remoção da smear layer.

Estudo biométrico das furcas radiculares vestibular, mesial e distal dos primeiros e segundos molares superiores permanentes de humanos

Foram analisados 100 dentes primeiros molares e 100 dentes segundos molares superiores, direitos e esquerdos, permanentes, extraídos de humanos, com o objetivo de explorar a região anatômica da furca, mais especificamente realizar medidas das distâncias das aberturas das furcas vestibular, mesial, e distal até à junção esmalte-cemento, e das distâncias das referidas aberturas até às faces radiculares pertinentes: vestibular, mesial, distal e lingual. Utilizou-se para mensuração um microcomputador 486 DX-66Mhz, no qual estava instalado um software analisador de imagens Mocha-Jandel Scientific SigmaScan Pro, version 2.0. Em relação às distâncias das aberturas das furcas até à junção esmalte-cemento foi observado que as distâncias médias para a furca vestibular foram de 3,50mm e 3,03mm; para a furca mesial de 4,44mm e 4,69mm; para a furca distal de 4,26mm e 3,73mm, respectivamente para os primeiros e segundos molares superiores. Em relação às distâncias das aberturas das furcas até às faces radiculares pertinentes foi observado que as distâncias médias da furca vestibular até à face mesial da raiz mesio-vestibular foram de 3,78mm e 3,72mm; da furca vestibular até a face distal da raiz disto-vestibular 3,62 e 3,64mm; da furca mesial até à face vestibular da raiz mesio-vestibular 7,33mm e 6,89mm; da furca mesial até a face lingual da raiz lingual de 4,13mm e 4,33mm; da furca distal até à face vestibular da raiz disto-vestibular de 5,61mm e 5,09mm; da furca distal até a face lingual da raiz lingual de 4,73mm e 4,65mm, respectivamente para os primeiros e segundos molares superiores.

Spontaneous osteonecrosis of the jaws in the maxilla of mice on antiresorptive treatment: A novel ONJ mouse model

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Hard and soft tissue changes around implants installed in regular-sized and reduced alveolar bony ridges. An experimental study in dogs

Objective: To study bony and soft tissue changes at implants installed in alveolar bony ridges of different widths.Material and methods: In 6 Labrador dogs, the mandibular premolars and first molars were extracted, and a buccal defect was created in the left side at the third and fourth premolars by removing the buccal bone and the inter-radicular and interdental septa. Three months after tooth extraction, full-thickness mucoperiosteal flaps were elevated, and implants were installed, two at the reduced (test) and two at the regular-sized ridges (control). Narrow or wide abutments were affixed to the implants. After 3 months, biopsies were harvested, and ground sections prepared for histological evaluation.Results: A higher vertical buccal bony crest resorption was found at the test (1.5 +/- 0.7 mm and 1.0 +/- 0.7 mm) compared to the control implants (1.0 +/- 0.5 mm and 0.7 +/- 0.4 mm), for both wide and narrow abutment sites. A higher horizontal alveolar resorption was identified at the control compared to the test implants. The difference was significant for narrow abutment sites. The peri-implant mucosa was more coronally positioned at the narrow abutment, in the test sites, while for the control sites, the mucosal adaptation was more coronal at the wide abutment sites. These differences, however, did not reach statistical significance.Conclusions: Implants installed in regular-sized alveolar ridges had a higher horizontal, but a lower vertical buccal bony crest resorption compared to implants installed in reduced alveolar ridges. Narrow abutments in reduced ridges as well as wide abutments in regular-sized ridges yielded less soft tissue recession compared to their counterparts.

Spontaneous healing responses detected by cone-beam computed tomography of horizontal root fractures: a report of two cases

Horizontal root fractures (HRF) usually affect the anterior teeth as a result of trauma, and generally heal spontaneously, depending on the vitality of the pulp. Diagnosis based on clinical findings, sensitivity tests, and radiographic examination is important to determine the presence of a root fracture and to prevent a root fracture from passing unnoticed. Cone-beam computed tomography (CBCT) has been used successfully for diagnosis and prognosis imaging of root fractures and has proved to be superior to other radiographic methods. This study reports two cases of dental trauma caused by a collision and a sports accident. The patients suffered horizontal root fractures in the maxillary left central incisor and in the mandibular left central incisor. The diagnosis of root fracture was confirmed by cone-beam computed tomography (CBCT) images, which also demonstrated spontaneous healing of the fracture line. The repair occurred by interposition of connective tissue in the former case and by interposition of bone and connective tissue in the latter case. The final diagnoses of both cases were based on CBCT images, indicating the importance of a CBCT examination to reach a firm diagnosis and to follow the healing process of root fracture cases, avoiding unnecessary radical endodontic treatment.

ORGANIC RESIDUE AND PHOSPHORUS AS CONDITIONERS OF DEGRADED SOIL AND EFFECTS ON THE INITIAL GROWTH OF Dipteryx alata Vog.

In northwestern Sao Paulo State, it was established, in 1965, the hydroelectric station of Ilha Solteira (CESP) that produced extensive degraded areas, from where it was removed the soil superficial layers. For the re-establishment of the vegetation, in areas like these, it is necessary the addition of chemical inputs and organic material. Associating the presence in the area of macrophytes, to the need of recovery of degraded areas and the availability of seeds from native species of Cerrado, it was intended the development of this work, with the objective of evaluating the addition effects of organic residue and phosphorus on the material of degraded soil and on the initial growth of Dipteryx alata, arboreal species of 'cerrado'. The soil material was collected in area of degraded soil by the construction of Ilha Solteira hydroelectric station. The experiment was conducted in two environmental conditions, greenhouse and plenty of sun. The treatments consisted of four phosphorus doses (0, 100, 200 and 300 mg dm(-3) of P2O5), with or without macrophytes addition, with 16 treatments and 10 repetitions each one. The used macrophytes, collected at Jupia hydroelectric station, in Mato Grosso do Sul state, were dried, sliced, incorporated to the soil material and incubated during 60 days. The seeds of Dipteryx alata, collected around of the area, were sowed in washed sand and after 60 days introduced in the treatments. The plants were appraised monthly for height, stem diameter and leaves chlorophyll. After 210 days, the plants were collected and evaluated for fresh and dry weight of aerial part and radicular system and the soil material was evaluated for fertility and microbial activity by the carbon of liberated CO2. The organic residue, incorporated to the material of soil degraded, positively affects the soil fertility and the soil microbial activity. The applied phosphorus doses influenced the behavior of Ca, SB, H+Al and CTC, when applied in the absence of organic residue, it promoted significant and linear increments for values of potential acidity, and indicated the organic residue importance. The initial growth of the Dipteryx alata was favored by the protected cultivation, for the incorporation of organic residue and for the phosphorus addition to the soil. At the end although the material of degraded soil comes as good perspective for disposal of this residue, studies about the field conditions are necessary.

Caracterização molecular do gene que codifica a TCTP (Translationally Controlled Tumor Protein) em tomate (Solanum lycopersicum cv. Santa Clara)

The gene encoding TCTP (Translationally Controlled Tumour Protein) is present in all eukaryotes and its product is involved in various cellular processes. Although well characterized in mammals, there are only few works available in the literature related to the analysis of this protein in plants. In this present work, the expression of the gene encoding TCTP was analyzed in different organs/tissues of tomato plants (Solanum lycopersicum cv. Santa Clara). A quantification performed by RT-qPCR revealed the presence of TCTP transcript in all tissues/organs analyzed, with the highest expression level found in leaves. With the exception of fruits in intermediate stage of maturation, for which a small increase on the expression was detected, there was minimal variation in the relative expression of TCTP in other organ/tissues. In parallel, the effects of the constitutive expression of TCTP were investigated using transgenic tobacco lines able to overexpress this protein at different levels (T1, T2 and T3). Seedlings of these lines, and of a non-transgenic control line, were grown in MS culture medium for 21 days. At the end of this period, the length of roots and leaves was taken and the seedlings were photographed. According to Tukey's test, the analysis of the mean root length revealed a significant difference between T1 and T3 lines when compared to the control, although the same was not observed for the T2 line. For leaves, according to Kruskal-Wallis test, there was a statistical difference between the averages of leaf growth obtained for the different lines evaluated. According to these results, we can conclude that TCTP shows an ubiquitous expression in tomato plants, with the highest expression detected in leaves, and also that its overexpression promoted a higher root and leaf development in two of three transgenic tobacco lines tested