Pharmacological evidence for β2-adrenoceptor in right atria from stressed female rats

The purpose of the present study was to demonstrate a physiological response to TA2005, a potent β2-adrenoceptor (β2-AR) selective agonist, in right atria isolated from stressed female rats under the influence of the estrous cycle. We obtained concentration-response curves to the agonist in the presence and in the absence of selective antagonists in right atria isolated from female rats submitted to three daily foot-shock sessions (30 min duration, 120 pulses of 1.0 mA, 1.0 s, applied at random intervals of 5- 25 s) and sacrificed at estrus or diestrus. Our results showed that the pD2 values of TA2005 were not influenced by estrous cycle phase or foot-shock stress. However, in right atria from stressed rats sacrificed during diestins, the concentration-response curve to TA2005 was biphasic, with a response being obtained at concentrations of 0.1 nM, whereas during estrus no response was observed at doses lower than 3 nM. ICI118,551, a β2-AR antagonist, abolished the response to nanomolar concentrations of TA2005 in right atria from stressed rats at diestrus, with no changes in agonist pD2 values in right atria from control rats (7.47 ± 0.09, p > 0.05) but a 3-fold decrease in pD2 values of TA2005 in right atria from foot shock stressed rats (7.90 ± 0.07, p ≤ 0.05). Concentration-response curves to TA2005 in the presence of ICI118,551 were best fitted by a one-site model equation. The β1-AR antagonist, CGP20712A, shifted to the right only the second part of the concentration-response curves to the agonist, unmasking the putative β2-AR-mediated response to the agonist in tissues isolated from stressed rats at diestrus. Under this condition, concentration-response curves to the agonist were best fitted by a two-site model equation, pD2 and maximum response of TA2005 interaction with β1- and putative β2-adrenoceptor components were calculated. Schild analyses gave a pK(B) value for CGP20712A that was typical for the interaction with β1-AR in each experimental group, pK(B) values for ICI118,551 could not be obtained in stressed rats sacrificed at diestins since Schild plot slopes were lower than 1.0. In right atria from control rats, ICI118,551 pK(B) values were similar to reported values for the interaction of the antagonist with β1-AR. These results confirm that a heterogenous β1-AR population mediating the chronotropic response to catecholamines can be demonstrated in right atria from foot shock stressed female rats sacrificed at diestins. The stress-induced response seems to be mediated by the β2-AR subtype. Right atria from rats sacrificed during estrus are protected against stress-induced alterations on the homogeneity of β-AR population.

Key to the nests of Brazilian Epiponini wasps (Vespidae: Polistinae)

Brazil possesses the richest diversity of Epiponini wasps in the world. However, field identification of genera of these wasps, based on morphological features, is difficult without optical equipment. Therefore, this work presents a key to the Brazilian Epiponini genera based on the structural features of the nests.

Dynamics of handling time and functional response by larvae of Chrysomya albiceps (Dipt., Calliphoridae) on different prey species

One way to understand the behavioural patterns exhibited by a predator in response to prey density is to evaluate its functional response. Such evaluation yields information about basic mechanisms of prey-predator dynamics, and is an essential component of prey-predator models. In this paper we analysed experimentally the functional response and the handling time spent by Chrysomya albiceps on different prey species and larval instars of blowflies. The type II functional response was observed when second instar larvae of Chrysomya megacephala and Chrysomya macellaria were consumed. The handling time spent by the predator was significantly different between instars and species. The implications of the functional response and handling time for the interaction dynamics of Brazilian Chrysomyinae species are discussed.

Electromyographic validation of the pectoralis major and deltoideus anterior muscles in inverted fyling exercises with loads

Inverted flying exercise with external loads of 25, 50, 75 and 100% of each individual maximum load in the pectoralis major and deltoideus anterior muscles was electromyographically analyzed in eleven male volunteers, using surface electrodes MEDI-TRACE-200 connected to a biological signals acquisition module coupled to a PC/AT computer. Electromyographic signals were processed and the effective values obtained were standardized through maximum voluntary isometric contraction. When the concentric phase of each muscle with the same load was statistically compared with the eccentric phase, it was observed that for all loads all the muscles presented significant electromyographic difference, and that the concentric phase was always higher. By analyzing the different loads for each muscle, it was noticed that in the concentric phase all the muscles presented significant electromyographic activity, being it higher with maximum load. When the effect of each load on different muscle in the concentric and eccentric phases was analyzed, the muscles presented a distinct activity profile.

Efeito da calagem e sulfato de amônio no algodão. I - Transporte de cátions e ânions no solo

Applying lime on the soil surface in soils managed under no-tillage has caused an excess of basic cations in the most superficial layers of the soil profile. On the other hand, subsoil acidity is considered a constraint to the development of deep plant roots. The objective of this study was to evaluate Ca 2+, Mg 2+, NO 3- and SO 4 2- leaching in the soil profile as affected by liming and top dressing nitrogen fertilization in cotton, grown with straw cover on the soil surface. Cotton plants (Gossypium hirsutum) were grown for 60 days in PVC columns filled with a Distroferric Red Latosol (sand loam Rhodic Oxisol) with liming applied over the straw on the soil surface, incorporated liming 0-20 cm deep, or without liming. Nitrogen was applied at rates of 0, 50, 100 and 150 kg ha -1 as ammonium sulfate. The PVC columns were set up in layers of 0-5, 5-10, 10-20, 20-30 and 30-30 cm, totaling 15.71 dm 3. The ammonium sulfate application caused intense leaching of SO 4 2- in the soil, irrespective of the lime application method. Liming increased the concentration of NO 3 in the 0-20 cm soil layer, whereas the correction of the soil acidity did not affect the NO 3- concentration in the 30-50 cm soil layer. The influence of ammonium sulfate on Ca 2+ leaching below 20 cm was only observed in the soil with incorporated lime. Nitrogen application resulted in extensive Mg 2+ leaching from the soil, regardless of the lime application method. In the soil layer below 30 cm, SO 4 2- presented a higher correlation than NO 3- in the formation of ionic pairs with Ca 2+ and Mg 2+.

Influência das propriedades de granulados de celulose nas características físicas dos comprimidos

The compaction behavior of powdered solids can be strongly influenced by the physicochemical properties of excipients because they are frequently present in the tablet in larger amounts than the drug itself. The aim of this study was to assess the influence of the granule size of the cellulose on the physical characteristics of tablets produced in punches of different diameters, since this relation has never been explored in the literature. Granules of several sizes were produced by wet granulation and compressed in punches of various diameters by applying different forces. Size distribution, apparent density and flow of granules were assessed, as well as physical characteristics of the tablets (weight, hardness, friability and disintegration time). Reducing the granule size resulted in tablets of adequate crushing strength and fast disintegration; moreover, it allowed tablets to be produced without the need to use forces near the upper limit of the press, thus avoiding premature wear on the tabletting machine. Thus, once a suitable size for a given tablet formulation has been chosen, the granule size selected has been shown to determine the crushing strength of the tablet.

Climatic variables and altitude as predictors of anuran species richness and number of reproductive modes in Brazil

Because anuran species are highly dependent on environmental variables, we hypothesized that anuran species richness and the number of reproductive modes from different Brazilian localities vary according to climatic and altitudinal variables. Published data were compiled from 36 Brazilian localities and climatic and altitudinal data were extracted from an available database. A partial redundancy analysis (pRDA) showed that 23.5% of the data set's variation was explained by climatic and altitudinal data, while the remaining 76.5% remained unexplained. This analysis suggests that other factors not analysed herein may also be important for predicting anuran species richness and the number of reproductive modes in Brazil. Altitude and total annual rainfall were positively correlated with anuran species richness and the number of reproductive modes, and total annual rainfall was strongly associated with these two biotic variables in the triplot of pRDA. The positive association of total annual rainfall and the negative association of the concentration of annual rainfall were already expected based on physiological and reproductive requirements of anurans. On the other hand, temperature was not associated with richness or the number of reproductive modes. Copyright © 2010 Cambridge University Press.

Legislación comparada sobre personas mayores en Centroamérica y la República Dominicana

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Gasto, inversión y financiamiento para el desarrollo sostenible en Argentina

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Spatial clustering applied to health area

The significant volume of work accidents in the cities causes an expressive loss to society. The development of Spatial Data Mining technologies presents a new perspective for the extraction of knowledge from the correlation between conventional and spatial attributes. One of the most important techniques of the Spatial Data Mining is the Spatial Clustering, which clusters similar spatial objects to find a distribution of patterns, taking into account the geographical position of the objects. Applying this technique to the health area, will provide information that can contribute towards the planning of more adequate strategies for the prevention of work accidents. The original contribution of this work is to present an application of tools developed for Spatial Clustering which supply a set of graphic resources that have helped to discover knowledge and support for management in the work accidents area. © 2011 IEEE.

CdMoO 4 synthesized by a microwave-assisted hydrothermal method and their photoluminescence and photocatality properties

In this work CdMoO 4 nanoparticles were obtained under hydrothermal conditions using microwave radiation (2.45 GHz) (MH) at 100°C for different times. These powders were analyzed by X-ray diffraction (XRD), Field-emisson gum scanning electron microscopy (FEG-SEM), Ultraviolet-visible (UV-vis) absorption spectroscopy and photoluminescence (PL) measurements. XRD pattern confirmed that the pure CdMoO 4 phases were obtained. FEG-SEM powders present large-scale and homogeneous particles with microspheres-like morphology. UV-vis results were employed to determine the optical band gap these materials. Also, it showed existence of photoluminescence (PL) emission in the green wavelength range of 540-546 nm. Photocatalytic activity of CdMoO 4 nanocrystals was examined by monitoring the degradation of rhodamine B dye.

Lysine and methionine + cystine for laying hens during the post-molting phase

One experiment was conducted to evaluate the effect of using different lysine and methionine + cystine levels on body weight recovery, performance, and egg quality of laying hens during the postmolting period. In this trial, 432 Isa Brown layers, with 72 weeks of age, were distributed in 54 cages according to a completely randomized design with six treatments and nine replicates of eight birds each. During the resting period, six diets with different digestible lysine and methionine + cystine levels were used, as follows: 0.48% digestible lysine and 0.43% methionine + cystine; 0.48% digestible lysine and 0.47% methionine + cystine; 0.48% digestible lysine and 0.52% methionine + cystine; 0.56% digestible lysine and 0.50% methionine + cystine; 0.56% digestible lysine and 0.56% methionine + cystine; 0.56% digestible lysine and 0.62% methionine + cystine. Data were submitted to analysis of variance and means were compared at by Tukey's test at 5% probability level. The different lysine and methionine + cystine levels in the diets fed during the resting period significantly influenced layer performance. The diet containing 0.56% lysine and 0.56% methionine + cystine promoted higher egg weight eggs during the second production cycle.

Actividades de asistencia técnica en América Latina durante 1956

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Rootstocks resistant to Meloidogyne incognita and compatibility of grafting in net melon

Due to the few studies about grafting in net melon, in order to obtain better control of soil pathogens, the aim of the present study was to evaluate 16 genotypes of Cucurbitaceae: Benincasa hispida, Luffa cylindrica, pumpkin 'Jacarezinho', pumpkin 'Menina Brasileira', squash 'Exposição', squash 'Coroa', pumpkin 'Canhão Seca', pumpkin 'Squash', pumpkin 'Enrrugado Verde', pumpkin 'Mini Paulista', pumpkin 'Goianinha', watermelon 'Charleston Gray', melon 'Rendondo Gaucho', melon 'Redondo Amarelo', cucumber 'Caipira HS' and cucumber 'Caipira Rubi', regarding to compatibility of grafting in net melon and resistance to Meloidogyne incognita, based on the reproduction factor (RF), according to Oostenbrink (1966). To assess resistance, the seedlings were transplanted to ceramic pots and inoculated with 300/mL eggs and/or second stage juveniles of M. incognita. At 50 days after transplanting, the plants were removed from the pots and the resistance was evaluated. The compatibility between resistant rootstock and grafts of net melon was determined by performing simple cleft grafting, in a commercial net melon hybrid of great market acceptance and susceptible to M. incognita (Bonus no. 2). The genotypes Luffa cylindrica, pumpkin 'Goianinha', pumpkin 'Mini-Paulista', melon 'Redondo Amarelo', watermelon 'Charleston Gray' are resistant to the nematode M. incognita. The better compatibilities occurred with the rootstocks melon 'Amarelo', which presented 100% of success, followed by pumpkin 'Mini-Paulista' with 94%. On the other hand, Sponge gourd, watermelon 'Charleston Gray' and pumpkin 'Goianinha' showed low graft take percentages of 66%, 62% and 50%, respectively.