NAD Biosynthesis Evolution in Bacteria: Lateral Gene Transfer of Kynurenine Pathway in Xanthomonadales and Flavobacteriales

The biosynthesis of quinolinate, the de novo precursor of nicotinamide adenine dinucleotide (NAD), may be performed by two distinct pathways, namely, the bacterial aspartate (aspartate-to-quinolinate) and the eukaryotic kynurenine (tryptophan-to-quinolinate). Even though the separation into eukaryotic and bacterial routes is long established, recent genomic surveys have challenged this view, because certain bacterial species also carry the genes for the kynurenine pathway. In this work, both quinolinate biosynthetic pathways were investigated in the Bacteria clade and with special attention to Xanthomonadales and Bacteroidetes, from an evolutionary viewpoint. Genomic screening has revealed that a small number of bacterial species possess some of the genes for the kynurenine pathway, which is complete in the genus Xanthomonas and in the order Flavobacteriales, where the aspartate pathway is absent. The opposite pattern (presence of the aspartate pathway and absence of the kynurenine pathway) in close relatives (Xylella ssp. and the order Bacteroidales, respectively) points to the idea of a recent acquisition of the kynurenine pathway through lateral gene transfer in these bacterial groups. In fact, sequence similarity comparison and phylogenetic reconstruction both suggest that at least part of the genes of the kynurenine pathway in Xanthomonas and Flavobacteriales is shared by eukaryotes. These results reinforce the idea of the role that lateral gene transfer plays in the configuration of bacterial genomes, thereby providing alternative metabolic pathways, even with the replacement of primary and essential cell functions, as exemplified by NAD biosynthesis.

An artificial nanoemulsion carrying paclitaxel decreases the transplant heart vascular disease: A study in a rabbit graft model

Objective: In previous studies cholesterol-rich nanoemulsions (LDE) resembling low-density lipoprotein were shown to concentrate in atherosclerotic lesions of rabbits. Lesions were pronouncedly reduced by treatment with paclitaxel associated with LDE. This study aimed to test the hypothesis of whether LDE-paclitaxel is able to concentrate in grafted hearts of rabbits and to ameliorate coronary allograft vasculopathy after the transplantation procedure. Methods: Twenty-one New Zealand rabbits fed 0.5% cholesterol were submitted to heterotopic heart transplantation at the cervical position. All rabbits undergoing transplantation were treated with cyclosporin A (10 mg . kg(-1) . d(-1) by mouth). Eleven rabbits were treated with LDE-paclitaxel (4 mg/kg body weight paclitaxel per week administered intravenously for 6 weeks), and 10 control rabbits were treated with 3 mL/wk intravenous saline. Four control animals were injected with LDE labeled with [(14)C]-cholesteryl oleate ether to determine tissue uptake. Results: Radioactive LDE uptake by grafts was 4-fold that of native hearts. In both groups the coronary arteries of native hearts showed no stenosis, but treatment with LDE-paclitaxel reduced the degree of stenosis in grafted hearts by 50%. The arterial luminal area in grafts of the treated group was 3-fold larger than in control animals. LDE-paclitaxel treatment resulted in a 7-fold reduction of macrophage infiltration. In grafted hearts LDE-paclitaxel treatment reduced the width of the intimal layer and inhibited the destruction of the medial layer. No toxicity was observed in rabbits receiving LDE-paclitaxel treatment. Conclusions: LDE-paclitaxel improved posttransplantation injury to the grafted heart. The novel therapeutic approach for heart transplantation management validated here is thus a promising strategy to be explored in future clinical studies. (J Thorac Cardiovasc Surg 2011;141:1522-8)

DNA damage induced by the anthracycline cosmomycin D in DNA repair-deficient cells

Anthracyclines have been widely used as antitumor agents, playing a crucial role in the successful treatment of many types of cancer, despite some side effects related to cardiotoxicity. New anthracyclines have been designed and tested, but the first ones discovered, doxorubicin and daunorubicin, continue to be the drugs of choice. Despite their extensive use in chemotherapy, little is known about the DNA repair mechanisms involved in the removal of lesions caused by anthracyclines. The anthracycline cosmomycin D is the main product isolated from Streptomyces olindensis, characterized by a peculiar pattern of glycosylation with two trisaccharide rings attached to the A ring of the tetrahydrotetracene. We assessed the induction of apoptosis (Sub-G(1)) by cosmomycin D in nucleotide excision repair-deficient fibroblasts (XP-A and XP-C) as well as the levels of DNA damage (alkaline comet assay). Treatment of XP-A and XP-C cells with cosmomycin D resulted in apoptosis in a time-dependent manner, with highest apoptosis levels observed 96 h after treatment. The effects of cosmomycin D were equivalent to those obtained with doxorubicin. The broad caspase inhibitor Z-VAD-FMK strongly inhibited apoptosis in these cells, and DNA damage induced by cosmomycin D was confirmed by alkaline comet assay. Cosmomycin D induced time-dependent apoptosis in nucleotide excision repair-deficient fibroblasts. Despite similar apoptosis levels, cosmomycin D caused considerably lower levels of DNA damage compared to doxorubicin. This may be related to differences in structure between cosmomycin D and doxorubicin.

Ab initio calculation of the BCC Fe-Al-Mo (Iron-Aluminum-Molybdenum) phase diagram: Implications for the nature of the tau(2) phase

The metastable phase diagram of the BCC-based ordering equilibria in the Fe-Al-Mo system has been calculated via a truncated cluster expansion, through the combination of Full-Potential-Linear augmented Plane Wave (FP-LAPW) electronic structure calculations and of Cluster Variation Method (CVM) thermodynamic calculations in the irregular tetrahedron approximation. Four isothermal sections at 1750 K, 2000 K, 2250 K and 2500 K are calculated and correlated with recently published experimental data on the system. The results confirm that the critical temperature for the order-disorder equilibrium between Fe(3)Al-D0(3) and FeAl-B2 is increased by Mo additions, while the critical temperature for the FeAl-B2/A2 equilibrium is kept approximately invariant with increasing Mo contents. The stabilization of the Al-rich A2 phase in equilibrium with overstoichiometric B2-(Fe,Mo)Al is also consistent with the attribution of the A2 structure to the tau(2) phase, stable at high temperatures in overstoichiometric B2-FeAl. (C) 2009 Elsevier Ltd. All rights reserved.

Neoproterozoic geodynamic evolution of SW-Gondwana: a southern African perspective

Our current understanding of the tectonic history of the principal Pan-African orogenic belts in southwestern Africa, reaching from the West Congo Belt in the north to the Lufilian/Zambezi, Kaoko, Damara, Gariep and finally the Saldania Belt in the south, is briefly summarized. On that basis, possible links with tectono-stratigraphic units and major structures on the eastern side of the Rio de la Plata Craton are suggested, and a revised geodynamic model for the amalgamation of SW-Gondwana is proposed. The Rio de la Plata and Kalahari Cratons are considered to have become juxtaposed already by the end of the Mesoproterozoic. Early Neoproterozoic rifting led to the fragmentation of the northwestern (in today`s coordinates) Kalahari Craton and the splitting off of several small cratonic blocks. The largest of these ex-Kalahari cratonic fragments is probably the Angola Block. Smaller fragments include the Luis Alves and Curitiba microplates in eastern Brazil, several basement inliers within the Damara Belt, and an elongate fragment off the western margin, named Arachania. The main suture between the Kalahari and the Congo-So Francisco Cratons is suspected to be hidden beneath younger cover between the West Congo Belt and the Lufilian/Zambezi Belts and probably continues westwards via the Cabo Frio Terrane into the Goias magmatic arc along the Brasilia Belt. Many of the rift grabens that separated the various former Kalahari cratonic fragments did not evolve into oceanic basins, such as the Northern Nosib Rift in the Damara Belt and the Gariep rift basin. Following latest Cryogenian/early Ediacaran closure of the Brazilides Ocean between the Rio de la Plata Craton and the westernmost fragment of the Kalahari Craton, the latter, Arachania, became the locus of a more than 1,000-km-long continental magmatic arc, the Cuchilla Dionisio-Pelotas Arc. A correspondingly long back-arc basin (Marmora Basin) on the eastern flank of that arc is recognized, remnants of which are found in the Marmora Terrane-the largest accumulation of oceanic crustal material known from any of the Pan-African orogenic belts in the region. Corresponding foredeep deposits that emerged from the late Ediacaran closure of this back-arc basin are well preserved in the southern areas, i.e. the Punta del Este Terrane, the Marmora Terrane and the Tygerberg Terrane. Further to the north, present erosion levels correspond with much deeper crustal sections and comparable deposits are not preserved anymore. Closure of the Brazilides Ocean, and in consequence of the Marmora back-arc basin, resulted from a change in the Rio de la Plata plate motion when the Iapetus Ocean opened between the latter and Laurentia towards the end of the Ediacaran. Later break-up of Gondwana and opening of the modern South Atlantic would have followed largely along the axis of the Marmora back-arc basin and not along major continental sutures.

Relics of eclogite facies assemblages in the Ceara Central Domain, NW Borborema Province, NE Brazil: Implications for the assembly of West Gondwana

The Borborema Province, in the NE of Brazil, is a rather complex piece in the Brazil-Africa puzzle as it represents the junction of the Dahomeyide/Pharusian, Central African, Aracuai and Brasilia fold belts located between the West-African/Sao Luis, Congo/Sao Francisco and Amazonas craton. The correlation between the Dahomeyides from W-Africa (Ghana, Benin, Togo, and Mali) and the Borborema Province involves the Medio Coreau and Central Ceara domains. The inferred continuation of the main oceanic suture zone exposed in the Dahomeyides of W Africa is buried beneath the Phanerozoic Parnaiba Basin in Brazil (northwest of the Medio Coreau domain) where some high density gravity anomalies may represent hidden remnants of an oceanic suture. In addition to this major suture a narrow, nearly continuous strip composed of mainly mafic pods containing relics of eclogite-facies assemblages associated with partially migmatized granulite-facies metapelitic gneisses has been found further east in the NW Borborema Province. These high pressure mafic rocks, interpreted as retrograded eclogites, are located between the Transbrasiliano Lineament and the Santa Quiteria continental arc and comprise primitive to evolved arc-related rocks with either arc- or MORB-type imprints that can indicate either deep subduction of oceanic lithosphere or roots of continental and oceanic magmatic arcs. Average peak P-T conditions under eclogite-facies metamorphism (T=770 degrees C and P = 17.3 kbar) were estimated using garnet-clinopyroxene thermometry and Jd content in clinopyroxene. Transition to granulite-facies conditions, as well as later widespread re-equilibration under amphibolite facies, were registered both in the basic and the metapelitic rocks and suggest a clockwise P-T path characterized by an increase in temperature followed by strong decompression. A phenomenon possibly related to the exhumation of a highly thickened crust associated with the suturing of the Medio Coreau and Central Ceara domains, two distinct crustal blocks separated by the Transbrasiliano Lineament. (C) 2009 International Association for Gondwana Research. Published by Elsevier B.V. All rights reserved.

Dating coeval mafic magmatism and ultrahigh temperature metamorphism in the Anapolis-Itaucu Complex, Central Brazil

Dating granulites has always been of great interest because they represent one of the most extreme settings of an orogen. Owing to the resilience of zircon, even in such severe environments, the link between P-T conditions and geological time is possible. However, a challenge to geochronologists is to define whether the growth of new zircon is related to pre- or post-P-T peak conditions and which processes might affect the (re) crystallization. In this context, the Anapolis-Itaucu Complex, a high-grade complex in central Brazil with ultrahigh temperature (UHT) granulites, may provide valuable information within this topic. The Anapolis-Itaucu Complex (AIC) includes ortho- and paragranulites, locally presenting UHT mineral assemblages, with igneous zircon ages varying between 760 and 650 Ma and metamorphic overgrowths dated at around 650-640 Ma. Also common in the Anapolis-Itaucu Complex are layered mafic-ultramafic complexes metamorphosed under high-grade conditions. This article presents the first geological and geochronological constraints of three of these layered complexes within the AIC, the Damolandia, Taquaral and Goianira-Trindade complexes. U-Pb (LA-MC-ICPMS, SHRIMP and ID-TIMS) zircon analyses reveal a spread of concordant ages spanning within an age interval of similar to 80 Ma with an ""upper"" intercept age of similar to 670 Ma. Under cathodoluminescence imaging, these crystals show partially preserved primary sector zoning, as well as internal textures typical of alteration during high-grade metamorphism, such as inward-moving boundaries. Zircon grains reveal homogeneous initial (176)Hf/(177)Hf values in distinct crystal-scale domains in all samples. Moreover. Hf isotopic ratios show correlation neither with U-Pb ages nor with Th/U ratios, suggesting that zircon grains crystallized during a single growth event. It is suggested, therefore, that the observed spread of concordant U-Pb ages may be related to a memory effect due to coupled dissolution-reprecipitation process during high grade metamorphism. Therefore, understanding the emplacement and metamorphism of this voluminous mafic magmatism is crucial as it may represent an additional heat source for the development of the ultrahigh temperature paragenesis recorded in the paragranulites. (C) 2010 Elsevier B.V. All rights reserved.

Surfactant degradation by a catechol-driven Fenton reaction

The addition of 0.5 mM catechol is shown to accelerate the degradation and mineralization of the anionic surfactant DOWFaX (TM) 2A1 (sodium dodecyldiphenyloxide disulfonate) under conventional Fenton reaction conditions (Fe(II) plus H(2)O(2) at pH 3). The catalytic effect causes a 3-fold increase in the initial rate (up to ca. 20 min) of conversion of the surfactant to oxidation products (apparent first-order rate constants of 0.021 and 0.061 min(-1) in the absence and presence of catechol, respectively). Although this catalytic rate increase persists for a certain amount of time after complete disappearance of catechol itself (ca. 8 min), the reaction rate begins to decline slowly after the initial 20 min towards that observed in the absence of added catechol. Total organic carbon (TOC) measurements of net mineralization and cyclic voltammetric and high performance liquid chromatographic (HPLC) measurements of the initial rate of reaction of catechol and the surfactant provide insight into the role of catechol in promoting the degradation of the surfactant and of degradation products as the eventual inhibitors of the Fenton reaction. (C) 2010 Elsevier B.V. All rights reserved.

A trajetória do ambiente jurídico-institucional do setor de software no Brasil e na Índia: identidades, diferenças e repercussões

Há mais de 30 anos o Brasil tem desenvolvido políticas específicas para o setor de informática, desde a Política Nacional de Informática da década de 70, passando pelo Período de Reserva de Mercado dos anos 80 e, nos dias de hoje, em que as Tecnologias de Informação e Comunicação (TIC) são tidas como uma das áreas prioritárias na Política Industrial. Dentre as metas atuais, destaca-se o foco na ampliação do volume de exportações de software e serviços. Contudo, apesar dessas pretensões, o país não tem tido destaque internacional expressivo para o setor. Por outro lado, a Índia, também considerada como um país emergente, figurando na lista dos BRIC, foi responsável pela exportação de cerca de US$47 bilhões em software e serviços de Tecnologia da Informação (TI) em 2009, se destacando como um país protagonista no mercado internacional do setor. A implementação de uma indústria tecnicamente sofisticada como a do software, que exige um ambiente propício à inovação, em um país em desenvolvimento como a Índia chama a atenção. De certo existiram arranjos jurídico-institucionais que foram utilizados naquele país. Quais? Em que medida tais arranjos ajudaram no desenvolvimento indiano do setor? E no Brasil? Este trabalho parte da hipótese de que o ambiente jurídico-institucional desses países definiu fluxos de conhecimento distintos, influenciando o tipo de desenvolvimento do setor de software de cada um. Averiguar como, entre outros fatores sócio-econômicos, esses arranjos jurídico-institucionais influenciaram na conformação diversa de fluxos de conhecimento é o objetivo específico desta pesquisa. Entende-se aqui como ambiente jurídico-institucional todas as regulamentações que estabelecem instituições, diretrizes e condições comuns para determinado tema. Partindo do pressuposto de que o setor de software desenvolve atividades intensivas em conhecimento, para cada país em questão, serão analisados apenas arranjos jurídico-institucionais que tiveram, ou têm, poder de delimitar o fluxo de conhecimento referente ao setor, sejam eles provenientes de políticas comerciais (de exportação e importação, ou de propriedade intelectual) ou de políticas de investimento para inovação. A questão fundamental ultrapassa o debate se o Estado deve ou não intervir, para focar-se na análise sobre os diferentes tipos de envolvimento observados e quais os seus efeitos. Para tal, além de revisão bibliográfica, foi feita uma pesquisa de campo na Índia (Delhi, Mumbai, Bangalore) e no Brasil (São Paulo, Brasília e Rio de Janeiro), onde foram conduzidas entrevistas com empresas e associações de software, gestores públicos e acadêmicos que estudam o setor.

Uma análise do discurso dos bancos à luz do conceito de lealdade em marketing de relacionamento

O objetivo deste trabalho é verificar se há ou não congruência entre a ideia de lealdade, manifestada no discurso dos bancos, e o seu conceito em marketing de relacionamento, identificando qual o sentido desse constructo nas mensagens das instituições bancárias, além de identificar, também, quais os fatores que levam os clientes a manterem relacionamento duradouro com bancos. O estudo é de caráter exploratório, e foi conduzido com entrevistas individuais com clientes pessoa física e gerentes de bancos públicos e privados de Brasília/DF, com o intuito de explorar o relacionamento entre cliente-banco, para uma melhor orientação na direção das análises dos dados obtidos. Os dados foram coletados junto a 11 entrevistados de ambos os sexos, residentes em Brasília/DF, durante os meses de março e abril de 2011. Para atingir os objetivos propostos, o método de pesquisa adotado foi qualitativo, com foco no valor informacional da mensagem propriamente dita, das palavras, argumentos e idéias nela expressos, utilizando-se uma forma interpretativa para a análise dos dados. Os resultados demonstraram o afastamento conceitual entre a ideia de lealdade dos bancos, definida como algo ligado ao entendimento de que o cliente, confiante em seu banco, está satisfeito e não o deixa, e o seu conceito em marketing de relacionamento, que a define como um profundo compromisso do cliente em recomprar um produto/serviço consistentemente no futuro, pois constatou-se que os clientes bancários, independentemente de fatores como o tempo gasto ou o esforço associados à troca de fornecedores, são sensíveis a aumento substancial da tarifação, não tem compromisso de recompra com banco e nem de compra junto a um único banco. Em decorrência, se pode concluir que não são fiéis/leais. Como fatores responsáveis por relacionamento duradouro com bancos, verificou-se, como principais, a qualidade do atendimento prestado pelo banco e a reciprocidade existente no relacionamento, ambos são constituintes dos sentimentos de satisfação e confiança nos clientes bancários. Conclui-se o trabalho, fazendo-se recomendações com a intenção de beneficiar e desenvolver os gestores deste segmento.

Autonomia e futuro da perícia oficial na percepção dos peritos criminais

Este estudo teve como objetivo levantar a visão de futuro da Criminalistiea brasileira conforme as pereepçõcs autonómieas de peritos oficiais empregando como parad igma os efeitos da Lei 12.03012009, que atribuiu a autonomia técnica, científica ti funcional " estes profissionais. Para isso, rea lizou-se uma pesquisa bibliográfica c de campo. O univcrso da pesquisa foi os Peritos Oficiai , . /\ amo,tra foi fommda por 39 peritos Criminais, um grupo principal COl1lpo~to por 31 peritos de carreira incluindo gestores locais de Instituições Federais (Polícia FctJeral, Polícia do Distrito Federal) e c.s tadoaiS (Polícia Civil e Secretaria de Scgurança Pública) c um grupo complcnlenlar composto por Gestores (04) c Lideranças (04) Nacionai~ destes órgãos. Ao grupo principal foi aplicado um testc de evocação com as palavras "Autonomia" e "Pcricia". como fase preparatória às entrevistas semi cSlrutura-se que a Perícia Ofieial se identifica em essência que'; 3 descoberta da verdade a cerca de um fa to comunicado como crime c seu papel social que cons ta da comunicação olicial desta verdade ao Magistrado por meio do laudo c se diferencia pela percepção de autonomia c conseqüente v;slo de fulUro. Os peritos sob regulamentaçlo Federal pereehem a au tonomia segundo a lei alcançada c acreditam na possihilidadc de autonomia administra tiva inseridos no órgão policial, enquanto os p<:ritos vincolados aos estados buscam maior aotonomia como organi7,1Ção i"d cpcndcnt~, integrada e de âmbito nacional, e mais próxima ao Jodieiário, seguindo orientação da ONU (Protocolo de Istamb"l) c do Decreto Lei 7.037/09. Para todos entrevistados a Lei 12.030/09 apenas legitimou oma situaçlo de fato.

Por que mudar de cidade?: uma análise da satisfação e desejo de mudança de lotação entre peritos da Polícia Federal em Belém e Brasília

Esta pesquisa teve por objetivo verificar até que ponto o grau de satisfação no trabalho dos Peritos Criminais Federais (PCFs) lotados na Região Norte do Brasil está relacionado com o alto índice de remoção destes servidores para outras localidades. Inicialmente foi realizada uma revisão bibliográfica sobre o tema satisfação no trabalho, incluindo aspectos sobre motivação e as variáveis relacionadas ao tema. Em seguida, em uma primeira etapa, foi aplicada a escala de satisfação no trabalho (EST) proposta por Siqueira (2008), onde são avaliadas cinco dimensões do trabalho: relacionamento com os colegas, relacionamento com a chefia, a natureza do trabalho, o salário e o sistema de promoções dentro da organização. Foram também aplicados questionários para caracterização da amostra e para verificação da intenção de remoção dos peritos para outra unidade. Comparou-se ainda a produção de laudos dos peritos lotados na Região Norte com a produção média do Brasil. A partir dos resultados encontrados, onde se tem a maioria dos Peritos satisfeitos, e com produção média próxima à média do Brasil, porém querendo ser removidos, fez-se necessária a realização de entrevistas em profundidade com amostra reduzida a fim de se entender este fenômeno, a princípio contraditório. As entrevistas foram realizadas, em uma segunda etapa da pesquisa, com peritos lotados em Belém-PA, cidade da região estudada e em Brasília-DF, cidade desejada como futura lotação pela maioria dos peritos que responderam ao questionário da primeira etapa. Os resultados das entrevistas apontam evidências de que a família dos Peritos, aqui entendida como pais, avós, irmãos, primos dentre outros, é a principal razão pela forte intenção de mudança de Unidade de Criminalística, apesar de estarem satisfeitos em seu trabalho. Assim como, é o principal fator apontado para a permanência dos peritos em Brasília, já que nenhum dos entrevistados tem a intenção de ser removido para outro local. Outras razões contribuem, em menor escala para esse desejo, tais como as condições de trabalho, o desenvolvimento da cidade, a segurança na cidade e a qualidade de vida na cidade. Por fim, sugestões colhidas nas entrevistas para melhoria da Criminalística na Polícia Federal são apresentadas, assim como propostas para novas pesquisas.

O Governo JK e a revista Manchete: a criação do mito dos anos dourados

Os anos entre 1956 a 1961 foram pródigos para o desenvolvimento do Brasil. O governo JK consolidou a industrialização brasileira, criou Brasília e foi considerado um caso singular na política contemporânea, por ser o único governo civil que começou e terminou seu mandato segundo as regras constitucionais, até o período da ditadura militar. Através da rememoração, lembramos de fatos históricos, que através da narrativa da revista Manchete, contribuíram para a criação de uma imagem positiva de Juscelino Kubitschek e de seu governo. As edições constantes de sua imagem de grande estadista justificam a existência, na memória coletiva, do mito político. Sua trajetória foi caracterizada por sua simpatia, seu poder de persuasão e pela força de sua oratória durante o seu governo e posteriormente calada pela ditadura militar, implantada no Brasil em 1964. Manchete deu forma, através de suas fotorreportagens, aos acontecimentos de um país em acelerado processo de desenvolvimento, contribuindo desta maneira, com a criação do mito dos anos dourados.

Differences in the perception of interest representation: a comparision of Brasília and Brussels lobbying activity

The research topic of this paper is focused on the analysis of how trade associations perceive lobbying in Brussels and in Brasília. The analysis will be centered on business associations located in Brasília and Brussels as the two core centers of decision-making and as an attraction for the lobbying practice. The underlying principles behind the comparison between Brussels and Brasilia are two. Firstof all because the European Union and Brazil have maintained diplomatic relations since 1960. Through these relations they have built up close historical, cultural, economic and political ties. Their bilateral political relations culminated in 2007 with the establishment of a Strategic Partnership (EEAS website,n.d.). Over the years, Brazil has become a key interlocutor for the EU and it is the most important market for the EU in Latin America (European Commission, 2007). Taking into account the relations between EU and Brazil, this research could contribute to the reciprocal knowledge about the perception of lobby in the respective systems and the importance of the non-market strategy when conducting business. Second both EU and Brazilian systems have a multi-level governance structure: 28 Member States in the EU and 26 Member States in Brazil; in both systems there are three main institutions targeted by lobbying practice. The objective is to compare how differences in the institutional environments affect the perception and practice of lobbying, where institutions are defined as ‘‘regulative, normative, and cognitive structures and activities that provide stability and meaning to social behavior’’ (Peng et al., 2009). Brussels, the self-proclaimed "Capital of Europe”, is the headquarters of the European Union and has one of the highest concentrations of political power in the world. Four of the seven Institutions of the European Union are based in Brussels: the European Parliament, the European Council, the Council and the European Commission (EU website, n.d.). As the power of the EU institutions has grown, Brussels has become a magnet for lobbyists, with the latest estimates ranging from between 15,000 and 30,000 professionals representing companies, industry sectors, farmers, civil society groups, unions etc. (Burson Marsteller, 2013). Brasília is the capital of Brazil and the seat of government of the Federal District and the three branches of the federal government of Brazilian legislative, executive and judiciary. The 4 city also hosts 124 foreign embassies. The presence of the formal representations of companies and trade associations in Brasília is very limited, but the governmental interests remain there and the professionals dealing with government affairs commute there. In the European Union, Brussels has established a Transparency Register that allows the interactions between the European institutions and citizen’s associations, NGOs, businesses, trade and professional organizations, trade unions and think tanks. The register provides citizens with a direct and single access to information about who is engaged in This process is important for the quality of democracy, and for its capacity to deliver adequate policies, matching activities aimed at influencing the EU decision-making process, which interests are being pursued and what level of resources are invested in these activities (Celgene, n.d). It offers a single code of conduct, binding all organizations and self-employed individuals who accept to “play by the rules” in full respect of ethical principles (EC website, n.d). A complaints and sanctions mechanism ensures the enforcement of the rules and addresses suspected breaches of the code. In Brazil, there is no specific legislation regulating lobbying. The National Congress is currently discussing dozens of bills that address regulation of lobbying and the action of interest groups (De Aragão, 2012), but none of them has been enacted for the moment. This work will focus on class lobbying (Oliveira, 2004), which refers to the performance of the federation of national labour or industrial unions, like CNI (National Industry Confederation) in Brazil and the European Banking Federation (EBF) in Brussels. Their performance aims to influence the Executive and Legislative branches in order to defend the interests of their affiliates. When representing unions and federations, class entities cover a wide range of different and, more often than not, conflicting interests. That is why they are limited to defending the consensual and majority interest of their affiliates (Oliveira, 2004). The basic assumption of this work is that institutions matter (Peng et al, 2009) and that the trade associations and their affiliates, when doing business, have to take into account the institutional and regulatory framework where they do business.