Qing chao quan shi /

Mode of access: Internet.

Fen men ji zhu Du Gongbu shi : 25 juan /

Fu: Nian pu : 1 juan / Lü Dafang, Cai Xingzong, Lu Yin zhuan.

Ying jiu chao juan zi yuan ben Yu pian ling juan /

On double leaves, oriental style, in case.

Juden

Detached from Allgemeine Encyklopädie der Wissenschaften und Künste, hrsg. von Ersch und Gruber. Zweite Section, H-N.

Li dai hua shi hui zhuan /

Mode of access: Internet.

Xu Zi zhi tong jian [220 juan]

Mode of access: Internet.

Shi yong guo yu wen fa.

Nei rong you ci lun, ju lun ji dan ju, fu ju deng.

Jing xuan Lu Fangweng shi ji : qian ji 10 juan, hou ji 8 juan, bie ji 1 juan /

Ju Liu shi Jia ye tang cang Hongzhi 10 nian kan ben ying yin.

Da Dai li ji : 13 juan /

Ju Wuxi Sun shi Xiao lü tian cang Ming Wujun Yuan shi Jia qu tang kan ben ying yin.

Högläsning – En gemensam väg in i språket : En multipel fallstudie av fyra lärares medvetna högläsning i årskurs 4 och i årskurs 6

Studien undersöker och jämför hur fyra lärare arbetar medvetet med sin högläsning i klassrummet och ifall deras arbete förändras mellan årskurs 4 och årskurs 6. Undersökningen är disponerad som en multipel fallstudie, där varje enskilt fall först analyseras separat för att därpå korsanalyseras. För att kunna besvara studiens syfte och frågeställningar genomfördes en empirisk undersökning där de fyra fallen studerades genom metoderna enkät, observation, intervju och deltagarvalidering. Därefter analyserades fallen utifrån den sociokulturella teorin om lärande, skolans styrdokument och tidigare forskning inom ämnesområdet. Resultaten ger en positiv inblick i lärares arbete med högläsning i klassrummet. De didaktiska val som ligger bakom de deltagande lärarnas högläsning går att koppla till läroplanens syfte och centrala innehåll. Lärarna verkar arbeta varierat och eleverna tränas med högläsningen som utgångspunkt i läroplanens övergripande förmågor. Lärarna har alla märkt ett generellt minskat läsintresse hos barn och elever men anser sig överlag inte märka så mycket av detta i sina egna klassrum, vilket de kopplar till att de aktivt arbetar med att läsa högt för eleverna. Vidare anser de att elevernas språkutveckling och ordförståelse gynnas av högläsning då de får lyssna till det skrivna ordet. Studiens lärare är överens om att det är viktigt att avsätta tid till högläsning i undervisningen och använder sig av en dialogisk uppläsning i sina klassrum, där högläsningen blir ett pedagogiskt verktyg. Endast en av lärarna säger sig uttryckligen arbeta med specifika metoder och strategier utifrån ämnesdidaktisk forskning, men vid besöken observerades att även de övriga lärarna intuitivt arbetar implicit med lässtrategier i sin undervisning. Gemensamt för lärarna är att de ofta väljer att arbeta ämnesintegrerat och att högläsningen blir en naturlig del i ett tematiskt arbete. De menar också att behovet av deras högläsning i undervisningen snarast ökar i och med att eleverna blir äldre, då de möts av mer komplexa texter ju äldre de blir.

Tetracycline-Inducible packaging cell line for production of Flavivirus replicon particles.

We have previously developed replicon vectors derived from the Australian flavivirus Kunjin that have a unique noncytopathic nature and have been shown to direct prolonged high-level expression of encoded heterologous genes in vitro and in vivo and to induce strong and long-lasting immune responses to encoded immunogens in mice. To facilitate further applications of these vectors in the form of virus-like particles (VLPs), we have now generated a stable BHK packaging cell line, tetKUNCprME, carrying a Kunjin structural gene cassette under the control of a tetracycline-inducible promoter. Withdrawal of tetracycline from the medium resulted in production of Kunjin structural proteins that were capable of packaging transfected and self-amplified Kunjin replicon RNA into the secreted VLPs at titers of up to 1.6 x 10(9) VLPs per ml. Furthermore, secreted KUN replicon VLPs from tetKUNCprME cells could be harvested continuously for as long as 10 days after RNA transfection, producing a total yield of more than 1010 VLPs per 106 transfected cells. Passaging of VLPs on Vero cells or intracerebral injection into 2- to 4-day-old suckling mice illustrated the complete absence of any infectious Kunjin virus. tetKUNCprME cells were also capable of packaging replicon RNA from closely and distantly related flaviviruses, West Nile virus and dengue virus type 2, respectively. The utility of high-titer KUN replicon VLPs was demonstrated by showing increasing CD8(+)-T-cell responses to encoded foreign protein with increasing doses of KUN VLPs. A single dose of 2.5 x 10(7) VLPs carrying the human respiratory syncytial virus M2 gene induced 1,400 CD8 T cells per 10(6) splenocytes in an ex vivo gamma interferon enzyme-linked immunospot assay. The packaging cell line thus represents a significant advance in the development of the noncytopathic Kunjin virus replicon-based gene expression system and may be widely applicable to the basic studies of flavivirus RNA packaging and virus assembly as well as to the development of gene expression systems based on replicons from different flaviviruses.

Defenses against oxidative stress in Neisseria gonorrhoeae and Neisseria meningitidis: Distinctive systems for different lifestyles

Defenses against oxidative stress are crucial for the survival of the pathogens Neisseria meningitidis and Neisseria gonorrhoeae. An Mn(II) uptake system is involved in manganese (Mn)-dependent resistance to superoxide radicals in N. gonorrhoeae. Here, we show that accumulation of Mn also confers resistance to hydrogen peroxide killing via a catalase-independent mechanism. An mntC mutant of N. meningitidis is susceptible to oxidative killing, but supplementation of growth media with Mn does not enhance the organism's resistance to oxidative killing. N. meningitidis is able to grow in the presence of millimolar levels of Mn ion, in contrast to N. gonorrhoeae, whose growth is retarded at Mn concentrations >100 mumol/L, indicating that Mn homeostasis in the 2 species is probably quite different. N. meningitidis superoxide dismutase B plays a role in protection against oxidative killing. However, a sodC mutant of N. meningitidis is no more sensitive to oxidative killing than is the wild type. A cytochrome c peroxidase (Ccp) is present in N. gonorrhoeae but not in N. meningitidis. Investigations of a ccp mutant revealed a role for Ccp in protection against hydrogen peroxide killing. These differences in oxidative defenses in the pathogenic Neisseria are most likely a result of their localization in different ecological niches.

Molecular analysis of the psa permease complex of Streptococcus pneumoniae

The psaBCA locus of Streptococcus pneumoniae encodes a putative ABC Mn2+-permease complex. Downstream of the operon is psaD, which may be co-transcribed and encodes a thiol peroxidase. Previously, there has been discordance concerning the phenotypic impact of mutations in the psa locus, resolution of which has been complicated by differences in mutant construction and the possibility of polar effects. Here, we constructed unmarked, in frame deletion mutants DeltapsaB, DeltapsaC, DeltapsaA, DeltapsaD, DeltapsaBC, DeltapsaBCA and DeltapsaBCAD in S. pneumoniae D39 to examine the role of each gene within the locus in Mn2+ uptake, susceptibility to oxidative stress, virulence, nasopharyngeal colonization and chain morphology. The requirement for Mn2+ for growth and transformation was also investigated for all mutants. Inductively coupled plasma mass spectrometry (ICP-MS) analysis provided the first direct evidence that PsaBCA is indeed a Mn2+ transporter. However, this study did not substantiate previous reports that the locus plays a role in choline-binding protein pro-duction or chain morphology. We also confirmed the importance of the Psa permease in systemic virulence and resistance to superoxide and hydrogen peroxide, as well as demonstrating a role in nasopharyngeal colonization for the first time. Further evi-dence is provided to support the requirement for Mn2+ supplementation for growth and transformation of DeltapsaB, DeltapsaC, DeltapsaA, DeltapsaBC, DeltapsaBCA and DeltapsaBCAD mutants. However, transformation, as well as growth, of the DeltapsaD mutant was not dependent upon Mn2+ supplementation. We also show that, apart from sensitivity to hydrogen peroxide, the DeltapsaD mutant exhibited essentially similar phenotypes to those of the wild type. Western blot analysis with a PsaD antiserum showed that deleting any of the genes upstream of psaD did not affect its expression. However, we found that deleting psaB resulted in decreased expression of PsaA relative to that in D39, whereas deleting both psaB and psaC resulted in at least wild-type levels of PsaA.

Inhibition of interferon signaling by the New York 99 strain and Kunjin subtype of West Nile virus involves blockage of STAT1 and STAT2 activation by nonstructural proteins

The interferon (IFN) response is the first line of defense against viral infections, and the majority of viruses have developed different strategies to counteract IFN responses in order to ensure their survival in an infected host. In this study, the abilities to inhibit IFN signaling of two closely related West Nile viruses, the New York 99 strain (NY99) and Kunjin virus (KUN), strain MRM61C, were analyzed using reporter plasmid assays, as well as immunofluorescence and Western blot analyses. We have demonstrated that infections with both NY99 and KUN, as well as transient or stable transfections with their replicon RNAs, inhibited the signaling of both alpha/beta IFN (IFN-alpha/beta) and gamma IFN (IFN-gamma) by blocking the phosphorylation of STAT1 and its translocation to the nucleus. In addition, the phosphorylation of STAT2 and its translocation to the nucleus were also blocked by KUN, NY99, and their replicons in response to treatment with IFN-alpha. IFN-alpha signaling and STAT2 translocation to the nucleus was inhibited when the KUN nonstructural proteins NS2A, NS2B, NS3, NS4A, and NS4B, but not NS1 and NS5, were expressed individually from the pcDNA3 vector. The results clearly demonstrate that both NY99 and KUN inhibit IFN signaling by preventing STAT1 and STAT2 phosphorylation and identify nonstructural proteins. responsible for this inhibition.