Human regulatory protein Ki-1/57 has characteristics of an intrinsically unstructured protein

The human protein Ki-1/57 was first identified through the cross reactivity of the anti-CD30 monoclonal antibody Ki-1; in Hodgkin lymphoma cells. The expression of Ki-1/57 in diverse cancer cells and its phosphorylation in peripheral blood leukocytes after mitogenic activation suggested its possible role in cell signaling. Ki-1/57 interacts with several other regulatory proteins involved in cellular signaling, transcriptional regulation and RNA metabolism, suggesting it may have pleiotropic functions. In a previous spectroscopic analysis, we observed a low content of secondary structure for Ki-1/57 constructs. Here, Circular dichroism experiments, in vitro RNA binding analysis, and limited proteolysis assays of recombinant Ki-1/57(122-413) and proteolysis assays of endogenous full length protein from human HEK293 cells suggested that Ki-1/57 has characteristics of an intrinsically unstructured protein. Small-angle X-ray scattering (SAXS) experiments were performed with the C-terminal fragment Ki-1/57(122-413). These results indicated an elongated shape and a partially unstructured conformation of the molecule in solution, confirming the characteristics of an intrinsically unstructured protein. Experimental curves together with ab initio modeling approaches revealed an extended and flexible molecule in solution. An elongated shape was also observed by analytical gel filtration. Furthermore, sedimentation velocity analysis suggested that Ki-1/57 is a highly asymmetric protein. These findings may explain the functional plasticity of Ki-1/57, as suggested by the wide array of proteins with which it is capable of interacting in yeast two-hybrid interaction assays.

Skolmisslyckande - en fråga om vårt samhälles syn på människors värde? : Normer och värderingar i svensk skolmiljö

Denna didaktiskt inriktade C-uppsats i etik tar upp elevers skolmisslyckande satt i förhållande till skolans styrdokument, lärares yrkesetik samt vårt samhälles syn på människors värde. Syftet med uppsatsen är att utifrån Iris Marion Youngs teori kring förtryck samt Harald Ofstads teori kring styrka och svaghet, ur ett etiskt/moraliskt perspektiv, söka efter svar på följande frågeställningar: Kan elever i svensk skola anses vara förtryckta enligt Youngs teori om förtryck? Kan man inom svensk skola finna tendenser till ett sådant förakt för svaghet som Ofstad diskuterar? Vilka bakomliggande strukturella förklaringar kan man utifrån dessa teorier finna till varför vissa lärare i svensk skola medvetet eller omedvetet orsakar vissa elevers känsla av att vara kränkta, misslyckade och osynliggjorda såsom uppsatsens källmaterial påvisar? Metoden består av en etisk analys av kvalitativt empiriskt källmaterial. Källmaterialet, som bygger på delar av en doktorsavhandling i filosofi författad av Carina Henriksson, analyseras med hjälp av analysverktyg skapade utifrån Youngs teori kring förtryck samt Ofstads teori kring styrka och svaghet. Slutsatsen man kan dra utifrån analyserna visar att vissa elever i svensk skola kan anses vara förtryckta samt att man inom svensk skola kan finna tendenser till förakt för svaghet. Analyserna visar även att de bakomliggande strukturella förklaringar man kan finna till detta förtryck samt förakt för svaghet handlar om traditioner som genom historiens gång blivit allmänt vedertagna och därmed normativa, trots att de motsäger de etiska principer vårt samhälle sägs bygga på, principer man kan finna i bland annat skolans värdegrund samt lärares yrkesetiska principer.

Utmaningen från andra berättelser : En studie om moraliskt omdöme, utvidgat tänkande och kritiskt reflekterande berättelser i dialogbaserad feministisk etik

The present study has a threefold aim: First, the theoretical aim is to give a contribution to refinement of the theory of dialogue based feminist ethics, concerning the understanding of judgment and narration within such an ethics.  The study also has an empirical aim, defined as to clarify what kind of knowledge, relevant to the moral judgment of an engaged outsider actor, can be received from dialogical interpretation and analysis of a limited selection of critically reflecting life stories. Third, a methodological aim is defined as to develop an approach to interpretation and analysis of reflecting life stories, which renders the storyteller visible as a reflecting moral subject, and makes the story accessible as a source of knowledge for the moral judgment of an engaged outsider actor. The thesis combines philosophical reflection and argumentation, with a narrative-hermeneutic method for interpretation of life stories, relating the two to each other in a hermeneutic process.  The theoretical reflection draws on Seyla Benhabibs theory of communicative ethics. A dialogue based model for moral justification and a likewise dialogue based model for political legitimacy are at the heart of this universalistic theory, although in combination with a conception of a narratively and hermeneutically constituted context sensitive moral judgment, based on Hannah Arendt’s concept “enlarged thought”. In the reflection, this model is related to other feminist theorizing within the tradition of dialogue based feminist ethics, as found in the works of Iris M. Young, Georgia Warnke and Shari Stone-Mediatore. The empirical study draws on three critically reflecting life stories from Israeli-Palestinian women activists for a just peace. The methodology for interpretation and analysis that is worked out combines dialogical interpretation as presented in Arthur W. Frank’s socio-narratology with a method for structural analysis derived from Shari Stone-Mediatores theory of storytelling as an expression of political resistance struggle. The results show that some stories drawing on marginalized experiences have a potential­ to stimulate further public debate through their capacity to enable a stereoscopic seeing, elucidating a tension between ideologically structured discourse and non-linguistic experience; implying that narrative-hermeneutic competence should be considered crucial for public debate.  

Imagens ecocardiográficas fetais integradas a banco de dados

Este trabalho discorre no escopo de informática médica, no âmbito da Unidade de Cardiologia Fetal do Instituto de Cardiologia - Fundação Universitária de Cardiologia do RS. Sabe-se que a medicina gera um grande volume de dados, sejam eles, textuais, numéricos, gráficos ou mesmo imagens ou sons geradas por equipamentos de ultra-som, tomógrafos computadorizados, ressonância magnética, RX, entre outros. Este trabalho desenvolve a integração das imagens ecocardiográficas fetais ao banco de dados. Atualmente, a tendência observada no desenvolvimento de sistemas de informações é a utilização de banco de dados que sejam capazes de manipular informações completas sobre seus pacientes, tais como: consultas, medicamentos, internações, bem como os laudos de exames com suas respectivas imagens quando estes possuírem. É com base nestas tendências que foram definidos os tópicos relevantes a serem estudados e implementados neste trabalho, integrando os estudos ecocardiográficos fetais com as informações do banco de dados da unidade de cardiologia fetal (UCF). Neste trabalho está apresentado o modelo do banco de dados da UCF. Para esta modelagem foram realizados estudos para aquisição de conhecimento da área e também para compreender as necessidades da unidade Da mesma forma, as imagens ecocardiográficas fetais foram estudadas para que fosse possível serem modeladas junto ao banco de dados. Para esta modelagem foi necessário fazer uma breve revisão dos conceitos utilizados pelo paradigma de orientação a objetos, uma vez que o modelo foi desenvolvido utilizando esta metodologia. As imagens ecocardiográficas fetais receberam grande atenção, uma vez que para elas foram criadas classes distintas. Também para aumentar a funcionalidade foram estudados conceitos de imagem digital, para posterior aplicação sobre as imagens do domínio. Foram realizados estudos sob manipulação de imagens, como modificação do brilho, medidas, filtros e formas de armazenamento. Considerando os formatos de gravação, dois padrões foram contemplados neste trabalho: o utilizado pela placa disponível no instituto denominado DT-IRIS e o DICOM que é um padrão internacional de armazenamento e comunicação de imagens médicas. Por fim, a implementação do protótipo procura demonstrar a viabilidade do modelo proposto, disponibilizando dados textuais, imagens e ainda realizando manipulações sobre estas imagens do domínio.

Sinalização rápida por testosterona na membrana plasmática da célula de sertoli : captação de 45Ca2+ e efeitos eletrofisiológicos

Resumo não disponível.

Construção de um instrumento para aferição da resistência a frustração

Esta tese descreve a fundamentação' e a elaboração de um novo instrumento psicológico, cujo conteúdo textual é a parte integrante deste trabalho. ·Trata-se de instrumento de diagnóstico psicológico referente à resistência à frustração, aferida em duas dimensões como grau de ajustamento e de agressividade. A fundamentação teórica foi apresentada em dois níveis: em termos abrangentes envolvendo todas as teorias sobre agressividade e em termos restritos, ou seja, voltando-se para as teorias que tratam da relação frustração-agressão. Em termos abrangentes (Cap. I, desta tese), considerou-se em 1º lugar, a existência de uma perspectiva disposicional da agressividade, podendo esta ser inata ou aprendida; sob este ângulo foram apreciadas, ainda que sucintamente, as mais importantes teorias sobre o assunto. Em segundo lugar a colocação de uma função reacional atribuída ao comportamento agressivo. . Em termos restritos (Cap. lI), esta última colocação passou a ser o cerne da fundamentação aqui adotada, como relação existente entre frustração e agressão. As principais perspectivas teóricas que alicerçaram este trabalho são as de Dollard e Miller e de S. Rosensweig e E. Allen. Em parte foram utilizados subsídios provenientes das colocações de N.R. F. Maier, de Amsel. Em seguida foi descrita a pesquisa realizada para transformar estes dados teóricos num contexto instrumental objetivo (Cap. 111). Finalmente, o próprio instrumento em sua forma final ê reportado textualmente (Cap. IV). Através deste trabalho V1sa-se poder oferecer uma contribuição inicial dentro de um estudo planejado em termos mais abrangentes, 'uma vez que tentou-se construir um instrumento inicial para uma pesquisa sobre expressividade, espontaneidade e criatividade, como função terapêutica individual e social.

The effects of trust in Brazilian PNPs: interpersonal and interorganizational trust in the cultural sector social organizations in São Paulo, Brazil

This study seeks to evaluate how enterorganizational and interpersonal trust affects the degree of State interference in the operations of public-nonprofit partnerships (PNPs). We conducted a qualitative case study in two Brazilian PNPs, Projeto Guri and Orquestra Sinfônica do Estado de São Paulo, through documental analysis and semi-structured interviews. Content analysis of the data yielded a trust framework that begins to explain how a variety of factors, including the protective qualities of the management contract and the strength of the board, moderate the relationship between interpersonal and interorganizational trust in PNPs. The study reveals that unlike Zaheer et al (1998), interpersonal trust had a unique and prominent effect on State interference and types of collaboration in PNPs. Parting from the suggestions by previous authors to contextualize PNP literature findings, the framework takes into account the highly personalistic qualities of Brazilian culture as well as historical and institutional context while highlighting the crucial role of interpersonal trust in Brazilian PNPs.

Conexões e tensões entre representação e participação social: a elaboração de políticas culturais na democracia brasileira

Esta dissertação pretende examinar a relação entre democracia representativa (eleitoral) e democracia participativa, assim como os diferentes graus de influência da participação, direta ou indireta através de representantes, de atores e grupos socioculturais, antes excluídos, em espaços deliberativos, especialmente em conferências, conselhos, seminários e fóruns abertos para a elaboração de políticas públicas. Para tanto, o trabalho baseia-se na discussão sobre a crise da representação política e a relação com a participação social desenvolvida no âmbito da teoria democrática contemporânea e são utilizados aspectos extraídos da literatura sobre a elaboração de políticas públicas, o conceito de capital social de Pierre Bourdieu e a ideia de democracia comunicativa de Iris Young. A metodologia do trabalho consiste em três estudos de caso interligados: o nacional, relativo à participação em espaços deliberativos e à representação político-eleitoral principalmente para elaboração do Plano Nacional de Cultura; o estadual, relativo à representação política e participação para elaboração de políticas de cultura em Pernambuco; e um caso municipal, a análise da participação em espaços deliberativos abertos para a elaboração do plano e de políticas de cultura no município de Recife.

Impacto das regulamentações no sistema financeiro : uma análise sobre Basiléia e seus custos implícitos no Brasil

A pesquisa visa entender o impacto das regulamentações no sistema financeiro considerando as teorias sobre ciclos econômicos e contextualizando para a implementação de Basileia 3 no mercado brasileiro em 2013. Para desenvolver foram analisados modelo teórico e teste via simulação no programa NETLOGO, considerando-se o numero de instituições financeiras no mercado brasileiro alterando as restrições e colhendo os resultados gráficos. A abordagem teórica para melhor entender os impactos das regulamentações foi feita através da análise da bibliografia disponível sobre Hipótese da Instabilidade Financeira. Em linha com a literatura abordada, há evidências do impacto negativo das regulamentações em sistemas que apresentam choques negativos de produtividade, ainda levando-se em consideração a capacidade das instituições financeiras, no que diz respeito a estrutura de seus balanços para suportarem tais eventos.

Prevalence of biomicroscopic findings in the anterior segment and ocular adnexa among schoolchildren in Natal/Brazil

Objective: To determine the prevalence of ocular findings of the external structures and anterior segment of the eye, detected by biomicroscopic examination in schoolchildren in Natal (RN) - Brazil. Methods: After previous random selection, 1,024 pupils from elementary and secondary public and private schools in the city of Natal were evaluated from March to June 2001. All were submitted to preestablished standard research norms, consisting of identification, demographic information, ophthalmologic biomicroscopic examination, with slit lamp, performed by ophthalmologists from the “Onofre Lopes” University Hospital. Results: Alterations of the conjunctival and palpebral conditions were the most prevalent (10.4% and 6.2% respectively). Follicles (4.2%) and papillae (3.0%) were the frequent conjunctival lesions, while blepharitis (3.5%) and meibomitis (1.1%) were the most detected abnormalities in the eyelids. Upon examining the cornea, iris, lens and anterior vitreous, the most encountered findings were nubecula (0.5%), papillary membrane reliquiae (0.5%), posterior capsula opacity (0.8%) and hyaloid arteria reliquiae (2.0%). Conclusion: The most prevalent findings affecting the external structures of the eye such as eyelids and conjunctiva, consisted of blepharitis followed by follicular reaction of the conjunctiva. The most prevalent abnormalities in the cornea, iris, lens and anterior vitreous were nubecula, papillary membrane reliquiae, posterior capsular opacity and hyaloid arteria reliquiae, in that order

Política pública e educação digital no ensino fundamental em Natal/RN: análise de eficácia da atuação dos objetivos do Proinfo Municipal

The present work turns on the public politics of digital inclusion and of the impact of the Technologies of the Information and Communication in the formation of the citizenship. For this, it was opted for doing an analysis of effectiveness of the performance of the objectives of the Proinfo (National Program of Computer Science in the Education) municipal district of Natal-RN. As instrument of collection of data, three types of questionnaires were used. The collected data were analyzed and discussed starting from it analyzes it descriptive of averages and analyze of multiple regression. The results of the research pointed that the teachers and the students use the computer science in a restricted way. On the other hand, the digital education is not still totally contemplated in the schools beneficiaries' digital rérumé by Proinfo municipal and thus, the inefficacy of the program was verified with relationship the educational training and the students' digital inclusion

Utilização de um ambiente virtual de aprendizagem: um estudo na perspectiva da prática docente

Advances related to information technology are visible and inherent to the management of contemporary organizations, regardless of industrial action. Synchronized with this dynamic, educational institutions are incorporating technological tools that assist its management and academic support to teachers in teaching and interaction with the students. Given that technological innovations are not always taken homogeneously and with the same degree of coverage, remain current and relevant studies on how these technologies are being used in academia. The objective of this research is to identify the usage profile of the functionality of a virtual learning environment related to teaching (undergraduate or postgraduate), demographic variables (age and gender) and institutional (time of admission and academic center of origin.) The methodology applied to the study is descriptive and quantitative. The research is characterized as census, covering all 2152 teachers of undergraduate and graduate students of the Federal University of Rio Grande do Norte, Brazil, who accessed the virtual classes of the Integrated Management of Academic Activities. The study findings revealed that there is a statistically significant difference regarding the use of these tools to teachers who work with undergraduate (49.3%) compared to graduate (6.6%). Regarding gender, women (40.1%) use the system more than men (38.5%). It was also observed that the younger teachers, aged 37 years, are the most active users (42.5%) of the Virtual Class with respect to their elders. For teachers with up to three years time of admission to the UFRN, the pattern of use is more advanced than those with more seniority, as well as the faculty of the Center for Science and Technology are the least likely to use the tools available in relation to other academic centers. It is hoped that with this study managers can direct actions to improve and expand the use of this environment by teachers

Situações de Risco, Trabalho e Saúde de Imigrantes Brasileiros: representações sociais

This study searches in approaching diverse pertinent aspects to the immigration process that affects the countries of the European Union especially in Portugal. The works deriving from this research has its objective in: analyzing the risk, work and health in Brazilian immigrants residing in Lisbon, Portugal. As methodological way, we use the instruments: Test of Free Association of Words and half-structuralized interview. For in such a way, they had been processed in software s for analysis between them: SPSS 14,5, Evoc, Trideux and Alceste. The not-boarded results in this study will be worked as clippings and will be sent for posterior publication. Thus, I consider this work enriching, in view of contributing of the same as mechanisms of understanding of being a immigrant and the possibility of the mobilization of the society and the academic environment for a phenomenon growing each time more, especially, the professionals of health, so we can intervine in a more necessary form within the factors that affects this population layer directly

Repercussões psico-sociais da síndrome dos ovários policísticos: estudo quali-quantitativo

BACKGROUND: Polycystic ovary syndrame (PCOS) has been shown to cause a reduction in quality of life. Little is known on the psychosocial aspects associated with PCOS, especially on women s own experiences regarding the syndrome. Therefore, the aim of this study was to analyze the psychosocial impact of PCOS by measuring the health-related quality of life and exploring the women s own experiences of PCOS. METHODS: A two-phase model of combination of quantitative and qualitative research approaches was conducted, a crass-sectional survey to pravide quantitative data on the quality of life and a qualitative approach to understand the women s experience in relation to the PC OS sympt?ms. For the first phase, 109 women with PC OS and 104 healthy contrais were evaluated with the 36-item short-form health survey (SF-36). Additionally, semi-structured interviews (n=30) were conducted to facilitate in-depth exploration of PC OS women s experience. RESUL TS: PCOS patients showed significant reductions in almost ali SF-36 domains of quality of life when compared with healthy contrais (physical function 76.5:!:20.5 and 84.6:!:15.9, respectively; physical rale function 56.4:!:43.3 and 72.6:!:33.3; general health 55.2:!:21.0 and 62.5:!:17.2; vitality 49.6:!:21.3 and 55.3:!:21.3; social function 55.3:!:32.4 and 66.2:!:26.7; emotional rale function 34.2:!:39.7 and 52.9:!:38.2; mental health 50.6:!:22.8 and 59.2:!:20.2). Thematic analysis revealed reports of feeling abnormal , sadness, afraid and anxiety. These feelings were related to four symptoms commonly experienced by women with PCOS: excess hair grawth; irregular or absent menstruation, infertility and obesity. CONCLUSIONS: Polycystic ovary syndrame impacts women both physically and psychosocially. Owing to this, women with PCOS need not only medical treatment for. the reproductive, esthetic and metabolic repercussions, but also the care of a multidisciplinary team, since PCOS is not just a physical condition, but also an important psychosocial problem that affects various aspects of a woman s life