989 resultados para Viés de seleção
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Pós-graduação em Agronomia - FEIS
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This article seeks to understand the production of the modern school in Brazil and Portugal at the turn of the 20th century. The analysis falls on the pedagogical knowledge constituted around school programs disseminated in pedagogical manuals at the Normal Schools in Brazil and Portugal. The study uses three teacher training manuals as source of information: Curso pratico de pedagogia destinado aos alunos-mestres das escholas normaes primarias e aos instituidores em exercício (1874), by Mr. Daligault; Elementos de Pedagogia para servirem de guia aos candidatos ao magistério primário (1870), by José Maria da Graça Affreixo and Henrique Freire; and Lições de metodologia (1920), by Bernardino da Fonseca Lage.
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Pós-graduação em Ciências Biológicas (Microbiologia Aplicada) - IBRC
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Pós-graduação em Design - FAAC
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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV
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Pós-graduação em Desenvolvimento Humano e Tecnologias - IBRC
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Pós-graduação em Agronomia (Proteção de Plantas) - FCA
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Currently, mammalian cells are the most utilized hosts for biopharmaceutical production. The culture media for these cell lines include commonly in their composition a pH indicator. Spectroscopic techniques are used for biopharmaceutical process monitoring, among them, UV–Vis spectroscopy has found scarce applications. This work aimed to define artificial neural networks architecture and fit its parameters to predict some nutrients and metabolites, as well as viable cell concentration based on UV–Vis spectral data of mammalian cell bioprocess using phenol red in culture medium. The BHK-21 cell line was used as a mammalian cell model. Off-line spectra of supernatant samples taken from batches performed at different dissolved oxygen concentrations in two bioreactor configurations and with two pH control strategies were used to define two artificial neural networks. According to absolute errors, glutamine (0.13 ± 0.14 mM), glutamate (0.02 ± 0.02 mM), glucose (1.11 ± 1.70 mM), lactate (0.84 ± 0.68 mM) and viable cell concentrations (1.89 105 ± 1.90 105 cell/mL) were suitably predicted. The prediction error averages for monitored variables were lower than those previously reported using different spectroscopic techniques in combination with partial least squares or artificial neural network. The present work allows for UV–VIS sensor development, and decreases cost related to nutrients and metabolite quantifications.
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As celulases atualmente são enzimas extensivamente estudadas para a hidrólise de resíduos lignocelulósicos para obtenção de açúcares fermentescíveis utilizáveis em diferentes processos biotecnológicos, como a produção de bioetanol. O objetivo deste trabalho foi encontrar fungos celulolíticos que sejam eficientes nos processos de degradação da biomassa lignocelulósica. No presente trabalho foram selecionados 4 fungos celulolíticos previamente isolados pelo Laboratório de Biotecnologia Industrial (UNESP – Assis). Estes fungos foram cultivados em bagaço de cana-de-açúcar como substrato e para seus extratos enzimáticos foram testados suas atividades para celulases (FPase) e endoglucanases (CMCase). Os fungos FS09 (0,054 FPU/mL e 1,79 FPU/g de substrato, 0,874 U/mL e 29,1 U/g de substrato) e M51 (0,049 FPU/mL e 1,62 FPU/g de substrato, 1,094 U/mL e 36,46 U/g de substrato), mostrando valores superiores ao fungo T.reesei CCT 2768 que é referência em estudos e produção de celulases.
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Pós-graduação em Agronomia (Proteção de Plantas) - FCA
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Pós-graduação em Microbiologia - IBILCE
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Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV
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Pós-graduação em Genética e Melhoramento Animal - FCAV
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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The objective of this study was to assess families and highlight the superior progenies of sugarcane originating from 38 biparental crosses for the following attributes: tons of cane per hectare (TCH), tons of biomass per hectare (TBIOH), brix (% cane juice), fiber content, purity, pol and total recoverable sugar (TRS). The data were analyzed by mixed model REML / BLUP in the REML (Restricted Maximum Likelihood) allowed us to estimate genetic parameters and BLUP (best linear unbiased prediction) to predict the additive and genotypic values. The best family for the attributes TCH and TBIOH was 41, whose parents are cultivars IACSP022019 x CTC9. In individual selection for TCH, the plant number 3 of Block 2, the crossing 78, showed the best results. To TBIOH the plant number 33, Block 1, family 41, showed the best results. Families 40, 41, 43, 68, 69, 79, 91, 92 and 147, were higher for the variables brix, pol, purity, and ATR, where as 85 families, 147, 148, 149, 161, 163, 177, 178, 179, and 183 were higher for fiber. The family 147 whose parents are IACSP042286 x IACSP963055, showed three progenies ranked among the top ten for both brix, and for fiber, which identifies the combination as a potential source of progenies for bioenergy production.