Isolation of urease-positive Vibrio parahaemolyticus from diarrheal patients in Northeast Brazi

Of 21 human fecal strains of Vibrio parahaemolyticus, isolated on the Northeast Coast of Brazil, eight (38%) were urease positive. Most of these strains, in contrast to the urease-negative ones, did not produce the hemolysin responsible for the Kanagawa phenomenon.

Víbrios não coléricos na rotina enterobacteriológica

De 3.250 fezes diarréicas, recebidas para diagnóstico microbiológico em laboratório clínico particular, no Recife, Brasil, isolaram-se 55 (1,7%) linhagens de Vibrio. O estudo foi realizado entre maio de 1989 e maio de 1991. Para o isolamento de Vibrio, os espécimes fecais foram enriquecidos em água peptonada alcalina suplementada com 2% de NaCl e subcultivados em ágar tiosulfato-citrato-sais biliares-sacarose (TCBS). Das espécies isoladas, V. parahaemolyticus foi a mais freqüente (24 cepas), seguida de V. furnissii (15 cepas), V. cholerae não 01 (6 cepas), V. alginolyticus (4 cepas), V. fluvialis (2 cepas) e Vibrio sp. (1 cepa). Do ponto de vista custo-benefício, a baixa taxa de isolamento de Vibrio levanta dúvidas acerca da utilidade do TCBS na rotina enterobacteriológica dos laboratórios clínicos

A performatividade dos movimentos sociais contemporâneos: dissertação

Dissertação submetida à Escola Superior de Teatro e Cinema para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Teatro - especialização em Teatro e Comunidade.

Vibrios among patients of good socioeconomic conditions during the cholera epidemic in Recife, Brazil

Between March and July, 1992, we screened for Vibrio all fecal samples submitted for bacteriologic diagnosis at a private clinical laboratory in Recife. Of 1435 cultures examined only 1 (0.07%) was positive for V.cholerae 01, biovar Eltor, serovar Inaba, but 17 (1.2%) yielded non-cholera Vibrio (V.cholerae non-01; V.fluvialis; V.furnissii, V.parahaemolyticus and Vibrio spp). Thus, V.cholerae 01, differently of other enteropathogenic vibrios, spared individuals of good socioeconomic conditions even during the cholera epidemic, which made hundreds of victims in the neighboring slums.

Circulation of Eastern equine encephalitis, Western equine encephalitis, Ilhéus, Maguari and Tacaiuma viruses in equines of the Brazilian Pantanal, South America

Neutralizing antibodies to EEE (6.7%), WEE (1.2%), ILH (26.6%), MAG (28.2%) and TCM (15.7%) viruses were found in sera of 432 equines of the Brazilian Pantanal, area where undiagnosed horse deaths are frequently observed. A 4-fold rise in CF titer to EEE virus was detected in acute and convalescent sera of an encephalitis horse sacrified in 1992. Antibodies to EEE, ILH, MAG and TCM viruses were detected in horses less than 2 years old indicating recent circulation of these viruses in the Pantanal. The evidence of recent equine encephalitis associated with rising CF titer to EEE warrants a more intensive study with attempts to isolate virus from horses with clinical manifestations of encephalitis.

Suporte técnico à produção e comercialização de produtos cosméticos e detergentes

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para obtenção de grau de Mestre em Engenharia Química e Bioquímica

Malaria serology: performance of six Plasmodium falciparum antigen extracts and of three ways of determining serum titers in IgG and IgM-ELISA

The study evaluated six Plasmodium falciparum antigen extracts to be used in the IgG and IgM enzyme-linked immunosorbent assays (ELISA), for malaria diagnosis and epidemiological studies. Results obtained with eighteen positive and nine negative control sera indicated that there were statistically significant differences among these antigen extracts (Multifactor ANOVA, p< 0.0001). Urea, sodium deoxycholate and Zwittergent antigen extracts performed better than did the three others, their features being very similar for the detection of IgG antibodies. Urea, alkaline and sodium deoxycholate antigen extracts proved to be better than the others for the detection of IgM antibodies. A straight line relationship was found between the optical densities (or their respective log 10) and the log 10 of antibody dilutions, with a very constant slope. Thus serum titers could be determined by direct titration and by two different equations, needing only one serum dilution. For IgM antibody detections, log 10 expression gave results that better correlated with direct titration (95% Bonferroni). For IgG antibody detections, the titer differences were not significant. The reproducibility of antibody titers and antigen batches was also evaluated, giving satisfactory results.

Água e energia: conexões para uma nova sustentabilidade

Comunicação apresentada no VIII Congresso Nacional de Geografia na Reitoria da Universidade de Lisboa, a 27 de outubro de 2011

Trichosporon beigelii peritonitis associated with continuous ambulatory peritoneal dialysis

This paper reports a case of peritonitis caused by Trichosporon beigelii in a woman submitted to continuous ambulatory peritoneal dialysis. Diagnosis was established by direct examination and culture of dialysis effluent.

Nunca mais é sexta-feira: o impacto do processo de recuperação de recursos durante o fim de semana no engagement

Orientação: Doutora Maria Alexandra Pacheco Ribeiro da Costa

Bibliotecas da Administração Central do Estado - Que futuro(s)?

Estudo realizado por solicitação do INA. Concluído e entregue em outubro de 2015.

Induction of iron regulated proteins during normal growth of Neisseria meningitidis in a chemically defined medium

The expression of iron regulated proteins (IRPs) in vitro has been obtained in the past by adding iron chelators to the culture after bacterial growth, in the presence of an organic iron source. We have investigated aspects concerning full expression of the meningococcal IRPs during normal growth, in defined conditions using Catlin medium, Mueller Hinton and Tryptic Soy Broth (TSB). The expression of IRPs varied between different strains with respect to Ethylenediamine Di-ortho-Hidroxy-phenyl-acetic acid (EDDA) concentrations, and according to culture medium, and also between different lots of TSB. For each strain, a specific set of IRPs were expressed and higher EDDA concentrations, or addition of glucose, or use of different culture media did not resulted in a differential expression of IRPs. We were not able to grow N. meningitidis under normal growth conditions using Desferal. We looked for a good yield of outer membrane vesicles (OMVs) expressing IRPs in iron-deficient Catlin medium containing EDDA and Hemin. Culture for 32 h at 30ºC after growing for 16 h at 37ºC supported good bacterial growth. Bacterial lysis was noted after additional 24 h at 30ºC. Approximately 4 times more OMVs was recoverable from a culture supernatant after 24 h at 30ºC than from the cells after 16 h at 37ºC. The IRP were as well expressed in OMVs from culture supernatant obtained after 24 h at 30ºC as from the cells after 16 h at 37ºC.

Coluna de som utilizando array de transdutores de ultrasons

Trabalho Final de Mestrado para obtenção do grau de Mestre em Engenharia de Electrónica e Telecomunicações

A Problemática Ambiental. Políticas e Medidas para a Conservação dos Ecossistemas em São Tomé

Dissertação de Mestrado em Ciência Política e Relações Internacionais Área de especialização: Globalização e Ambiente

A importância das consultoras no processo de internacionalização das micro, pequenas e médias empresas em Portugal

Relatório de Estágio Apresentado ao Instituto de Contabilidade e Administração do Porto para a obtenção do grau de Mestre em Empreendedorismo e Internacionalização, sob orientação da Mestre Inês Veiga Pereira