Flora polínica da Reserva do Parque Estadual das Fontes do Ipiranga (São Paulo, Brasil): família: 135-Asclepiadaceae

No presente trabalho, foram estudados os polinários de nove gêneros e 16 espécies de Asclepiadaceae ocorrentes na Reserva do Parque Estadual das Fontes do Ipiranga: Asclepias curassavica L., Blepharodon nitidum (Vell.) J F. Macbr., Ditassa burchellii var. vestita (Malme) Fontella, Ditassa hispida (Vell.) Fontella, Ditassa tomentosa (Decne.) Fontella, Gomphocarpus physocarpus E. Mey., Gonianthela axillaris (Vell.) Fontella & E.A. Schwarz, Matelea glaziovii (E. Fourn.) Morillo, Orthosia urceolata E. Fourn., Oxypetalum appendiculatum Mart., Oxypetalum capitatum subsp. capitatum Mart., Oxypetalum insigne (Decne.) Malme, Oxypetalumpachyglossum Decne., Oxypetalumpedicellatum Decne., Oxypetalum wightianum Hook. & Arn., Tassadia subulata var. subulata (Vell.) Fontella & E.A. Schwarz. São apresentadas descrições e ilustrações para todas as espécies estudadas, bem como chaves para identificação das espécies dos gêneros Ditassa e Oxypetalum tomando como base a morfologia dos polinários.

Limitações do paradigma científico hegemônico no acolhimento ao sofrimento psíquico na atenção básica

O problema que motivou a realização do presente trabalho foi a dificuldade em se acolher o sofrimento psíquico nas unidades de atenção básica. Neste trabalho, investigamos se esta dificuldade se deve à inadequação epistemológica do paradigma hegemônico. Realizamos uma revisão de literatura e analisamos a utilização do termo paradigma na produção científica brasileira recente. Após busca pelas palavras-chave paradigma e medicina e seleção prévia, foram escolhidos 17 textos. A análise foi feita em duas etapas: análise linguística e levantamento da temática emergente. Levantamos as cinquenta palavras mais frequentes e as agrupamos em comunidades de coocorrência, utilizando o algoritmo de Clauset. Os textos discutiam as ideias de Kuhn e trouxeram tanto experiências inovadoras já implantadas em unidades de saúde quanto exemplos de mudanças curriculares nos cursos de medicina. A promoção da saúde aparece como um novo e promissor paradigma para a saúde.

Composição e abundância dos caranguejos (Decapoda, Brachyura) nas regiões de Ubatuba e Caraguatatuba, litoral norte paulista, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mass spectrometric characterization of two novel inflammatory peptides from the venom of the social wasp Polybia paulista

The social wasp P. paulista is relatively common in southeast Brazil causing many medically important stinging incidents. The seriousness of these incidents is dependent on the amount of venom inoculated by the wasps into the victims, and the characteristic envenomation symptoms are strongly dependent on the types of peptides present in the venom. In order to identify some of these naturally occurring peptides available in very low amounts, an analytical protocol was developed that uses a combination of reversed-phase and normal-phase high-performance liquid chromatography (HPLC) of wasp venom for peptide purification, with matrix-assisted laser desorption/ionization time-of-flight post-source decay mass spectrometry (MALDI-Tof-PSD-MS) and low-energy collision-induced dissociation (CID) in a quadrupole time-of-flight tandem mass spectrometry (QTof-MS/MS) instrument for peptide sequencing at the sub-picomole level. The distinction between Leu and Ile was achieved both by observing d-type fragment ions obtained under CID conditions and by comparison of retention times of the natural peptides and their synthetic counterparts (with different combinations of I and/or L at N- and C-terminal positions). To distinguish the isobaric residues K and Q, acetylation of peptides was followed by Q-Tof-MS analysis. The primary sequences obtained were INWLKLGKMVIDAL-NH2 (MW 1611.98Da) and IDWLKLGKMVMDVL-NH2 (MW 1658.98Da). Micro-scale bioassay protocols characterized both peptides as presenting potent hemolytic action, mast cell degranulation, and chemotaxis of poly-morphonucleated leukocyte (PMNL) cells. The primary sequences and the bioassay results suggest that these toxins constitute members of a new sub-class of mastoparan toxins, directly involved in the occurrence of inflammatory processes after wasp stinging. Copyright (C) 2004 John Wiley Sons, Ltd.

Structural and functional characterization of two novel peptide toxins isolated from the venom of the social wasp Polybia paulista

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Myotoxic effects of mastoparan from Polybia paulista (Hymenoptera, Epiponini) wasp venom in mice skeletal muscle

In a previous study, we showed that the Polybia paulista wasp venom causes strong myonecrosis. This study was undertaken to characterize the myotoxic potency of mastoparan (Polybia-MPII) isolated from venom (0.25 mu g/mu l) and injected in the tibial anterior (TA) muscle (i.m.) of Balb/c mice. The time course of the changes was followed at muscle degenerative (3 and 24 h) and regenerative (3, 7, and 21 days) periods (n = 6) after injection and compared to matched controls by calculation of the percentage of cross-sectional area affected and determination of creatine kinase (CK) activity (n = 10). The results showed that although NIP was strongly myotoxic, its capacity for regeneration was maintained high. Since the extent of tissue damage was not correlated with the CK serum levels, which remained very low, we raised the hypothesis that the enzyme underwent denaturation by the peptide. Evidence suggested that MP induced the death of TA fibers by necrosis and apoptosis and had the sarcolemma as its primordial target. Given its amphiphilic polycationic nature and based on the vast spectrum of functions attributed to the peptide, we suggest that MP interaction with cell membrane impaired the phosphorylation of dystrophin essential for sarcolemma mechanical stability, and disturbed Ca2+ mobilization with obvious implications on sarcoplasmic reticulum and mitochondrial functioning. (c) 2007 Elsevier Ltd. All rights reserved.

Characterization of two novel polyfunctional mastoparan peptides from the venom of the social wasp Polybia paulista

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Proteomic Characterization of the Hyaluronidase (EC 3.2.1.35) from the Venom of the Social Wasp Polybia paulista

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Hyperalgesic and edematogenic effects of peptides isolated from the venoms of honeybee (Apis mellifera) and neotropical social wasps (Polybia paulista and Protonectarina sylveirae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Polybioside, a Neuroactive Compound from the Venom of the Social Wasp Polybia paulista

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Proteomic characterization of the multiple forms of the PLAs from the venom of the social wasp Polybia paulista

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Profiling the Proteome of the Venom from the Social Wasp Polybia paulista: A Clue to Understand the Envenoming Mechanism

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Structural and functional characterization of N-terminally blocked peptides isolated from the venom of the social wasp Polybia paulista

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Levantamento florístico de Floresta Atlântica no sul do Estado de São Paulo, Parque Estadual Intervales, Base Saibadela

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)