PAX5 activates the transcription of the human telomerase reverse transcriptase gene in B cells.

Telomerase is an RNA-dependent DNA polymerase that synthesizes telomeric DNA. Its activity is not detectable in most somatic cells but it is reactivated during tumorigenesis. In most cancers, the combination of hTERT hypermethylation and hypomethylation of a short promoter region is permissive for low-level hTERT transcription. Activated and malignant lymphocytes express high telomerase activity, through a mechanism that seems methylation-independent. The aim of this study was to determine which mechanism is involved in the enhanced expression of hTERT in lymphoid cells. Our data confirm that in B cells, some T cell lymphomas and non-neoplastic lymph nodes, the hTERT promoter is unmethylated. Binding sites for the B cell-specific transcription factor PAX5 were identified downstream of the ATG translational start site through EMSA and ChIP experiments. ChIP assays indicated that the transcriptional activation of hTERT by PAX5 does not involve repression of CTCF binding. In a B cell lymphoma cell line, siRNA-induced knockdown of PAX5 expression repressed hTERT transcription. Moreover, ectopic expression of PAX5 in a telomerase-negative normal fibroblast cell line was found to be sufficient to activate hTERT expression. These data show that activation of hTERT in telomerase-positive B cells is due to a methylation-independent mechanism in which PAX5 plays an important role.

Resistance to Fusarium dry root rot disease in cassava accessions

The objective of this work was to identify sources of resistance to dry root rot induced by Fusarium sp. in cassava accessions. A macroconidial suspension (20 µL) of 11 Fusarium sp. isolates was inoculated in cassava roots, from 353 acessions plus seven commercial varieties. Ten days after inoculation, the total area colonized by the pathogen on the root pulp was evaluated by digital image analysis. Cluster analysis revealed the presence of five groups regarding resistance. The root lesion areas ranged from 18.28 to 1,096.07 mm² for the accessions BGM 1518 and BGM 556, respectively. The genotypes BGM 1042, BGM 1552, BGM 1586, BGM 1598, and BGM 1692 present the best agronomical traits.

Conformité du langage françois avec le grec. édition accompagnée de notes et précédée d'un Essai sur la vie et les ouvrages de cet auteur ([Reprod.]) / par Henri Estienne... ; par Léon Feugère,...

Collection : Archives de la linguistique française ; 146

Les premices ou Le I livre des proverbes epigrammatizez ou des epigrammes proverbializez... ([Reprod.]) / par Henri Estiene

Collection : Archives de la linguistique française ; 145

Deux dialogues du nouveau langage françois italianizé... ([Reprod.]) / par Henri Estienne ; avec introduction et notes par P. [Paul] Ristelhuber

Collection : Archives de la linguistique française ; 147

Remarques sur les principales difficultez que les estrangers ont en la langue françoise : avec un recueil alphabétique de plusieurs mots... ([Reprod.]) / Alcide de Saint Maurice

Collection : Archives de la linguistique française ; 357

Les agrémens du langage réduits a leurs principes ([Reprod.])

Collection : Archives de la linguistique française ; 170

Growth and functionalization of carbon nanotubes on quartz filter for environmental applications

Background: Air pollution has become an important issue worldwide due to its adverse health effects. Among the different air contaminants, volatile organic compounds (VOCs) are liquids or solids with a high vapor pressure at room temperature that are extremely dangerous for human health. Removal of these compounds can be achieved using nanomaterials with tailored properties such as carbon nanotubes. Methods: Vertically-aligned multiwall carbon nanotubes (CNTs) were successfully grown on quartz filters by means of plasma enhanced chemical vapor deposition (PECVD). Furthermore, a plasma treatment was performed in order to modify the surface properties of the CNTs. The adsorption/desorption processes of three chlorinated compounds (trichloroethylene, 1,2-dichlorobenzene and chloroform) on the CNTs were studied using mass spectrometry measurements with a residual gas analyzer. Results: The adsorption capability of the CNTs increased after functionalization of their surface with a water plasma treatment. In addition, it was found that the presence of aromatic rings, water solubility and polarity of the VOCs play an important role on the adsorption/desorption kinetics at the CNTs surface. Conclusions: This study demonstrates the applicability of CNTs deposited on quartz filters for the removal or selective detection of volatile organic compounds (VOCs). The presence of aromatic rings in VOCs results in π -stacking interactions with a significant increase of their adsorption. On the other hand, it was found that CNTs surface interactions increase with water solubility and polarity of the VOC.

Oeuvres de François de La Mothe Le Vayer,.... Tome 4 / Partie 2

Collection : Archives de la linguistique française ; 210

Oeuvres de François de La Mothe Le Vayer,.... Tome 7 / Partie 2

Collection : Archives de la linguistique française ; 210

Oeuvres de François de La Mothe Le Vayer,.... Tome 2 / Partie 2

Collection : Archives de la linguistique française ; 210

Oeuvres de François de La Mothe Le Vayer,.... Tome 3 / Partie 1

Collection : Archives de la linguistique française ; 210