EPI Update: Special Alert, July 8, 2013

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health. SPECIAL ALERT

Report on special investigation of the Department of Inspections and Appeals, Office of the State Public Defender, for the period August 31, 2007 through March 31, 2011

Report on a special investigation of the State Public Defender’s Office for the period August 31, 2007 through March 31, 2011

EPI Update: Special Alert, July 12, 2013

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health. SPECIAL ALERT

EPI Update: Special Alert, July 19, 2013

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health. SPECIAL ALERT

Report on special investigation of the City of Farmington for the period July 1, 2009 through November 30, 2012

Special investigation of the City of Farmington for the period July 1, 2009 through November 30, 2012

Report on a special investigation of the City of Grand River for the period July 1, 2004 through March 7, 2012

Report on a special investigation of the City of Grand River for the period July 1, 2004 through March 7, 2012

EPI Update: Special Alert, July 31, 2013

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health. SPECIAL ALERT

Report on a special investigation of the football program at Lincoln High School within the Des Moines Independent Community School District for the period May 30, 2003 through October 31, 2012

Report on a special investigation of the football program at Lincoln High School within the Des Moines Independent Community School District for the period May 30, 2003 through October 31, 2012

Molecular phylogenetics of soricid shrews (Mammalia) based on mitochondrial cytochrome b gene sequences: with special reference to the Soricinae

Molecular phylogeny of soricid shrews (Soricidae, Eulipotyphla, Mammalia) based on 1140 bp mitochondrial cytochrome b gene (cytb) sequences was inferred by the maximum likelihood (ML) method. All 13 genera of extant Soricinae and two genera of Crocidurinae were included in the analyses. Anourosorex was phylogenetically distant from the main groupings within Soricinae and Crocidurinae in the ML tree. Thus, it could not be determined to which subfamily Anourosorex should be assigned: Soricinae, Crocidurinae or a new subfamily. Soricinae (excluding Anourosorex) should be divided into four tribes: Neomyini, Notiosoricini, Soricini and Blarinini. However, monophyly of Blarinini was not robust in the present data set. Also, branching orders among tribes of Soricinae and those among genera of Neomyini could not be determined because of insufficient phylogenetic information of the cytb sequences. For water shrews of Neomyini (Chimarrogale, Nectogale and Neomys), monophyly of Neomys and the Chimarrogale-Nectogale group could not be verified, which implies the possibility of multiple origins for the semi-aquatic mode of living among taxa within Neomyini. Episoriculus may contain several separate genera. Blarinella was included in Blarinini not Soricini, based on the cytb sequences, but the confidence level was rather low; hence more phylogenetic information is needed to determine its phylogenetic position. Furthermore, some specific problems of taxonomy of soricid shrews were clarified, for example phylogeny of local populations of Notiosorex crawfordi, Chimarrogale himalayica and Crocidura attenuata.

Addendum to a report on a special investigation of the Iowa Department of Transportation for the period January 29, 1994 through June 30, 2013

Addendum to a report on a special investigation of the Iowa Department of Transportation for the period January 29, 1994 through June 30, 2013

Responses of yield and N use of spring sown crops to N fertilization, with special reference to the use of plant growth regulators

Selostus: Typpilannoituksen ja kasvunsääteiden vaikutukset kevätviljojen ja rypsin satoon sekä typen käyttöön

Report on a special investigation of Remsen-Union Family Connections for the period July 1, 2011 through October 31, 2012

Report on a special investigation of Remsen-Union Family Connections for the period July 1, 2011 through October 31, 2012

Behavior of nuclear matrix proteins during camptothecin-induced apoptosis in HL-60 human leukemia cells.

In this study we focused our attention on the behavior of four nuclear matrix proteins during the various stages of apoptosis in the HL-60 cell line exposed to the DNA topoisomerase I inhibitor, camptothecin. We have examined the following antigens by immunocytochemical techniques: (i) the 180-kDa nucleolar isoform of DNA topoisomerase II; (ii) a 126-kDa polypeptide of nuclear bodies; (iii) a 125-kDa protein; and (iv) a 160-kDa polypeptide which are known to be components of the matrix inner network. Indirect immunofluorescence experiments were performed to follow these nuclear matrix antigens during apoptosis. Moreover, the ultrastructural localization of both 125- and 160-kDa proteins was investigated by electron microscope immunocytochemistry with gold-conjugated secondary antibodies. While the antibody to the nucleolar isoform of DNA topoisomerase II gave a fluorescent pattern that was well-maintained until the late phases of apoptosis, the other three nuclear antigens showed marked modifications in their distribution. A common feature, particularly evident for 125- and 160-kDa proteins, was their absence from cap-shaped chromatin marginations, whereas they were present in the areas of remaining decondensed chromatin. The 126-kDa polypeptide concentrated progressively in an irregular mass at the opposite side of the crescentic caps and then broke up in fine spots. The 125- and 160-kDa proteins localized in the nucleolus and precisely within certain granules which are known to appear in the nucleolar area after camptothecin administration. These results show that, in addition to the well-known chromatin changes, nuclear organization undergoes other rearrangements during the apoptotic process.