Report on the Iowa Braille and Sight Saving School, Vinton, Iowa, for the year ended June 30, 2007

Report on the Iowa Braille and Sight Saving School, Vinton, Iowa, for the year ended June 30, 2007

Report on the Iowa School for the Deaf, Council Bluffs, Iowa for the year ended June 30, 2007

Report on the Iowa School for the Deaf, Council Bluffs, Iowa for the year ended June 30, 2007

Review of the Sigourney Community School District for the period July 1, 2006 through February 29, 2008

Review of the Sigourney Community School District for the period July 1, 2006 through February 29, 2008

Special investigation of selected transactions at Perry High School for the period July 1, 2005 through November 30, 2007

Special investigation of selected transactions at Perry High School for the period July 1, 2005 through November 30, 2007

Report on a special investigation of the West Marshall Community School District for the period January 1, 2001 through June 30, 2007

Report on a special investigation of the West Marshall Community School District for the period January 1, 2001 through June 30, 2007

Identification of C(4)-dicarboxylate transport systems in Pseudomonas aeruginosa PAO1.

Pseudomonas aeruginosa utilizes preferentially C(4)-dicarboxylates such as malate, fumarate, and succinate as carbon and energy sources. We have identified and characterized two C(4)-dicarboxylate transport (Dct) systems in P. aeruginosa PAO1. Inactivation of the dctA(PA1183) gene caused a growth defect of the strain in minimal media supplemented with succinate, fumarate or malate, indicating that DctA has a major role in Dct. However, residual growth of the dctA mutant in these media suggested the presence of additional C(4)-dicarboxylate transporter(s). Tn5 insertion mutagenesis of the ΔdctA mutant led to the identification of a second Dct system, i.e., the DctPQM transporter belonging to the tripartite ATP-independent periplasmic (TRAP) family of carriers. The ΔdctA ΔdctPQM double mutant showed no growth on malate and fumarate and residual growth on succinate, suggesting that DctA and DctPQM are the only malate and fumarate transporters, whereas additional transporters for succinate are present. Using lacZ reporter fusions, we showed that the expression of the dctA gene and the dctPQM operon was enhanced in early exponential growth phase and induced by C(4)-dicarboxylates. Competition experiments demonstrated that the DctPQM carrier was more efficient than the DctA carrier for the utilization of succinate at micromolar concentrations, whereas DctA was the major transporter at millimolar concentrations. To conclude, this is the first time that the high- and low-affinity uptake systems for succinate DctA and DctPQM have been reported to function coordinately to transport C(4)-dicarboxylates and that the alternative sigma factor RpoN and a DctB/DctD two-component system regulates simultaneously the dctA gene and the dctPQM operon.

How different are returns to education? Evidence from German school choices

This study presents estimates of returns to post-secondary educationand wage differentials among graduates fromdifferent secondary schoolsin Germany. I use an empirical model that captures the basic features ofthe German education system. It controls for selection into post-secondaryeducation and treats latter as endogenous in the wage equation. Myresults show that OLS estimates are severely biased. The direction ofthe bias depends on the secondary school type. Annual returns topost-secondary education differ significantly: they are eight timeshigher for graduates from the highest secondary school than for graduatesfrom the lowest secondary school.

Audit report on Sheldon Community School District in Sheldon, Iowa for the year ended June 30, 2008

Audit report on Sheldon Community School District in Sheldon, Iowa for the year ended June 30, 2008

Audit report on Clarke Community School District in Osceola, Iowa for the year ended June 30, 2008

Audit report on Clarke Community School District in Osceola, Iowa for the year ended June 30, 2008

The rate-limiting step for glucose transport into the hypothalamus is across the blood-hypothalamus interface.

Specialized glucosensing neurons are present in the hypothalamus, some of which neighbor the median eminence, where the blood-brain barrier has been reported leaky. A leaky blood-brain barrier implies high tissue glucose levels and obviates a role for endothelial glucose transporters in the control of hypothalamic glucose concentration, important in understanding the mechanisms of glucose sensing We therefore addressed the question of blood-brain barrier integrity at the hypothalamus for glucose transport by examining the brain tissue-to-plasma glucose ratio in the hypothalamus relative to other brain regions. We also examined glycogenolysis in hypothalamus because its occurrence is unlikely in the potential absence of a hypothalamus-blood interface. Across all regions the concentration of glucose was comparable at a given plasma glucose concentration and was a near linear function of plasma glucose. At steady-state, hypothalamic glucose concentration was similar to the extracellular hypothalamic glucose concentration reported by others. Hypothalamic glycogen fell at a rate of approximately 1.5 micromol/g/h and remained present in substantial amounts. We conclude for the hypothalamus, a putative primary site of brain glucose sensing that: the rate-limiting step for glucose transport into brain cells is at the blood-hypothalamus interface, and that glycogenolysis is consistent with a substantial blood -to- intracellular glucose concentration gradient.

A aprendizagem como o processo de aquisição e construção do conhecimento. O Caso da utilização dos Simuladores no ISCEE Cabo Verde Business School

O objectivo desta comunicação é demonstrar a importância da relação entre modelos de aprendizagem dinâmicos e a criação do conhecimento como mecanismo de transferência e disseminação na sociedade em Cabo Verde, através de práticas pedagógicas utlizadas no ISCEE, como demonstra a utilização dos simuladores. Esta nova abordagem, com a utilização de simuladores em contexto de sala de aula, potencia a interacção entre os estudantes e permite uma rápida troca de informação entre as partes e ao mesmo tempo melhora o processo de tomada de decisão. O processo de aprendizagem através dos simuladores potencia a aquisição e construção do conhecimento e a dinâmica do grupo, em especial a memória transactiva e relacional. Este artigo, demonstra que as novas práticas pedagógicas, com base nas novas tecnologias da comunicação e informação, devem ter o foco no grupo e não no aluno como entidade isolada.

Audit report on Perry Community School District in Perry, Iowa for the year ended June 30, 2008

Audit report on Perry Community School District in Perry, Iowa for the year ended June 30, 2008

The role of SGLT1 and GLUT2 in intestinal glucose transport and sensing.

Intestinal glucose absorption is mediated by SGLT1 whereas GLUT2 is considered to provide basolateral exit. Recently, it was proposed that GLUT2 can be recruited into the apical membrane after a high luminal glucose bolus allowing bulk absorption of glucose by facilitated diffusion. Moreover, SGLT1 and GLUT2 are suggested to play an important role in intestinal glucose sensing and incretin secretion. In mice that lack either SGLT1 or GLUT2 we re-assessed the role of these transporters in intestinal glucose uptake after radiotracer glucose gavage and performed Western blot analysis for transporter abundance in apical membrane fractions in a comparative approach. Moreover, we examined the contribution of these transporters to glucose-induced changes in plasma GIP, GLP-1 and insulin levels. In mice lacking SGLT1, tissue retention of tracer glucose was drastically reduced throughout the entire small intestine whereas GLUT2-deficient animals exhibited higher tracer contents in tissue samples than wild type animals. Deletion of SGLT1 resulted also in reduced blood glucose elevations and abolished GIP and GLP-1 secretion in response to glucose. In mice lacking GLUT2, glucose-induced insulin but not incretin secretion was impaired. Western blot analysis revealed unchanged protein levels of SGLT1 after glucose gavage. GLUT2 detected in apical membrane fractions mainly resulted from contamination with basolateral membranes but did not change in density after glucose administration. SGLT1 is unequivocally the prime intestinal glucose transporter even at high luminal glucose concentrations. Moreover, SGLT1 mediates glucose-induced incretin secretion. Our studies do not provide evidence for GLUT2 playing any role in either apical glucose influx or incretin secretion.

Special report on the Dunkerton Community School District for the period July 1, 2005 through December 31, 2008

Special report on the Dunkerton Community School District for the period July 1, 2005 through December 31, 2008

Iowa School District Surtax Report 2007

Iowa School District Surtax Report 2007