Development of nucleic acid vaccines: use of self-amplifying RNA in lipid nanoparticles

Self-amplifying RNA or RNA replicon is a form of nucleic acid-based vaccine derived from either positive-strand or negative-strand RNA viruses. The gene sequences encoding structural proteins in these RNA viruses are replaced by mRNA encoding antigens of interest as well as by RNA polymerase for replication and transcription. This kind of vaccine has been successfully assayed with many different antigens as vaccines candidates, and has been shown to be potent in several animal species, including mice, nonhuman primates, and humans. A key challenge to realizing the broad potential of self-amplifying vaccines is the need for safe and effective delivery methods. Ideally, an RNA nanocarrier should provide protection from blood nucleases and extended blood circulation, which ultimately would increase the possibility of reaching the target tissue. The delivery system must then be internalized by the target cell and, upon receptor-mediated endocytosis, must be able to escape from the endosomal compartment into the cell cytoplasm, where the RNA machinery is located, while avoiding degradation by lysosomal enzymes. Further, delivery systems for systemic administration ought to be well tolerated upon administration. They should be safe, enabling the multiadministration treatment modalities required for improved clinical outcomes and, from a developmental point of view, production of large batches with reproducible specifications is also desirable. In this review, the concept of self-amplifying RNA vaccines and the most promising lipid-based delivery systems are discussed.

Neurolinguistic measure of typological effects in multilingual transfer: introducing an ERP methodology

This article has two main objectives. First, we offer an introduction to the subfield of generative third language (L3) acquisition. Concerned primarily with modeling initial stages transfer of morphosyntax, one goal of this program is to show how initial stages L3 data make significant contributions toward a better understanding of how the mind represents language and how (cognitive) economy constrains acquisition processes more generally. Our second objective is to argue for and demonstrate how this subfield will benefit from a neuro/psycholinguistic methodological approach, such as event-related potential experiments, to complement the claims currently made on the basis of exclusively behavioral experiments. Palabras clave

Energy and momentum transfer in one-dimensional trapped gases by stimulated light scattering

In ultracold atoms settings, inelastic light scattering is a preeminent technique to reveal static and dynamic properties at nonzero momentum. In this work, we investigate an array of one-dimensional trapped Bose gases, by measuring both the energy and the momentum imparted to the system via light scattering experiments. The measurements are performed in the weak perturbation regime, where these two quantities-the energy and momentum transferred-are expected to be related to the dynamic structure factor of the system. We discuss this relation, with special attention to the role of in-trap dynamics on the transferred momentum.

Farming experiments and transfer of technology of bivalve culture along the southwest coast of India

The Central Marine Fisheries Research Institute (CMFRI) in India developed bivalve farming technologies in the 1970s, but these were not widely adopted at the time. In 1993, CMFRI undertook an action research program to encourage farming of edible oysters (Crassostrea madrasensis), mussels (Perna viridis and Perna indica), clams (Paphia malabarica) and pearls (Pinctada fucata) along the southwest coast of India. Successful demonstration of the viability of bivalve farming led to the initiation of commercial farming of mussels and generated interest among farmers and entrepreneurs in developing production of pearls and farming of edible oysters. Given the high potential for the mollusc aquaculture, both for the local and export market, issues such as demarcation and issuance of lease right on aquaculture zones in public waterbodies by the government, organization of marketing systems and provisions for technical and financial support to farmers need to be addressed.

Aquaculture technology transfer to smallholder farmers in Malawi, Southern Africa

Details are given of activities conducted in Zomba, Malawi, in order to demonstrate new aquaculture technologies and encourage their use by smallholder fish farmers. The following technologies were introduced: napier grass as a pond output; use of a reed fence for harvesting fish; developing a high-quality compost as a pond input; vegetable-pond integration; chicken-pond integration; smoking kiln; pond stirring; and rice-fish integration. The reactions of the farmers to these technologies and their testing by the farmers are outlined briefly.