Indirect validation of the age-reading method for Pacific cod (Gadus macrocephalus) using otoliths from marked and recaptured fish

Two examples of indirect validation are described for age-reading methods of Pacific cod (Gadus macrocephalus). Aging criteria that exclude faint translucent zones (checks) in counts of annuli and criteria that include faint zones were both tested. Otoliths from marked and recaptured fish were used to back-calculate the length of each fish at the time of its release by using measurements of the area of annuli. Estimated fish size at time of release and actual observed fish size were similar, supporting the assumption that translucent zones are laid down on an annual basis. A second method for validating reading criteria used otolith age and von Bertalanffy parameters, estimated from the tagging data, to predict how much each fish grew in length after tagging. We found that otolith aging criteria applied to otoliths from tagged and recovered Pacific cod predicted quite accurately the growth increments that we observed in these specimens. These results provide further evidence that the current aging criteria are not underestimating the age of the fish and support our current interpretation of checks (i.e., as subannual marks). We expect these indirect validations to advance age determination for Pacific cod, which in turn would enhance development of stock assessment methods based on age structure for this species in the eastern Bering Sea.

Erros pré-analíticos em medicina laboratorial: uma revisão sistemática

MENEZES, Patrick Lourenço. Erros pré-analíticos em medicina laboratorial: uma revisão sistemática. 2013. 98 f. Dissertação (Mestrado em Saúde, Medicina Laboratorial e Tecnologia Forense) - Instituto de Biologia Roberto Alcântara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2013. A relevância evidente dos erros pré-analíticos como problema de saúde pública fica patente tanto no dano potencial aos pacientes quanto nos custos ao sistema de saúde, ambos desnecessários e evitáveis. Alguns estudos apontam que a fase pré-analítica é a mais vulnerável a erros, sendo responsável por, aproximadamente, 60 a 90% dos erros laboratoriais em consequência da falta orientação aos pacientes sobre os procedimentos que serão realizados no laboratório clínico. Objetivos: Sistematizar as evidências científicas relacionadas aos erros pré-analíticos dos exames laboratoriais de análises clínicas. Método: Uma revisão sistemática foi realizada, buscando as bases de dados do Medical Literature Analysis and Retrieval System Online (MEDLINE), Scopus(que inclui MEDLINE e Embase), ISI Web of Knowledge, SciFinder, Literatura Latino-Americana e do Caribe em Ciências da Saúde (Lilacs) (que inclui a Scientific Electronic Library Online SciELO) e o Índice Bibliográfico Espanhol de Ciências de Saúde (IBECS), para artigos publicados entre janeiro de 1990 e junho de 2012 sobre erros de exames laboratoriais que possam ocorrer na fase pré-analítica. Os estudos foram incluídos de acordo com os seguintes exames laboratoriais: hemograma, análise bioquímica do sangue total ou do soro, exames de coagulação sanguínea,uroanálise e exames hematológicos ou bioquímicos em outros materiais e categorizados pelo tipo de erro pré-analítico e pela frequência dos incidentes. Resultados: A busca nas bases de dados bibliográficas resultou no seguinte número de artigos recuperados: 547 na MEDLINE, 229 na Scopus, 110 na ISI, 163 na SciFinder, 228 na Lilacs e 64 na IBECS, perfazendo um total de 1.341 títulos. Ao fim da revisão sistemática, obteve-se um conjunto de 83 artigos para leitura de texto completo, dos quais 14 foram incluídos na revisão. Os estudos abrangeram diferentes tipos de laboratórios, setores técnicos e origem de erros, segundo a fase do processo laboratorial. Discussão: Sete artigos demonstraram erros de pedidos médicos, com uma alta variabilidade nos valores de incidência. Os seis artigos que estudaram erros de coleta de amostra observaram redução deste desfecho. As proporções de eventos adversos relatados e os impactos clínicos variaram, levando a consequências descritas como: erros decorrentes da flebotomia, recoleta de amostras, repetições de exames, atrasos na liberação de resultados de exames e possíveis danos ao paciente. Conclusões: O laboratório deve ter instruções por escrito para cada teste, que descreva o tipo de amostra e procedimento de coleta de amostra. Meios de identificação por código de barras, sistemas robóticos e analíticos reduzem os erros pré-analíticos. A melhoria da fase pré-analítica de testes laboratoriais permanece um desafio para muitos laboratórios clínicos.

River Dart Scale Reading Investigation Report on the 1965 Season

This is the fourth River Dart Scale Reading Investigation Report on the 1965 season by the Devon River Board. The object of this investigation is to examine, by means of scale-reading, the general biology of the salmon population of the River Dart. It reviews the methods used for the collection of scales and examination of the materials. It shows the results of the survey and the number of scales studied from each of the various sea-age classes, time of running with distribution of the sea-age groups throughout the season, fish sizes and smolt ages at migration. All it summarized in tables, and figures are included plotting weight distributions for each age classes and frequency distributions.

River Dart Scale Reading Investigation Report on the 1966 Season

This is the fifth River Dart Scale Reading Investigation Report on the 1966 Season by the Devon River Board. The object of this investigation is to examine, by means of scale-reading, the general biology of the salmon population of the River Dart. It reviews the methods used for the collection of scales and examination of the materials. It shows the results of the survey and the number of scales studied from each of the various sea-age classes, time of running with distribution of the sea-age groups throughout the season, fish sizes and smolt ages at migration. All it summarized in tables, and figures are included plotting weight distributions for each age classes and frequency distributions. It also compares the results of previous reports.

River Dart Scale Reading Investigation Report on the 1967 Season

This is the sixth and final River Dart Scale Reading Investigation Report on the 1967 Season by the Devon River Board. The object of this investigation is to examine, by means of scale-reading, the general biology of the salmon population of the River Dart. It reviews the methods used for the collection of scales and examination of the materials. It shows the results of the survey and the number of scales studied from each of the various sea-age classes, time of running with distribution of the sea-age groups throughout the season, fish sizes and smolt ages at migration. All it summarized in tables, and figures are included plotting weight distributions for each age classes and frequency distributions. It also compares the results of previous reports and gives a full summary for the investigation (including previous reports).

River Dart Scale Reading Investigation Report on the 1962 Season

This is the first River Dart Scale Reading Investigation Report on the 1962 season by the Devon River Board. The object of this investigation is to examine, by means of scale-reading, the general biology of the salmon population of the River Dart. It reviews briefly the theory and mechanics of the scale-reading technique, the life history of the salmon, and the methods used for the collection of scales and the examination of the materials. It shows the results of the survey and the number of scales studied from each of the various sea-age classes, time of running with distribution of the sea-age groups throughout the season, fish sizes and smolt ages at migration. All are summarized in various tables and figures are also included, plotting weight distributions for each age classes and frequency distributions.

River Dart Scale Reading Investigation Report on the 1963 Season

This is the second River Dart Scale Reading Investigation Report on the 1963 season by the Devon River Board. The object of this investigation is to examine, by means of scale-reading, the general biology of the salmon population of the River Dart. It reviews the methods used for the collection of scales and examination of the materials. It shows the results of the survey and number of scales studied from each of the various sea-age classes, time of running with distribution of the sea-age groups throughout the season, fish sizes and smolt ages at migration. All are summarized in tables, and figures are alo included plotting weight distributions for each age classes and frequency distributions. The report also remarks the similarity of the results with those for 1962.

River Dart Scale Reading Investigation Report on the 1964 Season

This is the third River Dart Scale Reading Investigation Report on the 1964 season by the Devon River Board. The object of this investigation is to examine, by means of scale-reading, the general biology of the salmon population of the River Dart. It reviews the methods used for the collection of scales and examination of the materials. It shows the results of the survey and the number of scales studied from each of the various sea-age classes, time of running with distribution of the sea-age groups throughout the season, fish sizes and smolt ages at migration. All are summarized in tables, and figures are also included plotting weight distributions for each age classes and frequency distributions. After three years of the investigation a pattern is emerging which shows that the Dart salmon population is mainly composed of four year old fish, which have spent two years in the river and then two year or little longer at sea.

River Dart Scale Reading Investigation 1971

This is the River Dart Scale Reading Investigation Report on the 1971 season by the Devon River Board. The object of this investigation is to examine, by means of scale-reading, the general biology of the salmon population of the River Dart. In 1967 the final report on a five-year scale reading programme was summarised, at the time it was considered sufficient information to help with restocking programmes and the management of salmon population. But a further scale reading programme was started in an attempt to monitor changing patterns in salmon runs that may be caused by the new influences. It reviews the methods used for the collection of scales and examination of the materials. It shows the results of the survey and the number of scales studied from each of the various sea-age classes, time of running with distribution of the sea-age groups throughout the season, fish sizes and smolt ages at migration. All are summarized in tables, and figures are also included plotting weight distributions for each age classes and frequency distributions.