Similarity between the in vitro activity and toxicity of two different fungizone™ / lipofundin™ admixtures

Amphotericin B (AmB), an antifungal agent that presents a broad spectrum of activity, remains the gold standard in the antifungal therapy. However, sometimes the high level of toxicity forbids its clinical use. The aim of this work was to evaluate and compare the efficacy and toxicity in vitro of Fungizon™ (AmB-D) and two new different AmB formulations. Methods: three products were studied: Fungizon™, and two Fungizon™ /Lipofundin™ admixtures, which were diluted through two methods: in the first one, Fungizon™ was previously diluted with water for injection and then, in Lipofundin™ (AmB-DAL); the second method consisted of a primary dilution of AmB-D as a powder in the referred emulsion (AmB-DL). For the in vitro assay, two cell models were used: Red Blood Cells (RBC) from human donors and Candida tropicallis (Ct). The in vitro evaluation (K+ leakage, hemoglobin leakage and cell survival rate-CSR) was performed at four AmB concentrations (from 50 to 0.05mg.L-1). Results: The results showed that the action of AmB was not only concentration dependent, but also cellular type and vehicle kind dependent. At AmB concentrations of 50 mg.L-1, although the hemoglobin leakage for AmB-D was almost complete (99.51), for AmB-DAL and AmB-DL this value tended to zero. The p = 0.000 showed that AmB-D was significantly more hemolytic. Conclusion: The Fungizon™- Lipofundin™ admixtures seem to be the more valuable AmB carrier systems due to their best therapeutic index presented

Soroepidemiologia da infecção pelo Trypanosoma cruzi na zona rural do semiárido do estado do Rio Grande do Norte, Brasil

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Estudo do efeito dos metabólitos do benzeno e tolueno sobre as mitocôndris cerebrais e hepáticas de ratos

Aim: The aim of this work was to investigate the hypothesis that catechol and 3MC inhibit FADH2-linked basal respiration in mitochondria isolated from rat liver and brain homogenates. Moreover, catechol ability to induce DNA damage in rat brain cells through the comet assay (alkaline single-cell gel electrophoresis assay) was also observed. Methods: Two different catechols were evaluated: pirocatechol (derived from benzene) and 3-methylcatechol (derived from toluene); rat liver and brain homogenates were incubated with 1mM catechol at pH 7.4 for up to 30 minutes. After that, mitochondrial fractions were isolated by differential centrifugation. Basal oxygen uptake was measured using a Clark-type electrode after the addition of 10 mM sodium succinate for a period of 12 minutes. In additional experiments, rat brain cells were treated with 1, 5 and 10mM pirocatechol for up to 20 minutes at 37º C, and submitted to electrophoresis. Results: Catechols (pirocatechol and 3methylcatechol) induced a time-dependent partial inhibition of FADH2-linked basal mitochondrial respiration. Indeed, pirocatechol was able to produce a dosedependent DNA oxidative damage in rat brain cells by 2 and 4 injury levels. These results suggest that reactive oxygen species generated by the oxidation of catechols, induced an impairment on mitochondrial respiration and a DNA damage, which might be related to their citotoxicity. Conclusion: Catechols produced an inhibition of basal respiration associated to FADH2 in isolated liver and brain mitochondria; 3-methylcatechol, at the same concentration, produced similar toxicity in the mitochondrial model. Indeed, pirocatechol induced a DNA damage in rat brain cells, mainly observed in comets formation and consequent DNA degradation

Análise das interpretações do governo Evo Morales: o legado histórico e o poder nacional-indígena

This work aims to analyze the interpretations about Evo Morales' government in Bolivia. For such, it proposes a theoretical reclaim of Marxism in Latin America, as well as of Bolivian political history since the 1952 Revolution, going through the crisis in Pacted Democracy intensified in the five-year conjuncture of struggles started in 2000 up to the election and reelection of Morales. It departs from an empirical prior conjecture taken from a qualitative analysis and a broad literature review to analyze the different interpretations of the Bolivian political process from Marxist theoretical matrices. After this historical recovery and this reading of contemporary Bolivia, it concludes with a consideration about the formation of a possible new block of power in the country, with the retaking of nationalism and Indianism as revolutionary reasons

O Trabalho e o labirinto da responsabilidade socioempresarial:a experiência da cidadania empresarial do Banco do Brasil

In the current systemic crisis, economic policy is directed to correct the consequences of the functioning of this metabolism, but within the limits of the capital. From this perspective, decision makers propose trade policies, agricultural and industrial to ensure conditions for economic growth. However, as a dead end, there is failure of the State in giving efficacy to the operation of all segments of the economy, especially given the budget constraint. Public managers are forced to seek external resources, resuming the cycle of political allegiance to the interests of international financial and banking representatives, installed in so-called multilateral. The complex ideological capital comes into play in trying to convince society that the paths taken by governments are inevitable, and that capitalism can be "humanized", even with the realization of the growing inequalities caused by historical irrationalism of the production process of capital . In this sense, emerging concepts that attempt to demonstrate the compatibility of the system to real human needs. This ideological offensive is intended to legitimize the capital. The so-called third sector has a special highlight with the concept of corporate social responsibility. It creates a political environment in which the inevitable mix-up with new illusions offered by and often funding the metabolism of capital in order to perpetuate this system. In this context, political elites, and considerable portions of the academy, embark on "waves of capitalist optimism," while the sociometabolismo capital expands its historical limits, driving forces postponing their collapse, but that cause human suffering and ecological stress. Wars are disseminated to strengthen the deadly war industry and the automobile industry; and devastating the environment of which depends the capital system. In this scenario disassemble, propositions emerge around a "new social pact" in order to minimize the adverse effects of the dynamics of reproduction of capital. The business class is called to exercise its role through the discourse that appeals to social responsibility programs, in order to intervene directly in the "social question". The core of this research is precisely this point. Although there is considerable scholarship on the phenomenon of Social Responsibility and Corporate Citizenship, there is also an evident lack of this approach focused on the banking sector in Brazil. The importance of rentier capital increased ownership of shares in the wealth produced by all of Brazilian society, justifies a sociological research project on Social Responsibility in the domestic financial sector. In this sense, it was decided to perform a dynamic approach to the "Corporate Citizenship" in the banking industry, specifically in the Bank of Brazil. As this is a key institution, is important analyze of the impacts of this strategy fetish of capitalist reproduction, in order to evaluate the social legitimization of rentier capital in Brazil. In this scenario of the abundance of the discourse on social responsibility there exist a progressive impoverishment of professional work in this segment in Brazil. There is a dramatic mismatch between rhetoric and practice because of the trend of deepening vulnerability of the working conditions of the Brazilian bank worker, from the 1990's. In the specific case of the Bank of Brazil, the first initiative of the institution was to conform to the principles of the UNO and the Ethos Institute, aiming to align their domestic policies to this new strategy of domination of capital. The purpose is to place the Bank in the ideological sphere of corporate social responsibility, just as with its partners in the private financial intercapitalist competition. Indeed, in the internal ambit of the Bank of Brazil, there is a policy to adjust its functional segments to the doctrine of Social Corporate Responsibility. The concepts of this doctrine is presented as something inexorable. There are no alternatives. The Bank of Brazil operates in a highly competitive market, the segment featuring the dominance of financial capital accumulation today. For this reason it can not fail to incorporate the technological advances organizational. For employees there is no alternative but to adapt to this new set of ideas proposed by the metabolism of capital

Influência do folículo dominante sobre a dinâmica folicular ovariana em vacas Nelore tratadas com FSH

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desempenho comparativo entre duas linhagens de roedeiras comerciais debicadas em diferentes idades na fase de recria

O experimento foi realizado com o objetivo de comparar o desempenho produtivo de duas linhagens comerciais de poedeiras debicadas em diferentes idades na fase de recria. Foram utilizadas 250 aves da linhagem Lohmann (LSL) e 250 aves da linhagem Isa Babcock (IB), debicadas em diferentes idades. A primeira debicagem foi realizada no 8º dia de idade e a segunda na 8ª, 10ª, 12ª ou 14ª semana de idade. Um grupo de 32 aves permaneceu com o bico intacto e outro não foi submetido à segunda debicagem. O delineamento utilizado foi em blocos ao acaso, em esquema fatorial 2x6 (linhagens x idade de debicagem). Não foi verificado efeito significativo da idade de debicagem sobre os parâmetros produtivos analisados, exceto a melhoria significativa na produção de ovos. As aves da linhagem LSL apresentaram melhores resultados para maturidade sexual, peso médio dos ovos, produção de ovos/ave dia, porcentagem de ovos quebrados e conversão alimentar, quando comparadas às aves da linhagem IB. Embora a prática da debicagem tenha melhorado a porcentagem de postura, os resultados evidenciaram que os parâmetros produtivos analisados foram mais influenciados pela linhagem do que pela idade de debicagem.

A busca de sintomáticos respiratórios de tuberculose na prática e perspectiva do agente comunitário de saúde nos distritos sanitários de Natal

The objective of this work - which is characterized analyze the search for symptomatic tuberculosis in practice and perspective of the Community Health Agent (ACS) in the districts of Natal. Methods: This is a cross-sectional study. The study population was 646 professionals, and conducted a probabilistic random sampling, stratified by districts. The data were collected from one instrument to collect data based on Primary Care Assesment Toll (PCAT) and analyzed by descriptive statistics. The sample consisted of ACS was 87% female. Among the study participants 58% completed high school and 120 months of exercise training (95% CI 111.9 to 129.5) on average. 90% were USF. The average follow-up of cases found were 2 cases of TB since the beginning of the career of the ACS and the last three years the average is presented in a case accompanied. The ACS received satisfactory ratings on the bond of trust with the user, so as access to homes in the community. The ACS reported for denying the fear of being positive result was the biggest reason for not performing the sputum. All units have a professional that responds to the Tuberculosis Control Program. Regarding the structural capacity of primary care settings for the diagnosis of TB, we observed satisfactory levels in different districts of pots for sputum collection, however, a point that deserves attention from managers is lack of materials for packaging sputum. Fear of positive result was one of the reasons for the refusal of sputum collection, followed by alcoholism. With regard to TB suspects, all responded that ACS always suspect when the user has TB coughs, but in all districts were noticed at low delivery of requests for applications for smear. BSR in TB control, is characterized in practice as a complex action goes beyond technical expertise and contact with the family that breaks with the Cartesian. The BSR is part of the ACS can perform them from the daily visits. We conclude that the ACS is difficult to achieve. This practice should not be the privilege of this actor, but the entire team of primary care. We must rethink the practices of TB care, seeing the health surveillance while aegis of the working process of primary care teams for early diagnosis and thereby reduce TB in communities

Injúria pelo frio na qualidade pós-colheita de mangas cv. Palmer

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Inclusão da camomila no desempenho, comportamento e estresse em codornas durante a fase de recria

Este estudo teve como objetivo avaliar o efeito da Matricaria chamomila sobre o estresse de codornas japonesas na fase de recria (28 a 42 dias de idade). Foram utilizadas 192 codornas com 28 dias de idades, distribuídas em blocos casualizados e submetidas às dietas com 0, 250, 500 e 750mg de camomila/kg de ração, totalizando quatro tratamentos, com oito repetições e seis aves por parcela. Foram avaliados os parâmetros de desempenho (consumo diário de ração, conversão alimentar e ganho de peso), comportamentais (tempo em imobilidade tônica, ferimentos corporais e agressividade) e fisiológicos (concentração plasmática de corticosterona e relação heterófilo:linfócito). Os resultados obtidos demonstraram que a camomila adicionada na dieta não foi capaz de alterar os parâmetros de desempenho, bem como os de comportamento e fisiológicos.

Passionflower supplementation in diets of Japanese qualis at rearing and laying periods

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de um equipamento para avaliação do desempenho de geomantas

O controle sobre os processos erosivos é uma necessidade constatada em vários segmentos da engenharia. A movimentação de terra necessária para a execução de taludes rodoviários, por exemplo, pode resultar em taludes de corte ou aterro vulneráveis à erosão superficial. Dentre as alternativas tecnológicas para controle de erosão a utilização de geossintéticos se apresenta como uma solução potencial. A referida aplicação encontra-se em amplo desenvolvimento em alguns países como, por exemplo, nos EUA. No Brasil, a especificação de geossintéticos para controle de erosão é limitada pela ausência de caracterização desses produtos e de normas nacionais, sendo a única fonte técnica de informação, os catálogos dos fabricantes. Neste contexto, o objetivo deste trabalho é construir um equipamento e desenvolver métodos de ensaio para caracterização e avaliação de geossintéticos utilizados no controle de erosão superficial, com base na ASTM D7101. Além de um simulador de chuvas, o equipamento é composto por uma bancada de testes formada por: rampa de escoamento, mesa de suporte e núcleos de solo. Utilizando a bancada construída, foram realizados ensaios para avaliar o funcionamento do equipamento e o desempenho de uma geomanta na redução da taxa de erosão superficial. Os ensaios foram realizados com intensidades de precipitação de 100 ± 4mm/h e 150 ± 4mm/h, durante 30 minutos, com intervalo de leitura de 5 minutos. Os resultados obtidos nos ensaios sem a presença da geomanta mostraram uma perda de solo acentuada durante as chuvas simuladas, com uma iv tendência de crescimento linear da perda de solo acumulada em função do tempo de ensaio. Nos ensaios realizados com a presença da geomanta observou-se a ação de proteção do geossintético com uma redução da ordem de 90% da perda de solo acumulada para todas as intensidades de chuvas utilizadas

Triptofano na alimentação de codornas japonesas nas fases de recria e postura

Objetivou-se estudar o efeito de diferentes planos alimentares, de acordo com os níveis de triptofano, sobre o desempenho e estresse (pela avaliação de parâmetros fisiológicos) de codornas japonesas (Coturnix coturnix japonica) nas fases de recria e postura. Cento e noventa e duas codornas japonesas na fase de recria (30 a 44 dias de idade) foram alojadas em gaiolas e distribuídas em um delineamento em blocos casualizados, com quatro planos de nutrição [0,27 (controle); 0,52; 0,77 ou 1,02% de triptofano], seis repetições e oito aves por parcela. Foram avaliados os parâmetros de desempenho (consumo de ração, ganho de médio peso, conversão alimentar e taxa de mortalidade) e os parâmetros fisiológicos das aves (relação heterófilo/linfócito e concentração de corticosterona no plasma sangüíneo). Outras 192 codornas japonesas na fase de postura (45 a 146 dias) foram distribuídas em delineamento de blocos ao acaso e submetidas a quatro planos de nutrição (0,23 [controle]; 0,48; 0,73 ou 0,98% de triptofano), com seis repetições e oito aves por parcela. Foram avaliados o consumo de ração, a produção de ovos, a conversão alimentar por dúzia de ovos, a taxa de mortalidade, a relação heterófilo/linfócito e a concentração de corticosterona no plasma. Os níveis de triptofano testados não afetaram o desempenho e os parâmetros fisiológicos de codornas japonesas nas fases de recria e postura.

Occurrence of Physoclypeus farinosus Hendel (Diptera : Lauxaniidae) in flowerheads of Asteraceae (Asterales)

The occurrence of Physoclypeus farinosus Hendel in flowerheads of Asteraceae from different Brazilian localities is presented. The use of this resource by this fly is discussed.