Mouaille Dans Le Sahe Lane ; K'sida Tal Guandouze : sika / Thatha El Ralpya, chant [acc. instr.]

[Traditions. Afrique du Nord. Maroc]

Evidence for different expression profiles for c-Met, EGFR, PTEN and the mTOR pathway in low and high grade endometrial carcinomas in a cohort of consecutive women. Occurrence of PIK3CA and K-Ras mutations and microsatellite instability.

Molecular and genetic investigations in endometrial carcinogenesis may have prognostic and therapeutic implications. We studied the expression of EGFR, c-Met, PTEN and the mTOR signalling pathway (phospho-AKT/phospho-mTOR/phospho-RPS6) in 69 consecutive tumours and 16 tissue microarrays. We also analysed PIK3CA, K-Ras mutations and microsatellite instability (MSI). We distinguished two groups: group 1 (grade 1 and 2 endometrioid cancers) and group 2 (grade 3 endometrioid and type II clear and serous cell cancers). We hypothesised that these histological groups might have different features. We found that a) survival was higher in group 1 with less aggressive tumours (P⟨0.03); b) EGFR (P=0.01), PTEN and the AKT/mTOR/RPS6 signalling pathway were increased in group 1 versus group 2 (P=0.05 for phospho-mTOR); c) conversely, c-Met was higher (P⟨0.03) in group 2 than in group 1; d) In group 1, EGFR was correlated with c-Met, phospho-mTOR, phospho-RPS6 and the global activity of the phospho-AKT/phospho-mTOR/phospho-RPS6 pathway. In group 2, EGFR was correlated only with the phospho-AKT/phospho-mTOR/phospho-RPS6 pathway, whereas c-Met was correlated with PTEN; e) survival was higher for tumours with more than 50% PTEN-positive cells; f) K-RAS and PIK3CA mutations occurred in 10-12% of the available tumours and MSI in 40.4%, with a loss of MLH1 and PMS2 expression. Our results for endometrial cancers provide the first evidence for a difference in status between groups 1 and 2. The patients may benefit from different targeted treatments, anti-EGFR agents and rapamycin derivatives (anti-mTOR) for group 1 and an anti c-MET/ligand complex for group 2.

Free-breathing 3D steady-state free precession coronary MR angiography with radial k-space sampling: comparison with cartesian k-space sampling and cartesian gradient-echo coronary MR angiography--pilot study.

The authors compared radial steady-state free precession (SSFP) coronary magnetic resonance (MR) angiography, cartesian k-space sampling SSFP coronary MR angiography, and gradient-echo coronary MR angiography in 16 healthy adults and four pilot study patients. Standard gradient-echo MR imaging with a T2 preparatory pulse and cartesian k-space sampling was the reference technique. Image quality was compared by using subjective motion artifact level and objective contrast-to-noise ratio and vessel sharpness. Radial SSFP, compared with cartesian SSFP and gradient-echo MR angiography, resulted in reduced motion artifacts and superior vessel sharpness. Cartesian SSFP resulted in increased motion artifacts (P <.05). Contrast-to-noise ratio with radial SSFP was lower than that with cartesian SSFP and similar to that with the reference technique. Radial SSFP coronary MR angiography appears preferable because of improved definition of vessel borders.

Response of P, K, Mg and NO�������-N contents of carrots to irrigation, soil compaction, and nitrogen fertilisation

Selostus: Maan tiiviyden, sadetuksen ja typpilannoituksen vaikutus porkkanan kivenn��isainepitoisuuteen ja ravinteiden ottoon sek�� nitraatin kertymiseen

Role of the V-ATPase in regulation of the vacuolar fission-fusion equilibrium.

Like numerous other eukaryotic organelles, the vacuole of the yeast Saccharomyces cerevisiae undergoes coordinated cycles of membrane fission and fusion in the course of the cell cycle and in adaptation to environmental conditions. Organelle fission and fusion processes must be balanced to ensure organelle integrity. Coordination of vacuole fission and fusion depends on the interactions of vacuolar SNARE proteins and the dynamin-like GTPase Vps1p. Here, we identify a novel factor that impinges on the fusion-fission equilibrium: the vacuolar H(+)-ATPase (V-ATPase) performs two distinct roles in vacuole fission and fusion. Fusion requires the physical presence of the membrane sector of the vacuolar H(+)-ATPase sector, but not its pump activity. Vacuole fission, in contrast, depends on proton translocation by the V-ATPase. Eliminating proton pumping by the V-ATPase either pharmacologically or by conditional or constitutive V-ATPase mutations blocked salt-induced vacuole fragmentation in vivo. In living cells, fission defects are epistatic to fusion defects. Therefore, mutants lacking the V-ATPase display large single vacuoles instead of multiple smaller vacuoles, the phenotype that is generally seen in mutants having defects only in vacuolar fusion. Its dual involvement in vacuole fission and fusion suggests the V-ATPase as a potential regulator of vacuolar morphology and membrane dynamics.

Predacion de semillas de Amaranthus quitensis H.B.K. en un cultivo de soja: influencia del sistema de siembra

El objetivo fue evaluar las p��rdidas del banco superficial de semillas de Amaranthus quitensis H.B.K. (yuyo colorado) debidas a la predaci��n por insectos en un cultivo de soja y en el barbecho posterior, en dos sistemas de laboreo. Los experimentos se realizaron durante las campa��as 94/95 y 95/96. Para calcular la tasa de predaci��n se emplearon bandejas cubiertas con tejido para evitar el ingreso de roedores y con tela de tul en los tratamientos testigos; en cada una se sembraron 100 semillas de la maleza y cada 15 d��as se registr�� el n��mero de semillas remanentes. Para determinar los insectos presentes y su abundancia se emplearon trampas "pitfall". Entre los insectos capturados se encontr�� el car��bido Notiobia cupripennis, su mayor abundancia se registr�� en marzo (4,5 y 5,8 insectos/trampa en convencional y 2,7 y 3,3 insectos/trampa en siembra directa), coincidiendo con las tasas de predaci��n m��s altas (5,6% y 8% en convencional y 2,7% y 3,8% en siembra directa); tanto en abundancia como en predaci��n se observaron diferencias significativas entre ambos sistemas. A partir de este mes, las diferencias no fueron significativas, el n��mero de insectos y la tasa de predaci��n disminuyeron. En ambos a��os existi�� una correlaci��n positiva entre estas variables.

MRI of coronary vessel walls using radial k-space sampling and steady-state free precession imaging.

OBJECTIVE: The objective of our study was to investigate the impact of radial k-space sampling and steady-state free precession (SSFP) imaging on image quality in MRI of coronary vessel walls. SUBJECTS AND METHODS: Eleven subjects were examined on a 1.5-T MR system using three high-resolution navigator-gated and cardiac-triggered 3D black blood sequences (cartesian gradient-echo [GRE], radial GRE, and radial SSFP) with identical spatial resolution (0.9 x 0.9 x 2.4 mm3). The signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), vessel wall sharpness, and motion artifacts were analyzed. RESULTS: The mean SNR and CNR of the coronary vessel wall were improved using radial imaging and were best using radial k-space sampling combined with SSFP imaging. Vessel border definition was similar for all three sequences. Radial k-space sampling was found to be less sensitive to motion. Consistently good image quality was seen with the radial GRE sequence. CONCLUSION: Radial k-space sampling in MRI of coronary vessel walls resulted in fewer motion artifacts and improved SNR and CNR. The use of SSFP imaging, however, did not result in improved coronary vessel wall visualization.

Probabilidade de resposta da cana-de-a����car �� aduba����o pot��ssica em raz��o da rela����o K+ (Ca2++Mg2+ )-0,5 do solo

O objetivo deste trabalho foi avaliar a probabilidade de resposta da produ����o de cana-de-a����car �� aduba����o pot��ssica, em raz��o da rela����o K+ (Ca2++Mg2+ )-0,5 no solo. Foram compilados dados de 106 experimentos de aduba����o pot��ssica na cana-de-a����car. Em cada experimento foi registrado o ciclo de cultivo (cana-planta ou cana-soca), os teores de K, Ca e Mg do solo antes da aduba����o pot��ssica, a rela����o K+ (Ca2++Mg2+ )-0,5, e se houve, ou n��o, resposta estatisticamente significativa da produ����o �� aduba����o pot��ssica. Foi utilizado o m��todo estat��stico de regress��o log��stica, efetuado pelo procedimento CATMOD do Statistical Analysis System. A caracter��stica ciclo de cultivo foi eliminada do modelo, pois esta se apresentou como n��o-significativa no ajuste estat��stico. A rela����o K+ (Ca2++Mg2+ )-0,5 do solo influenciou a probabilidade de resposta da produ����o de cana-de-a����car �� aduba����o pot��ssica. �� medida que a rela����o K+ (Ca2++Mg2+ )-0,5 aumentou, a probabilidade de resposta da produ����o de cana-de-a����car �� aduba����o pot��ssica diminuiu. A rela����o K+ (Ca2++Mg2+ )-0,5 no solo foi classificada em baixa (<0,2547), m��dia (0,2547 a 0,3349) e alta (>0,3349). A rela����o K+ (Ca2++Mg2+ )-0,5 no solo deve ser usada como mais um crit��rio para orientar a aduba����o pot��ssica na cultura da cana-de-a����car.