Avaliação do potencial antagônico de leveduras, visando biocontrole de deterioração por penicillium expansum

Considerable losses during apple fruit storage occur due to microbiological diseases, mainly caused by Penicillium expansum, which in addition to fruit pulp deterioration produces patulin, a mycotoxin with carcinogenic and teratogenic activity. Biological control of post-harvest disease by antagonist yeasts focused on killer toxins is an appreciable alternative to the chemical fungicides, due to the low possibility of toxic residues demonstrated during fermentative processes. Twenty out of 44 yeasts (16 isolated from fruits, 10 from corn silage and 18 from laboratory anthill), showed antagonism against spores of P. expansum. The assay in solid medium pointed the strongest nutrient competition antagonism by D. hansenii strain C1 (31 mm inhibition diameter), while D. hansenii strain C7 (15 mm) showed higher antibiosis and parasitism pattern. In the following step the extracellular activity was tested performing the assay with culture supernatant in Yeast Medium agar, where C. guilliermondii P3 was more effective against conidia germination (inhibition rate of 58.15%) while P. ohmeri showed better inhibition on micelial growth (66.17%). The antibiosis showed by both yeasts could suggest probable mechanism associated with killer phenomenon, once both strains were killer positive against sensitive reference strains (S. cerevisiae NCYC 1006 and P. kluyveri CAY-15). In order to enhance the production of antifungal substance, these yeasts were cultivated with P. expansum, but the difference between culture supernatant obtained from yeasts cultivated alone and with mould was not significant (P > 0.05). The results demonstrated that the yeasts application constitute a promising tool, enhancing the biological control of P. expansum in post-harvest diseases of apple fruit.

Histologia e histoquímica do intestino anterior de tilápia do Nilo (Oreochromis niloticus) alimentadas com dietas contendo silagem de peixe

This work was carried out to evaluate the action of the fermented fish silage and fish meal in the histochemical of the middle intestine of O. niloticus. A great epithelium intestinal was present in fishes fed with fish silage, independent of the animal origin protein proportions in diets. It was observed that the variation of the goblet cells secretion glicoproteic intensity is linked directly to the diet supplied to animals. The middle intestine of Oreochromis niloticus showed differences in the neutral mucus glicoproteic, acid glicoproteic and glicoconjugated secretion pattern, depending on the protein origin and the percentage used in the diet, demonstrating that this species can adapt its secretion system for the protection of the digesting apparatus during the absorption of different protein sources.

Rumen microbial diversity under influence of a polyclonal antibody preparation against lactate-producing and proteolytic bacteria in cows fed different energy sources

Nine ruminally cannulated cows fed different energy sources were used to evaluate an avianderived polyclonal antibody preparation against specific ruminal bacteria and monensin on microbial community diversity. The experimental design was three Latin squares 3 x 3 distinguished by the main energy source in the diet [dry-ground corn grain, high moisture corn silage or citrus pulp]. Inside each Latin square, animals received one of the feed additives per period [control, monensin or polyclonal antibody preparation]. Each period lasted 21 days where 20 were used for treatments adaptation and the last one for sampling collection. Microbial diversity was evaluated by protozoa counts and denaturing gradient gel electrophoresis. Polyclonal antibodies plus citrus pulp (CiPu) addition in the diet resulted in an increase of relative counting of Isotricha protozoa that indicates a possible effect on this ruminal ciliate population. In general lines, in the present experiment, it was not possible to assign that there was a pattern in the structures of amplification of Bacteria and Archaea communities of the ruminal content. Oral passive immunization is a technology that arises as an effective alternative for feed additive production. Further research is still necessary to better understand its mechanisms of action.

Degradabilidade ruminal da matéria seca de grãos de milho e de sorgo com alto ou baixo conteúdo de tanino processados

This work evaluated the effects of high moisture silage, extrusion and corn and sorghum drying with and without tannin, on the chemical composition and ruminal digestion of dry matter. The experimental design was completely randomized in 3x3 factorial scheme (kinds of grains: processing), for the chemical composition and degradability and three blocks (animals) through in situ method. It was verified significant difference between kinds of grains and processing in relation to the chemical composition, except for crude protein and mineral matter. The contents of etherextract in the three types of grains, were reduced significantly with the high moisture silage, extrusion and drying in relation to the humid material. For the contents of phenols, total and condensed tannin there was effect of hybrid, processing and interaction, and the most elevated contents were found in sorghum with tannin. Although grain composition has been similar, the interaction in ruminal ambient associated to the tannin, promoted differences in the dry matter degradability. It was concluded that sorghum without tannin may be ensiled or extruded, while the sorghum with tannin, must be extruded to improve the degradability and availability of nutrients.

Atributos físicos do solo em diferentes condições de cobertura vegetal em área de plantio direto

The use of cover crops in the soil causes changes in soil attributes influencing in a series of hydro-physical processes, which also modify the ability of soil to support the many activities that it is intended. Thus, the objective of this study was to evaluate the effect of cover crops on physical attributes of the soil. For this, an experiment was carried out on a Typic Hapludox, Jaboticabal State, Brazil, using cover crops of millet, sunn hemp, jack bean, lab-lab and black velvet bean in no-tillage and fallow area (spontaneous vegetation). The characteristics evaluated were the bulk density, macroporosity, microporosity, total porosity, aggregate stability, penetration resistance and organic matter. The incorporation of cover crops has proved to be a beneficial practice for the physical attributes of the soil, allowing a greater aggregate stability compared to fallow in the depth of 0-0.05 m. All cover crops presented values of soil penetration resistance below the critical value of 2 MPa.

Plantas de cobertura e adubação nitrogenada na cultura do algodoeiro

The fiber quality, productivity and favorable climate are considered key points for the development of the cotton crop in the Brazilian Cerrado. This study aimed to evaluate the effect of cover crops and N application in presowing on the cotton crop growth and yield. The experimental design was randomized blocks, consisting of a - three cover crops (radish, white oat and black oat) implanted during the winter period, b - four nitrogen levels (0, 30, 60 and 90 kg ha-1 of N) applied to the millet residues and before cotton sowing. In April 2008, evaluations were realized of plant development and also harvesting was performed of experimental plots of cotton plants. The results showed that the radish provides increased length of branches and cotton bolls of cotton crop, without yield increase, and the use of increasing doses of N up to 90 kg ha-1 decreased the amount of carimã per plant, increasing the number of reproductive branches and cotton yield.

Composição química e degradabilidade ruminal de silagens da cana-de-açúcar tratada com aditivos químicos e bacteriano1

The objective of this trial was to evaluate the effect of the addition of chemical and bacterial additive in the ensiling of sugar cane (Saccharum officinarum L.) on chemical composition, pH, kinectic fraction and in situ degradation of nutritions components of silages. Five rumen-cannulated 1/2 Simental + 1/2 Zebu steers were allotted to a completely randomized design. The steers were placed in individual cages and they were fed with diets with 76% forage (%DM). Five silages were evaluated: control - sugar cane, no additives; urea - sugar cane + 0.5% of urea (wet basis); inoculant - sugar cane inoculated with LactoSilo® (390 g/40 t forage); NaOH - sugar cane + 1.0% of sodium hydroxide (wet basis); CaOH - sugar cane + 0.6% of calcium hydroxide (wet basis). The silage additives with sodium hydroxide showed the highest pH values before (11.20) and after (4.87) for silage. No differences were observed among the silages for dry matter (26.85), crude protein (5.25) and acid detergent fiber (57.21). Fractionation of dry matter and organic matter of silages showed similar behavior, with higher values of the soluble fraction (fraction A) for silages with sodium hydroxide (45.86 and 30.95%) and calcium hydroxide (29.47 and 26.13%). The use of sodium hydroxide allowed obtaining higher values for the degradation of cell wall components of silages from cane sugar. The potencial and effective degradability with 3, 5 and 8%/h of passage rate were respectively 88.44, 64.45, 56.73 and 49.83% for NDF and 82.57, 55.51, 46.72 and 38.83% for ADF, indicating that the use of sodium hydroxide as chemical additives can improve the nutritive value of cane sugar silage.

Substituição dos grãos secos de milho pela silagem de grãos úmidos de milho para vacas da raça holandesa em lactação

In order to evaluate the effects of replacement of dried corn (GSM) for silage moisture corn (HMCS) on production and milk composition were used five Holstein cows, primiparous with a mean of 112 days post delivery, confined in Tie Stall, for 70 days. We used five diets according to NRC to 17.5% CP (DM) and 2.4 Mcal / kg DM, the 40:60 ratio of roughage and concentrate containing soybean meal, sugar cane silage and hay and substitution levels of the GSM HMCS the following treatments: a) 0%, 2) 25%, 3) 50%, 4) 75% and 5) 100%. Milk production and dry matter intake (DMI) were recorded daily. The animals were milked daily 6:00 and 18:00 h, and milk samples collected from consecutive milkings of each experimental period of 14 days (four days of collection). The experimental design was a 5x5 Latin square and the data analyzed by the statistical program SAS. Body weight (508 kg), milk (23.6 kg), corrected milk (22.7 kg), DMI (17.13 kg) showed no significant difference, but the intakes of neutral detergent fiber (6.67 kg), and detergent acid (3.39 kg), feed efficiency for the production of milk (milk 1.41 kg / day) urea nitrogen (17.67 mg / dL) differ, thus indicating that HMCS is more efficient than GSM in the diet of dairy cows not alter the production and milk composition.

Glicerina bruta na dieta de bovinos de corte confinados: Efeito sobre o hemograma

The aim of this study was to evaluate the hemogram in cattle fed crude glycerin. 30 Nellore steers, were used for blood sampling, which were done each 28 days. The animals were confined for 103 days and were fed with five diets containing 0, 7.5, 15, 22.5 and 30% crude glycerin based on dry matter, formulated in a forage:concentrate ratio of 30:70. Corn silage was used as forage and the concentrate was composed by corn grain, soybean hulls, sunflower meal, glycerin, limestone, dicalcium phosphate and salt. The experimental design was a randomized block with five treatments. Orthogonal contrasts were used to determine the linear, quadratic, cubic effects and gliceryn treatments × the control one. There was no treatment effect (P>0,05) on blood concentrations of erythrocytes, hematocrit, basophils, eosinophils, band neutrophils and lymphocytes, but were linearly effect on the concentrations of platelets, hemoglobin and monocytes (P=0.009, P=0.001 and P=0.043, respectivelly), and cubic effect on the concentrations of total leukocytes and segmented neutrophils (P=0.008 and P=0.004, respectivelly). Diets for beef cattle, with forage:concentrate ratio of 30:70 added with crude glycerin promote changes in the erytrhogram and leucogram of these animals.

Phosphorus and potassium budget in the soil-plant system in crop rotations under no-till

Soil management and crop rotations can affect P and K budget in soil, decreasing losses, and increasing fertilizer use efficiency. The P and K budget in the soil-plant system at depths up to 60. cm was studied for different soil managements and crop rotations under no-till for three years in Botucatu, São Paulo, Brazil. The investigated crop rotations were: triticale (X Triticosecale) and sunflower (Helianthus annuus) cropped in autumn-winter; pearl millet (Pennisetum glaucum), forage sorghum (Sorghum bicolor), and Sunn hemp (Crotalaria juncea) were grown in the spring, as well as an additional treatment with chiseling followed by a fallow period; and soybean (Glycini max, L., Merril) was cropped in the summer. Each year triticale and sunflower were grown in plots and pearl millet, forage sorghum, Sunn hemp and of chisel/fallow in sub-plots. The triticale/millet rotation led to the largest decrease in available P within the 0-0.60. m layer of the soil profile and the largest K increase within the 0-0.05. m layer. Potassium mobility in the soil profile and the increases in the available K content in the 0.40-0.60. m layer were independent of the management system. Crop rotations with or without chiseling are not effective in preventing soil P losses. There is considerable K leaching below 0.60. m, but chiseling and the use of high K accumulating plants as triticale results in lower K losses. © 2012 Elsevier B.V.

Absorção de nitrogênio pelo algodoeiro herbáceo em dois sistemas de cultivo

The study of nutrient uptake is of fundamental importance to plant nutrition, as well as tell the time (growth stage) being absorbed by the culture, also signals to the levels of fertilizer to be used. Aiming to establish the uptake of nitrogen for cultivation of cotton (Gossypium hirsutum L. r. latifolium Hutch.) led to an experiment in a Alfissol under randomized block design with two treatments, sowing conventional tillage and after tillage on the straw of previous crop (millet), with five replicates. Samples were taken according to the phenology and plants development. The dry matter accumulation in cotton is linear and increasing until the period of large boll, being more intense with the appearance of flowers and boll, or 50 to 100 days after emergence. The nitrogen content is higher in newly emerged plants, having peaks at the beginning of the appearance of squares and flowers, and from the appearance of small boll, the nitrogen content in the plant decreases linearly. The extraction of nitrogen is bigger from the appearance of flowers (55-60 days after emergence).

The no-tillage system and cover crops-Alternatives to increase upland rice yields

Upland rice (Oryza sativa L.) cultivation has been increasing in importance in Asia while water availability for irrigation has been decreasing because of rapid growth in industry and urban centers. Therefore, the development of technologies that increase upland rice yields under aerobic conditions, thereby saving water, would be an effective strategy to avoid a decrease in global rice grain production. The use of the no-tillage system (NTS) and cover crops that maintain soil moisture would prove advantageous in the move toward sustainable agriculture. However, upland rice develops better in plowed soil, and it has been reported that this crop does not perform well under the NTS. Therefore, the aim of this study was to investigate the effect of cover crops on upland rice grain yield and yield components sowed in a NTS. A field experiment was conducted during two growing seasons (2008-2009 and 2009-2010), and treatments consisted of growing rice under five cover crops in a NTS and two control treatments under the conventional tillage system (plowing once and disking twice). Treatments were carried out in a randomized block design with three replications. Our findings are as follows: On average, Brachiaria brizantha (12.32Mgha-1), Brachiaria ruziziensis (11.08Mgha-1) and Panicum maximum (11.62Mgha-1) had outstanding biomass production; however, these grasses provided the worst upland rice yields (2.30, 2.04, and 2.67Mgha-1, respectively) and are not recommended as cover crops before upland rice. Millet and fallow exhibited the fastest straw degradation (half-lives of 52 and 54 days, respectively), and millet exhibited the fastest nitrogen release (N half-life of 28 days). The use of a NTS was promising when millet was used as a cover crop; this allowed the highest upland rice yield (3.94Mgha-1) and did not statistically differ from plowed fallow (3.52Mgha-1). © 2012 Elsevier B.V.

Consumo, digestibilidade aparente de nutrientes e balanços de nitrogênio e hídrico de ovinos alimentados com silagens de cultivares de capim-búfel

The objective of this present experiment was to determine intake and apparent digestibility of dry matter and nutrients, nitrogen (NB) and water balances (WB) of sheep fed with buffel grass silages. The buffel grass cultivars used were Tanzania, Buchuma and Biloela. 21 male, castrated, crossbred Santa Ines x Non defined genotype sheep, with initial body weight of 31.8±3.16kg were used, kept in metabolic cages. The experimental design was completely randomized with seven replicates. No differences were observed in dry matter intake in g/day (919.2) and % of body weight (2.9). In general, digestibility coefficients of dry matter varied from 37.7% to 60.0%. The NB and WB were positive, with higher NB values in sheep fed with Tanzania (5.1g/day) and Biloela (3.9g/day) and higher WB in animals fed with Buchuma (1.38kg/animal/day) and Biloela (1.42kg/animal/day). All cultivars of buffel grass evaluated (Tanzania, Buchuma and Biloela) presented adequate chemical composition, where the silage of buffel grass used corresponded to 60% of the total daily water intake by sheep.

Plantas de cobertura e qualidade química de latossolo Amarelo sob plantio direto no cerrado Maranhense

Aiming to evaluate the effect of cover plants over soil chemical attributes in the region of Balsas - Maranhão, samples of Oxisol under plant cover of millet [Pennisetum americanum (L.) Leeke], brachiaria (Brachiaria ruziziensis) and native Cerrado (mid-sized), were collected in three depths (0-0.10; 0.10-0.20 and 0.20-0.40 m). Plant cover of millet and brachiaria promoted an increase in soil pH and reductions in amounts of Al3+ and in saturation by Al3+, in relation to the area with native Cerrado. Major amounts of residue were verified on soil surface under native cerrado, due to greater input of plant residue and lower decomposition rate. Amounts of nutrients and levels of organic matter were higher in areas under millet and brachiaria cover, mainly in 0-0.10 e 0.10-0.20 m. depths. Brachiaria and millet cover plants were similar in relation to alterations promoted in chemical attributes of soil.

Search for new physics in final states with a lepton and missing transverse energy in pp collisions at the LHC

This Letter describes the search for an enhanced production rate of events with a charged lepton and a neutrino in high-energy pp collisions at the LHC. The analysis uses data collected with the CMS detector, with an integrated luminosity of 5.0 fb-1 at √s=7 TeV, and a further 3.7 fb -1 at √s=8 TeV. No evidence is found for an excess. The results are interpreted in terms of limits on a heavy charged gauge boson (W ′) in the sequential standard model, a split universal extra dimension model, and contact interactions in the helicity-nonconserving model. For the last, values of the binding energy below 10.5 (8.8) TeV in the electron (muon) channel are excluded at a 95% confidence level. Interpreting the ℓν final state in terms of a heavy W′ with standard model couplings, masses below 2.90 TeV are excluded. © 2013 CERN.