The review of HSS in the case of David and Samual Briggs (The Lewis Report) - Report of the Implementation Team June 2004 (PDF 262 KB)

The review of HSS in the case of David and Samual Briggs (The Lewis Report) - Report of the Implementation Team June 2004

Faecal examination and PCR to detect Strongyloides venezuelensis in experimentally infected Lewis rats

More sensitive methodologies are necessary to improve strongyloidiasis diagnosis. This study compared the sensitivities of the McMaster modified technique and polymerase chain reaction (PCR) assays, both performed in faecal samples. Lewis rats were subcutaneously infected with 4,000, 400 or 40 infective third-stage larvae, considered as high, moderate or low infection, respectively. Seven days later, they were euthanized to count adult nematodes recovered from the small intestine. Stool samples were used to count the number of eggs per gram (EPG) of faeces and to detect parasite DNA by PCR performed with a species and a genus primer pair. The sensitivity of these assays depended upon parasite burden and the primer specificity. All assays presented 100% sensitivity at the highest parasite load. In the moderate infection, EPG and PCR with the genus primer maintained 100% specificity, whereas PCR sensitivity with the species primer decreased to 77.7%. In low infection, the sensitivity was 60% for EPG, 0% for PCR with the species primer and 90% for PCR done with the genus primer. Together, these results suggest that PCR with a genus primer can be a very sensitive methodology to detect Strongyloides venezuelensisin faeces of Lewis rats infected with very low parasite burden.

Ocular and systemic bio-distribution of rhodamine-conjugated liposomes loaded with VIP injected into the vitreous of Lewis rats.

PURPOSE: Local delivery of therapeutic molecules encapsulated within liposomes is a promising method to treat ocular inflammation. The purpose of the present study was to define the biodistribution of rhodamine-conjugated liposomes loaded with vasoactive intestinal peptide (VIP), an immunosuppressive neuropeptide, following their intravitreal (IVT) injection in normal rats. METHODS: Healthy seven- to eight-week-old Lewis male rats were injected into the vitreous with empty rhodamine-conjugated liposomes (Rh-Lip) or with VIP-loaded Rh-Lip (VIP-Rh-Lip; 50 mM of lipids with an encapsulation efficiency of 3.0+/-0.4 mmol VIP/mol lipids). Twenty-four h after IVT injection, the eyes, the cervical, mesenteric, and inguinal lymph nodes (LN), and spleen were collected. The phenotype and distribution of cells internalizing Rh-Lip and VIP-Rh-Lip were studied. Determination of VIP expression in ocular tissues and lymphoid organs and interactions with T cells in cervical LN was performed on whole mounted tissues and frozen tissue sections by immunofluorescence and confocal microscopy. RESULTS: In the eye, 24 h following IVT injection, fluorescent liposomes (Rh-Lip and VIP-Rh-Lip) were detected mainly in the posterior segment of the eye (vitreous, inner layer of the retina) and to a lesser extent at the level of the iris root and ciliary body. Liposomes were internalized by activated retinal Müller glial cells, ocular tissue resident macrophages, and rare infiltrating activated macrophages. In addition, fluorescent liposomes were found in the episclera and conjunctiva where free VIP expression was also detected. In lymphoid organs, Rh-Lip and VIP-Rh-Lip were distributed almost exclusively in the cervical lymph nodes (LN) with only a few Rh-Lip-positive cells detected in the spleen and mesenteric LN and none in the inguinal LN. In the cervical LN, Rh-Lip were internalized by resident ED3-positive macrophages adjacent to CD4 and CD8-positive T lymphocytes. Some of these T lymphocytes in close contact with macrophages containing VIP-Rh-Lip expressed VIP. CONCLUSIONS: Liposomes are specifically internalized by retinal Müller glial cells and resident macrophages in the eye. A limited passage of fluorescent liposomes from the vitreous to the spleen via the conventional outflow pathway and the venous circulation was detected. The majority of fluorescent liposomes deposited in the conjunctiva following IVT injection reached the subcapsular sinus of the cervical LN via conjuntival lymphatics. In the cervical LN, Rh-Lip were internalized by resident subcapsular sinus macrophages adjacent to T lymphocytes. Detection of VIP in both macrophages and T cells in cervical LN suggests that IVT injection of VIP-Rh-Lip may increase ocular immune privilege by modulating the loco-regional immune environment. In conclusion, our observations suggest that IVT injection of VIP-loaded liposomes is a promising therapeutic strategy to dampen ocular inflammation by modulating macrophage and T cell activation mainly in the loco-regional immune system.

Classical parameters of Shadowlands by Richard Attenborough (On C. S. Lewis's Life and Work)

The aim of this article is to show the classical parameters of Shadowlands by R. Attenborough, with a screenplay by W. Nicholson, on C. S. Lewis's life and work. Based upon an accurate reading of Lewis's works, the author of this article proposes to interpret the opposition Lewis / Gresham as the translation into the real life of the opposition between the Platonic or idealistic and the Aristotelian or materialistic temperaments which was already maintained by Coleridge. In any case, there are many classical references which must be taken into account in order to understand to what extent C. S. Lewis's Christianity is also a classic Christianity, that is, a Greek and Latin one.

Voyage autour du monde fait dans les années 1803, 1804, 1805 et 1806 par les ordres de sa majesté impériale Alexandre Ier, empereur de Russie, sur les vaisseaux "la Nadiejeda" et "la Neva", commandés par M. de Krusenstern ... traduit, de l'aveu et avec des additions de l'auteur ; la traduction revue par M. J.-B.-B. Eyriès...

Titre original : Reise um die Welt in den Jahren 1803, 1804, 1805 und 1806, auf Befehl seiner... Majestät Alexander des Ersten auf den Schiffen "Nadeshda" und "Newa"

Cocoa flavonoid-enriched diet modulates systemic and intestinal immunoglobulin synthesis in adult Lewis rats

Previous studies have reported that a diet containing 10% cocoa, a rich source of flavonoids, has immunomodulatory effects on rats and, among others effects, is able to attenuate the immunoglobulin (Ig) synthesis in both systemic and intestinal compartments. The purpose of the present study was focused on investigating whether these effects were attributed exclusively to the flavonoid content or to other compounds present in cocoa. To this end, eight-week-old Lewis rats were fed, for two weeks, either a standard diet or three isoenergetic diets containing increasing proportions of cocoa flavonoids from different sources: one with 0.2% polyphenols from conventional defatted cocoa, and two others with 0.4% and 0.8% polyphenols, respectively, from non-fermented cocoa. Diet intake and body weight were monitored and fecal samples were obtained throughout the study to determine fecal pH, IgA, bacteria proportions, and IgA-coated bacteria. Moreover, IgG and IgM concentrations in serum samples collected during the study were quantified. At the end of the dietary intervention no clear changes of serum IgG or IgM concentrations were quantified, showing few effects of cocoa polyphenol diets at the systemic level. However, in the intestine, all cocoa polyphenol-enriched diets attenuated the age-related increase of both fecal IgA and IgA-coated bacteria, as well as the proportion of bacteria in feces. As these effects were not dependent on the dose of polyphenol present in the diets, other compounds and/or the precise polyphenol composition present in cocoa raw material used for the diets could be key factors in this effect.

Studies of the association of the A, B and Lewis Blood group antigens with carcinoembryonic antigen (CEA).

Carcinoembryonic antigen (CEA) was purified from primary tumour or from hepatic metastases obtained from ten cases of carcinoma of the colon. In nine cases the blood group antigens A, B, Lea or Leb were detected in CEA preparations by the binding of 125I-labelled CEA by blood group antibodies. The extent of binding appeared to preclude simple contamination of CEA preparations by blood group glycoprotein. In all cases the blood group antigens detected were consistent with the patients' known blood groups. Blood group I and i activities were not detected. It is concluded that the determinants of A, B and Lewis antigens and of CEA share the same glycoprotein carrier molecules.

Das Landgut Monrepos in Finnland. 1804 / von Ludwig Heinrich von Nicolay.

Front.: port. of L. H. v. Nicolay by Auguste Maurin. -- Plates by J. Jacottet illustrating Monrepos and surroundings. -- Ornamented pages. -- Paperback.

Applications of optically active metallacycles: new useful materials for the determination of the enantiomeric excess of Lewis bases and as resolving agents for monodentate phosphines

Es descriu la síntesi de nous compostos ciclopal·ladats derivats d'imines i amines primàries, òpticament actius, així com les seves aplicacions per a la determinació de l'excés enantiomèric i per a la resolució de bases de Lewis. També es comenta la síntesi i resolució de noves fosfines P-quirals, així com la seva aplicació a processos de catàlisi asimètrica. A més, es descriu la preparació d'algunes fosfines secundàries quirals i s'estudia la seva estabilitat configuracional.

The Brazilian and patagonian fishes of the wilkes expedition: 1838-1842

Na viagem efetuada à volta do mundo pela U. S. Exploring Expedition mais conhecida pelo nome de Expedição Wilkes e durante o período em que a mesma operava no Atlântico Ocidental, foram visitadas as regiões do Rio de Janeiro e da Patagônia. Por ocasião da festa comemorativa do centenário da The American Philosophical Society, de Filadélfia, ocorrida a 23 de Fevereiro de 1930, o autor teve a oportunidade de apresentar uma nota prévia da qual constava uma lista de peixes capturados durante o cruzeiro da Expedição. Teve, então, o ensejo de examinar o diário redigido pelo Dr. Charles Pickering, botânico da Expedição, nele encontrando anotações valiosas para a História Natural, considerada em seu sentido amplo, detalhes a respeito das localidades percorridas e relações extensas de animais e plantas, além de diversos representantes pertencentes a outros grupos. Sob o ponto de vista histórico, os comentários aí constantes são de importância significativa. Em muitos casos, essas apreciações fornecem base sólida para a efetivação de estudos faunísticos, de caráter comparativo, muito instrutivos e úteis. O autor examinou a coleção de peixes da Expedição Wilkes que se encontra no Museu Nacional dos Estados Unidos, em Washington, acervo esse gentilmente posto à sua disposição para estudo. Dessa maneira, no presente trabalho, fornece matéria de amplo interesse histórico, visando com isso lançar alguma luz sobre a imensa fauna do Brasil e da Argentina. Baseando-se nos dados fornecidos por Pickering, o autor divulga dados curiosos a respeito da baía do Rio de Janeiro e adjacências, no ano de 1838. Segue-se uma lista contendo a diagnose de 82 espécimes de água doce, salobra e salgada, estudados e comparados com outros de várias procedências. Referindo-se ao Rio Negro, descreve o autor o aspeto da região patagônica, durante certo período de 1839, fazendo comentários a respeito de 7 espécies de peixes nela, encontradas.

A northern summer; or travels round the Baltic, through Denmark, Sweden, Russia, Prussia, and part of Germany in the year 1804 : by John Carr, esq.

Dedicated to: James Mackintosh.