Efeitos da maturação biológica sobre o custo energético, potência aeróbia, máximo déficit acumulado de oxigênio e performances de nadadores

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Associações entre, índices fisiológicos e fadiga neuromuscular com padrões de deslocamento e desempenho do chute de finalização no futsal

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comparison of maximal lactate steady state with V2, V4, individual anaerobic threshold and lactate minimum speed in horses

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A produção institucional na perspectiva arquivística: Lei de Acesso à Informação e aproximações

This study regards the implementation of the Law of Access to Information, from an archivistic perspective of the institutional production of recorded projects in the period 1992- 2012 at the Letters and Arts Center (LAC) of the Federal University of Santa Maria (UFSM) and stored on digital medium in its database. It concludes that the effects of the law of access to information, as related to the institutional production by UFSM, will bring benefits to all society.

Evaluation of cardiorespiratory and biochemical effects of ketamine-propofol and guaifenesin-ketamine-xylazine anesthesia in donkeys (Equus asinus)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo da interação genótipo ambiente em búfalos leiteiros do Brasil e da Colômbia

Pós-graduação em Zootecnia - FCAV

Aditivos alternativos aos antibióticos promotores de crescimento no desempenho de frangos de corte sob desafio sanitário

Pós-graduação em Zootecnia - FCAV

Down-regulation of SLC8A1 as a putative apoptosis evasion mechanism by modulation of calcium levels in penile carcinoma

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Regulation of glycogen metabolism by the CRE-1, RCO-1 and RCM-1 proteins in Neurospora crassa. The role of CRE-1 as the central transcriptional regulator

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Chromium or L-carnitine supplementation during an aerobic conditioning program mildly modified the energy metabolism biomarker response in Mangalarga Marchador fillies

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Comparação entre a recuperação da economia de corrida e força muscular medida por diferentes métodos após o dano muscular

The goal of the present study was to investigate possible differences towards strenght recovery measured by different methods and running economy (RE), after one session of downhill running (DR), in order to induce muscle damage (MD). Our hypothesis is that the strenght recovery measured by jumps is more alike RE markers, due to the measures' specificity. Ten male subjects, ages 18 to 30, apparently healthy, without any experience with strenght training at least 6 months before the tests took place in this study. After going to the lab twice (to familiarize themselves with the protocols, and determine their maximum oxygen consumption/running speeds), they went to the lab five times in the following week, in order to collect all the variables before, immediately after, and 24, 48, 72 and 96 hours in. The variables obtained were: stride frequency (SF), stride lenght (SL), oxygen volume (VO2), carbon dioxide volume (VCO2), ventilation (VE), lactate blood concentration ([Lac]), isometric peak torque (IPT), subjective pain perception (SPP), effort perception (EP), medial-portion circumference (CIR), knee movement extent (EXT), torque development rate (TDR), counter movement jump and squat jump height (HCM and HS), strenght development rate of both jumps (SDCM and SDS), and maximum soil strenght reaction (SRCM and SRS). Changes over time in all variables were verified by one way variancy analysis. Differences between the strenght measures were verified by two way variancy analysis. When significant effects were verified, Tuckey's post-hoc were applied. The significancy level taken on this study was p < 0,05. Of all indirect muscle damage markers, IPT, SPP and TDR were the only ones in which ocurred significant changes. We couldn't find the moments where this happened for TDR with the post-hoc used. On RE markers, VO2, [Lac] and VE suffered significant effects over time. About the jumps variables, only SDCM and HCM presented significant...

Parâmetros sanguíneos basais de cavalos submetidos à atividade aeróbica consumindo concentados lipídicos

The feeding diets were evaluated containing low and high levels of soybean oil for horses athletes subjected to two protocols of aerobic training on the response of basal blood biochemical parameters. Four horses were used in latin square design with treatments in a 2 x 2 factorial arrangement. Treatments consisted levels of 5 and 15% oil concentrates and two aerobic training, 40' and 60' minutes. Plasmatic parameters were monitored, triglyceride (TG), total cholesterol (TC), glucose (GLU) and lactate (LAC), during basal metabolism. The TG, TC, GLU and LAC from horses at rest were not affected (P> 0.05) neither of diet and physical activity, 0.21, 3.79, 4.18, 0.93 mmol L-1, respectively. It can be concluded that offer concentrate with high content of soybean oil to athletic horses in aerobic activities can be performed without altering the blood biochemical profile of basal metabolism.

RefZ Facilitates the Switch from Medial to Polar Division during Spore Formation in Bacillus subtilis

During sporulation, Bacillus subtilis redeploys the division protein FtsZ from midcell to the cell poles, ultimately generating an asymmetric septum. Here, we describe a sporulation-induced protein, RefZ, that facilitates the switch from a medial to a polar FtsZ ring placement. The artificial expression of RefZ during vegetative growth converts FtsZ rings into FtsZ spirals, arcs, and foci, leading to filamentation and lysis. Mutations in FtsZ specifically suppress RefZ-dependent division inhibition, suggesting that RefZ may target FtsZ. During sporulation, cells lacking RefZ are delayed in polar FtsZ ring formation, spending more time in the medial and transition stages of FtsZ ring assembly. A RefZ-green fluorescent protein (GFP) fusion localizes in weak polar foci at the onset of sporulation and as a brighter midcell focus at the time of polar division. RefZ has a TetR DNA binding motif, and point mutations in the putative recognition helix disrupt focus formation and abrogate cell division inhibition. Finally, chromatin immunoprecipitation assays identified sites of RefZ enrichment in the origin region and near the terminus. Collectively, these data support a model in which RefZ helps promote the switch from medial to polar division and is guided by the organization of the chromosome. Models in which RefZ acts as an activator of FtsZ ring assembly near the cell poles or as an inhibitor of the transient medial ring at midcell are discussed.

Characterization of the procera Tomato Mutant Shows Novel Functions of the SlDELLA Protein in the Control of Flower Morphology, Cell Division and Expansion, and the Auxin-Signaling Pathway during Fruit-Set and Development

procera (pro) is a tall tomato (Solanum lycopersicum) mutant carrying a point mutation in the GRAS region of the gene encoding SlDELLA, a repressor in the gibberellin (GA) signaling pathway. Consistent with the SlDELLA loss of function, pro plants display a GA-constitutive response phenotype, mimicking wild-type plants treated with GA(3). The ovaries from both nonemasculated and emasculated pro flowers had very strong parthenocarpic capacity, associated with enhanced growth of preanthesis ovaries due to more and larger cells. pro parthenocarpy is facultative because seeded fruits were obtained by manual pollination. Most pro pistils had exserted stigmas, thus preventing self-pollination, similar to wild-type pistils treated with GA(3) or auxins. However, Style2.1, a gene responsible for long styles in noncultivated tomato, may not control the enhanced style elongation of pro pistils, because its expression was not higher in pro styles and did not increase upon GA(3) application. Interestingly, a high percentage of pro flowers had meristic alterations, with one additional petal, sepal, stamen, and carpel at each of the four whorls, respectively, thus unveiling a role of SlDELLA in flower organ development. Microarray analysis showed significant changes in the transcriptome of preanthesis pro ovaries compared with the wild type, indicating that the molecular mechanism underlying the parthenocarpic capacity of pro is complex and that it is mainly associated with changes in the expression of genes involved in GA and auxin pathways. Interestingly, it was found that GA activity modulates the expression of cell division and expansion genes and an auxin signaling gene (tomato AUXIN RESPONSE FACTOR7) during fruit-set.

B Lymphocyte-Induced Maturation Protein-1 Contributes to Intestinal Mucosa Homeostasis by Limiting the Number of IL-17-Producing CD4(+) T Cells

The transcription factor B lymphocyte induced maturation protein-1 (Blimp-1) plays important roles in embryonic development and immunity. Blimp-1 is required for the differentiation of plasma cells, and mice with T cell specific deletion of Blimp-1 (Blimp-1CKO mice) develop a fatal inflammatory response in the colon. Previous work demonstrated that lack of Blimp-1 in CD4(+) and CD8(+) T cells leads to intrinsic functional defects, but little is known about the functional role of Blimp-1 in regulating differentiation of Th cells in vivo and their contribution to the chronic intestinal inflammation observed in the Blimp1CKO mice. In this study, we show that Blimp-1 is required to restrain the production of the inflammatory cytokine IL-17 by Th cells in vivo. Blimp-1CKO mice have greater numbers of IL-17 producing TCR beta(+)CD4(+)cells in lymphoid organs and in the intestinal mucosa. The increase in IL-17 producing cells was not restored to normal levels in wild-type and Blimp-1CKO mixed bone marrow chimeric mice, suggesting an intrinsic role for Blimp-1 in constraining the production of IL-17 in vivo. The observation that Blimp-1 deficient CD4(+) T cells are more prone to differentiate into IL-17(+)/IFN-gamma(+) cells and cause severe colitis when transferred to Rag1-deficient mice provides further evidence that Blimp-1 represses IL-17 production. Analysis of Blimp-1 expression at the single cell level during Th differentiation reveals that Blimp-1 expression is induced in Th1 and Th2 but repressed by TGF-beta in Th17 cells. Collectively, the results described here establish a new role for Blimp-1 in regulating IL-17 production in vivo. The Journal of Immunology, 2012,189: 5682-5693.