Red mangrove juvenile mangroves and mangrove peat soils at the wetland mesocosm facility. Plants were exposed to phosphorus addition and defolitation, mimicing effects of marine storms on coastal mangrove ecosystems.

http://digitalcommons.fiu.edu/fce_lter_photos/1334/thumbnail.jpg

Tolerance of juvenile Mytilus galloprovincialis to experimental seawater acidification

Coastal ocean acidification is expected to interfere with the physiology of marine bivalves. In this work, the effects of acidification on the physiology of juvenile mussels Mytilus galloprovincialis were tested by means of controlled CO2 perturbation experiments. The carbonate chemistry of natural (control) seawater was manipulated by injecting CO2 to attain 2 reduced pH levels: -0.3 and -0.6 pH units as compared with the control seawater. After 78 d of exposure, we found that the absorption efficiency and ammonium excretion rate of juveniles were inversely related to pH. Significant differences among treatments were not observed in clearance, ingestion and respiration rates. Coherently, the maximal scope for growth and tissue dry weight were observed in mussels exposed to the pH reduction delta pH=-0.6, suggesting that M. galloprovincialis could be tolerant to CO2 acidification, at least in the highly alkaline coastal waters of Ria Formosa (SW Portugal).

Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans

Recently, the prevalence of Fasciola hepatica in some areas has increased considerably and the availability of a vaccine to protect livestock from infection would represent a major advance in tools available for controlling this disease. To date, most vaccine-target discovery research on this parasite has concentrated on proteomic and transcriptomic approaches whereas little work has been carried out on glycosylation. As the F. hepatica tegument (Teg) may contain glycans potentially relevant to vaccine development and the Newly Excysted Juvenile (NEJ) is the first lifecycle stage in contact with the definitive host, our work has focused on assessing the glycosylation of the NEJTeg and identifying the NEJTeg glycoprotein repertoire. After in vitro excystation, NEJ were fixed and NEJTeg was extracted. Matrix-assisted laser desorption ionisation-time of flight-mass spectrometry (MALDI-TOF-MS) analysis of released N-glycans revealed that oligomannose and core-fucosylated truncated N-glycans were the most dominant glycan types. By lectin binding studies these glycans were identified mainly on the NEJ surface, together with the oral and ventral suckers. NEJTeg glycoproteins were affinity purified after targeted biotinylation of the glycans and identified using liquid chromatography and tandem mass spectrometry (LC-MS/MS). From the total set of proteins previously identified in NEJTeg, eighteen were also detected in the glycosylated fraction, including the F. hepatica Cathepsin B3 (FhCB3) and two of the Cathepsin L3 (FhCL3) proteins, among others. To confirm glycosylation of cathepsins, analysis at the glycopeptide level by LC-ESI-ion-trap-MS/MS with collision-induced dissociation (CID) and electron-transfer dissociation (ETD) was carried out. We established that cathepsin B1 (FhCB1) on position N80, and FhCL3 (BN1106_s10139B000014, scaffold10139) on position N153, carry unusual paucimannosidic Man2GlcNAc2 glycans. To our knowledge, this is the first description of F. hepatica NEJ glycosylation and the first report of N-glycosylation of F. hepatica cathepsins. The significance of these findings for immunological studies and vaccine development is discussed.

Stimulating Neoblast-Like Cell Proliferation in Juvenile Fasciola hepatica Supports Growth and Progression towards the Adult Phenotype In Vitro

Fascioliasis (or fasciolosis) is a socioeconomically important parasitic disease caused by liver flukes of the genus Fasciola. Flukicide resistance has exposed the need for new drugs and/or a vaccine for liver fluke control. A rapidly improving 'molecular toolbox' for liver fluke encompasses quality genomic/transcriptomic datasets and an RNA interference platform that facilitates functional genomics approaches to drug/vaccine target validation. The exploitation of these resources is undermined by the absence of effective culture/maintenance systems that would support in vitro studies on juvenile fluke development/biology. Here we report markedly improved in vitro maintenance methods for Fasciola hepatica that achieved 65% survival of juvenile fluke after 6 months in standard cell culture medium supplemented with 50% chicken serum. We discovered that this long-term maintenance was dependent upon fluke growth, which was supported by increased proliferation of cells resembling the "neoblast" stem cells described in other flatworms. Growth led to dramatic morphological changes in juveniles, including the development of the digestive tract, reproductive organs and the tegument, towards more adult-like forms. The inhibition of DNA synthesis prevented neoblast-like cell proliferation and inhibited growth/development. Supporting our assertion that we have triggered the development of juveniles towards adult-like fluke, mass spectrometric analyses showed that growing fluke have an excretory/secretory protein profile that is distinct from that of newly-excysted juveniles and more closely resembles that of ex vivo immature and adult fluke. Further, in vitro maintained fluke displayed a transition in their movement from the probing behaviour associated with migrating stage worms to a slower wave-like motility seen in adults. Our ability to stimulate neoblast-like cell proliferation and growth in F. hepatica underpins the first simple platform for their long-term in vitro study, complementing the recent expansion in liver fluke resources and facilitating in vitro target validation studies of the developmental biology of liver fluke.

Evidence from genetic and Lagrangian drifter data for transatlantic transport of small juvenile green turtles

[EN] Aim: A key life-history component for many animals is the need for movement between different geographical locations at particular times. Green turtle (Chelonia mydas) hatchlings disperse from their natal location to spend an early pelagic stage in the ocean, followed by a neritic stage where small juveniles settle in coastal areas. In this study, we combined genetic and Lagrangian drifter data to investigate the connectivity between natal and foraging locations. In particular, we focus on the evidence for transatlantic transport.

Variation in spatial distribution of juvenile loggerhead turtles in the eastern Atlantic and western Mediterranean Sea

[EN] Loggerhead sea turtles (Caretta caretta) originating from the Western Atlantic carry out one of the largest marine migrations, reaching the eastern Atlantic and Mediterranean Sea. It has been proposed that this transatlantic journey is simply a consequence of drifting, with the lack of a target destination and a passive dispersal with oceanic currents. This predicts that the size of the source populations and geographic distance to the feeding grounds should play important roles in defining stock composition in the eastern Atlantic and Mediterranean Sea.

Comparative study of hematologic and plasma biochemical variables in Eastern Atlantic juvenile and adult nesting loggerhead sea turtles (Caretta caretta).

[EN] Background: Plasma biochemical and hematologic variables are important in the management of endangered sea turtles, such as loggerheads. However, studies on blood biochemistry and hematology of loggerheads are limited, and different concentrations according to variable criteria have been reported. Objective: The purpose of this study was to establish and compare baseline plasma chemistry and hematology values in Eastern Atlantic juvenile and adult nesting loggerhead sea turtles (Caretta caretta).

Evidence from genetic and Lagrangian drifter data for transatlantic transport of small juvenile green turtles

[EN] Green turtle hatchlings disperse away from their natal location to spend an early pelagic stage in the ocean, followed by a neritic stage where small juveniles settle in coastal areas. Here, we combined genetic and Lagrangian drifter data to investigate the connectivity between natal and foraging locations; particularly focussing on the evidence for transatlantic transport. Our results supported the general hypothesis that turtles tend to select foraging areas ‘closest-to-home’.

The Adolescent Empathy Paradox and Juvenile Offending

Thesis (Master's)--University of Washington, 2016-08

Outcome measures for evaluating the effectiveness of juvenile justice programs

School-Based Referrals to the Juvenile Courts: Prevalence and Characteristics in Several States

Effective school discipline practices are essential to keeping schools safe and creating an optimal learning environment. However, the overreliance of exclusionary discipline often removes students from the school setting and deprives them of the opportunity to learn. Previous research has suggested that students are being introduced to the juvenile justice system through the use of school-based juvenile court referrals. In 2011, approximately 1.2 million delinquency cases were referred to the juvenile courts in the United States. Preliminary evidence suggests that an increasing number of these referrals have originated in the schools. This study investigated school-based referrals to the juvenile courts as an element of the School-to-Prison Pipeline (StPP). The likelihood of school-based juvenile court referrals and rate of dismissal of these referrals was examined in several states using data from the National Juvenile Court Data Archives. In addition, the study examined race and special education status as predictors of school-based juvenile court referrals. Descriptive statistics, logistic regression and odds ratio, were used to analyze the data, make conclusions based on the findings and recommend appropriate school discipline practices.

Influence of environmental conditions on the age, hatch dates, and growth of juvenile Atlantic menhaden in the Choptank River, MD

Since 1993 Atlantic menhaden has experienced sustained low juvenile production (recruitment) in the Chesapeake Bay. Factors controlling growth, abundance, and mortality of larval and juvenile menhaden change throughout ontogeny such that larval growth rates could carry over to juvenile growth and survival. The effects of winter thermal conditions on the hatch dates and growth of larval and juvenile Atlantic menhaden in Atlantic shelf and Chesapeake Bay habitats were examined using otolith (ear-stone) increment analyses and growth models. For 2010-2013, truncated hatch-date distributions provided evidence for a winter recruitment bottleneck in Atlantic menhaden caused by cold temperatures. Hatch-dates of surviving juveniles were skewed towards warmer months for years characterized by colder temperatures. Reduced larval growth rates, influenced by reduced temperature and food availability, carried over to juvenile growth rates. A growing degree-day model performed well in simulating observed juvenile growth rates in the Choptank River tributary of Chesapeake Bay.

Aspects of migration, size structure and mortality of juvenile Nile perch in the Mwanza Gulf of Lake Victoria

A detailed study of the early life of fishes forms one of the very important aspects with respect to recruitment mechanism and proper uneterstanding of the dynamics leading to sustainance of fish populations. It should be the central theme of the fisheries biologist and managers to extract the bilogical information relevant to the proper understanding of this part of the population. A number of studies in the Mwanza gulf and Lake Victoria have emphasized the need to monitor the fishery by conducting observations of stock size, migration, catch effort data and growth of big specimen (Acere 1981, Goudsward et al 1984, Asila. Ogari 1988 and Okemwa 1984). The present paper discusses the preliminary information on the size structure, mortality and migration of juvenile Nile perch. The long term objectives of the programme is aimed at describing the size structure, growth, mortality, recruitment pattern and population dynamics of the Nile perch up to 30 cm total length in the, Mwanza Gulf.

Bilateral Panuveitis at Etanercept Initiation for Juvenile Idiopathic Arthritis

Introduction: Uveitis is a well-known extra-rheumatological manifestation of juvenile idiopathic arthritis (JIA). Tumor necrosis factor has been used to treat uveitis associated with inflammatory diseases. A new onset uveitis under anti-TNF therapy is uncommon. Case presentation: A 12-years-old male, affected since the age of six years, by a severe form of polyarticular JIA. When etanercept was started, he presented panuveitis bilaterally, so we switched to infliximab with good response. Conclusions: The TNF soluble receptor could be considered as a possible promoter in inducing endogenous new onset uveitis in JIA.

Extensive Calcinosis in an Adult Nigerian Woman with Dermatomyositis

Objectives: Extensive calcinosis in a patient with dermatomyositis is a rare clinical presentation in adults. The aim of this report is to present the clinical and radiographic features of a 25-year-old woman with dermatomyositis and extensive calcinosis. Case Presentation: A 25-year-old woman with extensive calcinosis on background dermatomyositis is described. Results: The clinical and radiographic features of the patient were consistent with dermatomyositis complicated by extensive calcinosis in an adult. Conclusion: Dermatomyositis with extensive calcinosis is a rare clinical presentation in adults.