CHARACTERIZATION OF THE COLONIZATION FACTOR ANTIGEN II PLASMID (CFA/II) FROM ENTEROTOXIGENIC ESCHERICHIA COLI

The etiological role of enterotoxigenic E. coli (ETEC) in diarrheal diseases of man and domestic animals is firmly established. Besides the production of enterotoxins (ST and LT), ETEC produces other important virulence factors; the colonization factor antigens (CFAs). CFAs mediate the attachment of ETEC to the epithelial cells of the small intestine, and this favors colonization by the bacteria and facilitates delivery of the enterotoxins to the intestinal cells.^ The production of enterotoxin and CFA is determined by plasmids and has been found to be restricted to a select number of E. coli serotypes.^ In this work, plasmid DNA analysis was performed in twenty-three CFA/II-producing enterotoxigenic Escherichia coli strains and their spontaneous CFA/II-negative derivatives. In some cases, strains lost the high molecular weight plasmid and also the ability to produce CFA/II, ST and LT. In other cases there was a deletion of the plasmid, which produced strains that were CFA/II('-), ST('-), LT('-) or CFA/II('-), ST('+), LT('+).^ The CFA/II plasmid from strain PB-176 (06:H16:CFA/II('+), ST('+), LT('+)) was transferred by transformation into E. coli K12 with concomitant transfer of the three characteristics: CFA/II, ST and LT.^ A physical map of the prototype CFA/II:ST:LT (pMEP60) plasmid was constructed by restriction endonuclease analysis and compared to plasmids from three other CFA/II-producing strains. A CFA/II-negative (but ST and LT positive) deletion derivative of pMEP60 (pMEP30) was also included in the map. The four CFA/II plasmids analyzed had a common region of approximately 30 kilobase pairs. The toxin genes were approximately 5 kbp apart and about 20 kbp from the common region. The information given by this physical map could be of great value when constructing a clone that will express the CFA/II genes but not the toxin genes. ^

Analysis of a disaster medical track for the Certificate in Emergency Management and Preparedness Program at the University of Texas at Dallas

Since the tragic events of September 11, 2001, the United States has engaged in building the infrastructure and developing the expertise necessary to protect its borders and its citizens from further attacks against its homeland. One approach has been the development of academic courses to educate individuals on the nature and dangers of subversive attacks and to prepare them to respond to attacks and other large-scale emergencies in their roles as working professionals, participating members of their communities, and collaborators with first responders. An initial review of the literature failed to reveal any university-based emergency management courses or programs with a disaster medical component, despite the public health significance and need for such programs. In the Fall of 2003, The School of Management at The University of Texas at Dallas introduced a continuing education Certificate in Emergency Management and Preparedness Program. This thesis will (1) describe the development and implementation of a new Disaster Medical Track as a component of this Certificate in Emergency Management and Preparedness Program, (2) analyze the need for and effectiveness of this Disaster Medical Track, and (3) propose improvements in the track based on this analysis. ^

Determinants of CD4 cell count decline after treatment interruption

Patients who had started HAART (Highly Active Anti-Retroviral Treatment) under previous aggressive DHHS guidelines (1997) underwent a life-long continuous HAART that was associated with many short term as well as long term complications. Many interventions attempted to reduce those complications including intermittent treatment also called pulse therapy. Many studies were done to study the determinants of rate of fall in CD4 count after interruption as this data would help guide treatment interruptions. The data set used here was a part of a cohort study taking place at the Johns Hopkins AIDS service since January 1984, in which the data were collected both prospectively and retrospectively. The patients in this data set consisted of 47 patients receiving via pulse therapy with the aim of reducing the long-term complications. ^ The aim of this project was to study the impact of virologic and immunologic factors on the rate of CD4 loss after treatment interruption. The exposure variables under investigation included CD4 cell count and viral load at treatment initiation. The rates of change of CD4 cell count after treatment interruption was estimated from observed data using advanced longitudinal data analysis methods (i.e., linear mixed model). Using random effects accounted for repeated measures of CD4 per person after treatment interruption. The regression coefficient estimates from the model was then used to produce subject specific rates of CD4 change accounting for group trends in change. The exposure variables of interest were age, race, and gender, CD4 cell counts and HIV RNA levels at HAART initiation. ^ The rate of fall of CD4 count did not depend on CD4 cell count or viral load at initiation of treatment. Thus these factors may not be used to determine who can have a chance of successful treatment interruption. CD4 and viral load were again studied by t-tests and ANOVA test after grouping based on medians and quartiles to see any difference in means of rate of CD4 fall after interruption. There was no significant difference between the groups suggesting that there was no association between rate of fall of CD4 after treatment interruption and above mentioned exposure variables. ^

AN INVESTIGATION OF THE RELIABILITY AND VALIDITY OF THE CENTER FOR EPIDEMIOLOGIC STUDIES DEPRESSION SCALE IN THREE ETHNIC GROUPS

Epidemiologic studies of mental disorder have called attention to the need for identifying untreated cases and to the inadequacies of the instruments available for this purpose. Accurate case ascertainment devices are the basis of sound epidemiology. Without these, neither case classification nor analytic studies of risk factors is possible.^ The purpose of this research was to examine the reliability and validity of an instrument designed to measure depressive symptoms in community populations--the Center for Epidemiologic Studies Depression Scale (CES-D Scale). Two particular foci of the study were whether or not the scale had the same statistical structure across three ethnic groups and whether or not the magnitude and pattern of rates of symptoms for these groups were affected by one source of response error, that due to response tendencies. The effects of age and education on the pattern and magnitude of rates also were examined. In addition, the reliability and validity of the measures of response tendencies were assessed.^ The study population consisted of residents of Alameda County, California. A stratified sample of approximately 700 whites, blacks and Mexican-Americans was interviewed in the summer and fall of 1978.^ The results of the analysis indicated that the scale was reliable and measured a similar content domain across the three ethnic groups. The unadjusted sex- and ethnic-specific rates of depressive symptoms showed an ethnic pattern for both sexes: rates for whites were lowest, those for Mexican-Americans were highest, and those for blacks were intermediate. Measures of response tendencies--need for social approval, trait desirability, and acquiescence--affected the magnitude of the rates for most comparisons. Likewise, the pattern of rates changed somewhat from that originally observed. The one fairly consistent observation was that rates for Mexican-American women were higher than those for the other two female subgroups in most of the comparisons. These results must be considered in the context of the reliability and validity assessment of the measures of response tendencies which indicated the tenuousness of these measures.^ Age affected the ethnic pattern of rates for men in an inconsistent way; for women, Mexican-Americans continued to have higher rates than whites or blacks in all age categories. Education affected the magnitude of rates for women but not for men. For both men and women, Mexican-Americans had higher rates in all educational strata. Rates for women showed an inverse association with education while those for men did not. ^

ATTITUDES TOWARD AND UTILIZATION OF NON-INVASIVE PRENATAL TESTING FOR CHROMOSOME ANEUPLOIDY AMONG OB/GYNS

Prenatal diagnosis is traditionally made via invasive procedures such as amniocentesis and chorionic villus sampling (CVS). However, both procedures carry a risk of complications, including miscarriage. Many groups have spent years searching for a way to diagnose a chromosome aneuploidy without putting the fetus or the mother at risk for complications. Non-invasive prenatal testing (NIPT) for chromosome aneuploidy became commercially available in the fall of 2011, with detection rates similar to those of invasive procedures for the common autosomal aneuploidies (Palomaki et al., 2011; Ashoor et al. 2012; Bianchi et al. 2012). Eventually NIPT may become the diagnostic standard of care and reduce invasive procedure-related losses (Palomaki et al., 2011). The integration of NIPT into clinical practice has potential to revolutionize prenatal diagnosis; however, it also raises some crucial issues for practitioners. Now that the test is clinically available, no studies have looked at the physicians that will be ordering the testing or referring patients to practitioners who do. This study aimed to evaluate the attitudes of OB/GYN’s and how they are incorporating the test into clinical practice. Our study shows that most physicians are offering this new, non-invasive technology to their patients, and that their practices were congruent with the literature and available professional society opinions. Those physicians who do not offer NIPT to their patients would like more literature on the topic as well as instructive guidelines from their professional societies. Additionally, this study shows that the practices and attitudes of MFMs and OBs differ. Our population feels that the incorporation of NIPT will change their practices by lowering the amount of invasive procedures, possibly replacing maternal serum screening, and that it will simplify prenatal diagnosis. However, those physicians who do not offer NIPT to their patients are not quite sure how the test will affect their clinical practice. From this study we are able to glean how physicians are incorporating this new technology into their practice and how they feel about the addition to their repertoire of tests. This knowledge gives insight as to how to best move forward with the quickly changing field of prenatal diagnosis.

Quality of life and functional status in a low -income, multiethnic HIV -positive population

The introduction of new medical treatments in recent years, commonly referred to as highly active antiretroviral therapy, has greatly increased the survival of patients with HIV/AIDS. As patients with HIV/AIDS continue to live longer, other important health-related outcomes, such as quality of life (QOL), should be thoroughly studied. There is also evidence that racial/ethnic minorities are disproportionately affected by HIV/AIDS, but potential health disparities among individuals already infected with HIV/AIDS have not been adequately examined in ethnically diverse populations. The purpose of this dissertation was to: (1) examine the impact of both demographic and behavioral variables on functional status and overall QOL among a population of ethnically diverse and economically disadvantaged HIV/AIDS patients; (2) examine the psychometric properties of a functional status measure—the Household and Leisure Time Activities questionnaire (HLTA); and (3) assess a proximal-distal theoretical framework for QOL using a full structural equation model in a population of patients with HIV/AIDS. Analyses were performed using data collected in the fall of 2000 from the project, Health and Work-Related Quality of Life and Health Risk Behaviors in a Multiethnic HIV-positive Population . Investigators from The University of Texas M.D. Anderson Cancer Center, The University of Texas-Houston Medical School, and The University of Texas School of Public Health conducted this project. The study site was the Thomas Street Clinic (TSC), a comprehensive HIV/AIDS care facility funded by the Harris County Hospital District (HCHD). TSC provides HIV/AIDS care to a diverse population of approximately 4000 medically indigent residents of Harris County. A systematic, consecutive sampling procedure yielded a sample size of 348 patients. Findings suggested that overall QOL, work-role functioning, household functioning, and leisure time functioning were impaired in this patient population. Results from the psychometric evaluation indicated that the HLTA was a reliable and valid measure of household and leisure time functioning status in a low-income multiethnic HIV-positive population. Finally, structural equation modeling of the proximal-distal QOL model suggested that this model was not a viable representation of the relationship between the study variables in this patient population. ^

Self, writing and feminine transactions in Virginia Woolf's Orlando and Clarice Lispector’s The stream of life

Este artículo realiza un acercamiento a Orlando, de Virginia Woolf, y a Agua viva, de Clarice Lispector, como obras que logran escribir la multiplicidad, la fluidez y la contingencia del ser. Jugando con las convenciones de la biografía y la autobiografía respectivamente, estas obras encuentran los medios para presentar un sujeto multidimensional y para mostrar, en particular, cómo la dimensión relacional, forjada por un orden simbólico patriarcal, ha hecho de la mujer el “otro" del hombre; un “otro" que debe ser dominado. Se exploran en este artículo los conceptos de “economía masculina" y “economía femenina" teorizados por Hélène Cixous y se propone al lenguaje poético como un medio capaz de eludir los dictados del falocentrismo. Lo poético, que no puede ser nunca agotado por uno o varios sistemas de significación, brinda la posibilidad de ir más allá de las categorizaciones y de explorar la multiplicidad. Tanto Orlando como Agua viva muestran las estrategias y las esperanzas de personajes y escritoras que ven en el lenguaje poético, en la “escritura femenina" como la entiende Cixous, el potencial de desarticular la “economía masculina" y abrir un nuevo espacio para sujetos diversos, múltiples y complejos.

Multi-proxy analyses of sediment cores from the Pakistan continental margin

Late Holocene laminated sediments from a core transect centred in the oxygen minimum zone (OMZ) impinging at the continental slope off Pakistan indicate stable oxygen minimum conditions for the past 7000 calendar years. High SW-monsoon-controlled biological productivity and enhanced organic matter preservation during this period is reflected in high contents of total organic carbon (TOC) and redox-sensitive elements (Ni, V), as well as by a low-diversity, high-abundance benthic foraminiferal Buliminacea association and high abundance of the planktonic species Globigerina bulloides indicative of upwelling conditions. Surface-water productivity was strongest during SW monsoon maxima. Stable OMZ conditions (reflected by laminated sediments) were found also during warm interstadial events (Preboreal, Bølling-Allerød, and Dansgaard-Oeschger events), as well as during peak glacial times (17-22.5 ka, all ages in calendar years). Sediment mass accumulation rates were at a maximum during the Preboreal and Younger Dryas periods due to strong riverine input and mobilisation of fine-grained sediment coinciding with rapid deglacial sea-level rise, whereas eolian input generally decreased from glacial to interglacial times. In contrast, the occurrence of bioturbated intervals from 7 to 10.5 ka (early Holocene), in the Younger Dryas (11.7-13 ka), from 15 to 17 ka (Heinrich event 1) and from 22.5 to 25 ka (Heinrich event 2) suggests completely different conditions of oxygen-rich bottom waters, extremely low mass and organic carbon accumulation rates, a high-diversity benthic fauna, all indicating lowered surface-water productivity. During these intervals the OMZ was very poorly developed or absent and a sharp fall of the aragonite compensation depth favoured the preservation of pteropods. The abundance of lithogenic proxies suggests aridity and wind transport by northwesterly or northeasterly winds during these periods coinciding with the North Atlantic Heinrich events and dust peaks in the Tibetan Loess records. The correlation of the monsoon-driven OMZ variability in the Arabian Sea with the rapid climatic fluctuations in the high northern latitudes suggests a close coupling between the climates of the high and low latitudes at a global scale.

Autosuperación del Hombre

Según Nietzsche, a partir de la Revolución Francesa tenemos que estar preparados para 'conmociones fundamentales'; desde entonces se producen acontecimientos que anuncian modificaciones infra y superestructurales para el próximo milenio, con lo cual, el orden social se ajustará al rumbo de las ?nuevas ideas?. En la actualidad, los triunfos y logros de la civilización ponen en peligro el desarrollo mismo de la cultura; son tiempos de procesos políticos extraordinarios o en otras palabras, de grandes luchas por el dominio de la tierra. Es así que se aproxima el 'ocaso de los ídolos', es decir, la ruina de las verdades antiguas pues casi todo lo que hasta el presente se llama verdad es sólo mentira y, cuando la nueva realidad se enfrente a las fabulaciones milenarias, nuestra cosmovisión sufrirá terribles sacudidas; las guerras serán inevitables y todas las instituciones basadas en el engaño y la ilusión caerán ante el asombro de los sobrevivientes. Nietzsche inicia la desacralización o 'desenmascaramiento' de las verdades eternas y la transmutación de todos los valores que son, en definitiva, procesos ineluctables. Asimismo, no hay fenómenos morales sino una 'interpretación moral de los fenómenos' o, en todo caso, una falsa interpretación. Y la moral no es otra cosa que una coacción prolongada. En la medida en que la Humanidad se aleja de la fe, más se acerca a la Ciencia, porque mientras la primera significa no querer saber qué es la verdad, la segunda nos asemeja a Dios. Según Nietzsche, estamos en ese camino. Día tras día el Hombre se perfecciona mediante la investigación de la Naturaleza; forma un cuerpo físico, mental y espiritual cada vez más enaltecido, realiza experimentos para mejorar la nutrición, el modo de vivir, la manera de interpretar los fenómenos de la Naturaleza y la expansión de la conciencia; pero en última instancia, no se trata del Hombre, pues éste tiene que ser superado. En consecuencia con lo dicho, el propósito del presente trabajo consiste en brindar una interpretación de la propuesta nietzscheana de 'Autosuperación del Hombre' en relación con los diferentes períodos de la vida intelectual de Nietzsche; la misma se inscribe en el marco de una teoría política contraria a las doctrinas antes mencionadas: la filosofía de la historia hegeliana, el socialismo, el nacionalismo, el anarquismo, la democracia y el cristianismo

Las leyes sobre vagancia : Control policial y práctica judicial en el ocaso de la frontera (Tandil 1872-1881)

A fines de la década de 1870, el gobierno provincial, con el objeto de lograr mayor control social sobre la población rural, implementó una reestructuración policial en la campaña bonaerense. La misma tenía por objetivo afianzar la propiedad privada y contribuir al mercado de trabajo, asimismo perseguía un fuerte interés en satisfacer la necesidad del estado de hombres en plena etapa de expansión de la frontera. La presión reclutadora del estado se reflejará en un aumento de detenidos por vagancia, dado que las leyes de represión de la vagancia del Código Rural permitían su penalización al servicio de las armas. El Código Rural sancionado en 1865 estipulaba dos normas procesales a cumplir en los acusados: formación de un sumario y constitución de un jury para su juzgamiento. Disposiciones que raramente se pusieron en práctica por los jueces de paz desde que esta legislación entró en vigencia. Será a fines de la década de 1870 cuando el gobierno exigirá más firmemente no solo la persecución de la vagancia sino también la aplicación de estas normas procesales en los acusados. Aún así, el análisis de la práctica judicial revelará que continuará existiendo una constante manipulación normativa sobre la base de dos valores que se intentaban afianzar, el trabajo y la propiedad. En los perjudiciales que atentaran contra estos dos valores, será en quienes se manipulará la normativa sancionada y se canalizará la presión reclutadora.

Rewriting the history of the tyrannicides : Thucydides versus Herodotus?

Este trabajo trata sobre la famosa digresión de Tucídides en el libro sexto de su historia acerca de la caída de la tiranía en Atenas (Tuc. 6.54-59) y su relación con el relato de Heródoto. La digresión de Tucídides (y más específicamente su tono polémico) ha provocado controversia entre los comentadores, que han analizado a fondo las narrativas de los dos historiadores tanto desde una perspectiva histórica como historiográfica. Este estudio tiende a contribuir a esta discusión a través de tres sugerencias: la primera, Tucídides se mete no solo con la pequeña sección sobre los tiranicidas de la Historia de Heródoto (esto es Hdt. 5.55-65), sino, más bien, con toda la narrativa de la Historia de Heródoto sobre la liberación de Atenas de la tiranía que se extiende hasta el discurso de Socles (esto es Hdt. 5.55-5.96.2); segunda, las correcciones de Tucídides al relato de Heródoto son menores, tercera, dado que las divergencias de Tucídides con respecto a Heródoto no son decisivas para la versión correcta de los hechos, el tono polémico de Tucídides en su digresión resulta todavía más difícil de explicar. En este trabajo se sugiere tentativamente que la actitud polémica de Tucídides tiene más sentido si es interpretada en el contexto de la rivalidad del historiador con Heródoto

Crises of social significances: adolescents and their projects for future work

This is a report on data raised through research, which has already finished, about Identification references in adolescents . Objetives : To analyze and reflect upon the collected data, adolescents' representations about the work field and their projects for future work. To conceptualize about the crises of social significances which produce and order sense and, therefore, organize our perceptions of work through social discourses and practices which have an impact on the subjectivity of adolescents and young adults. Methodology: Brief presentation of the concepts which frame the qualitative research in this area. Reading and analysis of fragments from adolescents' accounts, obtained in focal groups at different state secondary schools. Conclusions: The adolescents researched portray a self-image full of uncertainty and skepticism as regards their future possibilities. The impact produced by the fall of work as an ideal of doing and being is made evident in their accounts. Only some of them link their future project with an invested and valuable activity, even if this is not associated with economic safety and wellbeing. Others present themselves as incapable to modify the state of things, which offers a field connected with consumerist values and with the idea that "you are worth what you have (in material things)".

Autosuperación del Hombre

Según Nietzsche, a partir de la Revolución Francesa tenemos que estar preparados para 'conmociones fundamentales'; desde entonces se producen acontecimientos que anuncian modificaciones infra y superestructurales para el próximo milenio, con lo cual, el orden social se ajustará al rumbo de las ?nuevas ideas?. En la actualidad, los triunfos y logros de la civilización ponen en peligro el desarrollo mismo de la cultura; son tiempos de procesos políticos extraordinarios o en otras palabras, de grandes luchas por el dominio de la tierra. Es así que se aproxima el 'ocaso de los ídolos', es decir, la ruina de las verdades antiguas pues casi todo lo que hasta el presente se llama verdad es sólo mentira y, cuando la nueva realidad se enfrente a las fabulaciones milenarias, nuestra cosmovisión sufrirá terribles sacudidas; las guerras serán inevitables y todas las instituciones basadas en el engaño y la ilusión caerán ante el asombro de los sobrevivientes. Nietzsche inicia la desacralización o 'desenmascaramiento' de las verdades eternas y la transmutación de todos los valores que son, en definitiva, procesos ineluctables. Asimismo, no hay fenómenos morales sino una 'interpretación moral de los fenómenos' o, en todo caso, una falsa interpretación. Y la moral no es otra cosa que una coacción prolongada. En la medida en que la Humanidad se aleja de la fe, más se acerca a la Ciencia, porque mientras la primera significa no querer saber qué es la verdad, la segunda nos asemeja a Dios. Según Nietzsche, estamos en ese camino. Día tras día el Hombre se perfecciona mediante la investigación de la Naturaleza; forma un cuerpo físico, mental y espiritual cada vez más enaltecido, realiza experimentos para mejorar la nutrición, el modo de vivir, la manera de interpretar los fenómenos de la Naturaleza y la expansión de la conciencia; pero en última instancia, no se trata del Hombre, pues éste tiene que ser superado. En consecuencia con lo dicho, el propósito del presente trabajo consiste en brindar una interpretación de la propuesta nietzscheana de 'Autosuperación del Hombre' en relación con los diferentes períodos de la vida intelectual de Nietzsche; la misma se inscribe en el marco de una teoría política contraria a las doctrinas antes mencionadas: la filosofía de la historia hegeliana, el socialismo, el nacionalismo, el anarquismo, la democracia y el cristianismo

Las leyes sobre vagancia : Control policial y práctica judicial en el ocaso de la frontera (Tandil 1872-1881)

A fines de la década de 1870, el gobierno provincial, con el objeto de lograr mayor control social sobre la población rural, implementó una reestructuración policial en la campaña bonaerense. La misma tenía por objetivo afianzar la propiedad privada y contribuir al mercado de trabajo, asimismo perseguía un fuerte interés en satisfacer la necesidad del estado de hombres en plena etapa de expansión de la frontera. La presión reclutadora del estado se reflejará en un aumento de detenidos por vagancia, dado que las leyes de represión de la vagancia del Código Rural permitían su penalización al servicio de las armas. El Código Rural sancionado en 1865 estipulaba dos normas procesales a cumplir en los acusados: formación de un sumario y constitución de un jury para su juzgamiento. Disposiciones que raramente se pusieron en práctica por los jueces de paz desde que esta legislación entró en vigencia. Será a fines de la década de 1870 cuando el gobierno exigirá más firmemente no solo la persecución de la vagancia sino también la aplicación de estas normas procesales en los acusados. Aún así, el análisis de la práctica judicial revelará que continuará existiendo una constante manipulación normativa sobre la base de dos valores que se intentaban afianzar, el trabajo y la propiedad. En los perjudiciales que atentaran contra estos dos valores, será en quienes se manipulará la normativa sancionada y se canalizará la presión reclutadora.

Rewriting the history of the tyrannicides : Thucydides versus Herodotus?

Este trabajo trata sobre la famosa digresión de Tucídides en el libro sexto de su historia acerca de la caída de la tiranía en Atenas (Tuc. 6.54-59) y su relación con el relato de Heródoto. La digresión de Tucídides (y más específicamente su tono polémico) ha provocado controversia entre los comentadores, que han analizado a fondo las narrativas de los dos historiadores tanto desde una perspectiva histórica como historiográfica. Este estudio tiende a contribuir a esta discusión a través de tres sugerencias: la primera, Tucídides se mete no solo con la pequeña sección sobre los tiranicidas de la Historia de Heródoto (esto es Hdt. 5.55-65), sino, más bien, con toda la narrativa de la Historia de Heródoto sobre la liberación de Atenas de la tiranía que se extiende hasta el discurso de Socles (esto es Hdt. 5.55-5.96.2); segunda, las correcciones de Tucídides al relato de Heródoto son menores, tercera, dado que las divergencias de Tucídides con respecto a Heródoto no son decisivas para la versión correcta de los hechos, el tono polémico de Tucídides en su digresión resulta todavía más difícil de explicar. En este trabajo se sugiere tentativamente que la actitud polémica de Tucídides tiene más sentido si es interpretada en el contexto de la rivalidad del historiador con Heródoto