Discrimination of bile acids by the rainbow trout olfactory system: evidence as potential pheromone

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Action of the chemical agent fipronil on the reproductive process of semi-engorged females of the tick Rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae). Ultrastructural evaluation of ovary cells

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Proteomic Analysis of Urine in Rats Chronically Exposed to Fluoride

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The role of the seed coat in phytochrome-controlled seed germination in Lactuca sativa L. cv. Grand Rapids

The effect of scarification with sodium hypochloride on light sensitivity in seeds of Lactuca sativa L. cv. Grand Rapids is presented in the paper. Light-requiring lettuce seeds germinated in both dark and continuous light after scarification and 36 degrees C pre-incubation restored light sensitivity at 25 degrees C. Curves of dose-response indicated that chemical scarification induced a change in the control of seed germination from Low Fluence Response to the Very Low Fluence Response. Pre-incubation at 36 degrees C return the control to the Low Fluence Response of phytochrome action.

The effect of acidic residues and amphipathicity on the lytic activities of mastoparan peptides studied by fluorescence and CD spectroscopy

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effects of malathion and cadmium on acetylcholinesterase activity and metallothionein levels in the fish Seriola dumerilli

The potential use of acetylcholinesterase (AChE) and metallothionein (MT) responses as biomarker of organophosphorous (OPs) and trace metal were assessed in fish Seriola dumerilli exposed to 0, 4, 6 mg/kg of malathion for 2, 7 and 13 days, and to 0, 50, 100, 250 mu g/kg of Cd for 2 days. Brain AChE was significantly inhibited after 2 and 7 days of malathion exposure, in a dose-response manner, but no inhibition was observed after 13 days of exposure. When exposed to Cd for 2 days, S. dumerelli presented an increase in AChE activity at a concentration of 50 mu g/kg, but a strong and dose-dependent AChE inhibition at 100 and 250 mu g/kg. Cd treatment also caused a rapid increase in MTs concentration in liver, even at the lower concentration. Our experiments indicate that the measurement of hepatic MT concentration and brain AChE activity in S. dumerilli would be useful biomarkers of OP and Cd exposure and/or effects.

Indole ring oxidation by activated leukocytes prevents the production of hypochlorous acid

Hypochlorous acid (HOCl) released by activated leukocytes has been implicated in the tissue damage that characterizes chronic inflammatory diseases. In this investigation, 14 indole derivatives, including metabolites such as melatonin, tryptophan and indole-3-acetic acid, were screened for their ability to inhibit the generation of this endogenous oxidant by stimulated leukocytes. The release of HOCl was measured by the production of taurine-chloramine when the leukocytes (2 x 10(6) cells/mL) were incubated at 37ºC in 10 mM phosphate-buffered saline, pH 7.4, for 30 min with 5 mM taurine and stimulated with 100 nM phorbol-12-myristate acetate. Irrespective of the group substituted in the indole ring, all the compounds tested including indole, 2-methylindole, 3-methylindole, 2,3-dimethylindole, 2,5-dimethylindole, 2-phenylindole, 5-methoxyindole, 6-methoxyindole, 5-methoxy-2-methylindole, melatonin, tryptophan, indole-3-acetic acid, 5-methoxy-2-methyl-3-indole-acetic acid, and indomethacin (10 µM) inhibited the chlorinating activity of myeloperoxidase (MPO) in the 23-72% range. The compounds 3-methylindole and indole-3-acetic acid were chosen as representative of indole derivatives in a dose-response study using purified MPO. The IC50 obtained were 0.10 ± 0.03 and 5.0 ± 1.0 µM (N = 13), respectively. These compounds did not affect the peroxidation activity of MPO or the production of superoxide anion by stimulated leukocytes. By following the spectral change of MPO during the enzyme turnover, the inhibition of HOCl production can be explained on the basis of the accumulation of the redox form compound-II (MPO-II), which is an inactive chlorinating species. These results show that indole derivatives are effective and selective inhibitors of MPO-chlorinating activity.

Burnt sugarcane harvesting: Particulate matter exposure and the effects on lung function, oxidative stress, and urinary 1-hydroxypyrene

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Isobutyl amides - potent compounds for controlling Diatraea saccharalis

BACKGROUND: A dichloromethane-methanol extract of the seeds of Piper tuberculatum Jacq. (Piperaceae) and two isobutyl amides, 4,5-dihydropiperlonguminine (1) and pellitorine (2), which were isolated by chromatographic methods, were assayed for their lethality against the sugarcane borer Diatraea saccharalis F. (Lepidoptera: Pyralidae).RESULTS: Bioassays were carried out with fourth-instar caterpillars through topical application of test solutions to the dorsal surface of the prothorax, and dose-response correlations were determined. Significant insect mortalities were observed 24, 48 and 72 h after treatment at concentrations of >= 100 mu g insect(-1). The LD(50) and LD(90) values for compound 1 were 92.83 and 176.50 mu g insect(-1), and for compound 2 they were 91.19 and 184.56 mu g insect(-1).CONCLUSION: According to the LD(50) and LD(90) for compounds 1 and 2, it can be inferred that the values reflect an acute lethal response to both compounds, based on interaction(s) of the toxicants with a primary target or series of targets. Thus, the amides were demonstrated to have potential value in the control of the sugarcane borer. (C) 2008 Society of Chemical Industry

LOW-PROTEIN DIET IMPAIRS GLUCOSE-INDUCED INSULIN-SECRETION FROM AND CA-45 UPTAKE BY PANCREATIC RAT ISLETS

Glucose-induced insulin secretion rom and Ca-45 uptake by isolated pancreatic islets, derived from rats fed with normal (NPD) or low protein diet (LPD), were studied. Insulin secretion from both types of islets in response to increasing concentrations of glucose followed an S-shaped pattern. However, basal secretion observed at substimulatory concentrations of glucose (0-5.6 mM), as well as maximal release, obtained at 16.7 mM or higher glucose concentrations were significantly reduced in islets from LPD. Furthermore, in LPD rat islets, the dose-response curve to glucose was clearly shifted to the right compared with NPD islets, with the half-maximal response occurring at 8.5 and 14.4 mM glucose for NPD and LPD islets, respectively. In islets from NPD rats, the Ca-45 content, after 5 or 90 min in the presence of 8.3 mM glucose, was higher than that observed for islets kept at 2.8 mM glucose and increased further at 16.7 mM glucose. After 5 min of incubation, the Ca-45 uptake by LPD islets in the presence of 8.3 mM glucose was slightly higher than basal values (2.8 mM glucose); however, no further increase in the Ca-45 uptake was noticed at 16.7 mM glucose. In LPD islets a significant increase in Ca-45 uptake over basal values was registered only at 16.7 mM glucose, after 90 min of incubation. These data indicate that the poor secretary response to glucose observed in islets from LPD rats may be related to a defect in the ability of glucose to increase Ca2+ uptake and/or to reduce Ca2+ efflux from beta-cells.

Weed seed-germination .3. bidens-pilosa L

The germination of seeds of Bidens pilosa L. colected at different places was analyzed by dose-response curves for induction and reversion of germination in seeds pre-incubated at 25 degrees C or 36 degrees C during 24 hours. Our results indicate that phytochrome is responsible for the control of seed germination id Bidens pilosa and the level of pre-existing active form of phytochrome (Pfr) above the threshold switch the seed to germinate in darkness.

Pulmonary neutrophil recruitment and bronchial reactivity in formaldehyde-exposed rats are modulated by mast cells and differentially by neuropeptides and nitric oxide

We have used a pharmacological approach to study the mechanisms underlying the rat lung injury and the airway reactivity changes induced by inhalation of formaldehyde (FA) (1% formalin solution, 90 min once a day, 4 days). The reactivity of isolated tracheae and intrapulmonary bronchi were assessed in dose-response curves to methacholine (MCh). Local and systemic inflammatory phenomena were evaluated in terms of leukocyte countings in bronchoalveolar lavage (BAL) fluid, blood, bone marrow lavage and spleen. Whereas the tracheal reactivity to MCh did not change, a significant bronchial hyporesponsiveness (BHR) was found after FA inhalation as compared with naive rats. Also, FA exposure significantly increased the total cell numbers in BAL, in peripheral blood and in the spleen, but did not modify the counts in bone marrow. Capsaicin hindered the increase of leukocyte number recovered in BAL fluid after FA exposure. Both compound 48/80 and indomethacin were able to prevent the lung neutrophil influx after FA, but indomethacin had no effect on that of mononuclear cells. Following FA inhalation, the treatment with sodium cromoglycate (SCG), but not with the nitric oxide (NO) synthase inhibitor L-NAME, significantly reduced the total cell number in BAL. Compound 48/80, L-NAME and SCG significantly prevented BHR to MCh after FA inhalation, whereas capsaicin was inactive in this regard. on the other hand, indomethacin exacerbated BHR. These data suggest that after FA inhalation, the resulting lung leukocyte influx and BHR may involve nitric oxide, airway sensory fibers and mast cell-derived mediators. The effect of NO seemed to be largely restricted to the bronchial tonus, whereas neuropeptides appeared to be linked to the inflammatory response, therefore indicating that the mechanisms responsible for the changes of airway responsiveness caused by FA may be separate from those underlying its inflammatory lung effects. (c) 2005 Elsevier B.V. All rights reserved.

Detection of Sourgrass (Digitaria insularis) Biotypes Resistant to Glyphosate in Brazil

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Popcorn (Zea mays L. var. everta) yield and yield components as influenced by the timing of broadcast flaming

Farmers are interested in producing popcorn under organic production systems and propane flaming could be a significant component of an integrated weed management program. The objective of this study was to collect baseline information on popcorn tolerance to broadcast flaming as influenced by propane dose and crop growth stage at the time of flaming. Field experiments were conducted at the Haskell Agricultural Laboratory of the University of Nebraska, Concord, NE in 2008 and 2009 using five propane doses (0, 13, 24, 44 and 85 kg ha(-1)) applied at the 2-leaf, 5-leaf and 7-leaf growth stages. Propane was applied using a custom-built research flamer driven at a constant speed of 6.4 km h(-1). Crop response to propane dose was described by log-logistic models on the basis of visual estimates of crop injury, yield components (plants m(-2), ears plant(-1), kernels cob(-1) and 100-kernel weight) and grain yield. Popcorn response to flaming was influenced by the crop growth stage and propane dose. Based on various parameters evaluated, popcorn flamed at the 5-leaf showed the highest tolerance while the 2-leaf was the most susceptible stage. The maximum yield reductions were 45%, 9% and 16% for the 2-leaf, 5-leaf and 7-leaf stages, respectively. In addition, propane doses that resulted in a 5% yield loss were 23 kg ha(-1) for the 2-leaf and 7-leaf and 30 kg ha(-1) for the 5-leaf stage. Flaming has a potential to be used effectively in organic popcorn production if properly used. (C) 2010 Elsevier Ltd. All rights reserved.

Resposta de plantas daninhas ao óleo fúsel aplicado em pós-emergência inicial e tardia

This work aimed to study weed response to fusel oil rate applied at early and late post-emergence. The following species were studied at late and early post-emergence: Ipomoea hederifolia, Ipomoea quamoclit, Euphorbia heterophylla, Digitaria spp., Cenchrus echinatus and Panicum maximum.. The experiment was arranged in a completely randomized design with six treatments and four replications. Fusel oil, an alcohol distillation byproduct, was applied at rates of 50, 125, 250, 375 and 500 L ha(-1), plus control without application. The plots were constituted by polyethylene containers with 3 L capacity, filled with topsoil land from a fallow area. Visual symptoms of intoxication were verified at 7 and 30 days after application (DAA) and dry weight at 30 DAA. The variables were submitted to variance analysis according to the design, adjusting the data to obtain the dose-response curve by polynomial regression. The studied species were susceptible only to the application of 500 L ha(-1) of fusel oil at early or late plant post-emergence. The species Digitaria spp. was susceptible and E. heterophylla tolerant to fusel oil applied at early post-emergence, while the other species were moderately tolerant. E. heterophylla was susceptible, Digitaria spp., C. echinatus and P. maximum moderately tolerant and I. hederifolia and I. quamoclit tolerant to fusel oil applied at late post-emergence.