Glutamate(NMDA) receptor NR1 subunit mRNA expression in Alzheimer's disease

We analyzed the expression profile of two NMDAR1 mRNA isoform subsets. NR1(0xx) and NR1(1xx), in discrete regions of human cerebral cortex. The subsets are characterized by the absence or presence of a 21-amino acid N-terminal cassette. Reverse transcription polymerase chain reaction for NR1 isoforms was performed on total RNA preparations from spared and susceptible regions from 10 pathologically confirmed Alzheimer's disease (AD) cases and 10 matched controls. Primers spanning the splice insert yielded two bands, 342 bp (NR1(0xx)) and 405 bp (NR1(1xx)), on agarose gel electrophoresis. The bands were visualized with ethidium and quantified by densitometry. NR1(1xx) transcript expression was calculated as a proportion of the NR1(1xx) + NR1(0xx) total. Values were significantly lower in AD cases than in controls in mid-cingulate cortex, p < 0.01, superior temporal cortex, p < 0.01 and hippocampus, p similar to 0.05. Cortical proportionate NR1(1xx) transcript expression was invariant over the range of ages acid areas of controls tested, at similar to 50%. This was also true for AD motor and occipital cortex. Proportionate NR1(1xx) expression in AD cingulate and temporal cortex was lower at younger ages and increased with age: this regression was significantly different from that in the homotropic areas of controls. Variations in NR1 N-terminal cassette expression may underlie the local vulnerability to excitotoxic damage of some areas in the AD brain. Alternatively, changes in NR1 mRNA expression may arise as a consequence of the AD disease process.

Population pharmacokinetics of lamotrigine

The present study estimated the population pharmacokinetics of lamotrigine in patients receiving oral lamotrigine therapy with drug concentration monitoring, and determined intersubject and intrasubject variability. A total of 129 patients were analyzed from two clinical sites. Of these, 124 patients provided spare data (198 concentration-time points); nine patients (four from a previous group plus five from the current group) provided rich data (431 points). The population analysis was conducted using P-PHARM (TM) (SIMED Scientific Software, Cedex, France), a nonlinear mixed-effect modeling program. A single exponential elimination model (first-order absorption) with heteroscedastic weighting was used. Apparent clearance (CL/F) and volume of distribution (V/F) were the pharmacokinetic parameters estimated. Covariate analysis was performed to determine which factors explained any of the variability associated with lamotrigine clearance. Population estimates of CL/F and V/F for lamotrigine generated in the final model were 2.14 +/- 0.81 L/h and 78.1 +/- 5.1 L/kg. Intersubject and intrasubject variability for clearance was 38% and 38%, respectively. The covariates of concomitant valproate and phenytoin therapy accounted for 42% of the intersubject variability of clearance. Age, gender, clinic site, and other concomitant antiepileptic drugs did not influence clearance. This study of the population pharmacokinetics of lamotrigine in patients using the drug clinically provides useful data and should lead to better dosage individualization for lamotrigine.

The urethral pressure profile and ultrasound imaging of the lower urinary tract

In a prospective study 105 patients with symptoms of stress incontinence underwent video-urodynamic testing, including resting urethral pressure profilometry and translabial ultrasound. The urethral pressure profile (UPP) included maximum urethral closure pressure (MUCP), functional length (FL) and area under the curve (AUC). Ultrasound parameters included urethral thickness, urethral rotation and bladder neck descent, as well as funneling/opening of the internal urethral meatus on Valsalva maneuver. Levator contraction strength was assessed measuring the cranioventral displacement of the internal meatus. Negative correlations between UPP data and age, parity and previous surgery were observed which were consistent with literature data. There was a positive correlation :between the urethral AP diameter on ultrasound and the MUCP, which agrees with reports showing reduced sphincter thickness or volume in stress-incontinent women. Hypermobility on ultrasound did not correlate with UPP data. However, a lower MUCP correlated with extensive opening of the bladder neck. Finally, there was a trend towards poorer pelvic floor function with lower MUCP measurements.

Barriers to effective cancer pain management: A survey of hospitalized cancer patients in Australia

The purpose of this study was to examine attitudinal barriers to effective pain management in a consecutively recruited cohort of 114 cancer patients from four Australian hospitals. When surveyed, 48% of this sample reported experiencing pain within the previous 24 hours. Of these, 56% reported this pain to be distressing, horrible or excruciating, with large proportions indicating that this pain had affected their movement, sleep and emotional well-being. Three factors were identified as potentially impacting on patients responses to pain-poor levels of patient knowledge about pain, low perceived control over pain, and a deficit in communication about pain. A trend for older patients to experience more severe pain was also identified. These older patients reported being more willing to tolerate pain and perceive less control over their pain. Suggestions are made for developing patient education programs and farther research using concepts drawn from broader social and behavioral models. J Pain Symptom Manage 2002:23:393-405. (C) U.S. Cancer Pain Relief Committee, 2002.

Fruit weight and shoot diameter relationship in early ripening peaches

Data were collected in early ripening peach [Prunus persica (L.) Batsch] varieties trained to a vase system to determine if a relationship exists between fruit weight and shoot diameter. The experiment was conducted with 3 varieties at Gainesville, FL with detailed pruning and with 3 other varieties at Atapulgus, GA with minimum pruning. All the varieties were similar in fruit development period (FDP) and fruit size. The largest shoot diameter was generally found in the upper canopy in all varieties. There was no correlation between shoot diameter and fruit weight for 'TropicBeauty', 'TropicSnow' and 'UF2000' at Gainesville under detailed pruning. There was a significant (p = 0.01) correlation for 'Flordacrest' in the lower (r = 0.53) canopy and for 'White Robin' in both the upper (r = 0.38) and lower (r = 0.40) canopy at Attapulgus, GA under minimal pruning. In these situations, large stems were associated with large fruit. 'Delta', grown at Attapulgus with minimal pruning, showed no correlation between shoot diameter and fruit weight, probably because it is male sterile and produced large fruit due to a reduced crop load.

Teacher Use of Learners' Native Language in the Foreign Language Classroom

This article reports on an exploratory study into the use of students' native language (NL) by teachers in the foreign language (FL) classroom. The project was undertaken by four teachers of beginner French at the University of Queensland. The teachers' aim was to investigate the use of NL in a context which actively promotes an immersion approach to FL teaching. The audio recordings of the teachers' speech were transcribed to provide data for estimating the amount of NL, and for analyzing the various instances of NL use. The study indicates that the activity type is a significant variable affecting NL amount. It also isolates two strategic uses of NL, translating FL words into NL, and contrasting NL and FL forms, both of which involve intrasentential code switching with NL words embedded in an FL sentence. The study suggests that these strategies may facilitate acquisition during immersion in FL, but experimental research is needed to test the hypothesis that translation and contrast facilitate learning of FL vocabulary and grammar.

Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs

Only a small proportion of the mouse genome is transcribed into mature messenger RNA transcripts. There is an international collaborative effort to identify all full-length mRNA transcripts from the mouse, and to ensure that each is represented in a physical collection of clones. Here we report the manual annotation of 60,770 full-length mouse complementary DNA sequences. These are clustered into 33,409 'transcriptional units', contributing 90.1% of a newly established mouse transcriptome database. Of these transcriptional units, 4,258 are new protein-coding and 11,665 are new non-coding messages, indicating that non-coding RNA is a major component of the transcriptome. 41% of all transcriptional units showed evidence of alternative splicing. In protein-coding transcripts, 79% of splice variations altered the protein product. Whole-transcriptome analyses resulted in the identification of 2,431 sense-antisense pairs. The present work, completely supported by physical clones, provides the most comprehensive survey of a mammalian transcriptome so far, and is a valuable resource for functional genomics.

Sistema de planejamento e execu????o or??ament??ria: SIPLAN

Apartir de janeiro de 1998, o Instituto Nacional de Metrologia, Normaliza????o e Qualidade Industrial (Inmetro) come??ou a desenvolver e a implementar o Sistema de Planejamento e Execu????o Or??ament??ria (SIPLAN), para o qual podemos destacar alguns pontos, com origem nas seguintes necessidades: constru????o de uma proposta or??ament??ria participativa, descentralizada, integrada ao planejamento estrat??gico, que possibilitasse o gerenciamento e o cumprimento dos prazos estabelecidos para o processo de sua elabora????o; gest??o or??ament??ria descentralizada, ??gil, transparente e confi??vel, disponibilizando a todos os n??veis hier??rquicos dados atualizados diariamente, propiciando um acompanhamento efetivo da execu????o dos projetos e processos da institui????o; planejamento das ??reas de apoio, permitindo uma gest??o corporativa de suas fun????es, de forma a exercerem um papel pr??-ativo nas demandas institucionais; racionaliza????o de formul??rios e pap??is no processo de elabora????o e acompanhamento do or??amento da institui????o; e ferramenta informatizada, dispon??vel em rede, que possibilitasse a qualquer gerente, que n??o fosse afeto ao jarg??o or??ament??rio, construir proposta consistente com suas necessidades, sem se tornar especialista em or??amento

Gest??o estrat??gica da Conab: avan??os e oportunidades de melhorias

Este estudo tem como objetivo realizar diagn??stico sobre o processo de gest??o da estrat??gia da Conab para identificar a integra????o do planejamento estrat??gico ?? sua execu????o operacional. Foi feita uma releitura da gest??o estrat??gica, passando pelos principais conceitos de planejamento estrat??gico at?? a descri????o do Sistema Gerencial, para a vincula????o da Estrat??gia ?? Opera????o em seus seis est??gios. Por meio de pesquisa explorat??ria e aplica????o de instrumento de coleta de dados junto aos gestores das ??reas fim e estrat??gica da Conab (Abastecimento e Pol??tica Agr??cola) foram obtidos resultados que apontaram a Companhia com um grau m??dio tr??s em maturidade estrat??gica, numa escala de zero a cinco. Diante deste resultado foi poss??vel considerar que, mesmo j?? dispondo de um planejamento estrat??gico com meios para operacionaliz??-lo, uma ??rea espec??fica e um mapa estrat??gico estabelecido, ainda h?? aus??ncia de sintonia dos seus gestores e colaboradores o que, de certa forma, acaba influenciando desfavoravelmente o processo de gest??o estrat??gica da empresa

Baixa de empresa web

Visando oferecer um servi??o inteligente, eficiente, ??gil e com maior qualidade ao contribuinte, a Diretoria de Arrecada????o do INSS disponibilizou, em conson??ncia com o Plano de A????o do Governo Eletr??nico e o Programa Nacional de Desburocratiza????o, o programa Baixa de Empresa Web, que viabiliza a emiss??o da certid??o de baixa para fins de registro ou arquivamento no ??rg??o pr??prio. Esse programa busca as informa????es contributivas da empresa e verifica a exist??ncia de restri????es ou impedimentos nos diversos bancos de dados do INSS, disponibilizando, em tempo real, o resultado do processamento. Os contribuintes podem acess??-lo pela Internet, no site da Previd??ncia Social (www.previdenciasocial.gov.br), e os servidores, pela Intranet

Curso de pol??ticas p??blicas e desenvolvimento econ??mico

O curso apresentou os seguintes temas: pol??ticas p??blicas macroecon??micas, setoriais e sociais como instrumento de estabiliza????o e desenvolvimento econ??mico; fundamentos econ??micos para as pol??ticas p??blicas; pol??tica macroecon??mica: pol??tica monet??ria e o combate ?? infla????o, pol??tica fiscal e desenvolvimento, pol??tica de com??rcio exterior e o c??mbio; pol??ticas setoriais: industrial, agr??cola, infraestrutura; a estrat??gia atual de desenvolvimento econ??mico e o papel das pol??ticas sociais

E se a nossa vida vira parque?

Trata-se de um estudo de caso sobre a cria????o de uma unidade de conserva????o ambiental em uma ??rea ocupada por comunidades tradicionais. No contexto do caso, h?? um conflito entre a perman??ncia da comunidade e a necessidade de prote????o ecol??gica para a ??rea, o que implica dilemas ??ticos e um contexto complexo para a tomada de decis??o em pol??ticas p??blicas. O caso traz como quest??es de fundo a autonomia relativa do Estado, o papel dos burocratas nas decis??es p??blicas e a import??ncia de n??o desvincular a prote????o ao meio ambiente da quest??o social. O caso ?? indicado para estudantes de administra????o p??blica, pol??ticas p??blicas, gest??o ambiental e ??reas afins

Os controles

O texto explora a quest??o dos controles administrativos na It??lia, tanto no que se refere aos controles internos quanto no que se refere aos controles externos. O autor aponta a debilidade dos controles internos, em particular no tocante aos controles de gest??o, e analisa o tipo mais difundido de controle externo na ordena????o italiana, os controles preventivos de legitimidade, que na sua opini??o deveriam ser substitu??dos pelos controles sucessivos sobre a atividade ou sobre a gest??o administrativas. O texto fala ainda da evolu????o das rela????es entre o Tribunal de Contas e o Parlamento, das tend??ncias recentes de transforma????o na ??rea de controle, incluindo aspectos relativos ?? reforma constitucional e ?? Uni??o Europ??ia, e das novidades legislativas surgidas em 1993 e 1994.

Organiza????es virtuais no setor p??blico: uma nova abordagem

?? crescente o envolvimento de organiza????es p??blicas e privadas com alian??as e parcerias. No novo cen??rio colaborativo, uma nova abordagem para o planejamento das organiza????es precisa ser desenvolvida, com especial aten????o para o setor p??blico, em cujo ambiente a escassez de recursos pode ser total ou parcialmente suprida pela cria????o ou incremento de redes entre organiza????es e sistemas. A nova abordagem para a coordena????o interorganizacional no ??mbito do planejamento governamental pode resultar em algum tipo de ???organiza????o virtual??? para o setor p??blico, ?? semelhan??a do que j?? ocorre no setor privado. O artigo destaca a import??ncia da reflex??o sobre as implica????es e possibilidades que os conceitos relativos a organiza????es virtuais podem trazer para a coordena????o interorganizacional no ??mbito do planejamento governamental, de forma a contribuir para incrementar a efetividade da a????o de governo, por meio de ambiente informacional cooperativo. Aspectos como cultura organizacional, poder e controle, fronteiras e estruturas organizacionais, confian??a e gest??o cooperativa da informa????o dever??o ser observados no contexto do planejamento governamental, caso se pretenda evoluir na aplica????o do conceito de organiza????es virtuais no setor p??blico

Clinton e a reinven????o do governo federal: o national performance review

O artigo analisa o National Performance Review (NPR), programa de reforma administrativa na gest??o do presidente Clinton, implementado em 1993, e que traduz o novo paradigma empreendedorialista proposto para o funcionamento da burocracia do governo federal americano. Na primeira parte s??o apresentados os princ??pios, objetivos e natureza da agenda, ressaltando-se as suas bases conceituais. Segue-se uma an??lise sobre a forma????o da agenda e a seq????ncia da sua implementa????o. A 3?? parte compreende uma vis??o da din??mica pol??tico-institucional, com destaque para as tens??es observadas entre o Legislativo e o Executivo, e o impacto destas nas mudan??as da agenda. Na ??ltima se????o, apresenta-se um painel emp??rico sobre alguns dos resultados em determinadas ag??ncias e departamentos do Governo, pretendendo-se oferecer contribui????es ao processo de reformas administrativas atualmente em curso no Brasil.