Advertisement (printed 1 page) advertising Charles Dwight, agent for The Western Bond Board of Kansas City

Advertisement (printed 1 page) advertising Charles Dwight, agent for The Western Bond Board of Kansas City, 1872, n.d.

Letter which was enclosed with the application, bond mortgage and abstract of the Jones and Mater accounts to Samuel Woodruff from Jarvis, Conklin and Co.

Letter which was enclosed with the application, bond mortgage and abstract of the Jones and Mater accounts to Samuel Woodruff from Jarvis, Conklin and Co., May 4, 1882.

Printed blank of Statement of Security Form naming the bond as Jones County Texas Bonds for the purpose of building a courthouse

Printed blank of Statement of Security Form naming the bond as Jones County Texas Bonds for the purpose of building a courthouse. It is made out to S.D. Woodruff and dated June 12, 1885. An envelope addressed to Mr. S.D. Woodruff is also included. The postmarks are Kansas, 1885 and St. Catharines, June 13, 1885.

The Ligand and Membrane-binding Behaviour of the Phosphatidylinositol Transfer Proteins (PITPa & PITPb)

Human Class I phosphatidylinositol transfer proteins (PITPs) exists in two forms: PITPα and PITPβ. PITPs are believed to be lipid transfer proteins based on their capacity to transfer either phosphatidylinositol (PI) or phosphatidylcholine (PC) between membrane compartments in vitro. In Drosophila, the PITP domain is found to be part of a multi-domain protein named retinal degeneration B (RdgBα). The PITP domain of RdgBα shares 40 % sequence identity with PITPα and has been shown to possess PI and PC binding and transfer activity. The detailed molecular mechanism of ligand transfer by the human PITPs and the Drosophila PITP domain remains to be fully established. Here, we investigated the membrane interactions of these proteins using dual polarization interferometry (DPI). DPI is a technique that measures protein binding affinity to a flat immobilized lipid bilayer. In addition, we also measured how quickly these proteins transfer their ligands to lipid vesicles using a fluorescence resonance energy transfer (FRET)-based assay. DPI investigations suggest that PITPβ had a two-fold higher affinity for membranes compared to PITPα. This was reflected by a four-fold faster ligand transfer rate for PITPβ in comparison to PITPα as determined by the FRET assay. Interestingly, DPI analysis also demonstrated that PI-bound human PITPs have lower membrane affinity compared to PC-bound PITPs. In addition, the FRET studies demonstrated the significance of membrane curvature in the ligand transfer rate of PITPs. The ligand transfer rate was higher when the accepting vesicles were highly curved. Furthermore, when the accepting vesicles contained phosphatidic acid (PA) which have smaller head groups, the transfer rate increased. In contrast, when the accepting vesicles contained phosphoinositides which have larger head groups, the transfer rate was diminished. However, PI, the favorite ligand of PITPs, or the presence of anionic lipids did not appear to influence the ligand transfer rate of PITPs. Both DPI and FRET examinations revealed that the PITP domain of RdgBα was able to bind to membranes. However, the RdgBα PITP domain appears to be a poor binder and transporter of PC.

Bond between Isaac Sterling of the Township of Salt fleet to William Dickson of Niagara and Thomas Clark of Queenston

Bond (1 page, printed) between Isaac Sterling of the Township of Salt fleet to William Dickson of Niagara and Thomas Clark of Queenston (regarding the Last Will and Testament of Robert Hamilton) for payment of 153 pounds, 12 shillings and 3 pence to be made to Dickson and Clark, May 21, 1819.

Bond between Archibald Johnston of the Township of Bertie to William Dickson of Niagara and Thomas Clark of Queenston

Bond (1 page, printed) between Archibald Johnston of the Township of Bertie to William Dickson of Niagara and Thomas Clark of Queenston (regarding the Last Will and Testament of Robert Hamilton) for payment of 75 pounds to be made to Dickson and Clark, June 7, 1821.

Bond between Daniel Cornell of the County of Oxford to William Dickson of Niagara and Thomas Clark of Stamford

Bond (1 page, printed) between Daniel Cornell of the County of Oxford to William Dickson of Niagara and Thomas Clark of Stamford (regarding the Last Will and Testament of Robert Hamilton) for payment of 103 pounds, 11 shillings and 1 penny to be made to Dickson and Clark, July 12, 1822.

Bond between John Hammell of the Township of Dumphries, Halton County to William Dickson of Niagara

Bond (1 page, printed) between John Hammell of the Township of Dumphries, Halton County to William Dickson of Niagara for 117 pounds, 10 shillings and 10 pence, Sept. 21, 1824.

Bond between Alexander Fraser of the Township of Dumphries to the Honourable William Dickson of Galt

Bond (1 page, printed) between Alexander Fraser of the Township of Dumphries to the Honourable William Dickson of Galt for 304 pounds, June 21, 1830.

Bond between Abram Quackenboss of Dumphries to the Honourable William Dickson of Galt

Bond (1 page, printed) between Abram Quackenboss of Dumphries to the Honourable William Dickson of Galt for 400 pounds, Oct. 27, 1836.

Marriage Bond between Harrison Narrel of Gainsborough and Anna Simmermon of Clinton

Marriage Bond between Harrison Narrel of Gainsborough and Anna Simmermon of Clinton signed by Andrew Snyder of Gainsborough and William Jacobs of Grimsby, Sept. 1, 1834

Marriage Bond between Henry Chambers of Ancaster and Sarah Smith of Ancaster

Marriage Bond between Henry Chambers of Ancaster and Sarah Smith of Ancaster signed by Charles Anderson of Grimsby and Robert F. Nelles of Grimsby, Dec. 27, 1836.

Marriage Bond between John Smith of Glanford in the Gore district and Jane Atkins of Ancaster

Marriage Bond between John Smith of Glanford in the Gore district and Jane Atkins of Ancaster signed by Edmund Smith of Ancaster and Jeremiah Smith of Glanford, Jan. 17, 1837.

Marriage Bond between Joseph Bridgeman of Grimsby and Calinda Ann Lounsberry of Grimsby

Marriage Bond between Joseph Bridgeman of Grimsby and Calinda Ann Lounsberry of Grimsby signed by Palmer Buckbee of Grimsby and Robert F. Nelles of Grimsby, July [?] 6, 1837.

Marriage Bond between Henry Stratton of Grimsby and Elizabeth Muir of Grimsby

Marriage Bond between Henry Stratton of Grimsby and Elizabeth Muir of Grimsby signed by William Crooks of Grimsby and Robert F. Nelles of Grimsby, June 7, 1838.