Effects of FSH on the expression of receptors for oocyte-secreted factors and members of the EGF-like family during in vitro maturation in cattle

FSH induces expansion of bovine cumulus-oocyte complexes (COCs) in cattle, which can be enhanced by oocyte-secreted factors (OSFs). In this study it was hypothesised that FSH stimulates COC expansion in part from direct stimulation of the epidermal growth factor (EGF)-like ligands amphiregulin (AREG), epiregulin (EREG) and betacellulin (BTC), but also in part through regulation of OSFs or their receptors in cumulus cells. Bovine COCs were cultured in defined medium with graded doses of FSH. In the absence of FSH, COCs did not expand. FSH caused cumulus expansion, and increased the abundance of AREG and EREG mRNA in a time- and dose-dependent manner, but decreased BTC mRNA levels. FSH had modest stimulatory effects on the levels of mRNA encoding the bone morphogenetic protein 15 (BMP15) receptor, BMPR1B, in cumulus cells, but did not alter mRNA expression of the growth and differentiation factor 9 (GDF9) receptor, TGFBR1. More interestingly, FSH dramatically stimulated levels of mRNA encoding two receptors for fibroblast growth factors (FGF), FGFR2C and FGFR3C, in cumulus cells. FSH also stimulated mRNA expression of FGFR1B, but not of FGFR2B in cumulus cells. Based on dose-response studies, FGFR3C was the receptor most sensitive to the influence of FSH. This study demonstrates that FSH stimulates the expression of EGF-like factors in bovine cumulus cells, and provides evidence that FSH differently regulates the expression of distinct receptors for OSFs in cumulus cells. © CSIRO 2013.

Cross-mating experiments detect reproductive compatibility between Triatoma sherlocki and other members of the Triatoma brasiliensis species complex

Phylogenetic approaches based on mitochondrial DNA variation (fragments of Cyt B and 16S ribosomal RNA) have revealed Triatoma sherlocki as the most recent species addition to the Triatoma brasiliensis species complex; a monophyletic group which includes T. brasiliensis, Triatoma melanica, and Triatoma juazeirensis. T. sherlocki is the most differentiated among all species of this complex: it is unable to fly, possesses longer legs than the other members, and has reddish tonality in some parts of its exochorion. We question whether these species are reproductively compatible because of this pronounced morphological differentiation, and therefore, we present a series of cross breeding experiments that test compatibility between T. sherlocki and other members of the T. brasiliensis complex. We extended our analyses to include crosses between T. sherlocki and Triatoma lenti, because the latter has been suggested as a possible member of this complex. T. sherlocki male. ×. T. lenti female pairs failed to produce hybrids. All other crosses of T. sherlocki and members of T. brasiliensis species complex, as well as backcrosses, produced viable offspring through the third generation. This study stresses the importance of searching for the features that may isolate members of the T. brasiliensis species complex. © 2013 Elsevier B.V.

La protección del medio marino como una modalidad de cooperación internacional: experiencias de apoyo entre el Plan de Acción del Pacífico Sudeste y la CEPAL = Protection of the marine environment as a modality of international co-operation: the Plan of Action of the South-East Pacific and ECLAC

Incluye Bibliografía

Informe de la Reunión de Expertos Gubernamentales de Países en Desarrollo Miembros de la CEPAL = Report of the Meeting of Government Experts from Developing Countries Members of ECLAC

Contiene conclusiones y recomendaciones de la reunión en la que se analizó el tema de la transformación productiva, equidad y sustentabilidad ambiental en América Latina y el Caribe, y se elaboró un proyecto que fue aprobado por el CEGAN, denominado Plataforma de Tlatelolco, el cual se incluye en este documento.

ECLAC's contribution to proposals for co-operation in the law of the sea and marine affairs among the states of the zone of peace and co-operation of the South Atlantic: analysis of some policy options for Latin American countries members of the zone

Includes bibliography

Reunión de Representantes de Países Miembros de la CEPAL ante las Naciones Unidas para Conocer el Informe de la Reunión del Grupo Especial de Trabajo = Meeting of Representatives of States Members of ECLAC to the United Nations to Hear the Report of the Meeting of the ad hoc Working Group = Réunion des Représentants des Pays Membres de la CEPALC auprès Nations Unies Visant à Prendre Connaissance du Rapport de la Réunion du Groupe de Travail Spécial

Rev.1 sólo en español

Report of Mission on Port Administration in the East Caribbean Countries: St. Vincent, St. Lucia and Dominica: 29 June to 28 September 1971

Includes bibliography