Maturidade sexual de touros da raça nelore, filhos de vacas superprecoces, precoces e normais, criados em condições extensivas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ultra-sonografia na avaliação andrológica de bovinos da raça Nelore

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação da biometria testicular, concentração plasmática de hormônios e minerais em bovinos Nelore variedade Mocha dos 12 aos 24 meses de idade

Pós-graduação em Medicina Veterinária - FMVZ

Efeito do cloreto de cádmio sobre as gônadas de girinos de rãs-touro (Lithobates catesbeianus)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência das épocas do ano na morfometria testicular e epididimária, características do sêmen e proteínas do sêmen em SDS-PAGE em zebus e taurinos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Comparison of Botu-bov and Tris as Freezing Extenders of Buffalo Sperm Recovered from Epididymal Cauda

Valuable genetic material can be preserved by the cryopreservation of epididymal sperm. This study evaluated the viability of pre-freezing and post-thawed sperm samples recovered from the epididymal cauda of buffaloes. Epididymides from eight Murrah buffaloes with 18 months of age were used. Semen samples were diluted in two different freezing extenders: Botu-bov (BB) and Tris (TRIS). Immediately after slaughter, both testicles from each animal were collected and transported at 4 degrees C for six hours interval. In laboratory, the removed epididymides were flushed to obtain sperm and the fractions were diluted in both freezing extenders (BB and TRIS). Semen doses were analyzed before and after frozen at -196 degrees C. BB and TRIS pre-freezing results were 38.54 +/- 22.33%(b) and 14.17 +/- 12.78%(a) for total motility (TM), 25.00 +/- 16.12(a) and 9.44 +/- 9.11(a) for progressive motility (PM), 7.21 +/- 0.98(a) and 5.09 +/- 2.65(a) for percentage of rapid cells (RAP), 91.08 +/- 12.53(b) and 63.33 +/- 31.47(a) for velocity of trajectory (VAP), 73.54 +/- 20.17(b) and 49.50 +/- 9.11(a) for linear progressive velocity (VSL), 172.21 +/- 24.55(a) and 116.94 +/- 59.48(a) for curvilinear velocity (VCL), respectively (P < 0.05). BB and TRIS post-thawing results were 42.25 +/- 21.50(b) and 17.62 +/- 19.46(a) for TM, 27.25 +/- 24.86(a) and 18.00 +/- 13.68(a) for PM, 7.35 +/- 0.98(a) and 6.26 +/- 1.13(a) for RAP, 91.42 +/- 16.86(a) and 75.96 +/- 13.17(a) for VAP, 67.96 +/- 12.13(a) and 60.04 +/- 10.42(a) for VSL, 177.54 +/- 23.53(b) and 141.29 +/- 24.97a for VCL, respectively (P < 0.05). The sperm recovered from the epididymal cauda, after 6 h storage of epididymides at 5 degrees C ensures sperm preservation demonstrating that the diluent Botu-bov had higher total motility both pre-and post-freezing when compared with TRIS. Additionally, the sperm frozen with the diluent Botu-bov showed higher values of VSL at post-thawing. These findings may reflect in improvement of conception rates.

Adding Motility Stimulants to Improve Freezing of Buffalo Sperm Recovered from Epididymal Cauda

The cryopreservation of epididymal sperm is important to preserve genetic material from valuable buffalo bulls. This study evaluated the viability of post-thawed sperm samples recovered from the epididymal cauda adding motility inductors. For that, were used epididymides from eight Murrah buffaloes with 18 months of age. Semen samples were submitted to three different conditions: (CT - control) without adding medium, (SPERM) adding Sperm Talp medium, and (FERT) adding Fert Talp medium. Immediately after slaughter, both testicles from each animal were collected and transported at 4 degrees C at maximum six hours interval. In laboratory, the removed epididymides was flushed to obtain sperm and diluted in the freezing extender. Each buffalo sperm were divided and fractions were submitted to all conditions (CT, SPERM and FERT). Semen doses were frozen at -196 degrees C. CT, SPERM and FERT post-thawing results were 13.63 +/- 8.91, 38.77 +/- 8.91 and 42.83 +/- 8.91 for total motility, 7.30 +/- 8.74, 24.87 +/- 8.74 and 29.70 +/- 8.74 for progressive motility, 6.04 +/- 0.92, 6.74 +/- 0.92 and 6.93 +/- 0.92 for percentage of rapid cells (P < 0.05). In conclusion, diluted semen supplementation with Sperm or Fert talp increases the motility of cauda epididymal sperm of buffalo bulls.

Morphological aspects of the testes of 18 species of terrestrial of Heteroptera from Northwestern Sao Paulo (Brazil)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A persistent organic pollutant related with unusual high frequency of hermaphroditism in the neotropical anuran Physalaemus cuvieri Fitzinger, 1826

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Precocidade sexual em bovinos Nelore avaliada por ultrassonografia testicular

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mecanismos da infertilidade causada por Gossipol em ratos e efeito protetor da vitamina E

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Pesquisa de Toxoplasma gondii (Nicolle & Manceaux, 1909) em sêmen, testículos e epidídimos de gatos (Felis catus) primoinfectados

Pós-graduação em Medicina Veterinária - FCAV

Ultrassonografia de glândulas genitais acessórias em carneiros da raça Dorper: caracterização e correlações reprodutivas

Pós-graduação em Medicina Veterinária - FCAV

Avaliação da técnica de coloração AgNOR em testículos de ovinos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência da contaminação por Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) na espermatogênese de Triatomíneos (Hemiptera: Reduviidae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)