Evaluation of Bt and Non-Bt Corn with and without Soil Insecticides for Control of Corn Rootworm

The purpose of this study was to evaluate the effectiveness of Bt corn and soil insecticides, either alone or in combination, for the control of corn rootworm. Evaluation of Bt hybrids included SmartStax, SmartStax with refuge in a bag, and Herculex XTRA. Soil insecticides evaluated were SmartChoice-SB, Counter-SB, Aztec, and Force.

Evaluation of Foliar Fungicides and Insecticides on Soybean

Use of foliar fungicides and insecticides are an effective strategy for managing foliar diseases on soybean. There are many different fungicides and insecticides available for use currently in Iowa. Iowa State University personnel assessed the success of fungicides and insecticides across Iowa. This study was conducted at six locations: Sutherland (NW), Kanawha (NC), Nashua (NE), Ames (central), Crawfordsville (SE), and Lewis (SW) research farms (Figure 1).

Evaluation of Foliar Fungicides and Insecticides on Soybeans

Use of foliar fungicides and insecticides are an effective strategy for managing foliar diseases of soybean. There are many different fungicides and insecticides available for use currently in Iowa. Iowa State University personnel assessed the success of fungicides and insecticides across Iowa. This study was conducted at six locations: Sutherland (NW), Kanawha (NC), Nashua (NE), Ames (central), Crawfordsville (SE), and Lewis (SW) research farms (Figure 1).

Evaluation of Bt and Non-Bt Corn with and without Soil Insecticides for Control of Corn Rootworm

The purpose of this study was to evaluate the effectiveness of Bt corn and soil insecticides, either alone or in combination, for the control of corn rootworm. Evaluation of Bt hybrids included SmartStax, SmartStax with a blended refuge (refuge in the bag), and Herculex XTRA. Soil insecticides evaluated were SmartChoice-SB, Counter-SB, Aztec, and Force.

Evaluation of Foliar Fungicides and Insecticides on Soybeans

Use of foliar fungicides and insecticides are an effective strategy for managing foliar diseases of soybean. There are many different fungicides and insecticides available for use currently in Iowa. Iowa State University personnel assessed the success of fungicides and insecticides across Iowa. This study was conducted at six locations: Sutherland (NW), Kanawha (NC), Nashua (NE), Ames (central), Crawfordsville (SE), and Lewis (SW) research farms (Figure 1).

Evaluation of Foliar Fungicides and Insecticides on Soybeans

Use of foliar fungicides and insecticides are an effective strategy for managing foliar diseases of soybean. There are many different fungicides and insecticides available for use currently in Iowa. Iowa State University personnel assessed the success of fungicides and insecticides across Iowa. This study was conducted at six locations: Sutherland (NW), Kanawha (NC), Nashua (NE), Ames (central), Crawfordsville (SE), and Lewis (SW) research farms (Figure 1).

Phyto- and zooplankton of the southeastern Barents Sea in April 2000

Spring bloom of cold-water centric and pennate diatoms was observed in two different areas of the southeastern Barents Sea in April 2000: ice-free waters off the Kolguev Island northern shelf and the eastern Pechora Sea near the Karskie Vorota (Kara Gate) Straight in polynyas and ice-free patches in one-year-old ice. Maximal values of phytoplankton abundance and biomass were found at the ice edge. The bloom was localized in shallow water areas with depths less than 50 m in mixing zones of waters of different origin: warm Atlantic, cold coastal, and Arctic (Litke current) waters. Ice melting was among factors inducing the phytoplankton bloom. Each area had a specific phytocoenosis, whose structure was determined by water origin and ice conditions. In the western Kara Sea, under a solid (up to 30 cm thick) ice cover (i.e., under conditions of a hydrological winter), a spring phytoplankton succession was observed from its initial stage. In areas located close to the ice-cover edge, simultaneously with the mass phytoplankton bloom, the early spring zoocoenosis development manifested itself in mass spawning of euphausiids and mass appearance of Cirripedia nauplii and bottom polychaete larvae.

Selectivity of diacylhydrazine insecticides to the predatory bug Orius laevigatus: In vivo and modeling/docking experiments

BACKGROUND: Knowledge of pesticide selectivity to natural enemies is necessary for a successful implementation of biological and chemical control methods in integrated pest management (IPM) programs. Diacylhydrazine (DAH)-based ecdysone agonists also known as molting-accelerating compounds (MACs) are considered a selective group of insecticides, and their compatibility with predatory Heteroptera, which are used as biological control agents, is known. However, their molecular mode of action has not been explored in beneficial insects such as Orius laevigatus (Fieber) (Hemiptera: Anthocoridae). RESULTS: In this project in vivo toxicity assays demonstrated that the DAH-based RH-5849, tebufenozide and methoxyfenozide have no toxic effect against O. laevigatus. The ligand-binding domain (LBD) of the ecdysone receptor (EcR) of O. laevigatus was sequenced and a homology protein model was constructed which confirmed a cavity structure with 12 ?-helixes, harboring the natural insect molting hormone 20-hydroxyecdysone. However, docking studies showed that a steric clash occurred for the DAH-based insecticides due to a restricted extent of the ligand-binding cavity of the EcR of O. laevigatus. CONCLUSIONS: The insect toxicity assays demonstrated that MACs are selective for O. laevigatus. The modeling/docking experiments are indications that these pesticides do not bind with the LBD-EcR of O. laevigatus and support that they show no biological effects in the predatory bug. These data help in explaining the compatible use of MACs together with predatory bugs in IPM programs. Keywords: Orius laevigatus, selectivity, diacylhydrazine insecticides, ecdysone receptor, homology modelling, docking studies.

Insect growth regulators as potential insecticides to control olive fruit fly (Bactrocera oleae Rossi): insect toxicity bioassays and molecular docking approach

Olive fruit fly, Bactrocera oleae (Rossi), is a key pest in olive orchards, causing serious economic damage. To date, the pest has already developed resistance to the insecticides commonly applied to control it. Thus, in searching for new products for an accurate resistance management programme, targeting the ecdysone receptor (EcR)might provide alternative compounds for use in such programmes. RESULTS: Residual contact and oral exposure in the laboratory of B. oleae adults to the dibenzoylhydrazine-based compounds methoxyfenozide, tebufenozide and RH-5849 showed different results. Methoxyfenozide and tebufenozide did not provoke anynegative effectsontheadults,but RH-5849 killed98-100%of the treated insects15 days after treatment. Theligand-binding domain (LBD) of the EcR of B. oleae (BoEcR-LBD) was sequenced, and a homology protein model was constructed. Owing to a restricted extent of the ligand-binding cavity of the BoEcR-LBD, docking experiments with the three tested insecticides showed a severe steric clash in the case of methoxyfenozide and tebufenozide, while this was not the case with RH-5849. CONCLUSION: IGR molecules similar to the RH-5849 molecule, and different from methoxyfenozide and tebufenozide, might have potential in controlling this pest.

Toxicity and sublethal effects of six insecticides to last instar larvae and adults of the biocontrol agents Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) and Adalia bipunctata (L.) (Coleoptera: Coccinellidae)

To further develop Integrated Pest Management (IPM) strategies against crop pests, it is important to evaluate the effects of insecticides on biological control agents. Therefore, we tested the toxicity and sublethal effects (fecundity and fertility) of flonicamid, flubendiamide, metaflumizone, spirotetramat, sulfoxaflor and deltamethrin on the natural enemies Chrysoperla carnea and Adalia bipunctata. The side effects of the active ingredients of the insecticides were evaluated with residual contact tests for the larvae and adults of these predators in the laboratory. Flonicamid, flubendiamide, metaflumizone and spirotetramat were innocuous to last instar larvae and adults of C. carnea and A. bipunctata. Sulfoxaflor was slightly toxic to adults of C. carnea and was highly toxic to the L4 larvae of A. bipunctata. For A. bipunctata, sulfoxaflor and deltamethrin were the most damaging compounds with a cumulative larval mortality of 100%. Deltamethrin was also the most toxic compound to larvae and adults of C. carnea. In accordance with the results obtained, the compounds flonicamid, flubendiamide, metaflumizone and spirotetramat might be incorporated into IPM programs in combination with these natural enemies for the control of particular greenhouse pests. Nevertheless, the use of sulfoxaflor and deltamethrin in IPM strategies should be taken into consideration when releasing either of these biological control agents, due to the toxic behavior observed under laboratory conditions. The need for developing sustainable approaches to combine the use of these insecticides and natural enemies within an IPM framework is discussed.

Ecotoxicity of insecticides of frequent use in tomatoes on Trichogramma achaeae (Hymenoptera: Trichogrammatidae)

Since Tuta absoluta(Meyrick) (Lepidoptera: Gelechiidae) was detected in 2006 as a new pest in tomato crops in Spain, several natural enemies have been reported tocontrol this pest. In biological control programs, the native parasitoid Trichogramma achaeae Nagaraja&Nagarkatti (Hymenoptera: Trichogrammatidae) is used against T.absoluta. However, the most common control practice is based on use of pesticides,and in the frame of Integrated Pest Management (IPM) programs, the knowledge on the activity of insecticides towards beneficial insects is needed for its joint use. In thiswork, we evaluated lethal and sublethal effects of insecticides commonly applied on tomato crops on adults of T. achaeae. Pesticides were sprayed on tomato plants or T. Absoluta eggs till run off at their maximum field recommended concentration. Mortality was scored after 24, 48 and 72 hours, as well as beneficial capacity and percentage of emergence.

Multiresidue Analysis of Insecticides and Other Selected Environmental Contaminants in Poultry Manure by Gas Chromatography/Mass Spectrometry

An analytical method was developed for the simultaneous determination in poultry manure of 41 organic contaminants belonging to different chemical classes: pesticides, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and polybrominated diphenyl ethers. Poultry manure was extracted with a modified QuEChERS method, and the extracts were analyzed by isotope dilution GC/MS. Recovery of these contaminants from samples spiked at levels ranging from 25 to 100 ng/g was satisfactory for all the compounds. The developed procedure provided LODs from 0.8 to 9.6 ng/g. The analysis of poultry manure samples collected on different farms confirmed the presence of some of the studied contaminants. Pyrethroids and polycyclic aromatic hydrocarbons were the main contaminants detected.

Alternatives for reducing insecticides on cotton and corn : economic and environmental impact /

"Grant no. R802518-02."