La nueva condición juvenil : reflexiones sobre los sentidos y prácticas que configuran las trayectorias laborales de jóvenes pobres

En un contexto de desestructuración del mundo del trabajo, persisten viejos y nuevos problemas en la población juvenil, que se presenta como uno de los grupos más vulnerables. En efecto, las nuevas generaciones experimentan la crisis de las distintas instituciones y, en consecuencia, de aquellas pautas de integración consideradas hegemónicas para un momento histórico-social determinado. Los cambios ocurridos durante las últimas décadas, han implicado profundas transformaciones en la condición juvenil, que encuentra una de sus manifestaciones más relevantes en las trayectorias laborales que delinean los jóvenes. En este marco, el presente artículo investiga el modo en que se articulan sentidos y prácticas en la constitución de las trayectorias laborales de jóvenes residentes de un barrio de la periferia sudoeste de la ciudad de La Plata, Provincia de Buenos Aires. Se propone analizar los sentidos que otorgan al trabajo los jóvenes a lo largo de su vida; buscando aprehender al mismo tiempo las lógicas que orientan su accionar en este ámbito, que da cuenta de una compleja trama entre la esfera laboral y otros mundos de la vida. Desde una perspectiva cualitativa, se realizan entrevistas en profundidad buscando aprehender el conjunto de procesos, prácticas e imaginarios juveniles a partir de una caracterización objetiva y subjetiva del itinerario laboral. En esta línea, se presentan algunos puntos que surgen del análisis y que aparecen como temas de reflexión relevantes de cara a generar una discusión más profunda que aporte a la comprensión del fenómeno juvenil.

La nueva condición juvenil : Reflexiones sobre los sentidos y prácticas que configuran las trayectorias laborales de jóvenes pobres

En un contexto de desestructuración del mundo del trabajo, persisten viejos y nuevos problemas en la población juvenil, que se presenta como uno de los grupos más vulnerables. En efecto, las nuevas generaciones experimentan la crisis de las distintas instituciones y, en consecuencia, de aquellas pautas de integración consideradas hegemónicas para un momento histórico-social determinado. Los cambios ocurridos durante las últimas décadas, han implicado profundas transformaciones en la condición juvenil, que encuentra una de sus manifestaciones más relevantes en las trayectorias laborales que delinean los jóvenes. En este marco, el presente artículo investiga el modo en que se articulan sentidos y prácticas en la constitución de las trayectorias laborales de jóvenes residentes de un barrio de la periferia sudoeste de la ciudad de La Plata, Provincia de Buenos Aires. Se propone analizar los sentidos que otorgan al trabajo los jóvenes a lo largo de su vida; buscando aprehender al mismo tiempo las lógicas que orientan su accionar en este ámbito, que da cuenta de una compleja trama entre la esfera laboral y otros mundos de la vida. Desde una perspectiva cualitativa, se realizan entrevistas en profundidad buscando aprehender el conjunto de procesos, prácticas e imaginarios juveniles a partir de una caracterización objetiva y subjetiva del itinerario laboral. En esta línea, se presentan algunos puntos que surgen del análisis y que aparecen como temas de reflexión relevantes de cara a generar una discusión más profunda que aporte a la comprensión del fenómeno juvenil.

La nueva condición juvenil : Reflexiones sobre los sentidos y prácticas que configuran las trayectorias laborales de jóvenes pobres

En un contexto de desestructuración del mundo del trabajo, persisten viejos y nuevos problemas en la población juvenil, que se presenta como uno de los grupos más vulnerables. En efecto, las nuevas generaciones experimentan la crisis de las distintas instituciones y, en consecuencia, de aquellas pautas de integración consideradas hegemónicas para un momento histórico-social determinado. Los cambios ocurridos durante las últimas décadas, han implicado profundas transformaciones en la condición juvenil, que encuentra una de sus manifestaciones más relevantes en las trayectorias laborales que delinean los jóvenes. En este marco, el presente artículo investiga el modo en que se articulan sentidos y prácticas en la constitución de las trayectorias laborales de jóvenes residentes de un barrio de la periferia sudoeste de la ciudad de La Plata, Provincia de Buenos Aires. Se propone analizar los sentidos que otorgan al trabajo los jóvenes a lo largo de su vida; buscando aprehender al mismo tiempo las lógicas que orientan su accionar en este ámbito, que da cuenta de una compleja trama entre la esfera laboral y otros mundos de la vida. Desde una perspectiva cualitativa, se realizan entrevistas en profundidad buscando aprehender el conjunto de procesos, prácticas e imaginarios juveniles a partir de una caracterización objetiva y subjetiva del itinerario laboral. En esta línea, se presentan algunos puntos que surgen del análisis y que aparecen como temas de reflexión relevantes de cara a generar una discusión más profunda que aporte a la comprensión del fenómeno juvenil.

Executive Managers in Peru's Family Businesses

As in many other developing countries, family businesses are major players in the Peruvian economy. Despite their growth into large-scale groups spanning a wide range of businesses, the owner families still have strong control over their ownership and management. However, Peru's liberal economic reforms in the 1990s brought intense competition into the national market. Not only have these family businesses been forced to compete against large-scale foreign capital that entered the national market through the privatization of state enterprises, but also against cheap goods imported from foreign countries. In order to compete, family businesses have had to move beyond the limited human resources available within the family. The advancement within owner families of new generations with better education and training together with the promotion to top managerial positions of professional salaried managers from outside the family are some of the measures owner families are taking to overcome their human resource limitations.

Helping to Close the Generational Gap in Nuclear: The Seminars and Conferences of Spanish Young Generation in Nuclear (Jóvenes Nucleares)

As in each country of Europe with nuclear power, there is a clear gap between those generation that have built the power plants in the eighties and the new generations with less than ten years of experience in the nuclear field. From its creation, Spanish Young Generation in Nuclear (Jóvenes Nucleares) has as an important scope to help transferring the knowledge between those generations in the way that it can be possible. Some years ago, JJNN have started organizing seminars periodically trying to cover as many areas as possible in the nuclear engineering field, and some of them outside the industry but related with it.

Proceso de replanteo de un nodo urbano

El continuo desarrollo que está teniendo el mundo de las telecomunicaciones móviles hace que la red móvil esté sufriendo progresivos cambios para adaptarse a las nuevas tecnologías móviles que ofrecen un mejor servicio. El cambio en la red móvil no solo se produce por el desarrollo de las nuevas generaciones móviles. La red móvil se adapta también a la demanda de usuarios la cual no deja de incrementar a lo largo de los últimos años. Por tanto, los operadores tienen que ampliar su red instalando nodos que tengan las nuevas tecnologías y también las anteriores. Aparte de crear nuevos nodos también tienen que modificar sus nodos antiguos y convertirlos en nodos que soporten mayor número de usuarios. Hoy en día, en España, se están instalando nuevos nodos con 2G, 3G y 4G y además se están realizando ampliaciones de portadora para 3G. Este proyecto se divide en cuatro partes, la primera de ella se centra en explicar el proceso a seguir para la instalación de un nuevo nodo urbano. Este proceso es muy parecido para instalar un nodo con una tecnología u otra, en el caso del proyecto se explicarán los pasos a seguir para la instalación de un nodo con 2G y 3G. Posteriormente se explicará cómo se realizan las medidas para corroborar el correcto funcionamiento de un nodo rural y se compararán a las medidas de zona urbana mediante capturas de un nodo específico. En la penúltima parte del proyecto se estudia la cobertura en interiores y las diversas soluciones que se toman normalmente para mejorar dicha cobertura en edificios, almacenes y centros comerciales. Por último aparecen las conclusiones del proyecto y los trabajos futuros en donde se realiza una visión de posibles estudios relacionados con este proyecto y una visión de cómo puede quedar formada la red en unos años. ABSTRACT. Due to the continuous development of mobile telecommunications the mobile networks have undergone rapid changes to adapt to new mobile technologies that offer a better service. The mobile network change hasn´t only occurred because of the development of new generations of mobile radio-communications. The mobile network adapts itself to user demand, which has been growing over the last few years faster than expected. Therefore, mobile operators have to enlarge its network by installing nodes that share the old and new technologies. Apart from creating new nodes, the operators have to modify the old ones and turn them into nodes that support an increasing number of users. Nowadays, in Spain new nodes with 2G 3G and 4G are being installed, and carrier extensions for 3G are being made as well. This project is divided into four parts. The first chapter focuses on explaining the process that should be followed to install a new urban node. This process is similar to install a node with any of the technologies available. In the case of this project, the steps to follow in setting up a wireless node with 2G and 3G will be detailed. Afterwards, in the second chapter the document continues explaining the measurements that should be carried out to ensure proper performance of a rural node. Then, those measurements will be compared with the ones of an urban node. In the third part of the project it is explained how coverage indoor studies are performed, and the different solutions that are usually proposed to improve coverage in buildings, stores and shopping centers. The last chapter explains the conclusions that have been reached and future works. It is provided a widespread view of possible studies related to this project and how the mobile will improve in the following years.

A Porposal: "Agro-ERASMUS"

The rural population is getting smaller as percentage of the total population in the countries. There is a constant depopulation of rural areas to urban areas. The most extreme data are in countries like USA, where the rural population is 1.5%, from which 1% of that amount is part time and only 0.5% full time. On the other side, we have countries with more than 50% rural population. Related to training, cultural development, business and specific weight in society, rural residents have no significance in their societies. As they are few, and separated across the territory they have no influence on their societies. Comparing the USA farmer with one from the EU, we see that the American one is a businessperson and the European one, in most cases is a farm worker. To reduce this gap between these different farmers, we believe that we must train the new generations of children belonging to farming Europe. They must have a common language, English; they must know other countries culture and farming systems, live and network with other young Europeans colleagues,future young farmers. It is what we have coined as AGRO-ERASMUS. A project to be placed within the EU Common Agriculture Policies. The project must be designed before its implementation. Even some previous experience should make better viability. It should make use of a network of agricultural universities in several European countries. Each university would build a "farm school" where young people would learn "English?, and visit and work in small agricultural practices with a correct use of the time. One important subject dealing with should be agribusiness. The procedure based on the ?Farm School? (F-S) experience, should start with young people from 13 years up to 18 years. Their attendance, every summer, to the F-S should be rotated between different countries besides their own. The first and second year, with young people 13/14 years old, the Farm School would last less than three weeks in an English speaking country (Ireland, UK or someone else). They should live with a local family the time they stay outside of the Farm School (F-S). This two years period must be devoted to learn and become familiar with the English language and cultural differences. The rest of the four years left, the Farm Schools will have longer duration and be placed in other countries from the network. The living way would be in multinational teams of young people where the only spoken language would be English. After six years of summer oexistence speaking English and learning new competences and skills with colleagues from other countries, we would have a great team of young and future European farmers, able to travel free and confident through the whole Europe and ready to be engaged in productive, commercial and research activities. These new young farmers may revive European agriculture and would not look any more like rural habitants, but international business-farmers, professionally speaking. In a brief survey among the assistants to the Fifth International Academic Conference titled "Alternative Income Sources in Small Agricultural Holdings of the European Union" held in Krakow (PL) in June 2015, participants from universities and countries like Poland, Hungary, Rep. Czech, Portugal, Romania, etc., expressed the necessity of addressing this problem in a new and bold way.

50 Years Ago We Marched: What began on the grounds of Lincoln University, ended on the steps of the Alabama State Capitol

LINCOLN UNIVERSITY - On March 25, 1965, a bus loaded with Lincoln University students and staff arrived in Montgomery, Ala. to join the Selma march for racial and voting equality. Although the Civil Rights Act of 1964 was in force, African-Americans continued to feel the effects of segregation. The 1960s was a decade of social unrest and change. In the Deep South, specifically Alabama, racial segregation was a cultural norm resistant to change. Governor George Wallace never concealed his personal viewpoints and political stance of the white majority, declaring “Segregation now, segregation tomorrow, segregation forever.” The march was aimed at obtaining African-Americans their constitutionally protected right to vote. However, Alabama’s deep-rooted culture of racial bias began to be challenged by a shift in American attitudes towards equality. Both black and whites wanted to end discrimination by using passive resistance, a movement utilized by Dr. Martin Luther King Jr. That passive resistance was often met with violence, sometimes at the hands of law enforcement and local citizens. The Selma to Montgomery march was a result of a protest for voting equality. The Student Nonviolent Coordinating Committee (SNCC) and the Southern Christian Leadership Counsel (SCLC) among other students marched along the streets to bring awareness to the voter registration campaign, which was organized to end discrimination in voting based on race. Violent acts of police officers and others were some of the everyday challenges protesters were facing. Forty-one participants from Lincoln University arrived in Montgomery to take part in the 1965 march for equality. Students from Lincoln University’s Journalism 383 class spent part of their 2015 spring semester researching the historical event. Here are their stories: Peter Kellogg “We’ve been watching the television, reading about it in the newspapers,” said Peter Kellogg during a February 2015 telephone interview. “Everyone knew the civil rights movement was going on, and it was important that we give him (Robert Newton) some assistance … and Newton said we needed to get involve and do something,” Kellogg, a lecturer in the 1960s at Lincoln University, discussed how the bus trip originated. “That’s why the bus happened,” Kellogg said. “Because of what he (Newton) did - that’s why Lincoln students went and participated.” “People were excited and the people along the sidewalk were supportive,” Kellogg said. However, the mood flipped from excited to scared and feeling intimidated. “It seems though every office building there was a guy in a blue uniform with binoculars standing in the crowd with troops and police. And if looks could kill me, we could have all been dead.” He says the hatred and intimidation was intense. Kellogg, being white, was an immediate target among many white people. He didn’t realize how dangerous the event in Alabama was until he and the others in the bus heard about the death of Viola Liuzzo. The married mother of five from Detroit was shot and killed by members of the Ku Klux Klan while shuttling activists to the Montgomery airport. “We found out about her death on the ride back,” Kellogg recalled. “Because it was a loss of life, and it shows the violence … we could have been exposed to that danger!” After returning to LU, Kellogg’s outlook on life took a dramatic turn. Kellogg noted King’s belief that a person should be willing to die for important causes. “The idea is that life is about something larger and more important than your own immediate gratification, and career success or personal achievements,” Kellogg said. “The civil rights movement … it made me, it made my life more significant because it was about something important.” The civil rights movement influenced Kellogg to change his career path and to become a black history lecturer. Until this day, he has no regrets and believes that his choices made him as a better individual. The bus ride to Alabama, he says, began with the actions of just one student. Robert Newton Robert Newton was the initiator, recruiter and leader of the Lincoln University movement to join Dr. Martin Luther King’s march in Selma. “In the 60s much of the civil rights activists came out of college,” said Newton during a recent phone interview. Many of the events that involved segregation compelled college students to fight for equality. “We had selected boycotts of merchants, when blacks were not allowed to try on clothes,” Newton said. “You could buy clothes at department stores, but no blacks could work at the department stores as sales people. If you bought clothes there you couldn’t try them on, you had to buy them first and take them home and try them on.” Newton said the students risked their lives to be a part of history and influence change. He not only recognized the historic event of his fellow Lincolnites, but also recognized other college students and historical black colleges and universities who played a vital role in history. “You had the S.N.C.C organization, in terms of voting rights and other things, including a lot of participation and working off the bureau,” Newton said. Other schools and places such as UNT, Greenville and Howard University and other historically black schools had groups that came out as leaders. Newton believes that much has changed from 50 years ago. “I think we’ve certainly come a long way from what I’ve seen from the standpoint of growing up outside of Birmingham, Alabama,” Newton said. He believes that college campuses today are more organized in their approach to social causes. “The campus appears to be some more integrated amongst students in terms of organizations and friendships.” Barbara Flint Dr. Barbara Flint grew up in the southern part of Arkansas and came to Lincoln University in 1961. She describes her experience at Lincoln as “being at Lincoln when the world was changing.“ She was an active member of Lincoln’s History Club, which focused on current events and issues and influenced her decision to join the Selma march. “The first idea was to raise some money and then we started talking about ‘why can’t we go?’ I very much wanted to be a living witness in history.” Reflecting on the march and journey to Montgomery, Flint describes it as being filled with tension. “We were very conscious of the fact that once we got on the road past Tennessee we didn’t know what was going to happen,” said Flint during a February 2015 phone interview. “Many of the students had not been beyond Missouri, so they didn’t have that sense of what happens in the South. Having lived there you knew the balance as well as what is likely to happen and what is not likely to happen. As my father use to say, ‘you have to know how to stay on that line of balance.’” Upon arriving in Alabama she remembers the feeling of excitement and relief from everyone on the bus. “We were tired and very happy to be there and we were trying to figure out where we were going to join and get into the march,” Flint said. “There were so many people coming in and then we were also trying to stay together; that was one of the things that really stuck out for me, not just for us but the people who were coming in. You didn’t want to lose sight of the people you came with.” Flint says she was keenly aware of her surroundings. For her, it was more than just marching forward. “I can still hear those helicopters now,” Flint recalled. “Every time the helicopters would come over the sound would make people jump and look up - I think that demonstrated the extent of the tenseness that was there at the time because the helicopters kept coming over every few minutes.” She said that the marchers sang “we are not afraid,” but that fear remained with every step. “Just having been there and being a witness and marching you realize that I’m one of those drops that’s going to make up this flood and with this flood things will move,” said Flint. As a student at Lincoln in 1965, Flint says the Selma experience undoubtedly changed her life. “You can’t expect to do exactly what you came to Lincoln to do,” Flint says. “That march - along with all the other marchers and the action that was taking place - directly changed the paths that I and many other people at Lincoln would take.” She says current students and new generations need to reflect on their personal role in society. “Decide what needs to be done and ask yourself ‘how can I best contribute to it?’” Flint said. She notes technology and social media can be used to reach audiences in ways unavailable to her generation in 1965. “So you don’t always have to wait for someone else to step out there and say ‘let’s march,’ you can express your vision and your views and you have the means to do so (so) others can follow you. Jaci Newsom Jaci Newsom came to Lincoln in 1965 from Atlanta. She came to Lincoln to major in sociology and being in Jefferson City was largely different from what she had grown up with. “To be able to come into a restaurant, sit down and be served a nice meal was eye-opening to me,” said Newsom during a recent interview. She eventually became accustomed to the relaxed attitude of Missouri and was shocked by the situation she encountered on an out-of-town trip. “I took a bus trip from Atlanta to Pensacola and I encountered the worse racism that I have ever seen. I was at bus stop, I went in to be served and they would not serve me. There was a policeman sitting there at the table and he told me that privately owned places could select not to serve you.” Newsom describes her experience of marching in Montgomery as being one with a purpose. “We felt as though we achieved something - we felt a sense of unity,” Newsom said. “We were very excited (because) we were going to hear from Martin Luther King. To actually be in the presence of him and the other civil rights workers there was just such enthusiasm and excitement yet there was also some apprehension of what we might encounter.” Many of the marchers showed their inspiration and determination while pressing forward towards the grounds of the Alabama Capitol building. Newsom recalled that the marchers were singing the lyrics “ain’t gonna let nobody turn me around” and “we shall overcome.” “ I started seeing people just like me,” Newsom said. “I don’t recall any of the scowling, the hitting, the things I would see on TV later. I just saw a sea of humanity marching towards the Capitol. I don’t remember what Martin Luther King said but it was always the same message: keep the faith; we’re going to get where we’re going and let us remember what our purpose is.” Newsom offers advice on what individuals can do to make their society a more productive and peaceful place. “We have come a long way and we have ways to change things that we did not have before,” Newsom said. “You need to work in positive ways to change.” Referencing the recent unrest in Ferguson, Mo., she believes that people become destructive as a way to show and vent anger. Her generation, she says, was raised to react in lawful ways – and believe in hope. “We have faith to do things in a way that was lawful and it makes me sad what people do when they feel without hope, and there is hope,” Newsom says. “Non-violence does work - we need to include everyone to make this world a better place.” Newsom graduated from Lincoln in 1969 and describes her experience at Lincoln as, “I grew up and did more growing at Lincoln than I think I did for the rest of my life.”

A FUNÇÃO SOCIAL DA ESCOLA NO OLHAR DOS DIFERENTES SEGMENTOS DA EQUIPE ESCOLAR

O objetivo deste trabalho é levantar os diversos entendimentos que se tem sobre a função social da escola e como os diversos segmentos da equipe escolar a percebem. Procede-se a um levantamento da evolução da instituição escolar ao longo dos períodos históricos, define-se a escola como espaço socialmente destinado à transmissão do conhecimento acumulado pela sociedade humana para as novas gerações, busca-se em Libâneo e Mizukami caracterizar, para cada uma das tendências/abordagens pedagógicas, a sua função social entendendo-a nos níveis micro, meso e macro, e como uma teia de relações caleidoscópicas . Busca-se no pensamento de Gramsci os referenciais teóricos e corpo conceitual que possibilitem a leitura e compreensão dos elementos analisados e presentes na fala dos sujeitos pertencentes a segmentos da equipe escolar pesquisada. Procedeu-se a um estudo qualitativo do tipo etnográfico, através de entrevistas semi-estruturadas, observação de campo e análise documental. Verificou-se que a escola apresenta em seu Projeto Pedagógico uma forte tendência tradicional liberal, e nas falas dos sujeitos nvolvidos na pesquisa: alunos, pais/mães, colaboradores e professores uma forte crença na escola como possibilitadora de sucesso e ascensão social aos educandos, depositando nela todas as expectativas nesse sentido. O entendimento da função social da escola é percebida no nível do individual, não demonstrando preocupações com o coletivo, nem tampouco com o diploma sem saberes aprendidos , a personalidade do educando ou seu nível para o acesso ao capital cultural. Os professores partilham dessa esponsabilidade com a formação e sucesso do indivíduo educando. Buscou-se a partir dessa reflexão, um entendimento sobre a função social da escola contemporânea.

A FUNÇÃO SOCIAL DA ESCOLA NO OLHAR DOS DIFERENTES SEGMENTOS DA EQUIPE ESCOLAR

O objetivo deste trabalho é levantar os diversos entendimentos que se tem sobre a função social da escola e como os diversos segmentos da equipe escolar a percebem. Procede-se a um levantamento da evolução da instituição escolar ao longo dos períodos históricos, define-se a escola como espaço socialmente destinado à transmissão do conhecimento acumulado pela sociedade humana para as novas gerações, busca-se em Libâneo e Mizukami caracterizar, para cada uma das tendências/abordagens pedagógicas, a sua função social entendendo-a nos níveis micro, meso e macro, e como uma teia de relações caleidoscópicas . Busca-se no pensamento de Gramsci os referenciais teóricos e corpo conceitual que possibilitem a leitura e compreensão dos elementos analisados e presentes na fala dos sujeitos pertencentes a segmentos da equipe escolar pesquisada. Procedeu-se a um estudo qualitativo do tipo etnográfico, através de entrevistas semi-estruturadas, observação de campo e análise documental. Verificou-se que a escola apresenta em seu Projeto Pedagógico uma forte tendência tradicional liberal, e nas falas dos sujeitos nvolvidos na pesquisa: alunos, pais/mães, colaboradores e professores uma forte crença na escola como possibilitadora de sucesso e ascensão social aos educandos, depositando nela todas as expectativas nesse sentido. O entendimento da função social da escola é percebida no nível do individual, não demonstrando preocupações com o coletivo, nem tampouco com o diploma sem saberes aprendidos , a personalidade do educando ou seu nível para o acesso ao capital cultural. Os professores partilham dessa esponsabilidade com a formação e sucesso do indivíduo educando. Buscou-se a partir dessa reflexão, um entendimento sobre a função social da escola contemporânea.

(4-Aminomethyl)phenylguanidine derivatives as nonpeptidic highly selective inhibitors of human urokinase

Increased expression of the serine protease urokinase-type plasminogen activator (uPA) in tumor tissues is highly correlated with tumor cell migration, invasion, proliferation, progression, and metastasis. Thus inhibition of uPA activity represents a promising target for antimetastatic therapy. So far, only the x-ray crystal structure of uPA inactivated by H-Glu-Gly-Arg-chloromethylketone has been reported, thus limited data are available for a rational structure-based design of uPA inhibitors. Taking into account the trypsin-like arginine specificity of uPA, (4-aminomethyl)phenylguanidine was selected as a potential P1 residue and iterative derivatization of its amino group with various hydrophobic residues, and structure–activity relationship-based optimization of the spacer in terms of hydrogen bond acceptor/donor properties led to N-(1-adamantyl)-N′-(4-guanidinobenzyl)urea as a highly selective nonpeptidic uPA inhibitor. The x-ray crystal structure of the uPA B-chain complexed with this inhibitor revealed a surprising binding mode consisting of the expected insertion of the phenylguanidine moiety into the S1 pocket, but with the adamantyl residue protruding toward the hydrophobic S1′ enzyme subsite, thus exposing the ureido group to hydrogen-bonding interactions. Although in this enzyme-bound state the inhibitor is crossing the active site, interactions with the catalytic residues Ser-195 and His-57 are not observed, but their side chains are spatially displaced for steric reasons. Compared with other trypsin-like serine proteases, the S2 and S3/S4 pockets of uPA are reduced in size because of the 99-insertion loop. Therefore, the peculiar binding mode of the new type of uPA inhibitors offers the possibility of exploiting optimized interactions at the S1′/S2′ subsites to further enhance selectivity and potency. Because crystals of the uPA/benzamidine complex allow inhibitor exchange by soaking procedures, the structure-based design of new generations of uPA inhibitors can rely on the assistance of x-ray analysis.

Teste anti-HIV entre homens que fazem sexo com homens em São Paulo: busca espontânea rotineira e episódica

Introdução No contexto da valorização crescente do teste anti-HIV como estratégia de prevenção programática, a promoção do teste anti-HIV como estratégia de prevenção entre homens que fazem sexo com homens (HSH) é fundamental. Objetivo - Analisar os fatores associados tanto ao uso rotineiro como episódico do teste anti-HIV. Métodos - Os participantes foram 946 HSH entrevistados pelo Projeto SampaCentro em locais de sociabilidade HSH da região central de São Paulo entre novembro de 2011 e janeiro de 2012, nunca testados ou que procuraram o teste espontaneamente. A metodologia de amostragem foi a time-space-sampling e foram utilizados protocolos do Stata 12.0 para análise de amostras complexas. Os homens que se testaram por rotina ou episodicamente foram comparados aos nunca testados. As variáveis analisadas nos dois modelos de regressão de Poisson foram divididas em três níveis: características sociodemográficas (primeiro nível); socialização na comunidade gay e exposição da orientação sexual, discriminação e opiniões e atitudes em relação ao HIV/Aids e ao teste (segundo nível); percepção de risco, estratégias de prevenção e práticas e parcerias sexuais (terceiro nível). Resultados Os homens que se testaram rotineiramente eram mais velhos e moradores no Centro de SP. Além disso, tinham exposto a orientação sexual para profissional de saúde, sido discriminados em serviços de saúde mas não por amigos e/ou vizinhos (em razão da sexualidade) e não mencionaram medo do resultado do teste como motivo para HSH não se testarem. Também tinham maior probabilidade de conhecer pessoa soropositiva e de ter parcerias estáveis sem sexo anal desprotegido nas casuais (comparado a ter apenas parcerias casuais protegidas). Os homens que se testaram episodicamente eram mais velhos, residentes do Centro de SP, não moravam com parentes, expuseram sua orientação sexual para profissional de saúde, não reportaram medo do resultado do teste como barreira, conheciam pessoa soropositiva e mencionaram parceria estável sem sexo desprotegido com parceiro casual ou então sexo desprotegido em parcerias casuais (comparado a ter apenas parcerias casuais protegidas). Conclusões Os mais jovens, os que moram fora do centro de São Paulo, e os que expões menos sua orientação sexual são os segmentos que menos se testam rotineira ou episodicamente. Assim, dependem de ações para que seu direito seja protegido e assegurado. A estigmatização e a discriminação da homossexualidade deve ser combatida para que não impeça o acesso ao teste e a outros serviços de saúde. Disseminar informações e socializar os mais jovens para o diálogo sobre as estratégias de prevenção biomédicas e estratégias comunitárias de prevenção é necessário. Para ampliar o acesso e qualidade da testagem como recurso fundamental de programas de prevenção permanece o desafio de sustentar o debate sobre sexualidade e prevenção a cada geração, assim como nos programas de formação de educadores e de profissionais de saúde de todas as áreas.

Desenvolvimento de uma plataforma modelo para a busca de ligantes com potencial leishmanicida baseado na inibição seletiva da enzima diidroorotato desidrogenase

As doenças tropicais negligenciadas (DTNs) causam um imenso sofrimento para a pessoa acometida e em muitos casos podem levar o indivíduo a morte. Elas representam um obstáculo devastador para a saúde e continuam a ser um sério impedimento para a redução da pobreza e desenvolvimento socioeconômico. Das 17 doenças desse grupo, a leishmaniose, incluindo a leishmaniose cutânea, tem grande destaque devido sua alta incidência, os gastos para o tratamento e as complicações geradas em processos de coinfecção. Ainda mais agravante, os investimentos direcionados ao controle, combate e principalmente a inovação em novos produtos é ainda muito limitado. Atualmente, a academia tem um importante papel na luta contra essas doenças através da busca de novos alvos terapêuticos e também de novas moléculas com potencial terapêutico. É nesse contexto que esse projeto teve como meta a implantação de uma plataforma para a identificação de moléculas com atividade leishmanicida. Como alvo terapêutico, optamos pela utilização da enzima diidroorotato desidrogenase de Leishmania Viannia braziliensis (LbDHODH), enzima de extrema importância na síntese de novo de nucleotídeos de pirimidina, cuja principal função é converter o diidroorotato em orotato. Esta enzima foi clonada, expressa e purificada com sucesso em nosso laboratório. Os estudos permitiram que a enzima fosse caracterizada cineticamente e estruturalmente via cristalografia de raios- X. Os primeiros ensaios inibitórios foram realizados com o orotato, produto da catálise e inibidor natural da enzima. O potencial inibitório do orotato foi mensurado através da estimativa do IC50 e a interação proteína-ligante foi caracterizada através de estudos cristalográficos. Estratégias in silico e in vitro foram utilizadas na busca de ligantes, através das quais foram identificados inibidores para a enzima LbDHODH. Ensaios de validação cruzada, utilizando a enzima homóloga humana, permitiram identificar os ligantes com maior índice de seletividade que tiveram seu potencial leishmanicida avaliado in vitro contra as formas promastigota e amastigota de Leishmania braziliensis. A realização do presente projeto permitiu a identificação de uma classe de ligantes que apresentam atividade seletiva contra LbDHODH e que será utilizada no planejamento de futuras gerações de moléculas com atividade terapêutica para o tratamento da leishmaniose. Além disso, a plataforma de ensaios otimizada permitirá a avaliação de novos grupos de moléculas como uma importante estratégia na busca por novos tratamentos contra a leishmaniose

Vaccinology: The name, the concept, the adjectives

The visibility of the term vaccinology has become more pronounced in the 21st century in defining a scientific field that has absorbed aspects from different scientific domains until finally acquiring an identity of its own. As a result, vaccinology brings together a long tradition of researchers who have operated within a linear paradigm and incorporates new generations of scientists who have forged an exciting and diverse network of knowledge within this field. The term vaccinology, which initially appeared in isolation at the time of Jenner and once again with the emergence of the Pasteurian model, acquired further prominence thanks to the efforts of the vaccinologists who chronicled the production of vaccines in the last third of the 20th century. The term has since become truly consolidated, with the appearance of new adjectives during this century. This study provides a historical perspective for the frequency of use and evolution of this increasingly widespread term.

A mulher Evangélica Congregacional em Angola Análise do processo da formação pastoral da mulher no período de 1965-1975

Esta dissertação tem por objetivo analisar a mulher congregacional, sua formação pastoral no período de 1965-1975, considerado de grande desenvolvimento da Igreja. Fez-se analise dos contextos social, político, econômico e religioso para ter idéia do contexto geral do espaço em que a Igreja Evangélica Congregacional em Angola foi implantada. Falou-se da viagem dos primeiros missionários norte-americanos para o interior do país, assim como do episódio acontecido durante a implantação da Igreja no Bailundo, sede do reino na época, liderado pelo rei Ekuikui II nos primeiros anos de vida da Igreja. O desenvolvimento da igreja mereceu um olhar especial, assim como os modelos pastorais, a cooperação dos nativos na expansão do evangelho, as instituições da Igreja e sua mudança de nome. Destacaram-se as relações de gênero, a formação na cultura, o processo da formação pastoral das mulheres e os principais cursos ministrados nos centros, nas estações missionárias e no Dôndi. Os cursos eram: economia doméstica, alfabetização, educação cristã, formação feminina, formação das parteiras. A contribuição das mulheres para o desenvo lvimento da Igreja e da sociedade era diversificada, o que acontece até hoje, entretanto, destacam-se as contribuições das mulheres leigas, diaconisas, esposas dos pastores e o programa do Fogo Cristão implementado por elas. Apontei as dificuldades que as mulheres encontraram no processo da formação. Analisei a situação geral e a necessidade da formação pastoral das mulheres nos dias de hoje no Huambo e em toda Angola. O trabalho faz propostas de formação pastoral das mulheres que podem servir de ponte entre a nova e a velha geração.