981 resultados para harvest time


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Pós-graduação em Zootecnia - FMVZ

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Agronomia (Proteção de Plantas) - FCA

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Pós-graduação em Agronomia - FEIS

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Agronomia (Energia na Agricultura) - FCA

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Agronomia (Horticultura) - FCA

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Objective To assess the expression of rabies virus G-glycoprotein (RVGP) expression using Semliki Forest virus as a vector in combination with BHK-21 cells cultured in suspension. Results A multilevel factorial design was used to quantify effects of temperature (33–37 C), fresh medium addition after the viral adsorption step (100–200 % with respect to the initial cell suspension volume before infection) and harvest time (8–40 h) on RVGP production. Experimental runs were performed in 24-well cell culture plates at a multiplicity of infection (MOI) of 16. An additional experiment in spinner-flask was performed at MOI of 9, using the optimal conditions determined in cell culture plates. Values for temperature, fresh medium addition and harvest time of 33 C, 100 % and 16 h, respectively, ensured the optimal RVGP production in culture plates. The volumetric yield (239 ng ml-1 ) in these conditions was higher than that reported previously for adherent cell culture. In spinner-flasks, the volumetric yield was improved (559 ng ml-1 ). Conclusion These results establish the basis for designing bioprocess to produce RVGP.

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Japanese cornmint, also known as menthol mint (Mentiza canadensis L. syn M. arvensis L.), is an essential oil crop cultivated in several countries in Asia and South America. The plant is currently the only commercially viable source for natural menthol as a result of the high concentration of menthol in the oil compared with other crops. The hypothesis of this study was that harvesting at regular intervals within a 24-hour period would have an effect on essential oil concentration and composition of Japanese cornmint grown at high altitude in northern Wyoming. Flowering plants were harvested every 2 hours on 7 to 8 Aug. and on 14 to 15 Aug. and the essential oil was extracted by steam distillation and analyzed by gas chromatography mass spectroscopy (GC-MS). The effects of harvest date (Harvest 1 and Harvest 2) and harvest time (12 times within a 24-hour period) were significant on oil concentration and yield of menthol, but only harvest date was significant on the concentration of menthol in the oil. The interaction effect of harvest date and harvest time was significant on water content and on the concentrations of menthol and menthofuran in the oil and on the yield of limonene, menthol, and menthofuran. Overall, the oil concentration in grams per 100 g dried material for the two harvests (1.26 and 1.45, respectively), the concentration of menthol in the oil (67.2% and 72.9%, respectively), and menthol yield (1066 to 849 mg/100 g dried biomass) were higher in plants at Harvest 2 as compared with plants at Harvest 1. The oil concentration was higher in plants harvested at 1100 HR or at 1300 am and lowest in the plants harvested at 1500 BR. Menthol yield was the highest in plants harvested at 1300 HR and lowest in the plants harvested at 0700 HR, 1900 am, or at 0300 HR. This study demonstrated that harvesting time within a 24-hour period and harvest date (maturity of the crop) may affect essential oil concentration and composition of Japanese cornmint grown at high altitude in northern Wyoming.

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Pós-graduação em Zootecnia - FMVZ

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The objective of this work was to evaluate the energy and economic efficiency indexes per unit of cotton agro-ecosystem area in family production systems of Paraguay and Brazil; and, to establish a relationship between the energy and economic. Typologies presented by the Program to Support Small Cotton Holdings (Paraguay), and by the National Program for Strengthening Family Agriculture (Brazil). Family systems of the two countries were identified; these are located from Paraguay (San Juan–CA) and from Brazil (Leme–SP). To construct the energy expenditure structure of the cotton agro-ecosystem, as well as to assess the economic efficiency, the mean values obtained were considered, when they presented similarities in production systems and they were within the typology proposed in this study. From the technical itinerary observed the Paraguayan agro-ecosystem depended (fossil fuel 56.76%) and industrial source (35.99%). Thus, the energy balance of the agricultural stage was established, which attained a value of 17,740.69 MJ ha-1; an energy efficiency of 5.28, and a cultural efficiency of 3.04. The Brazilian agro-ecosystem depended on energy from industrial source (insecticides 39.82%) and from fossil fuel (33.59%); it reached an energy balance of 19,547.88 MJ ha-1; an energy efficiency of 2.12, and a cultural efficiency index of 0.71. In the economic and energy indicator ratio, with regard to the months referring to the harvest time, that is to say, March, April, and May, the maximum economic efficiency indicator of paraguay was attained in the month of May (1,00), and from Brazil in the month of May (1,71). Both production systems analyzed were presented efficient, however, dependent of external circumstances and non-renewable energy sources.