Desenvolvimento morfolôgico dos ovários em embriões e fetos bovinos da raça Nelore

The morphologic events related to the prenatal development of the ovaries in 81 Nelore breed embryos and fetuses gathered in a local slaughterhouse, with age range from 26 to 240 days following fecundation were studied. The age of fetuses was estimated from measures taken in the cranium-caudal direction. The sex was identified from macroscopic observations and using Polymerase Chain Reaction (PCR) technique. For histology the gonads were fixed in Bourn's fluid for 24 hours and 5 μm thick section's were stained with hematoxylin-eosin. Formation of gonadal ridge and presence of germinal cells were found within it at 29 and 34 days, respectively. Oogonia and primordial follicles, unlike the growing follicles, exhibited significant differences in diameter in the various periods studied. Positive correlation (P<0.05) was found between the diameter of oogonia and their nucleus as well as between primordial and growing follicles with their oocytes and respective nuclei. The gonad was fully formed at 40 days. Primordial follicles, in the growing stage, and antral follicles first appeared, approximately at 95, 140, and 180 days, respectively. Despite the onset and duration of oogenesis being similar to that of taurine breeds, folliculogenesis initiates at an early stages in the Nelore breed.

Efeitos de diferentes sistemas de cultivo in vitro sobre o crescimento de folículos pré-antrais isolados de ovários de fetos bovinos

The objective of this study is to use different in vitro culture systems of preantral follicles from Nelore breed bovine fetuses in the last gestation quarter. The evaluation of treatments considered the time of growth of isolated follicles. Preantral follicles were mechanically isolated and submitted to the individual culture, for 9 days, in media no supplemented or supplemented with fetal calf serum (FCS), bovine serum albumin (BSA) or synthetic defined supplement substitute of serum KnockoutSR (KNO). We have also evaluated the effects of collagen gel or fetal calf fibroblast monolayer as substratum for in vitro cultures. The increase on the follicular diameter was followed in the first day (0 h), at the 72 h, 144 h and 216 h. Considering cultures of isolated follicles, the results have shown that the association between media supplemented with FCS and collagen gel was significantly more efficient on the increase of the follicular diameter than other treatments. It is not still established a system of appropriate cultivation that sustains the differentiation and multiplication of the granular cells and that maintains the contact of the same ones with the oocyte to provide molecules and factors that supply the metabolic demand. We also understand that our results also represent another promising step on the search for the ultimate system of in vitro culture of preantral follicles from bovines.

Morfogênese do testículo de embriões e fetos de vacas da raça nelore (Bos taurus indicus)

The aim of this study was to accompany the process of testicular development from the non-differentiable phase to its complete formation. Embryos and fetuses of Nelore breed cows (Bos Taurus indicus) were obtained in slaughterhouses near the Uberlandia city, Minas Gerais. The gonads and the embryos were fixed in Bouin's fixative and afterwards processed for conventional optical microscopy. The gonadal was observed firstly in a 1.0 cm long embryo. In 2.5 cm long embryos the presence of the albuginea allows the sex identification. The mean thickness of the albuginea ranged from 29.08 to 558.45 mm. Gradually increase of vascularization of the albuginea and parenchyma is observed. The mediastinum is located centrally. There was a decrease in the space occupied by the testicular cords, from 63.71 to 41.99% of the total testes volume. Its diameter ranged from 31.68 to 48.80 mm. The diameter of germinal cells (and their nuclei) was from 12.27 (6.65) to 16.95 914.21) mm. The quantity of germinal cells by cross section of cord decreased from a maximum of 2.80 to 0.76. The total number of germinal cells was from 16 at the beginning of colonization of the gonad to 18.32 x 106 at the end of the study. The number of Sertoli's cells by cross section of cord ranged from 10.00 to 16.25. The results obtained show that the origin and formation of testes in embryos and fetuses from Nelore breed cows (Bos taurus indicus) does occur in a very similar way to what is described for Bos taurus taurus.

Morfologia do seio coronário em fetos de bovinos azebuados

The coronary sinusin fetuses ofzebu-crossedbovinesis studiedbyvasculardissectionsand radiographs. There aretwopredominant patterns offormation of thecoronary sinus: bydirect continuation of theleft azygos vein, from thegreat cardiac vein, and by theunion of thegreat cardiac veinwith theleft azygos vein. The first patternappeared in17animals,while thesecondpatternformation of thecoronary sinuswas found in13animals. Regarding sex there was nodifference between thepatterns shown.The main tributariesof the coronaryveinincattleare representedby the followingvessels: great cardiac vein, middle cardiac vein, veins of the faceof theleft ventricle andatrium, obliqueveinof left atrium.The great cardiac veinisseen asadirect continuation of theinterventricular veinparaconal. From these results, it appears thatthe standardvenousfetalbovineheartis predominantlyconfluentthe coronary sinus. Onlyasmall number ofvesselsare directlyconfluentto theright atrium ofbovine heart.

Expressão do fator de crescimento fibroblástico 10 (FGF-10) em folículos pré-antrais e antrais jovens durante o desenvolvimento ovariano em fetos bovinos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Anticorpos virusneutralizantes para o genótipo 1 e 2 do vírus da diarréia viral bovina em vacas gestantes abatidas em frigorífico e respectivos fetos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Gasometria da veia umbilical em fetos centralizados

Pós-graduação em Ginecologia, Obstetrícia e Mastologia - FMB

Comparação entre o volume renal em fetos de gestantes hiperglicêmicas e o volume renal em fetos de gestantes normoglicêmicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Morfogênese do testítulo de embriões e fetos de vacas da raça Nelore (Bos taurus indicus)

Pós-graduação em Zootecnia - FCAV

Desenvolvimento morfológico dos ovários em embriões e fetos bovinos da raça Nelore

Pós-graduação em Zootecnia - FCAV

Epigenética do desenvolvimento em bovinos: DNA metiltransferases e genes imprinted em embriões, fetos e placentas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Interação núcleo-citoplasmática em embriões e expressão de genes imprinted em fetos bovinos produzidos in vivo, in vitro e partenogenéticos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Composição bioquímica e hormonal do líquido ammniotico de fetos bovinos oriundos de inseminação artificial(IA), transferência de embriões(TE) e produção in vitro(PIV)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estrutura populacional de fetos arborescentes (Cyatheaceae, monilophyta) no parque estadual Carlos Botelho, SP

As samambaias arbóreas têm alta representatividade dentro do ecossistema de Mata Atlântica, mas poucas são as indicações de estudos a respeito da ecologia dessa guilda vegetal. No presente trabalho fez-se um levantamento das espécies ocorrentes na parte alta do Parque Estadual ―Carlos Botelho‖, em vista da ausência na literatura de diferenciações e quantificações de suas populações para o Parque. Definiu-se a distribuição dos indivíduos por classes de altura e a organização espacial, como elementos para a descrição das populações de samambaiaçus ocorrentes na área, destacando-se alguns parâmetros do ambiente físico e as diferenças na paisagem, que favorecem o seu desenvolvimento. Foram demarcadas 60 parcelas de 5 x 20m, em três trilhas distintas, sendo alocadas 30 parcelas no ambiente de fundo de vale e 30 na encosta. Foram identificados e medidos todos os fetos arborescentes que apresentavam troncos visíveis, sendo distribuídos em classes de altura. Para a avaliação da distribuição espacial utilizou-se a Relação Variância/Média, o Índice de Morisita e o Índice de Morisita Padronizado. Os parâmetros de habitat medidos foram a cobertura de dossel e a umidade do solo e da serrapilheira. Através do teste T-student comparou-se as densidades das populações entre o fundo de vale e a encosta, assim como as diferenças entre os parâmetros de habitat medidos em cada ambiente e trilha. Foram amostrados 247 indivíduos, sendo 83 de Alsophila setosa, 63 de Cyathea phalerata, 32 de C. delgadii, 11 de C. dichromatolepis, 10 de A. sternbergii e 4 de C. corcovadensis. A maioria das espécies apresentou um maior número de indivíduos nas classes de menor tamanho, indicando que as populações possuem grande capacidade de regeneração natural na área. Todas as espécies apresentaram padrão de ...(Resumo completo, clicar acesso eletrônico abaixo)

Freqüência do parvovírus suíno em fetos mumificados, abortados e natimortos detectado através da reação em cadeia pela polimerase (PCR)

Porcine parvovirus (PPV) is associated with reproductive failure and it has been found worldwide, including Brazil. Many diagnostic procedures are used for its detection, for example, immunofluorescence, HA, HI and PCR and this is an important technique because it is very specific and sensitive. In this work, the presence of PPV in fetuses from swine farms with reproductive problems was detected by PCR. All of 170 samples from aborted fetuses, mummies or stillborns were sampled by PCR with primers designed to VP2 region of PPV and c-myc (endogenous control). Only 142 samples (83,53%) were positive for c-myc and among them six samples (4,22%) were positive for PPV which were tested in HA. In this test, erythrocytes suspension 1% was used and three samples (50%) agglutinated but they presented low titer (4). For this work, porcine parvovirus was detected in the samples analyzed by PCR and HA. It is important to emphasize the use of endogenous control when material with elevated degree of autolysis is examined