Atividade de fosfomonohidrolases presentes em fígado de girino de rã-touro (Lithobates catesbeianus) durante a metamorfose

Pós-graduação em Biotecnologia - IQ

Multistep taphonomic alterations in fluvial mollusk shells: a case study in the touro passo formation (pleistocene–holocene), Southern Brazil

Accurate paleoenvironmental reconstruction relies on the correct interpretation of the postmortem history of skeletal remains in shelly assemblages. In contrast to marine settings, actualistic taphonomic studies are lacking for shell-rich concentrations in freshwater riverine systems. In particular, the taphonomic pathways and the origins of taphonomic signatures that are recorded in bioclasts from fluvial settings are poorly known. In this study, we addressed this issue by comparing the taphonomic signatures and shell-damage profiles among shells of freshwater mollusks recorded both in death and in fossil assemblages from the same fluvial environment. Our data indicated that dissolution was the most pervasive taphonomic process leading to the destruction of the shells. The loss of taphonomic information extended beyond shell dissolution in the riverbed, or the early diagenesis in the sedimentary record. The loss of biological information from the living community through the death assemblage, until the incorporation of shells as fossils, mainly occurred during the time the shells were in the sediment-water interface. Though this destruction affected primarily dead shells, reworked fossils also became vulnerable because they were carried out into the river load again by channel avulsion. A model that included the main taphonomic pathways followed by the molluscan shells in the fluvial Touro Passo Formation (Pleistocene-Holocene) is discussed. In this model, two main destructive domains were recognized, which were the biological, physical, and chemical processes operating at the taphonomically active zone (= TAZ domain) and the pedogenetic domain.

Tiouréia no desenvolvimento de girinos de rã-touro e no desempenho de animais pós-metamórficos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Digestibilidade e exigência de aminoácidos para rã-touro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Recria de Rã touro (Rana catesbeiana) em tanques-rede alojados em viveiros de tilápia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Retorno à atividade reprodutiva de touro angus após fimose traumática com obliteração parcial da luz prepucial: relato de caso

The main disturbs affecting the genital segment of bulls are balanitis, phimosis and preputial injuries, abscesses or prolapses, resulting in decreased libido or mating ability. One case of traumatic phimosis with partial obliteration of preputial lumen is reported in Aberdeen Angus bull. During anamnesis, it was reported that the animal was unable to expose the penis and presented increased penile and preputial volume of unknown etiology after being submitted to a libido test. During clinical examination, a firm preputial mass of 15 cm in diameter was observed in the middle third of the prepuce and stenosis of preputial lumen was detected approximately 17 cm from the preputial orifice. Reconstructive surgery was the chosen therapy, with amputation of the affected foreskin portion. A skin segment of about 12 cm and affected mucosal portion were removed. Postoperative therapy consisted of dressing with chlorhexidine digluconate based ointment, povidone iodine and topical insect repellent (cypermethrin and carbamyl), systemic antibiotic therapy with benzathine penicillin (30,000 IU / kg, repeated after 48 hours) and anti-inflammatory therapy with flunixin meglumine (2 , 0 mg / kg SID) for three days. After twenty days, semen was collected by massage of the accessory glands. The ejaculate volume was 3 mL, with 200x106 spermatozoa / mL, white color, "suis generis" odor, 88% of motile sperm, 64% of progressively motile sperm and 82% of rapid sperm. Thus, the chosen surgical treatment was effective to correct the pathology and reintroduce the animal into reproductive activity

Confirmation Service : as sung at Touro Synagogue, consisting of six hymns, responses, etc., for solo, trio and chorus

publ. by Fred. E. Kitziger

Desenvolvimento biotecnológico de uma emulsão de uso tópico a base de óleo de rã-touro Rana catesbeiana Shaw

The skin is one of the largest organs of the human body and accounts for about 16% of body weight. The body protection against the external environment microorganisms is one of its most important functions, however is necessary that the skin remain intact for this function be exercised, so that when there is an injury on the skin, the process of restructuring needs to be starts, however this restructuration may also be compromised due to some diseases, justifying even more the need for the development of topical products that promote or accelerate the skin healing. Thus the aim of this study was to extract bullfrog oil and to develop a suitable topical emulsion. Two different oil samples were extracted by hot or organic solvent process. Titration techniques and gas chromatography- mass spectrometry were used to characterize the bullfrog oil. The required hydrophile-lipophile balance (HLBr) of bullfrog oil was determined and a pseudo-ternary phase diagram was constructed. The stability of the topical emulsion was evaluated. Then, cellular viability was determined by MTT assay using normal fibroblasts (3T3) and melanoma (B16F10) cells lines. The hot extraction yield was 60.6%. The major polyunsaturated compounds found were Eicosapentaenoic acid (17.6%) and Arachidonic acid (8.4%). HLBr study demonstrated the presence of stable systems with HLB ranging from 12.1 to 13.5 and the pseudo-ternary phase diagram showed mainly emulsion systems (62%). Topical emulsion showed 390 nm, polydispersity 0.05, zeta potential -25 mV and remained stable for ninety days. The bullfrog oil and topical emulsion did not showed citotoxicity in normal fibroblasts cells. However, these systems showed significantly inhibition of melanoma cells growth. In conclusion, the bullfrog oil presented desirable chemical characteristics required to be used for the development of a pharmaceutical and cosmetic products.

Adição de Ácido Linoleico conjugado (CLA) no diluidor de congelação de sémen de touro, recolhido Post-Mortem, das raças Holstein Frísia, Aberdeen Angus, Charolês e Limousine

Dissertação de Mestrado, Engenharia Zootécnica, 7 de Abril de 2016, Universidade dos Açores.

O touro na mitologia egípcia : Ápis, cerimónias, insígnias e epítetos reais

Os antigos Egípcios acreditavam que o touro poderoso representava a personalidade do próprio faraó. O touro estava, de facto, intimamente associado ao Estado faraónico, estando presente ao nível dos regalia (cauda taurina) e significativamente nos próprios epítetos reais (ka nakht). Na mitologia egípcia, de todos os touros sagrados o que maior projecção alcançou, como deus agrário da fecundidade, da vegetação renascida e da ressurreição, foi, seguramente, o touro Ápis, associado em Mênfis aos deuses Ptah e Osíris. Na sua condição de touro ágil, vigoroso e viril, Ápis era um intermediário consistente entre o mundo dos vivos e o dos mortos, além de ser um propiciador de fertilidade e renascimento quando associado ao deus-Sol. A sua participação, literalmente ao lado do faraó, na «corrida ritual», importante cerimónia no âmbito da concepção ideológica do poder real, reforçou ainda mais a sua importância no seio do panteão egípcio.

Inclusão de farinha de resíduos da indústria de filetagem de tilápias na alimentação de girinos de rã-touro (Rana Catesbeiana Shaw,1802)

The objective of this experiment was to evaluate the inclusion of meal of tilapia industrial waster in bullfrog tadpoles' diets (Rana Catesbeina Shaw, 1802). Two hundred fifty tadpoles with initial weight and length of 0,18 +/- 0,05g and 2,50 +/- 0,18 centimeters were used, respectively, they were used and distributed in 25 aquariums of 30 liter each in a complete randomized design with five treatments and five repetitions. The tadpoles were fed four times a day with diets containing 0, 5, 10 and 15% of inclusion of tilapia filleting and another diet with 0% of supplemented tilapia filleting with synthetic methionine, being supplied four times a day at ease. The experiment period was 30 days. The diets with tilapia filleting inclusion and methionine supplementation did not have influence in the final weight and length averages, feed survival and conversion of bullfrog tadpoles. It cam be used until 15% of tilapia filleting inclusion in the bullfrog tadpoles food. The synthetic methionine supplementation in diets for bullfrog tadpoles is not necessary in diets with levels over 0,471/4 of the total methionine.

Isolamento e resistência a antimicrobianos de cepas de Streptococcus spp. provenientes de rãs-touro (Lithobates catesbeianus)

Twelve bullfrogs were selected from two commercial frog farms and clinically diagnosed as attacked by Streptococcus disease. Sixty samples were collected, and Streptococcus spp. was isolated from all bullfrog, being 12 (100%) from the encephalus, seven from the kidneys (58.3%), three from the liver (25%), two from the spleen (16.6%), and one from the ascitic liquid (8.3%). Streptococcus -hemolytic were isolated from all the 60 samples, which were sensible to chloramphenicol (100%), gentamycin (100%), vancomycin (96%), cefotaxime (96%), and cefoxitine (92%).