Binding of N-dansylgalactosamine to winged-bean tuber lectin: studies by fluorescence quenching titrations

The winged-bean tuber lectin binds to N-dansyl(5-dimethylaminonaphthalene-1-sulphonic acid)galactosamine, leading to a 12.5-fold increase in dansyl fluorescence with a concomitant 25 nm blue-shift in the emission maximum. The enhancement of fluorescence intensity was completely reversed by the addition of methyl α-galactopyranoside. The lectin has two binding sites per molecule for this fluorescent sugar and an association constant of 2.59 · 105 M−1 at 25° C. The binding of N-dansylgalactosamine to the lectin shows that it can accommodate a large hydrophobic substituent on the C-2 carbon of d-galactose. Studies with other sugars indicate that a hydrophobic substituent with α-conformation at the anomeric position increases the affinity of binding. The C-4 and C-6 hydroxyl groups are also critical for sugar binding to this lectin.

Portrait of the family of David Stern and Bernice Stern nee Propper.

Seated: David Stern, Bernice Stern, and Jessica Agosti; left to right: Doreen Stern, her husband Michael Stern, Carol Richardson, her husband Blake Richardson, John Agosti. The baby is Katherine Stern

Ruth Weiss nee Stern with Annette, Meir, and David Weiss at Mortimer Hill Farm; Mortimer, England.

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Eva Blumenau nee Stern (far left) during a gathering of Holocaust survivors; Moenchengladbach (Rheydt), Germany.

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Alice Burlin nee Molling, Therese Gottschalk nee Molling, and Line Stern nee Wallerstein at Alice Burlins's home; Linton near Cambridge, England.

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Studio portrait of Liselotte "Lotte" Stern nee Wallerstein.

Photograph probably taken shortly before her marriage to Alfred Stern in 1928.

Lotte Stern and Dr. Alfred Stern with Otto Wallerstein, a relative of Lotte's father; Berkeley, California.

From left to right: Lotte Stern, Otto Wallerstein, Alfred Stern; Photograph taken shortly after arrival in Berkeley, California

Ruth Weiss nee Stern with Annette, Meir, and David Weiss at Mortimer Hill Farm; Mortimer, England..

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Studio portrait of Nanny Stern (Oberglen) at eighty-four years old; Frankfurt.

Grandmother of Fred Herz; Born 27 September 1845, died 9 May 1929

Portrait of Kathinka Herz nee Stern outdoors.

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Paula Stern nee Rosenbaum and Maier Stern standing outdoors in a field Portraits Couples

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Eva Herzberg later Stern (left) with two unidentified young women Portraits Groups Women

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Signed portrait of Eva Herzberg later Stern Portraits Groups Women

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Inhibition of self-quenching in thioketones by micellar compartmentalization

The technique of micellar compartmentalization has been used to inhibit the diffusion-controlled self-quenching process in thioketones. By adjusting the ratio of the bulk concentration of the thioketone solute to the bulk concentration of micelles multiple occupancy of the micelles was avoided. Under these conditions enhanced phosphorescence intensity was observed in nitrogen-purged micellar solutions compared with that in acetonitrile solutions, indicating that the thioketone triple was indeed protected from deactivation by a ground statet

Edna and Moses Stern with daughters Erna and Annie Portraits Family

Grandparents of Margot Gerson