Sobre el uso terapéutico de células estaminales de origen adulto

Resumen: Muy pocas ciencias avanzaron con la celeridad que muestran las ciencias biológicas. Hoy se abren líneas de investigación que, en poco tiempo, se aplican a los pacientes, mejorando notablemente -en la mayoría de los casos- la calidad de vida. Las terapias con células estaminales son un claro ejemplo de ello. Hoy el tema se ha instalado en la sociedad como parte de la agenda pública. Esta exposición ha llevado a la sociedad a una recepción con un lamentable doble riesgo: perder rigor científico y generar falsas expectativas en muchos pacientes. Dicha polémica también afecta las relaciones entre diversos grupos de investigadores y genera desconfianza en la comunidad científica. Desde la última mitad del siglo XX se ha instalado en las ciencias la necesidad de no escindir el campo de la investigación de las consecuencias éticas que siguen de esa praxis. La causa es evidente: la celeridad de los descubrimientos y su rápida aplicación hacen que, así como los efectos positivos alcanzan a una gran cantidad de personas, en caso de surgir efectos negativos imprevistos, el daño pueda ser igualmente multiplicado. La bioética surge así como un reclamo de los mismos investigadores que decidieron dar un cauce ético a su acción.

La demanda de telefonía fija y móvil: Una aplicación de redes neuronales artificiales

[ES] El catalogar a un determinado bien o servicio como una de las «extensiones de los sentidos y las funciones humanas de la vista, el oído y el tacto», muestra la importancia del papel que desempeñan en nuestras vidas, muestra el desarrollo que alcanzaron impulsadas por las mismas necesidades de los seres humanos, muestra un mercado dinámico e importante. El servicio de telefonía móvil o celular es el desencadenador de estas expresiones y además tema de comentario, investigación y preocupación de comunidades científicas y organismos internacionales como el World Economic Forum de Davos.

Communities in complex networks

The study of complex networks has attracted the attention of the scientific community for many obvious reasons. A vast number of systems, from the brain to ecosystems, power grid, and the Internet, can be represented as large complex networks, i.e, assemblies of many interacting components with nontrivial topological properties. The link between these components can describe a global behaviour such as the Internet traffic, electricity supply service, market trend, etc. One of the most relevant topological feature of graphs representing these complex systems is community structure which aims to identify the modules and, possibly, their hierarchical organization, by only using the information encoded in the graph topology. Deciphering network community structure is not only important in order to characterize the graph topologically, but gives some information both on the formation of the network and on its functionality.

Mass Energy and Flow in closed ecosystems

The general equations of biomass and energy transfer for an n-species, closed ecosystem are written. It is demonstrated how in "ecological time" the parameters describing the dynamics of biomass transfer are related to the parameters of energy transfer, such as respiration, fixation, and energy content. This relationship is determinate for the straight-chain ecosystem, and a simple example is worked out. The results show how the density dependent terms in population dynamics arise naturally, and how the stable system exhibits a hierarchy in energy per unit biomass. A procedure is proposed for extending the theory to include webbed systems, and the particular difficulties involved in the extension are brought before the scientific community for discussion.

Report on the pinniped and sea otter tagging workshop 18-19 January 1979 Seattle, Washington

The Marine Mammal Tagging Office has been created by consensus of the agencies responsible for marine mammal management and the scientific community dealing with marine mammal tagging and marking. The purpose of ths office is to facilitate the dissemination of information with regard to tagging, marking, tags, and marks; to determine the need for new and better materials for tags; and to stimulate research, development, and testing programs. The American Institute of Biological Sciences was requested to coordinate a workshop to determine the status of pinniped tagging both nationally and internationally. Approximately 30 scientists were invited to participate in the workshop which was held on 18-19 January 1979 at the Sand Point Laboratory of the National Marine Fisheries Service in Seattle, Washington. Topics included ranged from specific tagging programs to general considerations and similar problems encountered by researchers. Participants also participated in one of three working groups -- Sea Otters, Phocids, and Otariids --to address pertinent issues. These break-out sessions resulted in the general recommendations and specific considerations sections of this report. Abstract authors include: Alton Y. Roppel; Ken Pitcher; Burney J. Le Boeuf; Wybrand Hoek; Robert M. Warneke; Don B. Siniff; Doug P. DeMaster; Daniel J. Miller; Ian Stirling; Roger L. Gentry; Lanny H. Cornell; James E. Antrim; Edward D. Asper; Mark Keyes; R. Keith Farrell; Donald G. Calkins; Bob DeLong; T. A. Gornall; Tom Otten; and, Ancel M. Johnson (PDF contains 54 pages)

A Surfboard for Riding the Wave

The report, A Surfboard for Riding the Wave, by the high level Knowledge Exchange expert group on research data called for a collaborative data infrastructure that will enable researchers and other stakeholders from education, society and business to use, re-use and exploit research data to the maximum benefit of science and society. The Knowledge Exchange partners have embraced this vision and commissioned a report that translates Riding the Wave into actions for the four partner countries and beyond. This paper builds on this report and presents an overview of the present situation with regard to research data in Denmark, Germany, the Netherlands and the United Kingdom and offers broad outlines for a possible action programme for the four countries in realising the envisaged collaborative data infrastructure. An action programme at the level of four countries will require the involvement of all stakeholders from the scientific community

Widow rockfish: Proceedings of a workshop, Tiburon, California, December 11-12, 1980

This workshop was organized because of the increase between 1978 and 1980 in coastwide landings of widow rockfish, from less than 1,000 mt to more than 20,000 mt, and because of scientists' concern with the lack of knowledge both of the fishery and biology of the species. Most scientists active in research on Pacific groundfish, as well as some members of the fishing industry and fishery managers, attended the workshop. These proceedings contain the report of the workshop discussion panel, status reports on California, Oregon, and Washington fisheries through 1980, and a collection of seven papers presented at the workshop. The status reports provide an historical perspective of the development of an important fishery. The papers present a fairly complete survey of biological knowledge of widow rockfish, economic status of the fishery, and fishery-independent methods for estimation of abundance. The papers also contain some information developed after the workshop. Since the workshop, the fishery has matured. Largest landings were made in 1981, when more than 28,000 mt were landed. Maximum sustainable yield (MSY) is estimated to be slightly less than 10,000 mt, and the stock appeared to be at about the MSY level in 1985. The Pacific Fishery Management Council and National Marine Fisheries Service have implemented regulations that have maintained landings since 1983 at approximately the maximum sustainable yield level. Fishery-dependent stock assessments are being made on an annual basis for the Pacific Fishery Management Council. While these assessments are considered to be the best possible with available data, scientists responsible for the assessment have chosen to delay their publication in the formal scientific literature until more data are obtained. However, the stock assessment reports are available from the Pacific Fishery Management Council. In addition to the papers in this collection, three papers have been published on widow rockfish since 1980. BoehIert, Barss, and Lamberson (1982) estimate fecundity of the species off Oregon; Gunderson (1984) describes the fishery and management actions; and Laroche and Richardson (1981) describe the morphology and distribution of juvenile widow rockfish off Oregon. During the past decade, the fishery for widow rockfish has developed from a minor fishery to one of the more important on the Pacific Coast. Our knowledge of the biology and dynamics of the species has progressed from minimal to relatively extensive for a groundfish species. It is our intention in preparing this collection of papers to make this knowledge readily available to the scientific community. (PDF file contains 63 pages.)

Underwater Passive Acoustic Monitoring for Remote Regions, Hawaii Institute of Marine Biology, Coconut Island, Hawaii, February 7-9, 2007: workshop proceedings

The Alliance for Coastal Technologies (ACT) convened a workshop, sponsored by the Hawaii-Pacific and Alaska Regional Partners, entitled Underwater Passive Acoustic Monitoring for Remote Regions at the Hawaii Institute of Marine Biology from February 7-9, 2007. The workshop was designed to summarize existing passive acoustic technologies and their uses, as well as to make strategic recommendations for future development and collaborative programs that use passive acoustic tools for scientific investigation and resource management. The workshop was attended by 29 people representing three sectors: research scientists, resource managers, and technology developers. The majority of passive acoustic tools are being developed by individual scientists for specific applications and few tools are available commercially. Most scientists are developing hydrophone-based systems to listen for species-specific information on fish or cetaceans; a few scientists are listening for biological indicators of ecosystem health. Resource managers are interested in passive acoustics primarily for vessel detection in remote protected areas and secondarily to obtain biological and ecological information. The military has been monitoring with hydrophones for decades;however, data and signal processing software has not been readily available to the scientific community, and future collaboration is greatly needed. The challenges that impede future development of passive acoustics are surmountable with greater collaboration. Hardware exists and is accessible; the limits are in the software and in the interpretation of sounds and their correlation with ecological events. Collaboration with the military and the private companies it contracts will assist scientists and managers with obtaining and developing software and data analysis tools. Collaborative proposals among scientists to receive larger pools of money for exploratory acoustic science will further develop the ability to correlate noise with ecological activities. The existing technologies and data analysis are adequate to meet resource managers' needs for vessel detection. However, collaboration is needed among resource managers to prepare large-scale programs that include centralized processing in an effort to address the lack of local capacity within management agencies to analyze and interpret the data. Workshop participants suggested that ACT might facilitate such collaborations through its website and by providing recommendations to key agencies and programs, such as DOD, NOAA, and I00s. There is a need to standardize data formats and archive acoustic environmental data at the national and international levels. Specifically, there is a need for local training and primers for public education, as well as by pilot demonstration projects, perhaps in conjunction with National Marine Sanctuaries. Passive acoustic technologies should be implemented immediately to address vessel monitoring needs. Ecological and health monitoring applications should be developed as vessel monitoring programs provide additional data and opportunities for more exploratory research. Passive acoustic monitoring should also be correlated with water quality monitoring to ease integration into long-term monitoring programs, such as the ocean observing systems. [PDF contains 52 pages]

Aspects of quality control of wind profiler measurements in complex topography

It is well known in the scientific community that some remote sensing instruments assume that sample volumes present homogeneous conditions within a defined meteorological profile. At complex topographic sites and under extreme meteorological conditions, this assumption may be fallible depending on the site, and it is more likely to fail in the lower layers of the atmosphere. This piece of work tests the homogeneity of the wind field over a boundary layer wind profiler radar located in complex terrain on the coast under different meteorological conditions. The results reveal the qualitative importance of being aware of deviations in this homogeneity assumption and evaluate its effect on the final product. Patterns of behavior in data have been identified in order to simplify the analysis of the complex signal registered. The quality information obtained from the homogeneity study under different meteorological conditions provides useful indicators for the best alternatives the system can offer to build wind profiles. Finally, the results are also to be considered in order to integrate them in a quality algorithm implemented at the product level.

Mechanistic studies of reactions at the single-molecule level using microfluidics with applications in molecular diagnostics

Motivated by needs in molecular diagnostics and advances in microfabrication, researchers started to seek help from microfluidic technology, as it provides approaches to achieve high throughput, high sensitivity, and high resolution. One strategy applied in microfluidics to fulfill such requirements is to convert continuous analog signal into digitalized signal. One most commonly used example for this conversion is digital PCR, where by counting the number of reacted compartments (triggered by the presence of the target entity) out of the total number of compartments, one could use Poisson statistics to calculate the amount of input target.

However, there are still problems to be solved and assumptions to be validated before the technology is widely employed. In this dissertation, the digital quantification strategy has been examined from two angles: efficiency and robustness. The former is a critical factor for ensuring the accuracy of absolute quantification methods, and the latter is the premise for such technology to be practically implemented in diagnosis beyond the laboratory. The two angles are further framed into a “fate” and “rate” determination scheme, where the influence of different parameters is attributed to fate determination step or rate determination step. In this discussion, microfluidic platforms have been used to understand reaction mechanism at single molecule level. Although the discussion raises more challenges for digital assay development, it brings the problem to the attention of the scientific community for the first time.

This dissertation also contributes towards developing POC test in limited resource settings. On one hand, it adds ease of access to the tests by incorporating massively producible, low cost plastic material and by integrating new features that allow instant result acquisition and result feedback. On the other hand, it explores new isothermal chemistry and new strategies to address important global health concerns such as cyctatin C quantification, HIV/HCV detection and treatment monitoring as well as HCV genotyping.

Self-assembly of brush polymers

The unique structure and properties of brush polymers have led to increased interest in them within the scientific community. This thesis describes studies on the self-assembly of these brush polymers.

Chapter 2 describes a study on the rapid self-assembly of brush block copolymers into nanostructures with photonic bandgaps spanning the entire visible spectrum, from ultraviolet to near infrared. Linear relationships are observed between the peak wavelengths of reflection and polymer molecular weights. This work enables "bottom-up" fabrication of photonic crystals with application-tailored bandgaps, through synthetic control of the polymer molecular weight and the method of self-assembly.

Chapter 3 details the analysis of the self-assembly of symmetrical brush block copolymers in bulk and thin films. Highly ordered lamellae with domain spacing ranging from 20 to 240 nm are obtained by varying molecular weight of the backbone. The relationship between degree of polymerization and the domain spacing is reported, and evidence is provided for how rapidly the brush block copolymers self-assemble and achieve thermodynamic equilibrium.

Chapter 4 describes investigations into where morphology transitions take place as the volume fraction of each block is varied in asymmetrical brush block copolymers. Imaging techniques are used to observe a transition from lamellar to a cylindrical morphology as the volume fraction of one of the blocks exceeds 70%. It is also shown that the asymmetric brush block copolymers can be kinetically trapped into undulating lamellar structures by drop casting the samples.

Chapter 5 explores the capability of macromolecules to interdigitate into densely grafted molecular brush copolymers using stereocomplex formation as a driving force. The stereocomplex formation between complementary linear polymers and brush copolymers is demonstrated, while the stereocomplex formation between complementary brush copolymers is shown to be restricted.

ICT Applications in the Research for Environmental Sustainability

26 p.

Analysis and Use of Information and Communication Tools in Economics of Climate Change

16 p.

Avaliação das propriedades de barreira a gases de membranas obtidas a partir de dispersões aquosas à base de poliuretanos e argila

Materiais nanoestruturados têm recebido destaque na comunidade científica, destacando-se, dentre eles, os nanocompósitos à base de polímeros e argila. Quando esses materiais são obtidos no estado líquido, ressalta-se também o uso de água em substituição a solventes orgânicos, devido a questões ambientais. Neste trabalho foram sintetizadas dispersões aquosas à base de poliuretanos (WPUs) e argila hidrofílica do tipo montimorilonita (MMT) de natureza sódica, com o objetivo de avaliar as propriedades de barreira a gases conferidas pela presença de argila e pela variação nas proporções entre os segmentos flexíveis poli(glicol propilênico) (PPG) e o copolímero em bloco à base de poli(glicol etilênico) e poli(glicol propilênico) (EG-b-PG). Os monômeros empregados na síntese foram: poli(glicol propilênico) (PPG); copolímero em bloco à base de poli(glicol etilênico) e poli(glicol propilênico) (EG-b-PG), com teor de 7% de EG; ácido dimetilolpropiônico (DMPA), diisocianato de isoforona (IPDI) e etilenodiamina (EDA), como extensor de cadeia. Foram sintetizadas dispersões aquosas com e sem a presença de argila, fixando-se a razão entre o número de equivalentes-grama de grupos diisocianato e hidroxila (razão NCO/OH) em 1,5. Nas formulações foi variado também o teor de argila em relação à massa de prepolímero em 0,5% e 1%. Foi adicionada uma etapa de agitação adicional com dispersor Turrax em algumas formulações. A argila foi previamente deslaminada em água deionizada e incorporada à formulação na etapa da dispersão do prepolímero. As dispersões foram avaliadas, quanto ao teor de sólidos totais, tamanho médio de partícula e viscosidade aparente. Os filmes vazados a partir das dispersões foram caracterizados por espectrometria na região do infravermelho (FTIR) e permeabilidade ao CO2. A resistência térmica dos filmes foi determinada por termogravimetria (TG). Foram observadas modificações nas propriedades dos filmes obtidos com a inserção da argila e com a variação no teor de segmentos à base de poli(glicol etilênico). A inserção da argila promoveu uma melhoria na resistência térmica das membranas bem como uma redução na permeabilidade das mesmas. Foi observado um aumento na permeabilidade das membranas obtidas a partir das formulações com maior percentual de copolímero (EG-b-PG), com e sem argila.

Tratamento de efluentes líquidos na Estação Antártica Comandante Ferraz (EACF): avaliação da aplicabilidade do processo eletrolítico.

Esta pesquisa enfoca o uso do processo eletrolítico, como alternativa para tratamento de efluentes líquidos na Estação Antártica Comandante Ferraz (EACF), considerando as limitações ambientais locais e aspectos de consumo de energia. Este processo, classificado como não convencional, vem sendo estudado pela comunidade científica nacional e internacional para tratamento de diversos efluentes, inclusive esgotos domésticos, apresentando várias vantagens que estimularam a verificação de sua aplicabilidade para as condições peculiares da EACF. Foram realizados ensaios, em escala de laboratório, com esgotos domésticos coletados em um condomínio no Rio de Janeiro, usando reatores eletrolíticos com capacidade de 4 L, com eletrodos de desgaste de alumínio (Al) e de ferro (Fe), distância entre as placas de 0,9 cm e 1,8 cm, temperaturas na faixa de 7C a 22C, e ensaios para verificação da sua eficiência, por meio de parâmetros como DQO, DBO5, SST, turbidez e volume de lodo gerado. Sob temperatura de 15C e condições de condutividade da ordem de 900 S/cm, estimada para os esgotos da EACF, aplicando densidade de corrente de 22,9 A/m2, 4,5 V, tempo de retenção de 25 min, os resultados apresentaram valores de DQO no efluente tratado de 65 mg/L(redução de 89%), DBO de 56 mg/L (redução de 64 %), SST de 8 mg/L, com turbidez de 11,3 uT e, após filtração, turbidez de 3,2 uT, consumo de energia de 0,8 Wh/L. O aspecto é límpido e a qualidade final obtida é compatível para ser submetida a tratamento de desinfecção. A partir dos dados obtidos, foram avaliadas por meio de pré-projeto: a viabilidade de sua implantação em container, a estimativa de consumo de energia e de lodo gerado, requisitos de manutenção, operação, além da sugestão de monitoramentos e de medidas de mitigação de impactos ambientais associados à respectiva instalação.