Systematic Reverse Genetics of Transfer-DNA-Tagged Lines of Arabidopsis1 : Isolation of Mutations in the Cytochrome P450 Gene Superfamily

We have developed an efficient reverse-genetics protocol that uses expedient pooling and hybridization strategies to identify individual transfer-DNA insertion lines from a collection of 6000 independently transformed lines in as few as 36 polymerase chain reactions. We have used this protocol to systematically isolate Arabidopsis lines containing insertional mutations in individual cytochrome P450 genes. In higher plants P450 genes encode enzymes that perform an exceptionally wide range of functions, including the biosynthesis of primary metabolites necessary for normal growth and development, the biosynthesis of secondary products, and the catabolism of xenobiotics. Despite their importance, progress in assigning enzymatic function to individual P450 gene products has been slow. Here we report the isolation of the first 12 such lines, including one (CYP83B1-1) that displays a runt phenotype (small plants with hooked leaves), and three insertions in abundantly expressed genes. The DNAs used in this study are publicly available and can be used to systematically isolate mutants in Arabidopsis.

Reverse Offshoring of Services: The New Wave of Emerging Offshorers. ACES Working Papers, August 2010

Offshore outsourcing of services accelerated at the end of 1990s in developed countries. In recent years, developing countries have also offshored services, not only to developed countries but also to other developing countries. Yet, to date little attention has been paid to the emergence of this reverse offshoring. The focus of this research is on the determinants of reverse offshoring of services; as such we will investigate what drives these firms from developing countries to offshore services.

The illustrated dictionary of gardening : a practical and scientific encyclopaedia of horticulture for gardeners and botanists /

"Reference to illustrations of plants other than those figured in this work" precedes text in each volume.

The Modern world dictionary of the English language ... to which is added a compendium of historical, biographical, geographical, scientific, religious, and sociological names, and a complete summary of human achievement in the nineteenth & twentieth centuries ...

Editors-in-chief: Robert Hunter and Charles Morris.

A dictionary of chemical equations.

Mode of access: Internet.

The gentleman and lady's key to polite literature, or, A compendious dictionary of fabulous history : containing the characters, and principal actions, ascribed to the heathen gods, goddesses, heroes, &c. and the manner in which the ancients represented the deities & heroes ... : intended for the assistance of those who would understand mythology, poetry, painting, statuary and theatrical entertainments, and particularly adapted to the use of Latin and French schools.

Frontispiece signed "Le Pautre." Engraved title-page.

Biographical and bibliographical dictionary of the Italian humanists and of the world of classical scholarship in Italy, 1300-1800.

Vol. 6: Supplement. A-Z has half title: Dictionary of the Italian humanists.

Interlingua-English; a dictionary of the international language,

Mode of access: Internet.

Dictionary of Jewish public affairs and related matters /

Mode of access: Internet.

First lessons in botany and vegetable physiology : illustrated by over 360 wood engravings from original drawings by Isaac Sprague : to which is added a copious glossary, or dictionary of botanical terms /

Mode of access: Internet.

An etymological dictionary of the English language /

Spine title: Oswald's etymological dictionary.

Dictionary of sociology,.

Mode of access: Internet.

Philosophic words, a study of style and meaning in the Rambler and Dictionary of Samuel Johnson.

Appendices (p. [123] - 160: A. Index of philosophic words in the Rambler.--B. Some philosophic sources of Johnson's Dictionary.

Dictionary of United States Army terms.

Mode of access: Internet.

A dictionary of Sanscrit and English, designed for the use of private students and of Indian colleges and schools.

Mode of access: Internet.