TAK, an HIV Tat-associated kinase, is a member of the cyclin-dependent family of protein kinases and is induced by activation of peripheral blood lymphocytes and differentiation of promonocytic cell lines

We have previously identified a cellular protein kinase activity termed TAK that specifically associates with the HIV types 1 and 2 Tat proteins. TAK hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II in vitro in a manner believed to activate transcription [Herrmann, C. H. & Rice, A. P. (1995) J. Virol. 69, 1612–1620]. We show here that the catalytic subunit of TAK is a known human kinase previously named PITALRE, which is a member of the cyclin-dependent family of proteins. We also show that TAK activity is elevated upon activation of peripheral blood mononuclear cells and peripheral blood lymphocytes and upon differentiation of U1 and U937 promonocytic cell lines to macrophages. Therefore, in HIV-infected individuals TAK may be induced in T cells following activation and in macrophages following differentiation, thus contributing to high levels of viral transcription and the escape from latency of transcriptionally silent proviruses.

The dimer-dimer interaction surface of the replication terminator protein of Bacillus subtilis and termination of DNA replication.

The replication terminator protein (RTP) of Bacillus subtilis causes polar fork arrest at replication termini by sequence-specific interaction of two dimeric proteins with the terminus sequence. The crystal structure of the RTP protein has been solved, and the structure has already provide valuable clues regarding the structural basis of its function. However, it provides little information as to the surface of the protein involved in dimer-dimer interaction. Using site-directed mutagenesis, we have identified three sites on the protein that appear to mediate the dimer-dimer interaction. Crystallographic analysis of one of the mutant proteins (Y88F) showed that its structure is unaltered when compared to the wild-type protein. The locations of the three sites suggested a model for the dimer-dimer interaction that involves an association between two beta-ribbon motifs. This model is supported by a fourth mutation that was predicted to disrupt the interaction and was shown to do so. Biochemical analyses of these mutants provide compelling evidence that cooperative protein-protein interaction between two dimers of RTP is essential to impose polar blocks to the elongation of both DNA and RNA chains.

Roma illustrata, or, A description of the most beautiful pieces of painting, sculpture, and architecture, antique and modern, at and near Rome.

Dedication signed by Robert Samber, who seems to have been the translator.

Grants-in-aid and other financial assistance programs administered by the U.S. Department of Health, Education, and Welfare.

Mode of access: Internet.

The glory that was Greece : a survey of Hellenic culture and civilisation /

Includes index.

Stratigraphy of the bituminous coal field of Pennsylvania, Ohio and West Virginia,

Mode of access: Internet.

Economic tendencies in the United States; aspects of pre-war and post-war changes,

"A publication of the National Bureau of Economic Research, inc., in cooperation with the Committee on Recent Economic Changes."

Mental retardation : activities of the U.S. Department of Health, Education, and Welfare.

Description based on: 1962.

The View from the tower : a collection of historical facts and anecdotal stories covering the early years of the Ohio Agricultural Research and Development Center /

Robert E. Whitmoyer, OARDC Historical Records Officer.

Tabulae atomicae : the chemical tables for the calculation of quantitative analyses of H. Rose. Recalculated for the more recent determinations of atomic weights, and with other alterations and additions /

Mode of access: Internet.

Account of the great learned societies and associations, and of the chief printing clubs of Great Britain and Ireland. By Bernard Quaritch.

Lists of club's members : [3] p. at end.

An inquiry into the history of slavery; its introduction into the United States; causes of its continuance; and remarks upon the abolition tracts of William E. Channing, D. D.

Mode of access: Internet.

Curiosities for the ingenious : selected from the most authentic treasures of nature, science and art, biography, history, and general literature.

Mode of access: Internet.