Tumor necrosis factor-alpha mediates changes in tissue protein turnover in a rat cancer cachexia model.

Rats bearing the Yoshida AH-130 ascites hepatoma showed enhanced fractional rates of protein degradation in gastrocnemius muscle, heart, and liver, while fractional synthesis rates were similar to those in non-tumor bearing rats. This hypercatabolic pattern was associated with marked perturbations of the hormonal homeostasis and presence of tumor necrosis factor in the circulation. The daily administration of a goat anti-murine TNF IgG to tumor-bearing rats decreased protein degradation rates in skeletal muscle, heart, and liver as compared with tumor-bearing rats receiving a nonimmune goat IgG. The anti-TNF treatment was also effective in attenuating early perturbations in insulin and corticosterone homeostasis. Although these results suggest that tumor necrosis factor plays a significant role in mediating the changes in protein turnover and hormone levels elicited by tumor growth, the inability of such treatment to prevent a reduction in body weight implies that other mediators or tumor-related events were also involved.

Structural quality of soils cultivated with coffee and pasture in an environmental protection area

Studies of soils in Environmental Protection Areas (EPAs) are of great importance, because they are an essential component of ecosystems, directly interfering in environmental sustainability. The objective of this study was to evaluate the structural quality of soil cultivated with coffee and used as pasture in the Capituva's River microbasin, which is located in the Environmental Protection Area in Coqueiral, south of the state of Minas Gerais. Uniaxial compression test (preconsolidation test) and soil resistance to penetration were used. Undisturbed samples were taken from the surface layer (0-5 cm) of the soils in the area: a typic dystrophic Red Latosol (LVd - Oxisol), a typic eutrophic Red Argisol (PVe - Ultisol), and a typic dystrophic Haplic Cambisol (CXbd - Inceptisol). A significant linear positive correlation was observed between the results of the preconsolidation test and soil resistance to penetration. Load bearing capacity of soil could be estimated accordingly by means of penetration resistance for LVd, PVe, and CXbd. Cambisol - CXbd showed lower loading support capacity and resistance to penetration than LVd and PVe, due to the better crop management in this soil that resulted in higher physical quality which accounts for higher production and environmental sustainability.

Structural sustainability of cambisol under different land use system

Incongruous management techniques have been associated with some significant loss of agricultural land to degradation in many parts of the world. Land degradation results in the alteration of physical, chemical and biological properties of the soil, thereby posing a serious threat to sustainable agricultural development. In this study, our objective is to evaluate the changes in a Cambisol structure under six land use systems using the load bearing capacity model. Sampling was conducted in Amazonas Region, Brazil, in the following land use: a) young secondary forest; b) old secondary forest; c) forest; d) pasture; e) cropping, and f) agroforestry. To obtain the load bearing capacity models the undisturbed soil samples were collected in those land use systems and subjected to the uniaxial compression test. These models were used to evaluate which land use system preserved or degraded the Cambisol structure. The results of the bulk density and total porosity of the soil samples were not adequate to quantify structural degradation in Cambisol. Using the forest topsoil level (0-0.03 m) as a reference, it was observed that pasture land use system was most severe in the degradation of the soil structure while the structure were most preserved under old secondary forest, cropping system and forest. At the subsoil level (0.10-0.13 m depth), the soil structure was most degraded in the cropping land use system while it was most preserved in young secondary forest and pasture. At the 0.20-0.23 m depth, soil structure degradation was most severe in the old secondary forest system and well preserved in young secondary forest, cropping and agroforestry.

Macadam Base Shoulders, HR-181, 1977

Highway safety and pavement performance can be directly influenced by the type of shoulders that are constructed. Shoulder design alternatives have always been rather limited. Moreover, the use of some of the alternatives has always been restricted by funding limitations. This research project seeks to explore the use of modified macadam base construction for shoulders. This type of shoulder design could offer the designer another option when paved or stabilized shoulders are being considered. Macadam base construction has in the past been shown to be quite strong and freedraining. Two macadam base shoulder designs were developed and constructed for this research project. A new roadway embankment and P.C.C. pavement were constructed on a section of US 6 east of Adel in Dallas County. The macadam base shoulders were constructed adjacent to the pavement as part of the project. The north shoulder was finished with a choke stone course and bituminous surface treatment and the south shoulder was finished with a two (2) inch layer of Type B Class II asphalt concrete. The initial results are considered satisfactory. Follow-up performance evaluation and load bearing tests are planned.

Tumor necrosis factor-alpha mediates changes in tissue protein turnover in a rat cancer cachexia model.

Rats bearing the Yoshida AH-130 ascites hepatoma showed enhanced fractional rates of protein degradation in gastrocnemius muscle, heart, and liver, while fractional synthesis rates were similar to those in non-tumor bearing rats. This hypercatabolic pattern was associated with marked perturbations of the hormonal homeostasis and presence of tumor necrosis factor in the circulation. The daily administration of a goat anti-murine TNF IgG to tumor-bearing rats decreased protein degradation rates in skeletal muscle, heart, and liver as compared with tumor-bearing rats receiving a nonimmune goat IgG. The anti-TNF treatment was also effective in attenuating early perturbations in insulin and corticosterone homeostasis. Although these results suggest that tumor necrosis factor plays a significant role in mediating the changes in protein turnover and hormone levels elicited by tumor growth, the inability of such treatment to prevent a reduction in body weight implies that other mediators or tumor-related events were also involved.

Effective notch method assessment of misalignment for fatigue loaded welds

 The objective of this thesis work was to assess axial misalignment in fatigue loaded welds using the effective notch method. As a result, the fatigue behaviour of non-load carrying cruciform fillet welded joint under cyclic tensile loading has been studied. Various degrees of axial misalignment have been found in one series of non-load carrying cruciform fillet welded joints used in a laboratory investigation. As a result, it was important to carry out a comprehensive investigation since axial misalignment forms part of thequality of fatigue loaded structure and can reduce the fatigue strength. To extend the study, the correlation between fatigue strength and stress ratio, as well as stress concentration factor, were also studied. Moreover, a closer investigation of place of crack initiation and its dependence on weld sequence and imperfections of test specimen (angular distortion) was studied. For the fatigue class calculations, FEM (finite element method) and the effectivenotch approach are used. The addressed variable is the axial misalignment whichis introduce by modeling the entire joint. Fracture mechanics based calculations are also used and quantitatively compared with effective notch and experimental results.

Statistical variation of weld profiles and their expected influence on fatigue strength

The general objective of this study was to conduct astatistical analysis on the variation of the weld profiles and their influence on the fatigue strength of the joint. Weld quality with respect to its fatigue strength is of importance which is the main concept behind this thesis. The intention of this study was to establish the influence of weld geometric parameters on the weld quality and fatigue strength. The effect of local geometrical variations of non-load carrying cruciform fillet welded joint under tensile loading wasstudied in this thesis work. Linear Elastic Fracture Mechanics was used to calculate fatigue strength of the cruciform fillet welded joints in as-welded condition and under cyclic tensile loading, for a range of weld geometries. With extreme value statistical analysis and LEFM, an attempt was made to relate the variation of the cruciform weld profiles such as weld angle and weld toe radius to respective FAT classes.

The influence ofvariable amplitude loading on the fatigue strength of rhs corners

Rectangular hollow section (RHS) members are components widely used in engineering applications because of their good-looking, good properties in engineering areas and inexpensive cost comparing to members with other sections. The increasing use of RHS in load bearing structures makes it necessary to analyze the fatigue behavior of the RHS members. In this thesis, concentration will be given to the fatigue behavior of the RHS members under variable amplitude pure torsional loading. For the RHS members, failure will normally occur in the corner region if the welded regions are under full penetration. This is because of the complicated stress components' distributions at the RHScorners, where all of three fracture mechanics modes will happen. Mode I is mainly caused by the residual stresses that caused by the manufacturing process. Modes II and III are caused by the applied torsional loading. Stress based Findleymodel is also used to analyze the stress components. Constant amplitude fatigue tests have been done as well as variable amplitude fatigue tests. The specimens under variable amplitude loading gave longer fatigue lives than those under constant amplitude loading. Results from tests show an S-N curvewith slope around 5.

Kosketukseton hitsin pintageometrian määrittäminen

Yleisesti tiedetään hitsin pintageometrian vaikuttavan rakenteen väsymislujuuteen. Nopean, edullisen ja luotettavan pintageometrian mittausmenetelmän kehittäminen on askel kohti tarkempaa ja varmempaa rakenteen väsymislujuuden tarkastelua. Tässä työssä on tutkittu hitsejä, joiden pinnan geometria on mitattu norjalaisen SINTEF -yrityksen kehittämällä rakenteellisen valon menetelmällä. Osana työtä kehitettiin MatLab -pohjainen ohjelma, jolla jälkikäsitellään mittauksesta saadut x-y-z -mittapisteet. Mittausdatan jälkikäsittelyssä saadaan mittauksesta määritettyähitsin reunan pyöristys, liittymäkulma, a-mitta, reunahaava ja kateettisuhde. Kehitettyä menetelmää käyttämällä mitattiin lähes 300 voimaakantamatontaristiliitoksen hitsiä. Mittaustuloksia verrattiin vastaavista kappaleista tehtyihin hiemittauksiin. Manuaalisen hieestä tehdyn mittauksen havaittiin olevan tarkempi ja pystyttiin havaitsemaan paikallisempia muotoja. Rakenteellisen valon mittauksissa tapahtunut heijastelu saatiin pienenemään käsittelemällä mitattava pinta mattavalkoisella maalilla. Rakenteellisen valon mittatarkkuudeksi saatiin noin 0,2 mm. Pohjautuen mitattuun hitsin reunan pyöristykseen ja liittymäkulmaan voidaan yksinkertaista kaavaa käyttämällä laskea hitsin jännityskonsentraatio ja näin saada alkuarvaus väsymislujuudelle. Myös muiden tekijöiden tiedetään vaikuttavan hitsin väsymislujuuteen, joten pyöristyksen ja liittymäkulman avulla tehdyt arviot eivät ole absoluuttisen oikeita. Tämä havaittiin väsytyskokeilla, joista yhdessä väsymisvaurio ei syntynyt suurimmankaan jännityskonsentraation alueella.

Constant and variable amplitude fatigue strength of high strength steel welds treated with ultrasonic impact treatment

Hitsattujen rakenteiden väsymiskestävyyttä pystytään parantamaan jälkikäsittelymenetelmillä, joistayksi, ultraäänikäsittely muokkaa hitsin geometriaa ja aiheuttaa puristusjäännösjännitystilan. Tässä tutkimuksessa verrataan kokeellisesti kuormaa kantamattoman hitsatun ja ui -käsitellyn rivan väsymislujuutta toisiinsa. Tutkimusohjelmaan kuuluu kahta teräslajia ja sekä vakio - että vaihtuva - amplitudista kuormitusta. Ultraäänikäsittelyllä saavutetaan väsymiskestoiän parantuminen vakio - ja vaihtuva - amplitudisella kuormituksella. Perusaineen lujuudella ei ole merkittää vaikutusta väsymislujuuteen kun liitos on hitsatussa tilassa. Tällöin väsymiskestävyyden määrää hitsin rajaviivan jännityskeskittymä. Ultraäänikäsitellyn hitsatunliitoksen väsymiskestävyys on suurempi korkeamman lujuuden omaavilla teräksillä. Tästä syystä korkealujuuksisten terästen käyttö ultraäänikäsiteltynä väsyttävästi kuormitetuissa kevytrakenteissa on perusteltua.

Fatigue Analysis of Welded Cruciform Joints with Cold Laps

Työssä on tutkittu vetojännityskuormituksen alaisena olevien hitsattujen kuormaa kantamattomien X-liitosten hitsin paikallisen geometrian variaation vaikutusta väsymislujuuteen. Muuttujina olivat reunan pyöristyssäde, kylmäjuoksun suuruus ja kylkikulma. Geometristen muuttujien parametrinen riippuvuussuhde on analysoitu usealla elementtimallilla. Väsymistarkastelu on suoritettu käyttämällä lineaaris-elastista murtumismekaniikkaa (LEFM) tasovenymätilassa ja materiaalina terästä. Särönkasvun suunnan ennustamisessaon käytetty maksimipääjännityskriteeriä sekä jännitysintensiteettikertoimet on määritetty J-integraalilla. Särön ydintymisvaihetta ei ole otettu huomioon. Rakenteen on oletettu olevan hitsatussa tilassa ja jännitysheilahdus on kokonaan tehollinen. Särön kasvunopeuden ennustamiseen on käytetty Paris'n lakia. Väsymislujuustulokset on esitetty karakteristisina väsymisluokkina (FAT) ja sovitettu parametriseksi yhtälöksi. Lopuksi väsymisanalyysin ennustamia tuloksia on verrattu saatavilla oleviin väsytystestituloksiin.

MIG-juotto väsymiskestävyyden parantamismenetelmänä

Tässä tutkimuksessa esitellään MIG-juoton periaatteet ja tarkastellaan menetelmää siitä näkökulmasta, että menetelmä voisi toimia yhtenä varteenotettavana hitsattujen liitosten väsymiskestävyyden parantamismenetelmänä. Muiden parantamismenetelmien laajaa kirjoa esitellään lyhyesti. MIG-juoton soveltuvuutta parantamismenetelmäksi tutkittiin väsytyskokein. Kokeiden avulla määritettiin pelkästään MIG-juotetun liitoksen väsymiskestävyys erilaisissa tapauksissa. Lisäksi määritettiin väsymiskestävyys tapauksessa, jossa perinteisen MAG-pienahitsin rajaviivalle MIG-juotetiin ylimääräinen palko. Jälkimmäisessä tapauksessa menetelmä toimii väsymiskestävyyden parantamismenetelmänä. Väsytyskokeita tehtiin kaikkiaan 63 kpl.Kaikkiaan erilaisia väsytyskoesarjoja oli kymmenen, joista kuusi oli 8-10 koekappaletta käsittäviä varsinaisia tutkittavia tapauksia ja neljä pieniä, vain kaksi tai kolme koesauvaa käsittäviä pilottikoesarjoja. Koetuloksia verrattiin IIW:n(International Institute of Welding) suunnitteluohjeisiin ja suosituksiin. MIG-juotettujen liitosten väsymiskestävyys oli yleisesti ottaen hyvä. Päittäisliitosoli vähintään yhtä hyvä kuin tavanomaisesti hitsattu. Voimaa kantavassa taivutustapauksessa saatiin 110 % parempi väsymislujuus verrattuna IIW:n suunnitteluohjeeseen. Voimaa kantamattomissa poikittaisissa kiinnitysliitoksissa puolestaan saavutettiin paikallisia nimellisiä jännityksiä käyttäen 70-80 %:n parannus suunnitteluohjeeseen nähden. Pelkästään MIG-juotetun liitoksen ja 'parannetun' liitoksen väsymislujuudet olivat tässä tapauksessa likimain yhtä suuret. MIG-juottoa käytetään vaihtoehtoisena liittämismenetelmänä jo joissakin teollisissa sovelluksissa. Tämän tutkimuksen koetulokset antavat selviä viitteitä siitä, että menetelmä voisi toimia yhtenä varteenotettavana väsymiskestävyyden parantamiskeinona uusille tai korjatuille rakenteille. Vaaditaan kuitenkin vielä lisätutkimuksia, jotta menetelmää voisi soveltaa luottavaisin mielin tuotantokäytössä väsymiskestävyyden parantamismenetelmänä.

A novel approachfor assessing the fatigue strength of ultrasonic impact treated welded structures.

It is commonly observed that complex fabricated structures subject tofatigue loading fail at the welded joints. Some problems can be corrected by proper detail design but fatigue performance can also be improved using post-weld improvement methods. In general, improvement methods can be divided into two main groups: weld geometry modification methods and residual stress modification methods. The former remove weld toe defects and/or reduce the stress concentrationwhile the latter introduce compressive stress fields in the area where fatigue cracks are likely to initiate. Ultrasonic impact treatment (UIT) is a novel post-weld treatment method that influences both the residual stress distribution andimproves the local geometry of the weld. The structural fatigue strength of non-load carrying attachments in the as-welded condition has been experimentally compared to the structural fatigue strength of ultrasonic impact treated welds. Longitudinal attachment specimens made of two thicknesses of steel S355 J0 have been tested for determining the efficiency of ultrasonic impacttreatment. Treated welds were found to have about 50% greater structural fatigue strength, when the slope of the S-N-curve is three. High mean stress fatigue testing based on the Ohta-method decreased the degree of weld improvement only 19%. This indicated that the method could be also applied for large fabricated structures operating under high reactive residual stresses equilibrated within the volume of the structure. The thickness of specimens has no significant effect tothe structural fatigue strength. The fatigue class difference between 5 mm and 8 mm specimen was only 8%. It was hypothesized that the UIT method added a significant crack initiation period to the total fatigue life of the welded joints. Crack initiation life was estimated by a local strain approach. Material parameters were defined using a modified Uniform Material Law developed in Germany. Finite element analysis and X-ray diffraction were used to define, respectively, the stress concentration and mean stress. The theoretical fatigue life was found to have good accuracy comparing to experimental fatigue tests.The predictive behaviour of the local strain approach combined with the uniformmaterial law was excellent for the joint types and conditions studied in this work.

Modeling dynamic behavior in tilting pad gas journal bearings

Thisresearch deals with the dynamic modeling of gas lubricated tilting pad journal bearings provided with spring supported pads, including experimental verification of the computation. On the basis of a mathematical model of a film bearing, a computer program has been developed, which can be used for the simulation of a special type of tilting pad gas journal bearing supported by a rotary spring under different loading conditions time dependently (transient running conditions due to geometry variations in time externally imposed). On the basis of literature, different transformations have been used in the model to achieve simpler calculation. The numerical simulation is used to solve a non-stationary case of a gasfilm. The simulation results were compared with literature results in a stationary case (steady running conditions) and they were found to be equal. In addition to this, comparisons were made with a number of stationary and non-stationary bearing tests, which were performed at Lappeenranta University of Technology using bearings designed with the simulation program. A study was also made using numerical simulation and literature to establish the influence of the different bearing parameters on the stability of the bearing. Comparison work was done with literature on tilting pad gas bearings. This bearing type is rarely used. One literature reference has studied the same bearing type as that used in LUT. A new design of tilting pad gas bearing is introduced. It is based on a stainless steel body and electron beam welding of the bearing parts. It has good operation characteristics and is easier to tune and faster to manufacture than traditional constructions. It is also suitable for large serial production.

Fibre-reinforced composites as root canal posts

Fibre-reinforced composite (FRC) root canal posts are suggested to have biomechanical benefits over traditional metallic posts, but they lack good adhesion to resin composites. The aim of this series of studies was to evaluate the adhesion of individually formed fibre-reinforced composite material to composite resin and dentin, as well as some mechanical properties. Flexural properties were evaluated and compared between individually formed FRC post material and different prefabricated posts. The depth of polymerization of the individually formed FRC post material was evaluated with IR spectrophotometry and microhardness measurements, and compared to that of resin without fibres. Bonding properties of the individually formed FRC post to resin cements and dentin were tested using Pull-out- and Push-out-force tests, evaluated with scanning electron microscopy, and compared to those of prefabricated FRC and metal posts. Load-bearing capacity and microstrain were evaluated and failure mode assessment was made on incisors restored with individually formed FRC posts of different structures and prefabricated posts. The results of these studies show that the individually polymerized and formed FRC post material had higher flexural properties compared to the commercial prefabricated FRC posts. The individually polymerized FRC material showed almost the same degree of conversion after light polymerization as monomer resin without fibres. Moreover, it was found that the individually formed FRC post material with a semiinterpenetrating polymer network (IPN) polymer matrix bonded better to composite resin luting cement, than did the prefabricated posts with a cross-linked polymer matrix. Furthermore, it was found that, contrary to the other posts, there were no adhesive failures between the individually formed FRC posts and composite resin luting cement. This suggests better interfacial adhesion of cements to these posts. Although no differences in load-bearing capacity or microstrain could be seen, the incisors restored with individually formed FRC posts with a hollow structure showed more favourable failures compared to other prefabricated posts. These studies suggest that it is possible to use individually formed FRC material with semi-IPN polymer matrix as root canal post material. They also indicate that there are benefits especially regarding the bonding properties to composite resin and dentin with this material compared to prefabricated FRC post material with a cross-linked matrix. Furthermore, clinically more repairable failures were found with this material compared to those of prefabricated posts.